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The map label for this gene is ygjO

Identifier: 30064427

GI number: 30064427

Start: 3213641

End: 3214777

Strand: Reverse

Name: ygjO

Synonym: S3331

Alternate gene names: 30064427

Gene position: 3214777-3213641 (Counterclockwise)

Preceding gene: 30064433

Following gene: 30064426

Centisome position: 69.9

GC content: 53.56

Gene sequence:

>1137_bases
ATGAGCCACTTAGACAACGGTTTCCGTTCACTGACACTACAACGTTTTCCGGCGACGGATGACGTTAACCCGCTACAGGC
GTGGGAAGCGGCAGATGAATATTTGCTGCAACAGTTGGACGACACAGAAATCCTCGGCCCGGTGTTGATCCTGAATGATG
CCTTTGGTGCGTTAAGCTGCGCGCTGGCGGAACATAAGCCGTACAGCATTGGCGACTCATACATCAGTGAACTGGCGACG
CGCGAGAATTTACGCCTAAACGGGATTGATGAGTCGAGCGTGAAGTTTCTCGACAGCACCGCCGACTACCCGCAACAGCC
GGGTGTGGTGCTGATCAAAGTGCCGAAAACACTGGCATTGCTGGAACAGCAACTGCGTGCGCTGCGCAAAGTGGTCACGT
CGGATACACGTATTATTGCCGGTGCCAAAGCCCGTGACATTCACACTTCCACGCTGGAACTGTTCGAAAAAGTGCTCGGC
CCGACCACCACCACGCTGGCATGGAAAAAAGCGCGCCTGATTAATTGCACGTTCAATGAACCGCCGCTGGCCGATGCGCC
GCAGACCGTTAGCTGGAAGCTGGAAGGTACTGACTGGACTATCCATAACCATGCGAATGTCTTCTCCCGCACCGGGCTTG
ATATCGGCGCGCGCTTCTTTATGCAACATCTGCCAGAGAATCTCGAAGGGGAGATTGTCGATCTCGGTTGCGGTAATGGC
GTTATTGGTCTGACGCTGCTTGATAAAAACCCGCAGGCGAAAGTGGTGTTTGTCGATGAATCGCCGATGGCGGTTGCTTC
CAGCCGTTTGAACGTTGAAACCAACATGCCAGAGGCGTTGGATCGCAGCGAGTTTATGATCAACAACGCGCTCTCCGGCG
TGGAGCCTTTCCGCTTTAATGCTGTGCTCTGCAACCCTCCGTTTCACCAACAACATGCGCTGACCGATAACGTCGCCTGG
GAGATGTTCCACTACGCCCGCCGCTGCCTGAAAATCAACGGCGAGCTGTATATCGTTGCCAACCGCCATCTGGATTACTT
CCATAAACTGAAGAAAATTTTCGGCAACTGCACCACTATTGCGACGAATAATAAATTTGTGGTGCTGAAAGCGGTGAAGC
TGGGACGTCGTCGGTAA

Upstream 100 bases:

>100_bases
AAGTAAGATTGCTCATAACCGCCCACGAAAAAGAGAAAAAGGGTATACTCACGCACCCTTTTCAGGGGCAACGTCGAAAT
TTTATCATTCAGGAGGGCCG

Downstream 100 bases:

>100_bases
GATTGGGAGTTACCTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGTTCAGCACGTTACCCCCAGGCCGGATAAGA
CGCGGCAAGCGTCGCATCCG

Product: putative enzyme

Products: NA

Alternate protein names: 23S rRNA m2G1835 methyltransferase; rRNA (guanine-N(2)-)-methyltransferase rlmG [H]

Number of amino acids: Translated: 378; Mature: 377

Protein sequence:

>378_residues
MSHLDNGFRSLTLQRFPATDDVNPLQAWEAADEYLLQQLDDTEILGPVLILNDAFGALSCALAEHKPYSIGDSYISELAT
RENLRLNGIDESSVKFLDSTADYPQQPGVVLIKVPKTLALLEQQLRALRKVVTSDTRIIAGAKARDIHTSTLELFEKVLG
PTTTTLAWKKARLINCTFNEPPLADAPQTVSWKLEGTDWTIHNHANVFSRTGLDIGARFFMQHLPENLEGEIVDLGCGNG
VIGLTLLDKNPQAKVVFVDESPMAVASSRLNVETNMPEALDRSEFMINNALSGVEPFRFNAVLCNPPFHQQHALTDNVAW
EMFHYARRCLKINGELYIVANRHLDYFHKLKKIFGNCTTIATNNKFVVLKAVKLGRRR

Sequences:

>Translated_378_residues
MSHLDNGFRSLTLQRFPATDDVNPLQAWEAADEYLLQQLDDTEILGPVLILNDAFGALSCALAEHKPYSIGDSYISELAT
RENLRLNGIDESSVKFLDSTADYPQQPGVVLIKVPKTLALLEQQLRALRKVVTSDTRIIAGAKARDIHTSTLELFEKVLG
PTTTTLAWKKARLINCTFNEPPLADAPQTVSWKLEGTDWTIHNHANVFSRTGLDIGARFFMQHLPENLEGEIVDLGCGNG
VIGLTLLDKNPQAKVVFVDESPMAVASSRLNVETNMPEALDRSEFMINNALSGVEPFRFNAVLCNPPFHQQHALTDNVAW
EMFHYARRCLKINGELYIVANRHLDYFHKLKKIFGNCTTIATNNKFVVLKAVKLGRRR
>Mature_377_residues
SHLDNGFRSLTLQRFPATDDVNPLQAWEAADEYLLQQLDDTEILGPVLILNDAFGALSCALAEHKPYSIGDSYISELATR
ENLRLNGIDESSVKFLDSTADYPQQPGVVLIKVPKTLALLEQQLRALRKVVTSDTRIIAGAKARDIHTSTLELFEKVLGP
TTTTLAWKKARLINCTFNEPPLADAPQTVSWKLEGTDWTIHNHANVFSRTGLDIGARFFMQHLPENLEGEIVDLGCGNGV
IGLTLLDKNPQAKVVFVDESPMAVASSRLNVETNMPEALDRSEFMINNALSGVEPFRFNAVLCNPPFHQQHALTDNVAWE
MFHYARRCLKINGELYIVANRHLDYFHKLKKIFGNCTTIATNNKFVVLKAVKLGRRR

Specific function: Specifically methylates the guanosine in position 1835 (m2G1835) of 23S rRNA [H]

COG id: COG2813

COG function: function code J; 16S RNA G1207 methylase RsmC

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RlmG family [H]

Homologues:

Organism=Escherichia coli, GI87082206, Length=378, Percent_Identity=98.9417989417989, Blast_Score=775, Evalue=0.0,
Organism=Escherichia coli, GI1790830, Length=173, Percent_Identity=33.5260115606936, Blast_Score=100, Evalue=2e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002052
- InterPro:   IPR017237
- InterPro:   IPR007848 [H]

Pfam domain/function: PF05175 MTS [H]

EC number: =2.1.1.174 [H]

Molecular weight: Translated: 42268; Mature: 42137

Theoretical pI: Translated: 6.58; Mature: 6.58

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSHLDNGFRSLTLQRFPATDDVNPLQAWEAADEYLLQQLDDTEILGPVLILNDAFGALSC
CCCCCCCHHHEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCHHHCCEEEEECCHHHHHH
ALAEHKPYSIGDSYISELATRENLRLNGIDESSVKFLDSTADYPQQPGVVLIKVPKTLAL
HHHCCCCCCCCHHHHHHHHHHCCCEECCCCHHHHHHHHCCCCCCCCCCEEEEECCHHHHH
LEQQLRALRKVVTSDTRIIAGAKARDIHTSTLELFEKVLGPTTTTLAWKKARLINCTFNE
HHHHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHHHHHHCCCEEEEEEEEEEEEEEECCC
PPLADAPQTVSWKLEGTDWTIHNHANVFSRTGLDIGARFFMQHLPENLEGEIVDLGCGNG
CCCCCCCCCEEEEECCCEEEEECCCCHHHHCCCHHHHHHHHHHCCCCCCCCEEEEECCCC
VIGLTLLDKNPQAKVVFVDESPMAVASSRLNVETNMPEALDRSEFMINNALSGVEPFRFN
EEEEEEECCCCCEEEEEECCCCHHHHHCCCEEECCCCHHHCHHHHHHHHHHCCCCCEEEE
AVLCNPPFHQQHALTDNVAWEMFHYARRCLKINGELYIVANRHLDYFHKLKKIFGNCTTI
EEEECCCCCCCCCCHHHHHHHHHHHHHHHEEECCEEEEEECCCHHHHHHHHHHHCCCEEE
ATNNKFVVLKAVKLGRRR
EECCCEEEEEEECCCCCC
>Mature Secondary Structure 
SHLDNGFRSLTLQRFPATDDVNPLQAWEAADEYLLQQLDDTEILGPVLILNDAFGALSC
CCCCCCHHHEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCHHHCCEEEEECCHHHHHH
ALAEHKPYSIGDSYISELATRENLRLNGIDESSVKFLDSTADYPQQPGVVLIKVPKTLAL
HHHCCCCCCCCHHHHHHHHHHCCCEECCCCHHHHHHHHCCCCCCCCCCEEEEECCHHHHH
LEQQLRALRKVVTSDTRIIAGAKARDIHTSTLELFEKVLGPTTTTLAWKKARLINCTFNE
HHHHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHHHHHHCCCEEEEEEEEEEEEEEECCC
PPLADAPQTVSWKLEGTDWTIHNHANVFSRTGLDIGARFFMQHLPENLEGEIVDLGCGNG
CCCCCCCCCEEEEECCCEEEEECCCCHHHHCCCHHHHHHHHHHCCCCCCCCEEEEECCCC
VIGLTLLDKNPQAKVVFVDESPMAVASSRLNVETNMPEALDRSEFMINNALSGVEPFRFN
EEEEEEECCCCCEEEEEECCCCHHHHHCCCEEECCCCHHHCHHHHHHHHHHCCCCCEEEE
AVLCNPPFHQQHALTDNVAWEMFHYARRCLKINGELYIVANRHLDYFHKLKKIFGNCTTI
EEEECCCCCCCCCCHHHHHHHHHHHHHHHEEECCEEEEEECCCHHHHHHHHHHHCCCEEE
ATNNKFVVLKAVKLGRRR
EECCCEEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA