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The map label for this gene is insE1

Identifier: 30064021

GI number: 30064021

Start: 2751589

End: 2751888

Strand: Direct

Name: insE1

Synonym: S2861

Alternate gene names: 30064021

Gene position: 2751589-2751888 (Clockwise)

Preceding gene: 30064020

Following gene: 30064022

Centisome position: 59.83

GC content: 50.33

Gene sequence:

>300_bases
ATGACAAAAACAGTATCAACCAGTAAAAAACCCCGTAAACAGCATTCGCCTGAATTTCGCAGTGAAGCCCTGAAGCTTGC
TGAACGCATCGGTGTTACTGCCGCAGCCCGTGAACTCAGCCTGTATGAATCACAGCTCTACAACTGGCGCAGTAAACAGC
AAAATCAGCAGACGTCTTCTGAACGTGAACTGGAGATGTCTACCGAGATTGCACGTCTCAAACGCCAGCTGGCAGAACGG
GATGAAGAGCTGGCTATCCTCCAAAAGGCCGCGACATACTTCGCGAAGCGCCTGAAATGA

Upstream 100 bases:

>100_bases
ATTCAGCGGCTTCATTGAGATCATCCAACGAATTTTGATCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAACT
CTCAGTCAGAGGTGACTCAC

Downstream 100 bases:

>100_bases
AGTATGTCTTTATTGAAAAACATCAGGCTGAGTTCAGCATCAAAGCAATGTGCCGCGTGCTCCGGGTGGCCCGCAGCGGC
TGGTATACGTGGTGTCAGCG

Product: IS3 orfA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 99; Mature: 98

Protein sequence:

>99_residues
MTKTVSTSKKPRKQHSPEFRSEALKLAERIGVTAAARELSLYESQLYNWRSKQQNQQTSSERELEMSTEIARLKRQLAER
DEELAILQKAATYFAKRLK

Sequences:

>Translated_99_residues
MTKTVSTSKKPRKQHSPEFRSEALKLAERIGVTAAARELSLYESQLYNWRSKQQNQQTSSERELEMSTEIARLKRQLAER
DEELAILQKAATYFAKRLK
>Mature_98_residues
TKTVSTSKKPRKQHSPEFRSEALKLAERIGVTAAARELSLYESQLYNWRSKQQNQQTSSERELEMSTEIARLKRQLAERD
EELAILQKAATYFAKRLK

Specific function: Involved in the transposition of the insertion sequence IS3

COG id: COG2963

COG function: function code L; Transposase and inactivated derivatives

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transposase 8 family

Homologues:

Organism=Escherichia coli, GI226510958, Length=99, Percent_Identity=100, Blast_Score=196, Evalue=3e-52,
Organism=Escherichia coli, GI226510933, Length=99, Percent_Identity=100, Blast_Score=196, Evalue=3e-52,
Organism=Escherichia coli, GI226510930, Length=99, Percent_Identity=100, Blast_Score=196, Evalue=3e-52,
Organism=Escherichia coli, GI226510928, Length=99, Percent_Identity=100, Blast_Score=196, Evalue=3e-52,
Organism=Escherichia coli, GI226510927, Length=99, Percent_Identity=100, Blast_Score=196, Evalue=3e-52,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): INSE1_ECOLI (P0CF66)

Other databases:

- EMBL:   X02311
- EMBL:   U73857
- EMBL:   U00096
- EMBL:   AP009048
- PIR:   A64845
- RefSeq:   AP_000953.1
- RefSeq:   AP_001024.1
- RefSeq:   AP_001185.1
- RefSeq:   AP_001656.1
- RefSeq:   AP_002685.1
- RefSeq:   NP_414832.2
- RefSeq:   NP_414907.2
- RefSeq:   NP_415072.2
- RefSeq:   NP_415546.2
- RefSeq:   NP_416592.2
- ProteinModelPortal:   P0CF66
- SMR:   P0CF66
- EnsemblBacteria:   EBESCT00000000389
- EnsemblBacteria:   EBESCT00000015472
- GeneID:   944952
- GeneID:   945584
- GeneID:   945759
- GeneID:   946616
- GeneID:   946900
- GenomeReviews:   AP009048_GR
- GenomeReviews:   U00096_GR
- KEGG:   ecj:JW5036
- KEGG:   eco:b0298
- EchoBASE:   EB4709
- EcoGene:   EG40005
- eggNOG:   COG2963
- GeneTree:   EBGT00050000008545
- HOGENOM:   HBG756815
- InterPro:   IPR002514

Pfam domain/function: PF01527 Transposase_8

EC number: NA

Molecular weight: Translated: 11543; Mature: 11412

Theoretical pI: Translated: 10.56; Mature: 10.56

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKTVSTSKKPRKQHSPEFRSEALKLAERIGVTAAARELSLYESQLYNWRSKQQNQQTSS
CCCCCCCCCCCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ERELEMSTEIARLKRQLAERDEELAILQKAATYFAKRLK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TKTVSTSKKPRKQHSPEFRSEALKLAERIGVTAAARELSLYESQLYNWRSKQQNQQTSS
CCCCCCCCCCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ERELEMSTEIARLKRQLAERDEELAILQKAATYFAKRLK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2987844; 9278503