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The map label for this gene is purR

Identifier: 37680489

GI number: 37680489

Start: 2326705

End: 2327709

Strand: Direct

Name: purR

Synonym: VV2305

Alternate gene names: 37680489

Gene position: 2326705-2327709 (Clockwise)

Preceding gene: 37680488

Following gene: 37680490

Centisome position: 69.36

GC content: 46.67

Gene sequence:

>1005_bases
ATGGCCACAATTAAAGATGTCGCGCGTCTCGCTGGAGTATCCACGACCACAGTGTCGCACGTAATCAACAAAACGCGTTT
TGTTGCAGAAGCCACTCAAGAAAAAGTCATGAAAGCGGTTGACGAGCTCAACTACGCACCGAGCGCAGTAGCGCGTAGCC
TAAAGTGCAACACCACTCGTACTATTGGTATGCTGGTCACACAGTCGACCAATTTGTTCTTTTCTGAAGTGATTGATGGC
GTAGAAAGCTACTGTTACCGCCAAGGCTATACGCTAATTCTTTGTAATACAGGCGGTATCTACGAGAAGCAACGTGACTA
CATTCGTATGCTTGCGGAAAAGCGCGTAGATGGCATTTTGGTGATGTGTTCAGACCTCACCGAAGAGCTCCGCGAAATGC
TTGATAAGCATGCAGATATTCCTAAAGTGATTATGGACTGGGGTCCAATCAGCTCACAAGCCGATAAAATTATCGATAAC
TCAGAAGAAGGTGGCTACTTGGCCACTAAGTACCTGATTGATAATGGCCATACAAAAATCGCATGTTTGAGTGGCCACTT
TGAAAAAGCCGCGTGCCAAGAGCGCATCCAAGGCTTTAAACGTGCGATGAAGGAAGCCAATATCACACTAAACAACGAGT
GGATCCTTGAAGGAAACTTCGAATGTGACACGGCTGTGATGGCTGCTGACAAGATCGCGTCGTGGCAAGATCGCCCAACG
GCGGTTTTCTGCTTCAACGATACCATGGCACTGGGTTTGATGAGCCGCCTTCAACAGTTAGGCCTTCGCATTCCTGAAGA
CATCTCTGTGATTGGCTACGATAATATTGAGTTAGCAGAGTACTTCTCACCACCGCTAACAACGATCCACCAGCCAAAAC
GCCGTGTTGGTAAAAATGCATTTGAGATCCTTCTCGAACGCATTAAGGACAAAGAACATGACAAACGCGTGTTTGAAATG
CATCCCGAGTTAGTGGTTCGCGATACCGTTAAAAAAATCAATTAG

Upstream 100 bases:

>100_bases
AGTGTGTCTTCCTTACAAAGCGAATTCGGAGAAAGGTAGCAAACTGTGTTGCAACAAAGAATCGTAGATTCAAAATGCAA
CCTTAGACAGGACAATACCT

Downstream 100 bases:

>100_bases
TTAAGACTAATATTCTAAAGCATCCAAATCTGGATGCTTTTTTTTCATTCTTAGTAAGTGATTTGTTAAGTCAAATTCTG
TTAAGTTCTTAAAATCGAGC

Product: transcriptional regulator

Products: NA

Alternate protein names: Pur regulon repressor; Purine nucleotide synthesis repressor

Number of amino acids: Translated: 334; Mature: 333

Protein sequence:

>334_residues
MATIKDVARLAGVSTTTVSHVINKTRFVAEATQEKVMKAVDELNYAPSAVARSLKCNTTRTIGMLVTQSTNLFFSEVIDG
VESYCYRQGYTLILCNTGGIYEKQRDYIRMLAEKRVDGILVMCSDLTEELREMLDKHADIPKVIMDWGPISSQADKIIDN
SEEGGYLATKYLIDNGHTKIACLSGHFEKAACQERIQGFKRAMKEANITLNNEWILEGNFECDTAVMAADKIASWQDRPT
AVFCFNDTMALGLMSRLQQLGLRIPEDISVIGYDNIELAEYFSPPLTTIHQPKRRVGKNAFEILLERIKDKEHDKRVFEM
HPELVVRDTVKKIN

Sequences:

>Translated_334_residues
MATIKDVARLAGVSTTTVSHVINKTRFVAEATQEKVMKAVDELNYAPSAVARSLKCNTTRTIGMLVTQSTNLFFSEVIDG
VESYCYRQGYTLILCNTGGIYEKQRDYIRMLAEKRVDGILVMCSDLTEELREMLDKHADIPKVIMDWGPISSQADKIIDN
SEEGGYLATKYLIDNGHTKIACLSGHFEKAACQERIQGFKRAMKEANITLNNEWILEGNFECDTAVMAADKIASWQDRPT
AVFCFNDTMALGLMSRLQQLGLRIPEDISVIGYDNIELAEYFSPPLTTIHQPKRRVGKNAFEILLERIKDKEHDKRVFEM
HPELVVRDTVKKIN
>Mature_333_residues
ATIKDVARLAGVSTTTVSHVINKTRFVAEATQEKVMKAVDELNYAPSAVARSLKCNTTRTIGMLVTQSTNLFFSEVIDGV
ESYCYRQGYTLILCNTGGIYEKQRDYIRMLAEKRVDGILVMCSDLTEELREMLDKHADIPKVIMDWGPISSQADKIIDNS
EEGGYLATKYLIDNGHTKIACLSGHFEKAACQERIQGFKRAMKEANITLNNEWILEGNFECDTAVMAADKIASWQDRPTA
VFCFNDTMALGLMSRLQQLGLRIPEDISVIGYDNIELAEYFSPPLTTIHQPKRRVGKNAFEILLERIKDKEHDKRVFEMH
PELVVRDTVKKIN

Specific function: Is the main repressor of the genes involved in the de novo synthesis of purine nucleotides, regulating purB, purC, purEK, purF, purHD, purL, purMN and guaBA expression. PurR is allosterically activated to bind its cognate DNA by binding the purine corepre

COG id: COG1609

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain

Homologues:

Organism=Escherichia coli, GI1787948, Length=331, Percent_Identity=51.6616314199396, Blast_Score=360, Evalue=1e-100,
Organism=Escherichia coli, GI1790194, Length=329, Percent_Identity=48.936170212766, Blast_Score=340, Evalue=1e-94,
Organism=Escherichia coli, GI1790369, Length=334, Percent_Identity=32.9341317365269, Blast_Score=213, Evalue=2e-56,
Organism=Escherichia coli, GI1788474, Length=333, Percent_Identity=32.7327327327327, Blast_Score=167, Evalue=1e-42,
Organism=Escherichia coli, GI1787580, Length=323, Percent_Identity=30.6501547987616, Blast_Score=166, Evalue=2e-42,
Organism=Escherichia coli, GI1789202, Length=298, Percent_Identity=33.2214765100671, Blast_Score=165, Evalue=4e-42,
Organism=Escherichia coli, GI1789068, Length=295, Percent_Identity=29.4915254237288, Blast_Score=154, Evalue=8e-39,
Organism=Escherichia coli, GI1786540, Length=309, Percent_Identity=30.0970873786408, Blast_Score=133, Evalue=2e-32,
Organism=Escherichia coli, GI48994940, Length=318, Percent_Identity=26.7295597484277, Blast_Score=120, Evalue=1e-28,
Organism=Escherichia coli, GI1787906, Length=324, Percent_Identity=25.3086419753086, Blast_Score=115, Evalue=4e-27,
Organism=Escherichia coli, GI1790715, Length=325, Percent_Identity=25.8461538461538, Blast_Score=110, Evalue=2e-25,
Organism=Escherichia coli, GI1786268, Length=315, Percent_Identity=24.7619047619048, Blast_Score=102, Evalue=4e-23,
Organism=Escherichia coli, GI1789456, Length=323, Percent_Identity=25.6965944272446, Blast_Score=96, Evalue=3e-21,
Organism=Escherichia coli, GI1790689, Length=333, Percent_Identity=21.6216216216216, Blast_Score=79, Evalue=6e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PURR_VIBVU (Q8DAQ5)

Other databases:

- EMBL:   AE016795
- RefSeq:   NP_760996.1
- HSSP:   P03023
- ProteinModelPortal:   Q8DAQ5
- SMR:   Q8DAQ5
- GeneID:   1179023
- GenomeReviews:   AE016795_GR
- KEGG:   vvu:VV1_2138
- HOGENOM:   HBG753640
- OMA:   DWGPESS
- ProtClustDB:   CLSK874518
- BioCyc:   VVUL216895:VV1_2138-MONOMER
- GO:   GO:0005622
- HAMAP:   MF_01277
- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761
- PRINTS:   PR00036
- SMART:   SM00354

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1; SSF47413 Lambda_like_DNA

EC number: NA

Molecular weight: Translated: 37757; Mature: 37626

Theoretical pI: Translated: 6.18; Mature: 6.18

Prosite motif: PS00356 HTH_LACI_1; PS50932 HTH_LACI_2

Important sites: BINDING 73-73 BINDING 189-189 BINDING 220-220 BINDING 274-274

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATIKDVARLAGVSTTTVSHVINKTRFVAEATQEKVMKAVDELNYAPSAVARSLKCNTTR
CCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCE
TIGMLVTQSTNLFFSEVIDGVESYCYRQGYTLILCNTGGIYEKQRDYIRMLAEKRVDGIL
EEEEEEECCCHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHCCCCEE
VMCSDLTEELREMLDKHADIPKVIMDWGPISSQADKIIDNSEEGGYLATKYLIDNGHTKI
EEHHHHHHHHHHHHHHCCCCCHHHHCCCCCCHHHHHHHCCCCCCCEEEEEEEEECCCEEE
ACLSGHFEKAACQERIQGFKRAMKEANITLNNEWILEGNFECDTAVMAADKIASWQDRPT
EEECCCCHHHHHHHHHHHHHHHHHHCCCEECCEEEEECCCCCCHHHHHHHHHHCCCCCCC
AVFCFNDTMALGLMSRLQQLGLRIPEDISVIGYDNIELAEYFSPPLTTIHQPKRRVGKNA
EEEEECCHHHHHHHHHHHHHCCCCCCCCEEEECCCCHHHHHCCCCHHHHCCHHHHHCHHH
FEILLERIKDKEHDKRVFEMHPELVVRDTVKKIN
HHHHHHHHHCCHHHHHHHHHCCHHHHHHHHHHCC
>Mature Secondary Structure 
ATIKDVARLAGVSTTTVSHVINKTRFVAEATQEKVMKAVDELNYAPSAVARSLKCNTTR
CCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCE
TIGMLVTQSTNLFFSEVIDGVESYCYRQGYTLILCNTGGIYEKQRDYIRMLAEKRVDGIL
EEEEEEECCCHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHCCCCEE
VMCSDLTEELREMLDKHADIPKVIMDWGPISSQADKIIDNSEEGGYLATKYLIDNGHTKI
EEHHHHHHHHHHHHHHCCCCCHHHHCCCCCCHHHHHHHCCCCCCCEEEEEEEEECCCEEE
ACLSGHFEKAACQERIQGFKRAMKEANITLNNEWILEGNFECDTAVMAADKIASWQDRPT
EEECCCCHHHHHHHHHHHHHHHHHHCCCEECCEEEEECCCCCCHHHHHHHHHHCCCCCCC
AVFCFNDTMALGLMSRLQQLGLRIPEDISVIGYDNIELAEYFSPPLTTIHQPKRRVGKNA
EEEEECCHHHHHHHHHHHHHCCCCCCCCEEEECCCCHHHHHCCCCHHHHCCHHHHHCHHH
FEILLERIKDKEHDKRVFEMHPELVVRDTVKKIN
HHHHHHHHHCCHHHHHHHHHCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA