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The map label for this gene is priC

Identifier: 30061967

GI number: 30061967

Start: 426617

End: 427144

Strand: Reverse

Name: priC

Synonym: S0419

Alternate gene names: 30061967

Gene position: 427144-426617 (Counterclockwise)

Preceding gene: 30061976

Following gene: 30061966

Centisome position: 9.29

GC content: 59.09

Gene sequence:

>528_bases
GTGAAAACCGCCCTGCTGCTGGAAAAACTGGAAGGACAGCTCGCTACGCTGCGTCAGCGTTGTGCCCCGGTGTCACAGTT
CGCCACGCTAAGTGCTCGTTTCGACAGGCATCTTTTTCAGACTCGTGCGACAACACTACAGTCTTGTCTCGACGAGGCGG
GCGATAATCTGGCTGCGCTTCGTCATGCAGTTGAGCAGCAACAGCTGCCGCAAGTGGCCTGGCTGGCGGAACATCTGGCG
GCACAACTGGAAGCCATCGCGCGTGAAGCCTCCGCCTGGTCATTGCGCGAGTGGGACAGTGCGCCACCGAAAATTTCCCG
CTGGCAGCGTAAACGTATTCAGCATCAGGATTTTGAGCGGCGGCTACGTGAGATGGTTGCCGAACGCAGAGCCCGCCTGG
CGCGGGTGACCGATCTCGTGGAACAGCAAACGCTGCATCGTGAAGTGAAAGCCTATGAAGCGCGCCTGGCACGCTGCCGC
CATGCGCTGGAAAAAATCGAAAACAGGCTAGCGCGCTTAACCCGCTAG

Upstream 100 bases:

>100_bases
AGCCAATGATGATTAAAATGATTCGTTGCATATTGAGTGTTGTCAGCTTACATAGTGGCTACTTTAGCATAACAATTATC
ATTTTCATTGAGGTCTTATC

Downstream 100 bases:

>100_bases
CAATGGAAAGAGTATGTCACTTGAAAATGCCCCTGACGATGTCAAACTGGCTGTCGATTTGATTGTGCTGCTGGAGGAAA
ATCAGATCCCTGCCAGTACC

Product: primosomal replication protein N''

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 175; Mature: 175

Protein sequence:

>175_residues
MKTALLLEKLEGQLATLRQRCAPVSQFATLSARFDRHLFQTRATTLQSCLDEAGDNLAALRHAVEQQQLPQVAWLAEHLA
AQLEAIAREASAWSLREWDSAPPKISRWQRKRIQHQDFERRLREMVAERRARLARVTDLVEQQTLHREVKAYEARLARCR
HALEKIENRLARLTR

Sequences:

>Translated_175_residues
MKTALLLEKLEGQLATLRQRCAPVSQFATLSARFDRHLFQTRATTLQSCLDEAGDNLAALRHAVEQQQLPQVAWLAEHLA
AQLEAIAREASAWSLREWDSAPPKISRWQRKRIQHQDFERRLREMVAERRARLARVTDLVEQQTLHREVKAYEARLARCR
HALEKIENRLARLTR
>Mature_175_residues
MKTALLLEKLEGQLATLRQRCAPVSQFATLSARFDRHLFQTRATTLQSCLDEAGDNLAALRHAVEQQQLPQVAWLAEHLA
AQLEAIAREASAWSLREWDSAPPKISRWQRKRIQHQDFERRLREMVAERRARLARVTDLVEQQTLHREVKAYEARLARCR
HALEKIENRLARLTR

Specific function: PriA recognizes a specific hairpin sequence on bacteriophage phi X174 ssDNA. This structure is then recognized and bound by proteins priB and priC. Formation of the primosome proceeds with the subsequent actions of dnaB, dnaC, dnaT and primase [H]

COG id: COG3923

COG function: function code L; Primosomal replication protein N''

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1786673, Length=175, Percent_Identity=98.2857142857143, Blast_Score=338, Evalue=9e-95,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010890 [H]

Pfam domain/function: PF07445 priB_priC [H]

EC number: NA

Molecular weight: Translated: 20407; Mature: 20407

Theoretical pI: Translated: 10.94; Mature: 10.94

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTALLLEKLEGQLATLRQRCAPVSQFATLSARFDRHLFQTRATTLQSCLDEAGDNLAAL
CHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHH
RHAVEQQQLPQVAWLAEHLAAQLEAIAREASAWSLREWDSAPPKISRWQRKRIQHQDFER
HHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHH
RLREMVAERRARLARVTDLVEQQTLHREVKAYEARLARCRHALEKIENRLARLTR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKTALLLEKLEGQLATLRQRCAPVSQFATLSARFDRHLFQTRATTLQSCLDEAGDNLAAL
CHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHH
RHAVEQQQLPQVAWLAEHLAAQLEAIAREASAWSLREWDSAPPKISRWQRKRIQHQDFER
HHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHH
RLREMVAERRARLARVTDLVEQQTLHREVKAYEARLARCRHALEKIENRLARLTR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1856227; 9278503 [H]