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The map label for this gene is prfA [H]

Identifier: 29349618

GI number: 29349618

Start: 5546853

End: 5547965

Strand: Reverse

Name: prfA [H]

Synonym: BT_4210

Alternate gene names: 29349618

Gene position: 5547965-5546853 (Counterclockwise)

Preceding gene: 29349619

Following gene: 29349617

Centisome position: 88.62

GC content: 46.81

Gene sequence:

>1113_bases
ATGGCAGATAACAGTACCATATTAGAAAAACTAGACGGACTTGTAGCCCGTTTTGAAGAAATATCTACCCTCATCACCGA
CCCGGCAGTAATTGCCGACCAGAAACGTTATGTCAAACTGACCAAAGAATACAAGGATCTGGATGACTTGATGAAAGCCC
GCAAAGAATATATCCAACTGTTAGGCAACATCGAGGAAGCCAAAAACATCCTTTCGAATGAGTCGGACGCTGACATGCGT
GAAATGGCAAAAGAGGAGATGGACAACAGTCAGGAACGTCTTCCGGCATTAGAAGAAGAAATCAAACTAATGCTCGTTCC
TGCCGATCCGCAAGACAGCAAGAACGCCATCCTCGAAATCCGTGGTGGTGCAGGAGGAGACGAAGCCGCAATCTTTGCCG
GCGACCTTTTCCGTATGTATGCCAAGTTCTGTGAAACCAAAGGCTGGAAAATGGAAGTATCCAATGCAAATGAAGGAACA
GCCGGAGGATACAAAGAAATTGTTTGCAGCGTCACAGGAGATAATGTCTACGGAATATTGAAATATGAATCGGGTGTACA
CCGCGTACAACGTGTTCCTGCCACAGAGACACAAGGACGTGTGCATACTTCCGCCGCTTCGGTGGCCGTACTCCCCGAAG
CCGAAGAATTTGACGTAGTCATCAATGAAGGAGAAATCAAATGGGATACCTTCCGAAGTGGCGGTGCAGGCGGACAGAAC
GTTAATAAGGTAGAATCCGGTGTACGCTTGCGTTATATCTGGAAGAACCCGAATACAGGCGTTGCAGAAGAAATCCTGAT
TGAATGTACAGAAACCCGTGACCAACCGAAGAACAAGGAACGCGCCCTGGCACGACTCCGTACCTTTATCTACGACAAGG
AACATCAGAAGTACATAGACGACATAGCTTCCAAACGTAAGACAATGGTATCTACCGGCGACCGCTCGGCTAAAATCCGG
ACATACAACTACCCTCAAGGACGTATCACCGATCATCGTATCAACTATACCATCTATAATCTTGCCGCTTTTATGGACGG
AGATATTCAAGATTGTATCGATCACCTGATCGTGGCAGAAAATGCGGAACGCCTGAAAGAAAGTGAACTCTAA

Upstream 100 bases:

>100_bases
ATCATTCGGCATTCCTGCACAGATAGTAGGACGTATCGAAGCGTGTGACCAGACTGAATTAATCATTAAAAGCGAGTTCG
GAGAATTCAGATACTAAAGA

Downstream 100 bases:

>100_bases
AAATTATAGTATAGACATGAATAAACAACAGCTATTTGAAAACATAAAACGCAAGAAATCATTCCTTTGCGTAGGGCTTG
ATACCGACATCAAGAAGATT

Product: peptide chain release factor 1

Products: NA

Alternate protein names: RF-1 [H]

Number of amino acids: Translated: 370; Mature: 369

Protein sequence:

>370_residues
MADNSTILEKLDGLVARFEEISTLITDPAVIADQKRYVKLTKEYKDLDDLMKARKEYIQLLGNIEEAKNILSNESDADMR
EMAKEEMDNSQERLPALEEEIKLMLVPADPQDSKNAILEIRGGAGGDEAAIFAGDLFRMYAKFCETKGWKMEVSNANEGT
AGGYKEIVCSVTGDNVYGILKYESGVHRVQRVPATETQGRVHTSAASVAVLPEAEEFDVVINEGEIKWDTFRSGGAGGQN
VNKVESGVRLRYIWKNPNTGVAEEILIECTETRDQPKNKERALARLRTFIYDKEHQKYIDDIASKRKTMVSTGDRSAKIR
TYNYPQGRITDHRINYTIYNLAAFMDGDIQDCIDHLIVAENAERLKESEL

Sequences:

>Translated_370_residues
MADNSTILEKLDGLVARFEEISTLITDPAVIADQKRYVKLTKEYKDLDDLMKARKEYIQLLGNIEEAKNILSNESDADMR
EMAKEEMDNSQERLPALEEEIKLMLVPADPQDSKNAILEIRGGAGGDEAAIFAGDLFRMYAKFCETKGWKMEVSNANEGT
AGGYKEIVCSVTGDNVYGILKYESGVHRVQRVPATETQGRVHTSAASVAVLPEAEEFDVVINEGEIKWDTFRSGGAGGQN
VNKVESGVRLRYIWKNPNTGVAEEILIECTETRDQPKNKERALARLRTFIYDKEHQKYIDDIASKRKTMVSTGDRSAKIR
TYNYPQGRITDHRINYTIYNLAAFMDGDIQDCIDHLIVAENAERLKESEL
>Mature_369_residues
ADNSTILEKLDGLVARFEEISTLITDPAVIADQKRYVKLTKEYKDLDDLMKARKEYIQLLGNIEEAKNILSNESDADMRE
MAKEEMDNSQERLPALEEEIKLMLVPADPQDSKNAILEIRGGAGGDEAAIFAGDLFRMYAKFCETKGWKMEVSNANEGTA
GGYKEIVCSVTGDNVYGILKYESGVHRVQRVPATETQGRVHTSAASVAVLPEAEEFDVVINEGEIKWDTFRSGGAGGQNV
NKVESGVRLRYIWKNPNTGVAEEILIECTETRDQPKNKERALARLRTFIYDKEHQKYIDDIASKRKTMVSTGDRSAKIRT
YNYPQGRITDHRINYTIYNLAAFMDGDIQDCIDHLIVAENAERLKESEL

Specific function: Peptide chain release factor 1 directs the termination of translation in response to the peptide chain termination codons UAG and UAA [H]

COG id: COG0216

COG function: function code J; Protein chain release factor A

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family [H]

Homologues:

Organism=Homo sapiens, GI166795303, Length=294, Percent_Identity=40.4761904761905, Blast_Score=235, Evalue=6e-62,
Organism=Homo sapiens, GI34577120, Length=294, Percent_Identity=36.734693877551, Blast_Score=191, Evalue=9e-49,
Organism=Homo sapiens, GI166795305, Length=195, Percent_Identity=40, Blast_Score=160, Evalue=2e-39,
Organism=Escherichia coli, GI1787462, Length=361, Percent_Identity=45.983379501385, Blast_Score=325, Evalue=3e-90,
Organism=Escherichia coli, GI2367172, Length=347, Percent_Identity=31.9884726224784, Blast_Score=162, Evalue=4e-41,
Organism=Caenorhabditis elegans, GI17542784, Length=296, Percent_Identity=35.8108108108108, Blast_Score=181, Evalue=5e-46,
Organism=Saccharomyces cerevisiae, GI6321295, Length=336, Percent_Identity=34.2261904761905, Blast_Score=185, Evalue=1e-47,
Organism=Drosophila melanogaster, GI19921226, Length=341, Percent_Identity=36.6568914956012, Blast_Score=195, Evalue=4e-50,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR004373 [H]

Pfam domain/function: PF03462 PCRF; PF00472 RF-1 [H]

EC number: NA

Molecular weight: Translated: 41662; Mature: 41531

Theoretical pI: Translated: 4.72; Mature: 4.72

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADNSTILEKLDGLVARFEEISTLITDPAVIADQKRYVKLTKEYKDLDDLMKARKEYIQL
CCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCHHHHHHHHHHCCHHHHHHHHHHHHHH
LGNIEEAKNILSNESDADMREMAKEEMDNSQERLPALEEEIKLMLVPADPQDSKNAILEI
HCCHHHHHHHHCCCCCHHHHHHHHHHHCCHHHHCCCHHHCCEEEEEECCCCCCCCCEEEE
RGGAGGDEAAIFAGDLFRMYAKFCETKGWKMEVSNANEGTAGGYKEIVCSVTGDNVYGIL
ECCCCCCCCEEHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCHHHEEEEEECCCEEEEE
KYESGVHRVQRVPATETQGRVHTSAASVAVLPEAEEFDVVINEGEIKWDTFRSGGAGGQN
EECCHHHHHHCCCCCCCCCCEEECCCCEEECCCCCCCCEEEECCEEEEECCCCCCCCCCC
VNKVESGVRLRYIWKNPNTGVAEEILIECTETRDQPKNKERALARLRTFIYDKEHQKYID
HHHHHCCCEEEEEEECCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCHHHHHHH
DIASKRKTMVSTGDRSAKIRTYNYPQGRITDHRINYTIYNLAAFMDGDIQDCIDHLIVAE
HHHHHHHHHHCCCCCCCEEEEEECCCCCEECCEECEEEEEHHHHHCCCHHHHHHHHHHHC
NAERLKESEL
CHHHHHHCCC
>Mature Secondary Structure 
ADNSTILEKLDGLVARFEEISTLITDPAVIADQKRYVKLTKEYKDLDDLMKARKEYIQL
CCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCHHHHHHHHHHCCHHHHHHHHHHHHHH
LGNIEEAKNILSNESDADMREMAKEEMDNSQERLPALEEEIKLMLVPADPQDSKNAILEI
HCCHHHHHHHHCCCCCHHHHHHHHHHHCCHHHHCCCHHHCCEEEEEECCCCCCCCCEEEE
RGGAGGDEAAIFAGDLFRMYAKFCETKGWKMEVSNANEGTAGGYKEIVCSVTGDNVYGIL
ECCCCCCCCEEHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCHHHEEEEEECCCEEEEE
KYESGVHRVQRVPATETQGRVHTSAASVAVLPEAEEFDVVINEGEIKWDTFRSGGAGGQN
EECCHHHHHHCCCCCCCCCCEEECCCCEEECCCCCCCCEEEECCEEEEECCCCCCCCCCC
VNKVESGVRLRYIWKNPNTGVAEEILIECTETRDQPKNKERALARLRTFIYDKEHQKYID
HHHHHCCCEEEEEEECCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCHHHHHHH
DIASKRKTMVSTGDRSAKIRTYNYPQGRITDHRINYTIYNLAAFMDGDIQDCIDHLIVAE
HHHHHHHHHHCCCCCCCEEEEEECCCCCEECCEECEEEEEHHHHHCCCHHHHHHHHHHHC
NAERLKESEL
CHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA