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The map label for this gene is gloB2

Identifier: 37680716

GI number: 37680716

Start: 2561347

End: 2562105

Strand: Direct

Name: gloB2

Synonym: VV2532

Alternate gene names: 37680716

Gene position: 2561347-2562105 (Clockwise)

Preceding gene: 37680714

Following gene: 37680717

Centisome position: 76.36

GC content: 47.17

Gene sequence:

>759_bases
GTGTTAGAGATCAAAAGCATACCTGCATTCAACGACAATTACATCTGGTTGATTCAAAATAGCGATCAGCGTTGTGCTGT
GGTCGACCCAGGCGACGCCAAGCCTGTCCTTCACTACATCGAGCAACATCAGTTAACTTTAGAAGCGATTCTTATCACCC
ACCACCACAATGATCACATTGGCGGCGTGGCCGATTTGGTGCGCGCTTTTCCTAACGTTAACGTTGTAGGGCCGAAAGCA
GAACCGATTCCAACGCTCACCCACCCCGTGGAAGATGGCGACCGACTTGAGTTATTCGACGAAACCTTCTTAGTATTAGG
TTTAGGTGGGCATACCTTGGGTCATATTGGTTATGTTGGCGACGGCAAACTGTTTTGTGGCGATGTACTCTTCTCTGCTG
GCTGTGGTCGTATTTTTGAAGGTACCGCGCAACAAATGTTTGACTCACTCAACAAGCTTCTCGCCCTTCCTGAGGAAACG
GAAGTGTTCTGCGCTCATGAATATACCGCCAGCAACGTTGCTTTCGCCTTAGCCGTGGAACCGGATAACGAGTTACTTCA
CCAATACCGCGATACCGTCAATCGACTGCGTGCGCAAAATCTGCCAACCATTCCAACCACATTAAGACAAGAAAAATGGA
TCAATCCATTTCTTCGATATCTGCAACCCAGTGTTATTCACTCGGTTTCTAGCAGAACGAAAAATAGCGATCCACTCTCG
GTTTTTACAGCATTACGTGAGTGGAAGAACGAATTTTAA

Upstream 100 bases:

>100_bases
TTGTTCATTTCTTCCCATGTATAGGGATGCTGGATAACTTTACTGCTTAGTGCTGGCTTCATTAACCACCTGACTAGTTC
GAACGAGCGGAGAATCCATC

Downstream 100 bases:

>100_bases
CTTTTTGATGCTTGTCTCCTGACCAAGGAGGCAAGTATTATCAGCAGCCCAAAGTTAAACAGGCTGGAACCATGCGTTTT
AAGTTTAGTTGGGTGTTTGC

Product: putative hydroxyacylglutathione hydrolase GloB

Products: NA

Alternate protein names: Glyoxalase II 2; Glx II 2

Number of amino acids: Translated: 252; Mature: 252

Protein sequence:

>252_residues
MLEIKSIPAFNDNYIWLIQNSDQRCAVVDPGDAKPVLHYIEQHQLTLEAILITHHHNDHIGGVADLVRAFPNVNVVGPKA
EPIPTLTHPVEDGDRLELFDETFLVLGLGGHTLGHIGYVGDGKLFCGDVLFSAGCGRIFEGTAQQMFDSLNKLLALPEET
EVFCAHEYTASNVAFALAVEPDNELLHQYRDTVNRLRAQNLPTIPTTLRQEKWINPFLRYLQPSVIHSVSSRTKNSDPLS
VFTALREWKNEF

Sequences:

>Translated_252_residues
MLEIKSIPAFNDNYIWLIQNSDQRCAVVDPGDAKPVLHYIEQHQLTLEAILITHHHNDHIGGVADLVRAFPNVNVVGPKA
EPIPTLTHPVEDGDRLELFDETFLVLGLGGHTLGHIGYVGDGKLFCGDVLFSAGCGRIFEGTAQQMFDSLNKLLALPEET
EVFCAHEYTASNVAFALAVEPDNELLHQYRDTVNRLRAQNLPTIPTTLRQEKWINPFLRYLQPSVIHSVSSRTKNSDPLS
VFTALREWKNEF
>Mature_252_residues
MLEIKSIPAFNDNYIWLIQNSDQRCAVVDPGDAKPVLHYIEQHQLTLEAILITHHHNDHIGGVADLVRAFPNVNVVGPKA
EPIPTLTHPVEDGDRLELFDETFLVLGLGGHTLGHIGYVGDGKLFCGDVLFSAGCGRIFEGTAQQMFDSLNKLLALPEET
EVFCAHEYTASNVAFALAVEPDNELLHQYRDTVNRLRAQNLPTIPTTLRQEKWINPFLRYLQPSVIHSVSSRTKNSDPLS
VFTALREWKNEF

Specific function: Thiolesterase that catalyzes the hydrolysis of S-D- lactoyl-glutathione to form glutathione and D-lactic acid

COG id: COG0491

COG function: function code R; Zn-dependent hydrolases, including glyoxylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the metallo-beta-lactamase superfamily. Glyoxalase II family

Homologues:

Organism=Homo sapiens, GI116642887, Length=226, Percent_Identity=40.7079646017699, Blast_Score=159, Evalue=2e-39,
Organism=Homo sapiens, GI21703352, Length=226, Percent_Identity=40.7079646017699, Blast_Score=159, Evalue=3e-39,
Organism=Homo sapiens, GI14150041, Length=259, Percent_Identity=37.0656370656371, Blast_Score=151, Evalue=6e-37,
Organism=Homo sapiens, GI94538320, Length=260, Percent_Identity=34.2307692307692, Blast_Score=149, Evalue=2e-36,
Organism=Homo sapiens, GI94538322, Length=260, Percent_Identity=34.2307692307692, Blast_Score=149, Evalue=2e-36,
Organism=Homo sapiens, GI46361987, Length=191, Percent_Identity=40.8376963350785, Blast_Score=127, Evalue=8e-30,
Organism=Escherichia coli, GI1786406, Length=251, Percent_Identity=47.0119521912351, Blast_Score=221, Evalue=3e-59,
Organism=Caenorhabditis elegans, GI17536925, Length=264, Percent_Identity=35.9848484848485, Blast_Score=155, Evalue=2e-38,
Organism=Saccharomyces cerevisiae, GI6320478, Length=263, Percent_Identity=29.277566539924, Blast_Score=104, Evalue=2e-23,
Organism=Saccharomyces cerevisiae, GI6324614, Length=260, Percent_Identity=28.4615384615385, Blast_Score=84, Evalue=2e-17,
Organism=Drosophila melanogaster, GI21356335, Length=260, Percent_Identity=39.6153846153846, Blast_Score=183, Evalue=1e-46,
Organism=Drosophila melanogaster, GI24667711, Length=260, Percent_Identity=39.6153846153846, Blast_Score=182, Evalue=1e-46,
Organism=Drosophila melanogaster, GI24667703, Length=260, Percent_Identity=39.6153846153846, Blast_Score=182, Evalue=2e-46,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GLO22_VIBVY (Q7MII4)

Other databases:

- EMBL:   BA000037
- RefSeq:   NP_935325.1
- HSSP:   Q9SID3
- ProteinModelPortal:   Q7MII4
- SMR:   Q7MII4
- STRING:   Q7MII4
- GeneID:   2625340
- GenomeReviews:   BA000037_GR
- KEGG:   vvy:VV2532
- eggNOG:   COG0491
- HOGENOM:   HBG753931
- OMA:   WCAHEYT
- ProtClustDB:   CLSK874727
- BioCyc:   VVUL196600:VV2532-MONOMER
- HAMAP:   MF_01374
- InterPro:   IPR001279
- InterPro:   IPR017782
- SMART:   SM00849
- TIGRFAMs:   TIGR03413

Pfam domain/function: PF00753 Lactamase_B

EC number: =3.1.2.6

Molecular weight: Translated: 28248; Mature: 28248

Theoretical pI: Translated: 5.28; Mature: 5.28

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLEIKSIPAFNDNYIWLIQNSDQRCAVVDPGDAKPVLHYIEQHQLTLEAILITHHHNDHI
CCEECCCCCCCCCEEEEEECCCCEEEEECCCCCHHHHHHHHHHHHEEEEEEEEECCCCCC
GGVADLVRAFPNVNVVGPKAEPIPTLTHPVEDGDRLELFDETFLVLGLGGHTLGHIGYVG
CHHHHHHHHCCCCEEECCCCCCCCCCCCCCCCCCCEEECCCEEEEEECCCCHHCCEEEEC
DGKLFCGDVLFSAGCGRIFEGTAQQMFDSLNKLLALPEETEVFCAHEYTASNVAFALAVE
CCCEEHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCEEEEEEEC
PDNELLHQYRDTVNRLRAQNLPTIPTTLRQEKWINPFLRYLQPSVIHSVSSRTKNSDPLS
CCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCHHHHHHHHHCCCCCCHHH
VFTALREWKNEF
HHHHHHHHHHCC
>Mature Secondary Structure
MLEIKSIPAFNDNYIWLIQNSDQRCAVVDPGDAKPVLHYIEQHQLTLEAILITHHHNDHI
CCEECCCCCCCCCEEEEEECCCCEEEEECCCCCHHHHHHHHHHHHEEEEEEEEECCCCCC
GGVADLVRAFPNVNVVGPKAEPIPTLTHPVEDGDRLELFDETFLVLGLGGHTLGHIGYVG
CHHHHHHHHCCCCEEECCCCCCCCCCCCCCCCCCCEEECCCEEEEEECCCCHHCCEEEEC
DGKLFCGDVLFSAGCGRIFEGTAQQMFDSLNKLLALPEETEVFCAHEYTASNVAFALAVE
CCCEEHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCEEEEEEEC
PDNELLHQYRDTVNRLRAQNLPTIPTTLRQEKWINPFLRYLQPSVIHSVSSRTKNSDPLS
CCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCHHHHHHHHHCCCCCCHHH
VFTALREWKNEF
HHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA