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The map label for this gene is ygfA [C]

Identifier: 29349666

GI number: 29349666

Start: 5608381

End: 5608971

Strand: Reverse

Name: ygfA [C]

Synonym: BT_4258

Alternate gene names: 29349666

Gene position: 5608971-5608381 (Counterclockwise)

Preceding gene: 29349667

Following gene: 29349665

Centisome position: 89.6

GC content: 48.73

Gene sequence:

>591_bases
ATGGAAAGAAAAAAAGAACTGCGCAAGCTTATGGCATCACTGAAGATCCGACACGCATCTTCGACTTTGCAGAAGCTACA
ATCAGCTAAAATACTTGCCGCCCTCGAAGCCCACCCGGCTTTCAGGGCGGCAACTACTGTATTGCTCTACCATTCGCTCA
AAGATGAAGTAGACACGCATGCGTTTATCCGGAAATGGAGCAATGAGAAGCGGATATTGCTTCCGGTAGTTGTCGGTGAT
GAATTGGAATTACGCCTCTATACCGGTCCCGAGGATATGGCAACCGGCACTTACGGCATCGAAGAGCCTGTTGGAGAAAC
ATTCACAGACTATGCAGACATAGACTTTATTGCCGTTCCCGGTGTAGCTTTCGACCGGAAAGGAAACCGGCTGGGAAGGG
GAAAAGGCTATTATGACCGTTTGCTGCCACGTATCCCCGCAGCTTATAAAGCAGGCATCTGCTTCCCATTTCAAATAGTA
GAAGAAGTTCCGGCAGAAGCATTCGACATCTGCATGGACATAATTATAACAAGTAATGAGGACGAATTATCACACCCACA
CCACCCGCTGCCTCCATGCGACAGGGAGTGA

Upstream 100 bases:

>100_bases
GATGCTACAGTTCAACAAGGTATAGAACTGCTCGAAAAAGGAGAAGCTTTCCCAAAAGCACCCGCCGAGACAGAAGAGGA
AGTGACGAAAGACAATAAAG

Downstream 100 bases:

>100_bases
CGAAGAATTTGTTTTAAGCGCCATCAAAGGCGGCTATCAGGAACTTGGTTTCTCTGACCACACACCATGGGAATACCATA
CCGATTATGTATCCGACATC

Product: putative 5-formyltetrahydrofolate cyclo-ligase

Products: ADP; phosphate; 5,10-methenyltetrahydrofolate

Alternate protein names: NA

Number of amino acids: Translated: 196; Mature: 196

Protein sequence:

>196_residues
MERKKELRKLMASLKIRHASSTLQKLQSAKILAALEAHPAFRAATTVLLYHSLKDEVDTHAFIRKWSNEKRILLPVVVGD
ELELRLYTGPEDMATGTYGIEEPVGETFTDYADIDFIAVPGVAFDRKGNRLGRGKGYYDRLLPRIPAAYKAGICFPFQIV
EEVPAEAFDICMDIIITSNEDELSHPHHPLPPCDRE

Sequences:

>Translated_196_residues
MERKKELRKLMASLKIRHASSTLQKLQSAKILAALEAHPAFRAATTVLLYHSLKDEVDTHAFIRKWSNEKRILLPVVVGD
ELELRLYTGPEDMATGTYGIEEPVGETFTDYADIDFIAVPGVAFDRKGNRLGRGKGYYDRLLPRIPAAYKAGICFPFQIV
EEVPAEAFDICMDIIITSNEDELSHPHHPLPPCDRE
>Mature_196_residues
MERKKELRKLMASLKIRHASSTLQKLQSAKILAALEAHPAFRAATTVLLYHSLKDEVDTHAFIRKWSNEKRILLPVVVGD
ELELRLYTGPEDMATGTYGIEEPVGETFTDYADIDFIAVPGVAFDRKGNRLGRGKGYYDRLLPRIPAAYKAGICFPFQIV
EEVPAEAFDICMDIIITSNEDELSHPHHPLPPCDRE

Specific function: Unknown

COG id: COG0212

COG function: function code H; 5-formyltetrahydrofolate cyclo-ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 5-formyltetrahydrofolate cyclo-ligase family [H]

Homologues:

Organism=Homo sapiens, GI5453746, Length=186, Percent_Identity=27.9569892473118, Blast_Score=74, Evalue=1e-13,
Organism=Escherichia coli, GI1789278, Length=156, Percent_Identity=31.4102564102564, Blast_Score=65, Evalue=3e-12,
Organism=Drosophila melanogaster, GI161077430, Length=170, Percent_Identity=30.5882352941176, Blast_Score=72, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002698 [H]

Pfam domain/function: PF01812 5-FTHF_cyc-lig [H]

EC number: 6.3.3.2

Molecular weight: Translated: 22092; Mature: 22092

Theoretical pI: Translated: 6.16; Mature: 6.16

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MERKKELRKLMASLKIRHASSTLQKLQSAKILAALEAHPAFRAATTVLLYHSLKDEVDTH
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
AFIRKWSNEKRILLPVVVGDELELRLYTGPEDMATGTYGIEEPVGETFTDYADIDFIAVP
HHHHHHCCCCEEEEEEEECCCEEEEEEECCHHHCCCCCCCCCCCCCHHHCCCCCCEEEEC
GVAFDRKGNRLGRGKGYYDRLLPRIPAAYKAGICFPFQIVEEVPAEAFDICMDIIITSNE
CEEECCCCCCCCCCCCHHHHHHHHCCHHHHCCCCCHHHHHHHCCHHHHHHHHHHHEECCC
DELSHPHHPLPPCDRE
HHCCCCCCCCCCCCCC
>Mature Secondary Structure
MERKKELRKLMASLKIRHASSTLQKLQSAKILAALEAHPAFRAATTVLLYHSLKDEVDTH
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
AFIRKWSNEKRILLPVVVGDELELRLYTGPEDMATGTYGIEEPVGETFTDYADIDFIAVP
HHHHHHCCCCEEEEEEEECCCEEEEEEECCHHHCCCCCCCCCCCCCHHHCCCCCCEEEEC
GVAFDRKGNRLGRGKGYYDRLLPRIPAAYKAGICFPFQIVEEVPAEAFDICMDIIITSNE
CEEECCCCCCCCCCCCHHHHHHHHCCHHHHCCCCCHHHHHHHCCHHHHHHHHHHHEECCC
DELSHPHHPLPPCDRE
HHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; 5-formyltetrahydrofolate

Specific reaction: ATP + 5-formyltetrahydrofolate = ADP + phosphate + 5,10-methenyltetrahydrofolate

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12089438 [H]