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The map label for this gene is insF1 [H]

Identifier: 30065064

GI number: 30065064

Start: 3963994

End: 3964386

Strand: Direct

Name: insF1 [H]

Synonym: S4832

Alternate gene names: 30065064

Gene position: 3963994-3964386 (Clockwise)

Preceding gene: 30065063

Following gene: 30065068

Centisome position: 86.19

GC content: 58.02

Gene sequence:

>393_bases
ATGAAGTATGTCTTTATCGAAAATCATCGGGCAGAGTTCAGCATCAAAGCGATGTGTCGTGTACTTCGGGTTGCCCGCAG
CGGCTGGTATGTCTGGCTCAGGCGTCGTCACCAGATGAGCCTGCGCCAACAGTTTCGGCTCACCTGCGATGCCGCTGTCC
ATAAGGCATTCTTTGAGGCAAAGCAGCGATACGGTGCTCCCCGCCTTGCTGACGAACTGCCGGAGTTCAATATTAAAACC
ATTGCCGCCAGCCTGCGTCGTCAGGGGCTGCGGGCGAAAGCCAGCCGGAAGTTCAGCCCGGTCAGCTACCGTGCACATGG
CCTGCCCGTATTGGAGAATCTGCTGGAGCAGGACTTCAGCGCCAGTGGCCCGAAACCAGAAGTGGGCGGGTGA

Upstream 100 bases:

>100_bases
TGGCCGCTGAAAATGTCCGCCTTAAACGACAACTGGCGGAGCAGGCTGAGGAACTGTCCATCCTCCAAAAGGCCGCGACA
TACTTCGCGAAGCGCCTGAA

Downstream 100 bases:

>100_bases
CATCACGTACTTGCGTACCGATGAGGGCTGGTTGTATCTCGCAGTAGTCATCGACCTGTGGTCACGCGCCGTCATTGGCT
GGTCGATGTCACCGCGAATG

Product: IS3 orfB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 130; Mature: 130

Protein sequence:

>130_residues
MKYVFIENHRAEFSIKAMCRVLRVARSGWYVWLRRRHQMSLRQQFRLTCDAAVHKAFFEAKQRYGAPRLADELPEFNIKT
IAASLRRQGLRAKASRKFSPVSYRAHGLPVLENLLEQDFSASGPKPEVGG

Sequences:

>Translated_130_residues
MKYVFIENHRAEFSIKAMCRVLRVARSGWYVWLRRRHQMSLRQQFRLTCDAAVHKAFFEAKQRYGAPRLADELPEFNIKT
IAASLRRQGLRAKASRKFSPVSYRAHGLPVLENLLEQDFSASGPKPEVGG
>Mature_130_residues
MKYVFIENHRAEFSIKAMCRVLRVARSGWYVWLRRRHQMSLRQQFRLTCDAAVHKAFFEAKQRYGAPRLADELPEFNIKT
IAASLRRQGLRAKASRKFSPVSYRAHGLPVLENLLEQDFSASGPKPEVGG

Specific function: Involved in the transposition of the insertion sequence IS3 [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 integrase catalytic domain [H]

Homologues:

Organism=Escherichia coli, GI1788405, Length=134, Percent_Identity=74.6268656716418, Blast_Score=200, Evalue=3e-53,
Organism=Escherichia coli, GI1787263, Length=134, Percent_Identity=74.6268656716418, Blast_Score=200, Evalue=3e-53,
Organism=Escherichia coli, GI1786753, Length=134, Percent_Identity=74.6268656716418, Blast_Score=200, Evalue=3e-53,
Organism=Escherichia coli, GI1786570, Length=134, Percent_Identity=74.6268656716418, Blast_Score=200, Evalue=3e-53,
Organism=Escherichia coli, GI1786490, Length=134, Percent_Identity=74.6268656716418, Blast_Score=200, Evalue=3e-53,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001584
- InterPro:   IPR012337 [H]

Pfam domain/function: PF00665 rve [H]

EC number: NA

Molecular weight: Translated: 15021; Mature: 15021

Theoretical pI: Translated: 11.17; Mature: 11.17

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKYVFIENHRAEFSIKAMCRVLRVARSGWYVWLRRRHQMSLRQQFRLTCDAAVHKAFFEA
CEEEEEECCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KQRYGAPRLADELPEFNIKTIAASLRRQGLRAKASRKFSPVSYRAHGLPVLENLLEQDFS
HHHCCCCCHHHHCCCCCHHHHHHHHHHHCHHHHHHCCCCCCCCCCCCCHHHHHHHHHHCC
ASGPKPEVGG
CCCCCCCCCC
>Mature Secondary Structure
MKYVFIENHRAEFSIKAMCRVLRVARSGWYVWLRRRHQMSLRQQFRLTCDAAVHKAFFEA
CEEEEEECCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KQRYGAPRLADELPEFNIKTIAASLRRQGLRAKASRKFSPVSYRAHGLPVLENLLEQDFS
HHHCCCCCHHHHCCCCCHHHHHHHHHHHCHHHHHHCCCCCCCCCCCCCHHHHHHHHHHCC
ASGPKPEVGG
CCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2987844; 9278503 [H]