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The map label for this gene is lipA

Identifier: 29349600

GI number: 29349600

Start: 5527126

End: 5527974

Strand: Reverse

Name: lipA

Synonym: BT_4192

Alternate gene names: 29349600

Gene position: 5527974-5527126 (Counterclockwise)

Preceding gene: 29349601

Following gene: 29349599

Centisome position: 88.3

GC content: 50.29

Gene sequence:

>849_bases
ATGGCTGACAGAGTGCGCAAGCCTGAATGGCTGAAAATAAATATCGGTGCCAACGACCGTTACACCGAAACTAAAAGAAT
TGTCGACTCCCACTGCCTGCATACGATATGCAGCAGTGGGCGTTGTCCCAACATGGGAGAATGCTGGGGAAAAGGAACTG
CCACATTTATGATCGGCGGTGACATCTGTACCCGCAGCTGCAAATTCTGCAACACACAAACCGGACGCCCGCATCCACTG
GATGCCAACGAACCGACACACGTAGCGGAGTCTATCGCTCTTATGAAACTAGATCATGCGGTAGTTACCTCGGTAGACCG
TGACGACCTTCCCGATCTGGGAGCCGGACATTGGGCACATACCATTCGTGAGATCAAACGACTGAATCCGCAGACCACAA
TCGAAGTACTGATACCCGATTTTCAGGGTAGAATGGAACTGGTAGACCTAGTGATAGAAGCCAATCCCGACATCATTTCC
CATAATATGGAGACGGTAAGACGCATCAGTCCCCTGGTTCGCAGTGCGGCCAACTATGATACCAGCCTGCAAGTGATCGG
TCACATCGCCCGAAGCGGAACAAAATCAAAAAGCGGTATTATGGTGGGGTTGGGAGAAACTCCACAGGAAGTGGAAACCA
TTATGGACGACCTGCTGGCAGTCGGCTGCCAGATTCTTACGATAGGACAATATTTGCAGCCTACGCACCGTCATTATCCG
GTTGCCGAGTATGTGACGCCACAGCAATTCGCCACATACAAAACCATCGGTTTGGAGAAAGGATTCAGCATTGTAGAGAG
TGCTCCTCTCGTTCGTTCGTCTTATCATGCAGAGAAACACATTCGATAG

Upstream 100 bases:

>100_bases
ATATGCAAGTATATACCAATCGTAATCATGGCATATACGGCGGCAACACCCGTCAGCATCTATATACACGATTGACCAAC
TTCTTCCTGAACAATTTGTA

Downstream 100 bases:

>100_bases
AAAACAAAACACATTCACTATTTGTTTTATAGGTAAAACCGGCAAATAAACATAAAGATGGATCATAAAGGAACACTTTC
TTCCGCGTTTAACATGTCAT

Product: lipoyl synthase

Products: NA

Alternate protein names: Lip-syn; LS; Lipoate synthase; Lipoic acid synthase; Sulfur insertion protein lipA

Number of amino acids: Translated: 282; Mature: 281

Protein sequence:

>282_residues
MADRVRKPEWLKINIGANDRYTETKRIVDSHCLHTICSSGRCPNMGECWGKGTATFMIGGDICTRSCKFCNTQTGRPHPL
DANEPTHVAESIALMKLDHAVVTSVDRDDLPDLGAGHWAHTIREIKRLNPQTTIEVLIPDFQGRMELVDLVIEANPDIIS
HNMETVRRISPLVRSAANYDTSLQVIGHIARSGTKSKSGIMVGLGETPQEVETIMDDLLAVGCQILTIGQYLQPTHRHYP
VAEYVTPQQFATYKTIGLEKGFSIVESAPLVRSSYHAEKHIR

Sequences:

>Translated_282_residues
MADRVRKPEWLKINIGANDRYTETKRIVDSHCLHTICSSGRCPNMGECWGKGTATFMIGGDICTRSCKFCNTQTGRPHPL
DANEPTHVAESIALMKLDHAVVTSVDRDDLPDLGAGHWAHTIREIKRLNPQTTIEVLIPDFQGRMELVDLVIEANPDIIS
HNMETVRRISPLVRSAANYDTSLQVIGHIARSGTKSKSGIMVGLGETPQEVETIMDDLLAVGCQILTIGQYLQPTHRHYP
VAEYVTPQQFATYKTIGLEKGFSIVESAPLVRSSYHAEKHIR
>Mature_281_residues
ADRVRKPEWLKINIGANDRYTETKRIVDSHCLHTICSSGRCPNMGECWGKGTATFMIGGDICTRSCKFCNTQTGRPHPLD
ANEPTHVAESIALMKLDHAVVTSVDRDDLPDLGAGHWAHTIREIKRLNPQTTIEVLIPDFQGRMELVDLVIEANPDIISH
NMETVRRISPLVRSAANYDTSLQVIGHIARSGTKSKSGIMVGLGETPQEVETIMDDLLAVGCQILTIGQYLQPTHRHYPV
AEYVTPQQFATYKTIGLEKGFSIVESAPLVRSSYHAEKHIR

Specific function: Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives

COG id: COG0320

COG function: function code H; Lipoate synthase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Lipoyl synthase family

Homologues:

Organism=Homo sapiens, GI37577166, Length=285, Percent_Identity=45.2631578947368, Blast_Score=256, Evalue=1e-68,
Organism=Homo sapiens, GI37577164, Length=249, Percent_Identity=44.578313253012, Blast_Score=217, Evalue=1e-56,
Organism=Escherichia coli, GI1786846, Length=278, Percent_Identity=47.1223021582734, Blast_Score=259, Evalue=1e-70,
Organism=Caenorhabditis elegans, GI32564533, Length=286, Percent_Identity=42.3076923076923, Blast_Score=224, Evalue=5e-59,
Organism=Saccharomyces cerevisiae, GI6324770, Length=280, Percent_Identity=44.2857142857143, Blast_Score=239, Evalue=5e-64,
Organism=Drosophila melanogaster, GI221513272, Length=283, Percent_Identity=46.9964664310954, Blast_Score=263, Evalue=1e-70,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LIPA_BACTN (Q8A029)

Other databases:

- EMBL:   AE015928
- RefSeq:   NP_813103.1
- ProteinModelPortal:   Q8A029
- GeneID:   1072478
- GenomeReviews:   AE015928_GR
- KEGG:   bth:BT_4192
- NMPDR:   fig|226186.1.peg.4190
- HOGENOM:   HBG284542
- OMA:   TTIEVLI
- PhylomeDB:   Q8A029
- ProtClustDB:   PRK05481
- BioCyc:   BTHE226186:BT_4192-MONOMER
- BRENDA:   2.8.1.8
- GO:   GO:0005737
- HAMAP:   MF_00206
- InterPro:   IPR013785
- InterPro:   IPR006638
- InterPro:   IPR003698
- InterPro:   IPR007197
- Gene3D:   G3DSA:3.20.20.70
- PIRSF:   PIRSF005963
- SMART:   SM00729
- TIGRFAMs:   TIGR00510

Pfam domain/function: PF04055 Radical_SAM

EC number: =2.8.1.8

Molecular weight: Translated: 31352; Mature: 31221

Theoretical pI: Translated: 6.98; Mature: 6.98

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADRVRKPEWLKINIGANDRYTETKRIVDSHCLHTICSSGRCPNMGECWGKGTATFMIGG
CCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHCCCCEEEEECC
DICTRSCKFCNTQTGRPHPLDANEPTHVAESIALMKLDHAVVTSVDRDDLPDLGAGHWAH
HHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHH
TIREIKRLNPQTTIEVLIPDFQGRMELVDLVIEANPDIISHNMETVRRISPLVRSAANYD
HHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHCCCCHHHCCHHHHHHHHHHHHHHHCCC
TSLQVIGHIARSGTKSKSGIMVGLGETPQEVETIMDDLLAVGCQILTIGQYLQPTHRHYP
HHHHHHHHHHHCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHCHHHCCCC
VAEYVTPQQFATYKTIGLEKGFSIVESAPLVRSSYHAEKHIR
HHHHCCHHHHHHHHHHHHHCCCHHHHCCCHHHHHHHHHHCCC
>Mature Secondary Structure 
ADRVRKPEWLKINIGANDRYTETKRIVDSHCLHTICSSGRCPNMGECWGKGTATFMIGG
CCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHCCCCEEEEECC
DICTRSCKFCNTQTGRPHPLDANEPTHVAESIALMKLDHAVVTSVDRDDLPDLGAGHWAH
HHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHH
TIREIKRLNPQTTIEVLIPDFQGRMELVDLVIEANPDIISHNMETVRRISPLVRSAANYD
HHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHCCCCHHHCCHHHHHHHHHHHHHHHCCC
TSLQVIGHIARSGTKSKSGIMVGLGETPQEVETIMDDLLAVGCQILTIGQYLQPTHRHYP
HHHHHHHHHHHCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHCHHHCCCC
VAEYVTPQQFATYKTIGLEKGFSIVESAPLVRSSYHAEKHIR
HHHHCCHHHHHHHHHHHHHCCCHHHHCCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12663928