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The map label for this gene is insF1 [C]

Identifier: 30065061

GI number: 30065061

Start: 3962267

End: 3962914

Strand: Direct

Name: insF1 [C]

Synonym: S4063

Alternate gene names: 30065061

Gene position: 3962267-3962914 (Clockwise)

Preceding gene: 30065060

Following gene: 30065063

Centisome position: 86.15

GC content: 54.63

Gene sequence:

>648_bases
GTGGCCAGATGCACTGTGGCACGTCTCATGGCGGTTATGGGACTTGCCGGTGTTCTCCGGGGTAAAAAGGTCCGTACGAC
CATCAGCCGGAAAGCCGTTGCCGCAGGCGACCGCGTAAACCGTCAGTTCGTGGCAGAACGACCTGACCAGCTGTGGGTGG
CTGATTTTACTTACGTCAGCACATGGCGGGGCTTCGTCTATGTGGCGTTCATCATTGATGTGTTTGCCGGATACATCGTG
GGGTGGCGGGTCTCATCGTCCATGGAAACGACATTCGTGCTGGATGCACTGGAGCAGGCGTTATGGGCCCGTCGACCGTC
CGGCACGGTCCATCACAGTGATAAAGGTTCTCAGTATGTATCGCTGGTCTACACACAGCGGCTTAAGGAAGCCGGATTAC
TGGCATCAACAGGAAGTACAGGCGACTCGTATGACAACGCGATGGCGGAGAGCATCAATGGTCTTTACAAAGCGGAGGTA
ATACACCGTAAGAGCTGGAAAAACCGTGCAGAAGTGGAACTGGCCACACTAACGTGGGTGGACTGGTATAACAATCGACG
ATTGCTGGAAAGGCTGGGCCACATCCCTCCGGCAGAAGCAGAAAAAGCTTATTATGCTTCCATCGGAAACGATGATCTGG
CAGCCTGA

Upstream 100 bases:

>100_bases
GCGATGACTGGCTGAAGAAAGAGATACAGCGCGTATACGATGAAAATCACAAGGTATACGGTGTAAAGTCTGGCGTCAGT
TGTTACGGGAAGGTATCAGA

Downstream 100 bases:

>100_bases
GTTCACAGATAAAACACTCTCCAGGAAAACCGGGGCGGTTCAAGTAGATACATACGCAGTCAGAGTACACTTACTTTTTA
TTATTTCCTGCAATGGCGAT

Product: IS629 orfB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 215; Mature: 214

Protein sequence:

>215_residues
MARCTVARLMAVMGLAGVLRGKKVRTTISRKAVAAGDRVNRQFVAERPDQLWVADFTYVSTWRGFVYVAFIIDVFAGYIV
GWRVSSSMETTFVLDALEQALWARRPSGTVHHSDKGSQYVSLVYTQRLKEAGLLASTGSTGDSYDNAMAESINGLYKAEV
IHRKSWKNRAEVELATLTWVDWYNNRRLLERLGHIPPAEAEKAYYASIGNDDLAA

Sequences:

>Translated_215_residues
MARCTVARLMAVMGLAGVLRGKKVRTTISRKAVAAGDRVNRQFVAERPDQLWVADFTYVSTWRGFVYVAFIIDVFAGYIV
GWRVSSSMETTFVLDALEQALWARRPSGTVHHSDKGSQYVSLVYTQRLKEAGLLASTGSTGDSYDNAMAESINGLYKAEV
IHRKSWKNRAEVELATLTWVDWYNNRRLLERLGHIPPAEAEKAYYASIGNDDLAA
>Mature_214_residues
ARCTVARLMAVMGLAGVLRGKKVRTTISRKAVAAGDRVNRQFVAERPDQLWVADFTYVSTWRGFVYVAFIIDVFAGYIVG
WRVSSSMETTFVLDALEQALWARRPSGTVHHSDKGSQYVSLVYTQRLKEAGLLASTGSTGDSYDNAMAESINGLYKAEVI
HRKSWKNRAEVELATLTWVDWYNNRRLLERLGHIPPAEAEKAYYASIGNDDLAA

Specific function: Involved in the transposition of the insertion sequence [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 integrase catalytic domain [H]

Homologues:

Organism=Homo sapiens, GI310129419, Length=179, Percent_Identity=55.8659217877095, Blast_Score=192, Evalue=1e-49,
Organism=Escherichia coli, GI1788405, Length=202, Percent_Identity=32.1782178217822, Blast_Score=106, Evalue=1e-24,
Organism=Escherichia coli, GI1787263, Length=202, Percent_Identity=32.1782178217822, Blast_Score=106, Evalue=1e-24,
Organism=Escherichia coli, GI1786753, Length=202, Percent_Identity=32.1782178217822, Blast_Score=106, Evalue=1e-24,
Organism=Escherichia coli, GI1786570, Length=202, Percent_Identity=32.1782178217822, Blast_Score=106, Evalue=1e-24,
Organism=Escherichia coli, GI1786490, Length=202, Percent_Identity=32.1782178217822, Blast_Score=106, Evalue=1e-24,
Organism=Escherichia coli, GI1789981, Length=201, Percent_Identity=29.3532338308458, Blast_Score=100, Evalue=1e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001584
- InterPro:   IPR012337 [H]

Pfam domain/function: PF00665 rve [H]

EC number: NA

Molecular weight: Translated: 24043; Mature: 23911

Theoretical pI: Translated: 9.64; Mature: 9.64

Prosite motif: PS50994 INTEGRASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARCTVARLMAVMGLAGVLRGKKVRTTISRKAVAAGDRVNRQFVAERPDQLWVADFTYVS
CCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHHCCCCCEEEEHHHHHH
TWRGFVYVAFIIDVFAGYIVGWRVSSSMETTFVLDALEQALWARRPSGTVHHSDKGSQYV
HHHHHHHHHHHHHHHHHHHHHEEECCCCHHHHHHHHHHHHHHHCCCCCCEEECCCCCHHH
SLVYTQRLKEAGLLASTGSTGDSYDNAMAESINGLYKAEVIHRKSWKNRAEVELATLTWV
HHHHHHHHHHCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEE
DWYNNRRLLERLGHIPPAEAEKAYYASIGNDDLAA
HHHCCHHHHHHHCCCCCCCCCHHEEEECCCCCCCC
>Mature Secondary Structure 
ARCTVARLMAVMGLAGVLRGKKVRTTISRKAVAAGDRVNRQFVAERPDQLWVADFTYVS
CCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHHCCCCCEEEEHHHHHH
TWRGFVYVAFIIDVFAGYIVGWRVSSSMETTFVLDALEQALWARRPSGTVHHSDKGSQYV
HHHHHHHHHHHHHHHHHHHHHEEECCCCHHHHHHHHHHHHHHHCCCCCCEEECCCCCHHH
SLVYTQRLKEAGLLASTGSTGDSYDNAMAESINGLYKAEVIHRKSWKNRAEVELATLTWV
HHHHHHHHHHCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEE
DWYNNRRLLERLGHIPPAEAEKAYYASIGNDDLAA
HHHCCHHHHHHHCCCCCCCCCHHEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2159625; 2824781 [H]