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The map label for this gene is insF1 [H]

Identifier: 30064022

GI number: 30064022

Start: 2751885

End: 2752313

Strand: Direct

Name: insF1 [H]

Synonym: S2862

Alternate gene names: 30064022

Gene position: 2751885-2752313 (Clockwise)

Preceding gene: 30064021

Following gene: 30064023

Centisome position: 59.83

GC content: 57.11

Gene sequence:

>429_bases
ATGAAGTATGTCTTTATTGAAAAACATCAGGCTGAGTTCAGCATCAAAGCAATGTGCCGCGTGCTCCGGGTGGCCCGCAG
CGGCTGGTATACGTGGTGTCAGCGGCGGACAAGGATAAGCACGCGTCAGCAGTTCCGCCAACACTGCGACAGCGTTGTCC
TCGCGGCTTTTACCCGGTCAAAACAGCGTTACGGTGCCCCACGCCTGACGGATGAACTGCGTGCTCAGGGTTACCCCTTT
AACGTAAAAACCGTGGCGGCAAGCCTGCGCCGTCAGGGACTGAGGGCAAAGGCCTCCCGGAAGTTCAGCCCGGTCAGCTA
CCGCGCACACGGCCTGCCTGTGTCAGAAAATCTGTTGGAGCAGGATTTTTACTGCCAGTGGCCCGAACCAGAAGTGGCCA
GGAGACATCACGTACTTACGTACAGATGA

Upstream 100 bases:

>100_bases
TGTCTACCGAGATTGCACGTCTCAAACGCCAGCTGGCAGAACGGGATGAAGAGCTGGCTATCCTCCAAAAGGCCGCGACA
TACTTCGCGAAGCGCCTGAA

Downstream 100 bases:

>100_bases
AGGCTGGCTGTATCTGGCAGTGGTCATTGACCTGTGGTCACGTGCCGTTATTGGCTGGTCAATGTCGCCACGCATGACGG
CGCAACCTGCCCTGCGATGC

Product: IS3 orfB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 142; Mature: 142

Protein sequence:

>142_residues
MKYVFIEKHQAEFSIKAMCRVLRVARSGWYTWCQRRTRISTRQQFRQHCDSVVLAAFTRSKQRYGAPRLTDELRAQGYPF
NVKTVAASLRRQGLRAKASRKFSPVSYRAHGLPVSENLLEQDFYCQWPEPEVARRHHVLTYR

Sequences:

>Translated_142_residues
MKYVFIEKHQAEFSIKAMCRVLRVARSGWYTWCQRRTRISTRQQFRQHCDSVVLAAFTRSKQRYGAPRLTDELRAQGYPF
NVKTVAASLRRQGLRAKASRKFSPVSYRAHGLPVSENLLEQDFYCQWPEPEVARRHHVLTYR
>Mature_142_residues
MKYVFIEKHQAEFSIKAMCRVLRVARSGWYTWCQRRTRISTRQQFRQHCDSVVLAAFTRSKQRYGAPRLTDELRAQGYPF
NVKTVAASLRRQGLRAKASRKFSPVSYRAHGLPVSENLLEQDFYCQWPEPEVARRHHVLTYR

Specific function: Involved in the transposition of the insertion sequence IS3 [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 integrase catalytic domain [H]

Homologues:

Organism=Escherichia coli, GI1788405, Length=133, Percent_Identity=94.7368421052632, Blast_Score=256, Evalue=3e-70,
Organism=Escherichia coli, GI1787263, Length=133, Percent_Identity=94.7368421052632, Blast_Score=256, Evalue=3e-70,
Organism=Escherichia coli, GI1786753, Length=133, Percent_Identity=94.7368421052632, Blast_Score=256, Evalue=3e-70,
Organism=Escherichia coli, GI1786570, Length=133, Percent_Identity=94.7368421052632, Blast_Score=256, Evalue=3e-70,
Organism=Escherichia coli, GI1786490, Length=133, Percent_Identity=94.7368421052632, Blast_Score=256, Evalue=3e-70,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001584
- InterPro:   IPR012337 [H]

Pfam domain/function: PF00665 rve [H]

EC number: NA

Molecular weight: Translated: 16822; Mature: 16822

Theoretical pI: Translated: 11.13; Mature: 11.13

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKYVFIEKHQAEFSIKAMCRVLRVARSGWYTWCQRRTRISTRQQFRQHCDSVVLAAFTRS
CCEEEEECCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KQRYGAPRLTDELRAQGYPFNVKTVAASLRRQGLRAKASRKFSPVSYRAHGLPVSENLLE
HHHCCCCCCCHHHHHCCCCCCHHHHHHHHHHHCHHHHHHCCCCCCCEECCCCCCHHHHHC
QDFYCQWPEPEVARRHHVLTYR
CCCCCCCCCHHHHHHCCCCCCC
>Mature Secondary Structure
MKYVFIEKHQAEFSIKAMCRVLRVARSGWYTWCQRRTRISTRQQFRQHCDSVVLAAFTRS
CCEEEEECCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KQRYGAPRLTDELRAQGYPFNVKTVAASLRRQGLRAKASRKFSPVSYRAHGLPVSENLLE
HHHCCCCCCCHHHHHCCCCCCHHHHHHHHHHHCHHHHHHCCCCCCCEECCCCCCHHHHHC
QDFYCQWPEPEVARRHHVLTYR
CCCCCCCCCHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2987844; 9278503 [H]