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The map label for this gene is ygjM

Identifier: 30064425

GI number: 30064425

Start: 3212629

End: 3213045

Strand: Reverse

Name: ygjM

Synonym: S3329

Alternate gene names: 30064425

Gene position: 3213045-3212629 (Counterclockwise)

Preceding gene: 30064426

Following gene: 30064412

Centisome position: 69.86

GC content: 48.44

Gene sequence:

>417_bases
ATGATTGCGATTGCCGACATCTTGCAAGCAGGAGAAAAGCTAACTGCTGTGGCACCTTTTCTGGCGGGTATTCAGAACGA
GGAACAATACACCCAGGCGCTGGAACTGGTAGATCATCTGCTGCTCAACGATCCTGAAAACCCCTTGCTGGATCTGGTGT
GTGCCAAAATAACCGCGTGGGAAGAATCAGCGCCCGAATTTGCGGAATTTAATGCCATGGCTCAAGCCATGCCTGGCGGT
ATAGCCGTGATTCGTACCCTTATGGATCAATATGGTTTAACCCTTTCCGATCTGCCGGAAATTGGCAGTAAATCTATGGT
GTCACGCGTTTTGAGCGGGAAGAGGAAATTAACGCTGGAACACGCTAAAAAATTGGCAACGCGATTCGGCATTTCTCCCG
CCTTGTTTATTGATTAA

Upstream 100 bases:

>100_bases
CAATGGTCTTTTTTGAATCGCAAAAGTGCTACATACGTGAAGTTATGACGCATAAAGAATACGATTTCTTTACCGCTGTT
CATCGTACTAAGGGGAAAAA

Downstream 100 bases:

>100_bases
CGCGACGTGCCGAATGCGTTGCATCCGGCGCTTCAAATAACGATTTAAATCTCCAGCGCCAGCCGTGTTCCCTGGGCAAT
TGCCCGTCGTGCGTCCAGCT

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 138; Mature: 138

Protein sequence:

>138_residues
MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLNDPENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGG
IAVIRTLMDQYGLTLSDLPEIGSKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID

Sequences:

>Translated_138_residues
MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLNDPENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGG
IAVIRTLMDQYGLTLSDLPEIGSKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID
>Mature_138_residues
MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLNDPENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGG
IAVIRTLMDQYGLTLSDLPEIGSKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID

Specific function: Part of a toxin-antitoxin (TA) module. Functions as an mRNA interferase antitoxin

COG id: COG5499

COG function: function code K; Predicted transcription regulator containing HTH domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH cro/C1-type DNA-binding domain

Homologues:

Organism=Escherichia coli, GI1789464, Length=138, Percent_Identity=100, Blast_Score=281, Evalue=1e-77,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HIGA_ECOL6 (P67702)

Other databases:

- EMBL:   AE014075
- RefSeq:   NP_755711.1
- ProteinModelPortal:   P67702
- SMR:   P67702
- EnsemblBacteria:   EBESCT00000043026
- GeneID:   1040003
- GenomeReviews:   AE014075_GR
- KEGG:   ecc:c3840
- GeneTree:   EBGT00050000011376
- HOGENOM:   HBG391571
- OMA:   LACAKIT
- ProtClustDB:   CLSK880572
- GO:   GO:0006350
- InterPro:   IPR001387
- InterPro:   IPR010982
- SMART:   SM00530

Pfam domain/function: PF01381 HTH_3; SSF47413 Lambda_like_DNA

EC number: NA

Molecular weight: Translated: 14996; Mature: 14996

Theoretical pI: Translated: 4.45; Mature: 4.45

Prosite motif: PS50943 HTH_CROC1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLNDPENPLLDLVCAKITAW
CCHHHHHHHCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
EESAPEFAEFNAMAQAMPGGIAVIRTLMDQYGLTLSDLPEIGSKSMVSRVLSGKRKLTLE
HCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHCCCHHHHHHHHHCCCHHHHHH
HAKKLATRFGISPALFID
HHHHHHHHHCCCCCEECC
>Mature Secondary Structure
MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLNDPENPLLDLVCAKITAW
CCHHHHHHHCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
EESAPEFAEFNAMAQAMPGGIAVIRTLMDQYGLTLSDLPEIGSKSMVSRVLSGKRKLTLE
HCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHCCCHHHHHHHHHCCCHHHHHH
HAKKLATRFGISPALFID
HHHHHHHHHCCCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157