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The map label for this gene is insA [H]

Identifier: 30064020

GI number: 30064020

Start: 2750774

End: 2751073

Strand: Direct

Name: insA [H]

Synonym: S2860

Alternate gene names: 30064020

Gene position: 2750774-2751073 (Clockwise)

Preceding gene: 30064019

Following gene: 30064021

Centisome position: 59.81

GC content: 53.0

Gene sequence:

>300_bases
GTGGCTTCCATTTCCATCAGATGTCCTTCCTGCTCCGCTACTGAAGGCGTGGTGCGTAACGGCAAAAGCACTGCCGGACA
TCAGCGCTATCTCTGCTCTCATTGCCGTAAAACATGGCAACTACAGTTCACTTACACCGCCTCTCAGCCCGGTACGCACC
AGAAAATCATTGATATGGCCATGAATGGCGTCGGATATCGCGCCAGTGCACGCATTATGGGCGTTGGCCTCAACACGGTT
TTACGTCACTTAAAAAACTCAGGCCGCAGTGGTAACCTCGCGCATACAACCAGGCAGTGA

Upstream 100 bases:

>100_bases
TTTAGTAGTAATATTAGGATTGGTGATTCTGCACTCCAGAAGTTCGGTAATGCTGCCAACTTACTAATTTAGTGTATGAT
GGTGATTTTAAGGTGCTTGC

Downstream 100 bases:

>100_bases
TGTGATTGTCTGCGCTGAAATGGACGAACATTGGGGCTACGTCGGTGCTAAATCACGTCAGCGCTGGCTGTTTTACGCGT
ATGACAGGATACGGAGGACG

Product: IS1 orfA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 99; Mature: 98

Protein sequence:

>99_residues
MASISIRCPSCSATEGVVRNGKSTAGHQRYLCSHCRKTWQLQFTYTASQPGTHQKIIDMAMNGVGYRASARIMGVGLNTV
LRHLKNSGRSGNLAHTTRQ

Sequences:

>Translated_99_residues
MASISIRCPSCSATEGVVRNGKSTAGHQRYLCSHCRKTWQLQFTYTASQPGTHQKIIDMAMNGVGYRASARIMGVGLNTV
LRHLKNSGRSGNLAHTTRQ
>Mature_98_residues
ASISIRCPSCSATEGVVRNGKSTAGHQRYLCSHCRKTWQLQFTYTASQPGTHQKIIDMAMNGVGYRASARIMGVGLNTVL
RHLKNSGRSGNLAHTTRQ

Specific function: Absolutely required for transposition of IS1 [H]

COG id: COG3677

COG function: function code L; Transposase and inactivated derivatives

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IS1 elements insA family [H]

Homologues:

Organism=Escherichia coli, GI1790747, Length=90, Percent_Identity=96.6666666666667, Blast_Score=179, Evalue=3e-47,
Organism=Escherichia coli, GI1789852, Length=90, Percent_Identity=93.3333333333333, Blast_Score=178, Evalue=5e-47,
Organism=Escherichia coli, GI1788204, Length=90, Percent_Identity=93.3333333333333, Blast_Score=178, Evalue=5e-47,
Organism=Escherichia coli, GI1786204, Length=90, Percent_Identity=93.3333333333333, Blast_Score=178, Evalue=5e-47,
Organism=Escherichia coli, GI87081814, Length=90, Percent_Identity=92.2222222222222, Blast_Score=177, Evalue=1e-46,
Organism=Escherichia coli, GI1786471, Length=90, Percent_Identity=92.2222222222222, Blast_Score=177, Evalue=1e-46,
Organism=Escherichia coli, GI1786460, Length=90, Percent_Identity=92.2222222222222, Blast_Score=177, Evalue=1e-46,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012287
- InterPro:   IPR003220 [H]

Pfam domain/function: PF03811 Ins_element1 [H]

EC number: NA

Molecular weight: Translated: 10821; Mature: 10689

Theoretical pI: Translated: 11.18; Mature: 11.18

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

4.0 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
8.1 %Cys+Met (Translated Protein)
4.1 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
7.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASISIRCPSCSATEGVVRNGKSTAGHQRYLCSHCRKTWQLQFTYTASQPGTHQKIIDMA
CCEEEEECCCCCCCCHHHHCCCCCCHHHHHHHHHCCCCEEEEEEEECCCCCCHHHHHHHH
MNGVGYRASARIMGVGLNTVLRHLKNSGRSGNLAHTTRQ
HCCCCCCCCCEEEEECHHHHHHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure 
ASISIRCPSCSATEGVVRNGKSTAGHQRYLCSHCRKTWQLQFTYTASQPGTHQKIIDMA
CEEEEECCCCCCCCHHHHCCCCCCHHHHHHHHHCCCCEEEEEEEECCCCCCHHHHHHHH
MNGVGYRASARIMGVGLNTVLRHLKNSGRSGNLAHTTRQ
HCCCCCCCCCEEEEECHHHHHHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 6265806 [H]