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The map label for this gene is rnhA

Identifier: 37680714

GI number: 37680714

Start: 2560072

End: 2560539

Strand: Direct

Name: rnhA

Synonym: VV2530

Alternate gene names: 37680714

Gene position: 2560072-2560539 (Clockwise)

Preceding gene: 37680709

Following gene: 37680716

Centisome position: 76.32

GC content: 48.5

Gene sequence:

>468_bases
ATGACAAAACAAGTTGAAATTTTCACTGACGGTTCTTGCCTCGGTAATCCTGGACCCGGTGGTTACGGTGTCGTCTTGCG
TTACAAACAGGTCGAAAAAACCTTAGCACAAGGCTATAGACTCACCACCAACAACCGAATGGAAATGATGGCCACCATCG
TTGCTCTTCAGGCCTTGAAAGAACCTTGCAATGTGATTCTCACAACCGATAGCCAATATGTTCGACAAGGTATTACTCAG
TGGATCCACAATTGGAAAAAGCGCGGTTGGAAAACCGCCGATAAAAAACCAGTCAAAAATGCCGATCTTTGGCAAGCGCT
TGATAAAGAAACCACTCGCCATACGATTGACTGGCGTTGGGTCAAAGGACACGCAGGCCATCGTGAAAACGAAATGTGTG
ACGAGTTGGCGCGTGCCGCGGCCGAAAACCCAACCCTAGACGACACTGGCTATCAGCCTGCCGAGTAA

Upstream 100 bases:

>100_bases
CGATCGTATTCAGAATTGTTACTGGTATTCATAATGATATGTGTGTCAGACTATGCAGAACGTATGCTTTAAACATGGCT
TTCGCTAATAGTACCAAAAC

Downstream 100 bases:

>100_bases
ACTACTCTCTTCCTCTGCAAGCGCAGCTTTTGCGCTTGCTCAGTAAATCACGCTGTCGGTTCATTACCCCGAGCGGAGAT
AAACGACGCTTTAACTGCCA

Product: ribonuclease H

Products: NA

Alternate protein names: RNase HI

Number of amino acids: Translated: 155; Mature: 154

Protein sequence:

>155_residues
MTKQVEIFTDGSCLGNPGPGGYGVVLRYKQVEKTLAQGYRLTTNNRMEMMATIVALQALKEPCNVILTTDSQYVRQGITQ
WIHNWKKRGWKTADKKPVKNADLWQALDKETTRHTIDWRWVKGHAGHRENEMCDELARAAAENPTLDDTGYQPAE

Sequences:

>Translated_155_residues
MTKQVEIFTDGSCLGNPGPGGYGVVLRYKQVEKTLAQGYRLTTNNRMEMMATIVALQALKEPCNVILTTDSQYVRQGITQ
WIHNWKKRGWKTADKKPVKNADLWQALDKETTRHTIDWRWVKGHAGHRENEMCDELARAAAENPTLDDTGYQPAE
>Mature_154_residues
TKQVEIFTDGSCLGNPGPGGYGVVLRYKQVEKTLAQGYRLTTNNRMEMMATIVALQALKEPCNVILTTDSQYVRQGITQW
IHNWKKRGWKTADKKPVKNADLWQALDKETTRHTIDWRWVKGHAGHRENEMCDELARAAAENPTLDDTGYQPAE

Specific function: Endonuclease that specifically degrades the RNA of RNA- DNA hybrids

COG id: COG0328

COG function: function code L; Ribonuclease HI

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 RNase H domain

Homologues:

Organism=Homo sapiens, GI21359816, Length=147, Percent_Identity=37.4149659863946, Blast_Score=77, Evalue=6e-15,
Organism=Escherichia coli, GI1786408, Length=152, Percent_Identity=79.6052631578947, Blast_Score=257, Evalue=2e-70,
Organism=Caenorhabditis elegans, GI115532542, Length=141, Percent_Identity=34.0425531914894, Blast_Score=81, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17505482, Length=133, Percent_Identity=34.5864661654135, Blast_Score=69, Evalue=1e-12,
Organism=Drosophila melanogaster, GI17137476, Length=144, Percent_Identity=36.8055555555556, Blast_Score=86, Evalue=8e-18,
Organism=Drosophila melanogaster, GI45552509, Length=144, Percent_Identity=36.8055555555556, Blast_Score=86, Evalue=9e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RNH_VIBVU (Q8DBD5)

Other databases:

- EMBL:   AE016795
- RefSeq:   NP_760761.1
- ProteinModelPortal:   Q8DBD5
- SMR:   Q8DBD5
- GeneID:   1178787
- GenomeReviews:   AE016795_GR
- KEGG:   vvu:VV1_1886
- HOGENOM:   HBG742437
- OMA:   NGWRTAD
- ProtClustDB:   PRK00203
- BioCyc:   VVUL216895:VV1_1886-MONOMER
- BRENDA:   3.1.26.4
- GO:   GO:0005737
- HAMAP:   MF_00042
- InterPro:   IPR022892
- InterPro:   IPR012337
- InterPro:   IPR002156

Pfam domain/function: PF00075 RnaseH; SSF53098 RNaseH_fold

EC number: =3.1.26.4

Molecular weight: Translated: 17647; Mature: 17516

Theoretical pI: Translated: 8.47; Mature: 8.47

Prosite motif: PS50879 RNASE_H

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKQVEIFTDGSCLGNPGPGGYGVVLRYKQVEKTLAQGYRLTTNNRMEMMATIVALQALK
CCCEEEEEECCCCCCCCCCCCCEEEEEHHHHHHHHHCCEECCCCCHHHHHHHHHHHHHHH
EPCNVILTTDSQYVRQGITQWIHNWKKRGWKTADKKPVKNADLWQALDKETTRHTIDWRW
CCCCEEEECCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHCCCEEEE
VKGHAGHRENEMCDELARAAAENPTLDDTGYQPAE
EECCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCC
>Mature Secondary Structure 
TKQVEIFTDGSCLGNPGPGGYGVVLRYKQVEKTLAQGYRLTTNNRMEMMATIVALQALK
CCEEEEEECCCCCCCCCCCCCEEEEEHHHHHHHHHCCEECCCCCHHHHHHHHHHHHHHH
EPCNVILTTDSQYVRQGITQWIHNWKKRGWKTADKKPVKNADLWQALDKETTRHTIDWRW
CCCCEEEECCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHCCCEEEE
VKGHAGHRENEMCDELARAAAENPTLDDTGYQPAE
EECCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA