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The map label for this gene is emrD

Identifier: 37680172

GI number: 37680172

Start: 1986386

End: 1987633

Strand: Direct

Name: emrD

Synonym: VV1988

Alternate gene names: 37680172

Gene position: 1986386-1987633 (Clockwise)

Preceding gene: 37680171

Following gene: 37680177

Centisome position: 59.22

GC content: 50.64

Gene sequence:

>1248_bases
ATGGTCTTTTTCGTTTTTAATTTAGCCGGAGAATTTGCGCAGATGTCCGCGTCATTTCCATTAGCCAAGCTTACCTTTTT
GATCGCTATTTTGACCGCTGTGGGTCAAATGACTCAAACCATGTACGTGCCGTCAATCGGTCACATGGCCAGTGAGTTTT
TGGTATCGCCTGCTGCACTGCAGGCCGTCATGGCGTGCTATTTGATCCCTTACGGATTATCGCAATTTGTCTATGGTCCA
CTGTCGGATCGCCTTGGCCGTAAGCCGATCATCATCGCCGGTCTATTGATTTACATTGCCGGCACTATGCTGTCACTGTT
TGCTCACGAGTACACTTGGTTCCTTGTCGGTAGTTTCGTTCAAGGCTTGGGGATCGGTTGTGGTGGTGCAATGTCTCGCA
CCTTGACTCGTGACTGTTTTGAAGGCGCTGAATTACATCGCGCCAACAGTTTGATCAGCATGTGCGTGATCTTTTCACCG
CTGATGGCTCCTGTATTGGGCGGCCTGCTAACGGAAACCTTTGGCTGGCGTTCAAGCTATTTGTTTTTGGCACTGTTTGC
TATTGCGGTCATTATTACCATGATGACCAGCATGATAGAGACGTTGCCCAAAGAAAAACGTAAACACGAGTCTGTCGCGC
GCAGCTACCACTACGTTTTATCCAACAAACGCTTCCAAGGTTATTTGCTTTGCTTAGTCGCAACCTTTGCCGGTGTGGCG
GTTTTTGAGGCAGCGGCGGGCGTGCTATTAGGCGGTGTGTTAGGGCTGCCTGCGACTATCGTCAGCATTCTATTTGTATT
GCCGATCCCGGGCTATCTGATTGGTGCGGGCTTATCCAGCTACATTGCTCAGCGTCGCTCACAAAAGCGTGCTCTGAACG
TTGGCTTGGTCGCAATACTGATCGGCGCAGCCGTGGTGCTGATTCCAGGTCTGTTTGGTCAAACAACGGCACTGACGTTG
GTCGGTGGTGCGACGATTTACTTCCTTGGCGCAGGCATATTGTTCCCAGCGGCGACCACGGGAGCACTGGCTCCTTTCCC
TTATCACGCAGGAACTGGTGGCGCTATTTTAGGCGGCATGCAAAATCTAGGAGCAGGTATCGCGACATTAATCGCCTCCT
TGTTCCCAGCTCAAGATCAACTGCCTCTTGGCTGTCTCATGCTCGCAATGTCTATCCTTGCGATGTTAGGGTTGCGTTTG
GTTTATCGTAAACCTGATTCTTCCAATGAAATGCCGCTGGCAATCTGA

Upstream 100 bases:

>100_bases
AGATCACATTTTCAGATTGATCACAATGCCATCAACGACTAATATCTCGCGCCGTAAACCCCTATAAAACTCATTATTTG
GCAGTCTTTACCGATCTGTC

Downstream 100 bases:

>100_bases
TCTTTCTAAGAGGCGATTTCATCGCCTCTTTTTTTATTTGCTGCGATAGCGATTATCCACCATCTGCAATGCGTTCAAGT
GCTTCAATTCTGCGCCACTG

Product: multidrug resistance protein D

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 415; Mature: 415

Protein sequence:

>415_residues
MVFFVFNLAGEFAQMSASFPLAKLTFLIAILTAVGQMTQTMYVPSIGHMASEFLVSPAALQAVMACYLIPYGLSQFVYGP
LSDRLGRKPIIIAGLLIYIAGTMLSLFAHEYTWFLVGSFVQGLGIGCGGAMSRTLTRDCFEGAELHRANSLISMCVIFSP
LMAPVLGGLLTETFGWRSSYLFLALFAIAVIITMMTSMIETLPKEKRKHESVARSYHYVLSNKRFQGYLLCLVATFAGVA
VFEAAAGVLLGGVLGLPATIVSILFVLPIPGYLIGAGLSSYIAQRRSQKRALNVGLVAILIGAAVVLIPGLFGQTTALTL
VGGATIYFLGAGILFPAATTGALAPFPYHAGTGGAILGGMQNLGAGIATLIASLFPAQDQLPLGCLMLAMSILAMLGLRL
VYRKPDSSNEMPLAI

Sequences:

>Translated_415_residues
MVFFVFNLAGEFAQMSASFPLAKLTFLIAILTAVGQMTQTMYVPSIGHMASEFLVSPAALQAVMACYLIPYGLSQFVYGP
LSDRLGRKPIIIAGLLIYIAGTMLSLFAHEYTWFLVGSFVQGLGIGCGGAMSRTLTRDCFEGAELHRANSLISMCVIFSP
LMAPVLGGLLTETFGWRSSYLFLALFAIAVIITMMTSMIETLPKEKRKHESVARSYHYVLSNKRFQGYLLCLVATFAGVA
VFEAAAGVLLGGVLGLPATIVSILFVLPIPGYLIGAGLSSYIAQRRSQKRALNVGLVAILIGAAVVLIPGLFGQTTALTL
VGGATIYFLGAGILFPAATTGALAPFPYHAGTGGAILGGMQNLGAGIATLIASLFPAQDQLPLGCLMLAMSILAMLGLRL
VYRKPDSSNEMPLAI
>Mature_415_residues
MVFFVFNLAGEFAQMSASFPLAKLTFLIAILTAVGQMTQTMYVPSIGHMASEFLVSPAALQAVMACYLIPYGLSQFVYGP
LSDRLGRKPIIIAGLLIYIAGTMLSLFAHEYTWFLVGSFVQGLGIGCGGAMSRTLTRDCFEGAELHRANSLISMCVIFSP
LMAPVLGGLLTETFGWRSSYLFLALFAIAVIITMMTSMIETLPKEKRKHESVARSYHYVLSNKRFQGYLLCLVATFAGVA
VFEAAAGVLLGGVLGLPATIVSILFVLPIPGYLIGAGLSSYIAQRRSQKRALNVGLVAILIGAAVVLIPGLFGQTTALTL
VGGATIYFLGAGILFPAATTGALAPFPYHAGTGGAILGGMQNLGAGIATLIASLFPAQDQLPLGCLMLAMSILAMLGLRL
VYRKPDSSNEMPLAI

Specific function: Multidrug resistance pump that participates in a low energy shock adaptative response [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily [H]

Homologues:

Organism=Escherichia coli, GI87082312, Length=346, Percent_Identity=42.485549132948, Blast_Score=266, Evalue=3e-72,
Organism=Escherichia coli, GI48994889, Length=342, Percent_Identity=23.6842105263158, Blast_Score=107, Evalue=1e-24,
Organism=Escherichia coli, GI1788509, Length=356, Percent_Identity=25.561797752809, Blast_Score=102, Evalue=6e-23,
Organism=Escherichia coli, GI1790146, Length=376, Percent_Identity=25.531914893617, Blast_Score=96, Evalue=6e-21,
Organism=Escherichia coli, GI1787065, Length=312, Percent_Identity=25.9615384615385, Blast_Score=82, Evalue=8e-17,
Organism=Escherichia coli, GI1790794, Length=320, Percent_Identity=20.625, Blast_Score=72, Evalue=7e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR004734 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 43889; Mature: 43889

Theoretical pI: Translated: 9.09; Mature: 9.09

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure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HHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8240355; 7686882; 9278503 [H]