Click here to switch to the map view.

The map label for this gene is ybaC

Identifier: 30061976

GI number: 30061976

Start: 435377

End: 436195

Strand: Reverse

Name: ybaC

Synonym: S0428

Alternate gene names: 30061976

Gene position: 436195-435377 (Counterclockwise)

Preceding gene: 30061978

Following gene: 30061967

Centisome position: 9.48

GC content: 50.79

Gene sequence:

>819_bases
ATGAAGCCGGAAAACAAACTACCTGTTCTGGACCTTATTTCTGCTGAAATGAAGACCGTTGTGAATACTCTTCAGCCGGA
TTTACCGTCCTGGCCCGCAACGGGAACGATTGCTGAGCAACGACAGTATTACACGCTTGAGCGCCGATTCTGGAATGCGG
GCGCTCCAGAAATGGCAACCAGAGCTTACATGGTTCCAACAAAATATGGGCAGGTGGAAACACGTCTCTTTTGTCCGCAG
CCAGATAGCCCGGCGACGCTATTTTATTTGCATGGAGGCGGTTTTATTCTCGGCAATCTCGATACCCACGATCGCATCAT
GCGCCTGCTGGCAAGCTACAGCCAATGTACGGTGATTGGTATTAATTACACCCTTTCACCTGAAGCGCGTTTTCCACAAG
CGATAGAGGAAATTGTGGCTGCTTGTTGTTATTTCCACCAGCAGGCGGAGGATTATCAAATCAATATGTCCCGCATTGGC
TTTGCCGGTGATTCCGCAGGTGCCATGCTGGCGCTCGCCAGTGCGTTGTGGTTGCGTGATAAACAGATCGATTGCGGTAA
AATTGCGGGTGTTTTGCTGTGGTATGGGCTTTACGGATTACGGGATTCCGTGACTCGTCGTCTGTTGGGCGGTGTCTGGG
ATGGCTTAACGCAACAGGATTTGCAGATGTATGAAGAGGCGTATTTAAGCAACGACGCGGACCGCGAGTCACCGTATTAC
TGTCTGTTTAATAATGATCTCACTCGCGAAGTTCCGCCCTGTTTTATTGCCGGGGCGGAGTTCGATCCGCTGCTGGATGA
CAGCCGTCTGCTTTACTAG

Upstream 100 bases:

>100_bases
GGGAGCGCGCGAGACGCAGCCAGTTTCAATATCAGCAGGCGGGATCGCATTTTCATGGCAGTGGTTCCTTACAATGACGT
AATTTGAAAGGAGTTTTTGT

Downstream 100 bases:

>100_bases
ACGTTAGCGGCGCATCAGCAGCCCTGTGAGTTCAAACTCTACCCAGGTACGCTGCATGCCTTTTTGCATTATTCACGGAT
GATGAAAACCGCCGACGAGG

Product: putative lipase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 272; Mature: 272

Protein sequence:

>272_residues
MKPENKLPVLDLISAEMKTVVNTLQPDLPSWPATGTIAEQRQYYTLERRFWNAGAPEMATRAYMVPTKYGQVETRLFCPQ
PDSPATLFYLHGGGFILGNLDTHDRIMRLLASYSQCTVIGINYTLSPEARFPQAIEEIVAACCYFHQQAEDYQINMSRIG
FAGDSAGAMLALASALWLRDKQIDCGKIAGVLLWYGLYGLRDSVTRRLLGGVWDGLTQQDLQMYEEAYLSNDADRESPYY
CLFNNDLTREVPPCFIAGAEFDPLLDDSRLLY

Sequences:

>Translated_272_residues
MKPENKLPVLDLISAEMKTVVNTLQPDLPSWPATGTIAEQRQYYTLERRFWNAGAPEMATRAYMVPTKYGQVETRLFCPQ
PDSPATLFYLHGGGFILGNLDTHDRIMRLLASYSQCTVIGINYTLSPEARFPQAIEEIVAACCYFHQQAEDYQINMSRIG
FAGDSAGAMLALASALWLRDKQIDCGKIAGVLLWYGLYGLRDSVTRRLLGGVWDGLTQQDLQMYEEAYLSNDADRESPYY
CLFNNDLTREVPPCFIAGAEFDPLLDDSRLLY
>Mature_272_residues
MKPENKLPVLDLISAEMKTVVNTLQPDLPSWPATGTIAEQRQYYTLERRFWNAGAPEMATRAYMVPTKYGQVETRLFCPQ
PDSPATLFYLHGGGFILGNLDTHDRIMRLLASYSQCTVIGINYTLSPEARFPQAIEEIVAACCYFHQQAEDYQINMSRIG
FAGDSAGAMLALASALWLRDKQIDCGKIAGVLLWYGLYGLRDSVTRRLLGGVWDGLTQQDLQMYEEAYLSNDADRESPYY
CLFNNDLTREVPPCFIAGAEFDPLLDDSRLLY

Specific function: Displays esterase activity towards short chain fatty esters (acyl chain length of up to 8 carbons). Able to hydrolyze triacetylglycerol (triacetin) and tributyrylglycerol (tributyrin), but not trioleylglycerol (triolein) or cholesterol oleate. Negatively

COG id: COG0657

COG function: function code I; Esterase/lipase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 'GDXG' lipolytic enzyme family [H]

Homologues:

Organism=Homo sapiens, GI68299767, Length=121, Percent_Identity=35.5371900826446, Blast_Score=72, Evalue=4e-13,
Organism=Escherichia coli, GI1786682, Length=272, Percent_Identity=98.8970588235294, Blast_Score=563, Evalue=1e-162,
Organism=Caenorhabditis elegans, GI17540028, Length=142, Percent_Identity=33.0985915492958, Blast_Score=74, Evalue=8e-14,
Organism=Caenorhabditis elegans, GI72001146, Length=127, Percent_Identity=32.2834645669291, Blast_Score=69, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI71996133, Length=209, Percent_Identity=28.2296650717703, Blast_Score=69, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24656084, Length=98, Percent_Identity=38.7755102040816, Blast_Score=79, Evalue=3e-15,
Organism=Drosophila melanogaster, GI24656076, Length=98, Percent_Identity=38.7755102040816, Blast_Score=79, Evalue=3e-15,
Organism=Drosophila melanogaster, GI20130169, Length=98, Percent_Identity=38.7755102040816, Blast_Score=79, Evalue=3e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013094
- InterPro:   IPR002168 [H]

Pfam domain/function: PF07859 Abhydrolase_3 [H]

EC number: 3.1.1.-

Molecular weight: Translated: 30671; Mature: 30671

Theoretical pI: Translated: 4.50; Mature: 4.50

Prosite motif: PS01173 LIPASE_GDXG_HIS ; PS01174 LIPASE_GDXG_SER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPENKLPVLDLISAEMKTVVNTLQPDLPSWPATGTIAEQRQYYTLERRFWNAGAPEMAT
CCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHH
RAYMVPTKYGQVETRLFCPQPDSPATLFYLHGGGFILGNLDTHDRIMRLLASYSQCTVIG
EEEECCCCCCCEEEEEECCCCCCCCEEEEEECCEEEEECCCHHHHHHHHHHCCCCEEEEE
INYTLSPEARFPQAIEEIVAACCYFHQQAEDYQINMSRIGFAGDSAGAMLALASALWLRD
EEEEECCCCCCHHHHHHHHHHHHHHHHCCCCEEEEHHHCCCCCCCCHHHHHHHHHHHHCC
KQIDCGKIAGVLLWYGLYGLRDSVTRRLLGGVWDGLTQQDLQMYEEAYLSNDADRESPYY
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCEE
CLFNNDLTREVPPCFIAGAEFDPLLDDSRLLY
EEECCCCCCCCCCEEEECCCCCCCCCCCCCCC
>Mature Secondary Structure
MKPENKLPVLDLISAEMKTVVNTLQPDLPSWPATGTIAEQRQYYTLERRFWNAGAPEMAT
CCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHH
RAYMVPTKYGQVETRLFCPQPDSPATLFYLHGGGFILGNLDTHDRIMRLLASYSQCTVIG
EEEECCCCCCCEEEEEECCCCCCCCEEEEEECCEEEEECCCHHHHHHHHHHCCCCEEEEE
INYTLSPEARFPQAIEEIVAACCYFHQQAEDYQINMSRIGFAGDSAGAMLALASALWLRD
EEEEECCCCCCHHHHHHHHHHHHHHHHCCCCEEEEHHHCCCCCCCCHHHHHHHHHHHHCC
KQIDCGKIAGVLLWYGLYGLRDSVTRRLLGGVWDGLTQQDLQMYEEAYLSNDADRESPYY
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCEE
CLFNNDLTREVPPCFIAGAEFDPLLDDSRLLY
EEECCCCCCCCCCEEEECCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on ester bonds; Carboxylic ester hydrolases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA