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The map label for this gene is ubiE

Identifier: 29349624

GI number: 29349624

Start: 5552878

End: 5553615

Strand: Reverse

Name: ubiE

Synonym: BT_4216

Alternate gene names: 29349624

Gene position: 5553615-5552878 (Counterclockwise)

Preceding gene: 29349625

Following gene: 29349623

Centisome position: 88.71

GC content: 48.1

Gene sequence:

>738_bases
ATGGATTACCCACAACAACATATCAAGCCTTACAACGAAGACGGTAAGAAAACCGAGCAGGTGGAACGTATGTTCGATAA
CATTGCGCATGCTTACGACAAACTGAATCATACCTTATCTTTAGGTATAGACCGCAGCTGGCGCCGTAAAGCTATTGCCT
GGCTCCGTCCCTTCCGCCCACAGCACATCATGGATGTTGCCACCGGAACAGGAGACTTCGCCATCCTCGCTTGCCGCGAA
CTGAATCCTGACGAACTGATCGGAACGGATATTTCGGAAGGCATGATGAACGTGGGTCGTGAAAAAGTAAAGAAGGAAGG
ACTCTCGGACAAAATTTCTTTTGCCCGGGAGGACTGCACTTCTCTCTCCTTTGCCGACAATAGATTTGACGCTATCACCG
TAGCATTCGGTATCCGCAATTTTGAAGACCTGGACAAAGGCCTCTCCGAAATGTGTCGTGTACTGAAACCGGGCGGACAT
CTGGTTATCCTGGAACTGACTACTCCGGATCGCTTCCCCATGAAGCAACTGTTCGCCATCTACTCGAAAGTAGTTATCCC
ATTGCTTGGCAAACTCCTCTCCAAAGACAACAGTGCCTACCATTATCTGCCGGACACTATCAAAGTATTTCCCCAAGGGG
AAGTCATGAAAGGAGTCATATCGAAAACCGGATTCGGCGAAGTTCATTTCCGACGGTTGACATTTGGTATCTGCACACTT
TATACAGCGACAAAATAA

Upstream 100 bases:

>100_bases
TGTTCGAGAATATCACCGGTGAGAAATTTGTGAAAGAAGATACCAGCAACATCGCTGAACGTATCGAAAAGAACGTTATG
AATTTCTTGAGCAAATAAGC

Downstream 100 bases:

>100_bases
TGAAGAATGATGAATAATTAATTTAATCCTTTCGTGTCATGGAAAAATACGGTTTAATCGGCTACCCCTTGCGACATTCG
TTTTCAATCGGCTACTTTAA

Product: ubiquinone/menaquinone biosynthesis methyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 245; Mature: 245

Protein sequence:

>245_residues
MDYPQQHIKPYNEDGKKTEQVERMFDNIAHAYDKLNHTLSLGIDRSWRRKAIAWLRPFRPQHIMDVATGTGDFAILACRE
LNPDELIGTDISEGMMNVGREKVKKEGLSDKISFAREDCTSLSFADNRFDAITVAFGIRNFEDLDKGLSEMCRVLKPGGH
LVILELTTPDRFPMKQLFAIYSKVVIPLLGKLLSKDNSAYHYLPDTIKVFPQGEVMKGVISKTGFGEVHFRRLTFGICTL
YTATK

Sequences:

>Translated_245_residues
MDYPQQHIKPYNEDGKKTEQVERMFDNIAHAYDKLNHTLSLGIDRSWRRKAIAWLRPFRPQHIMDVATGTGDFAILACRE
LNPDELIGTDISEGMMNVGREKVKKEGLSDKISFAREDCTSLSFADNRFDAITVAFGIRNFEDLDKGLSEMCRVLKPGGH
LVILELTTPDRFPMKQLFAIYSKVVIPLLGKLLSKDNSAYHYLPDTIKVFPQGEVMKGVISKTGFGEVHFRRLTFGICTL
YTATK
>Mature_245_residues
MDYPQQHIKPYNEDGKKTEQVERMFDNIAHAYDKLNHTLSLGIDRSWRRKAIAWLRPFRPQHIMDVATGTGDFAILACRE
LNPDELIGTDISEGMMNVGREKVKKEGLSDKISFAREDCTSLSFADNRFDAITVAFGIRNFEDLDKGLSEMCRVLKPGGH
LVILELTTPDRFPMKQLFAIYSKVVIPLLGKLLSKDNSAYHYLPDTIKVFPQGEVMKGVISKTGFGEVHFRRLTFGICTL
YTATK

Specific function: Methyltransferase required for the conversion of dimethylmenaquinone (DMKH2) to menaquinone (MKH2)

COG id: COG2226

COG function: function code H; Methylase involved in ubiquinone/menaquinone biosynthesis

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. UbiE family

Homologues:

Organism=Homo sapiens, GI116063536, Length=268, Percent_Identity=32.4626865671642, Blast_Score=145, Evalue=3e-35,
Organism=Escherichia coli, GI48994983, Length=231, Percent_Identity=35.4978354978355, Blast_Score=164, Evalue=4e-42,
Organism=Caenorhabditis elegans, GI17557039, Length=235, Percent_Identity=32.3404255319149, Blast_Score=149, Evalue=1e-36,
Organism=Saccharomyces cerevisiae, GI6323526, Length=248, Percent_Identity=34.2741935483871, Blast_Score=122, Evalue=4e-29,
Organism=Drosophila melanogaster, GI24641702, Length=246, Percent_Identity=36.1788617886179, Blast_Score=166, Evalue=2e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): UBIE_BACTN (Q8A005)

Other databases:

- EMBL:   AE015928
- RefSeq:   NP_813127.1
- ProteinModelPortal:   Q8A005
- SMR:   Q8A005
- GeneID:   1074556
- GenomeReviews:   AE015928_GR
- KEGG:   bth:BT_4216
- NMPDR:   fig|226186.1.peg.4214
- HOGENOM:   HBG559580
- OMA:   FDVYTIA
- PhylomeDB:   Q8A005
- ProtClustDB:   PRK00216
- BioCyc:   BTHE226186:BT_4216-MONOMER
- HAMAP:   MF_01813
- InterPro:   IPR004033
- PANTHER:   PTHR10108:SF24
- TIGRFAMs:   TIGR01934

Pfam domain/function: PF01209 Ubie_methyltran

EC number: 2.1.1.-

Molecular weight: Translated: 27821; Mature: 27821

Theoretical pI: Translated: 8.32; Mature: 8.32

Prosite motif: PS01183 UBIE_1; PS01184 UBIE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDYPQQHIKPYNEDGKKTEQVERMFDNIAHAYDKLNHTLSLGIDRSWRRKAIAWLRPFRP
CCCCHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEECCCCHHHHHHHHHHCCCCC
QHIMDVATGTGDFAILACRELNPDELIGTDISEGMMNVGREKVKKEGLSDKISFAREDCT
HHHHHHHCCCCCEEEEEECCCCHHHHHCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHC
SLSFADNRFDAITVAFGIRNFEDLDKGLSEMCRVLKPGGHLVILELTTPDRFPMKQLFAI
CCCCCCCCHHEEEEEHHCCCHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCHHHHHHH
YSKVVIPLLGKLLSKDNSAYHYLPDTIKVFPQGEVMKGVISKTGFGEVHFRRLTFGICTL
HHHHHHHHHHHHHCCCCCCEEECCHHHEECCCCHHHHHHHHCCCCCHHHHHHHHHHHHHH
YTATK
HCCCC
>Mature Secondary Structure
MDYPQQHIKPYNEDGKKTEQVERMFDNIAHAYDKLNHTLSLGIDRSWRRKAIAWLRPFRP
CCCCHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEECCCCHHHHHHHHHHCCCCC
QHIMDVATGTGDFAILACRELNPDELIGTDISEGMMNVGREKVKKEGLSDKISFAREDCT
HHHHHHHCCCCCEEEEEECCCCHHHHHCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHC
SLSFADNRFDAITVAFGIRNFEDLDKGLSEMCRVLKPGGHLVILELTTPDRFPMKQLFAI
CCCCCCCCHHEEEEEHHCCCHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCHHHHHHH
YSKVVIPLLGKLLSKDNSAYHYLPDTIKVFPQGEVMKGVISKTGFGEVHFRRLTFGICTL
HHHHHHHHHHHHHCCCCCCEEECCHHHEECCCCHHHHHHHHCCCCCHHHHHHHHHHHHHH
YTATK
HCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring one-carbon groups; Methyltransferases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12663928