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The map label for this gene is insE1 [H]

Identifier: 30065063

GI number: 30065063

Start: 3963698

End: 3963997

Strand: Direct

Name: insE1 [H]

Synonym: S4064

Alternate gene names: 30065063

Gene position: 3963698-3963997 (Clockwise)

Preceding gene: 30065061

Following gene: 30065064

Centisome position: 86.18

GC content: 52.67

Gene sequence:

>300_bases
ATGACAAAACCAGTATCAATCAGCAAGAAGCCCCGTAAACAACATACGCCTGAATTTCGTAACGAAGCCCTGAAACTCGC
TGAACGCATCGGTGTGGCCGCCGCAGCCCGTGAACTCAGCCTGTATGAATCTCAGCTTTATGCCTGGCGCAGTAAACAGC
AGCAACAAATGAGTTCGTCAGAGCGCGAAAGCGAACTGGCCGCTGAAAATGTCCGCCTTAAACGACAACTGGCGGAGCAG
GCTGAGGAACTGTCCATCCTCCAAAAGGCCGCGACATACTTCGCGAAGCGCCTGAAATGA

Upstream 100 bases:

>100_bases
TATATCAGAAAGCGCCCGTTTTAGTTTTAATGGAGGGTGATCTTACCCACCAATAGTGGACACAGGACTAAGTGAGTAAA
CTCTCAACCAGAGGTGACTC

Downstream 100 bases:

>100_bases
AGTATGTCTTTATCGAAAATCATCGGGCAGAGTTCAGCATCAAAGCGATGTGTCGTGTACTTCGGGTTGCCCGCAGCGGC
TGGTATGTCTGGCTCAGGCG

Product: IS3 orfA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 99; Mature: 98

Protein sequence:

>99_residues
MTKPVSISKKPRKQHTPEFRNEALKLAERIGVAAAARELSLYESQLYAWRSKQQQQMSSSERESELAAENVRLKRQLAEQ
AEELSILQKAATYFAKRLK

Sequences:

>Translated_99_residues
MTKPVSISKKPRKQHTPEFRNEALKLAERIGVAAAARELSLYESQLYAWRSKQQQQMSSSERESELAAENVRLKRQLAEQ
AEELSILQKAATYFAKRLK
>Mature_98_residues
TKPVSISKKPRKQHTPEFRNEALKLAERIGVAAAARELSLYESQLYAWRSKQQQQMSSSERESELAAENVRLKRQLAEQA
EELSILQKAATYFAKRLK

Specific function: Involved in the transposition of the insertion sequence IS3 [H]

COG id: COG2963

COG function: function code L; Transposase and inactivated derivatives

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transposase 8 family [H]

Homologues:

Organism=Escherichia coli, GI226510958, Length=99, Percent_Identity=81.8181818181818, Blast_Score=159, Evalue=5e-41,
Organism=Escherichia coli, GI226510933, Length=99, Percent_Identity=81.8181818181818, Blast_Score=159, Evalue=5e-41,
Organism=Escherichia coli, GI226510930, Length=99, Percent_Identity=81.8181818181818, Blast_Score=159, Evalue=5e-41,
Organism=Escherichia coli, GI226510928, Length=99, Percent_Identity=81.8181818181818, Blast_Score=159, Evalue=5e-41,
Organism=Escherichia coli, GI226510927, Length=99, Percent_Identity=81.8181818181818, Blast_Score=159, Evalue=5e-41,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002514 [H]

Pfam domain/function: PF01527 Transposase_8 [H]

EC number: NA

Molecular weight: Translated: 11405; Mature: 11274

Theoretical pI: Translated: 10.56; Mature: 10.56

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKPVSISKKPRKQHTPEFRNEALKLAERIGVAAAARELSLYESQLYAWRSKQQQQMSSS
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ERESELAAENVRLKRQLAEQAEELSILQKAATYFAKRLK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TKPVSISKKPRKQHTPEFRNEALKLAERIGVAAAARELSLYESQLYAWRSKQQQQMSSS
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ERESELAAENVRLKRQLAEQAEELSILQKAATYFAKRLK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2987844; 9278503 [H]