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The map label for this gene is ygjN

Identifier: 30064426

GI number: 30064426

Start: 3213042

End: 3213356

Strand: Reverse

Name: ygjN

Synonym: S3330

Alternate gene names: 30064426

Gene position: 3213356-3213042 (Counterclockwise)

Preceding gene: 30064427

Following gene: 30064425

Centisome position: 69.87

GC content: 38.41

Gene sequence:

>315_bases
ATGCACCTGATAACTCAAAAAGCATTGAAAGATGCTGCGGAAAAATACCCGCAACATAAAACGGAGTTGGTGGCTCTGGG
GAACACGATTGCTAAGGGATATTTCAAAAAACCTGAGTCATTAAAAGCAGTATTCCCATCTCTGGATAACTTCAAATATC
TGGATAAGCATTATGTTTTCAATGTTGGGGGCAATGAATTACGTGTTGTAGCAATGGTCTTTTTTGAATCGCAAAAGTGC
TACATACGTGAAGTTATGACGCATAAAGAATACGATTTCTTTACCGCTGTTCATCGTACTAAGGGGAAAAAATGA

Upstream 100 bases:

>100_bases
TTTCTACGTTTATTCTTCCGTCACACAGATAAATTCATCCGTTGCCAATCTGTCAACGGATGTTATCATATTGCCAATTT
GTCAACGGACGTGATGAAGA

Downstream 100 bases:

>100_bases
TTGCGATTGCCGACATCTTGCAAGCAGGAGAAAAGCTAACTGCTGTGGCACCTTTTCTGGCGGGTATTCAGAACGAGGAA
CAATACACCCAGGCGCTGGA

Product: hypothetical protein

Products: NA

Alternate protein names: Endoribonuclease HigB; Toxin HigB

Number of amino acids: Translated: 104; Mature: 104

Protein sequence:

>104_residues
MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAVFPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKC
YIREVMTHKEYDFFTAVHRTKGKK

Sequences:

>Translated_104_residues
MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAVFPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKC
YIREVMTHKEYDFFTAVHRTKGKK
>Mature_104_residues
MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAVFPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKC
YIREVMTHKEYDFFTAVHRTKGKK

Specific function: Part of a toxin-antitoxin (TA) module. A probable translation-dependent mRNA interferase

COG id: COG4680

COG function: function code S; Uncharacterized protein conserved in bacteria

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HigB mRNA interferase family

Homologues:

Organism=Escherichia coli, GI1789465, Length=104, Percent_Identity=100, Blast_Score=216, Evalue=3e-58,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HIGB_ECO57 (P64579)

Other databases:

- EMBL:   AE005174
- EMBL:   BA000007
- PIR:   D85969
- PIR:   E91124
- RefSeq:   NP_289657.1
- RefSeq:   NP_311992.1
- ProteinModelPortal:   P64579
- EnsemblBacteria:   EBESCT00000024115
- EnsemblBacteria:   EBESCT00000057596
- GeneID:   916202
- GeneID:   958558
- GenomeReviews:   AE005174_GR
- GenomeReviews:   BA000007_GR
- KEGG:   ece:Z4436
- KEGG:   ecs:ECs3965
- GeneTree:   EBGT00050000010506
- HOGENOM:   HBG391572
- OMA:   LTHDEYD
- ProtClustDB:   CLSK880573
- BioCyc:   ECOL83334:ECS3965-MONOMER
- InterPro:   IPR018669

Pfam domain/function: PF09907 DUF2136

EC number: NA

Molecular weight: Translated: 12103; Mature: 12103

Theoretical pI: Translated: 10.04; Mature: 10.04

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAVFPSLDNFKYLDKHYVF
CCCHHHHHHHHHHHHCCCCHHHHHHHCCHHHHHHCCCCHHHHHHCCCCCCCEECCCEEEE
NVGGNELRVVAMVFFESQKCYIREVMTHKEYDFFTAVHRTKGKK
ECCCCCEEEEEEEECCCCHHHHHHHHHCCCHHHHHHHHHHCCCC
>Mature Secondary Structure
MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAVFPSLDNFKYLDKHYVF
CCCHHHHHHHHHHHHCCCCHHHHHHHCCHHHHHHCCCCHHHHHHCCCCCCCEECCCEEEE
NVGGNELRVVAMVFFESQKCYIREVMTHKEYDFFTAVHRTKGKK
ECCCCCEEEEEEEECCCCHHHHHHHHHCCCHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796