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The map label for this gene is nuoB

Identifier: 29349474

GI number: 29349474

Start: 5300292

End: 5300885

Strand: Reverse

Name: nuoB

Synonym: BT_4066

Alternate gene names: 29349474

Gene position: 5300885-5300292 (Counterclockwise)

Preceding gene: 29349475

Following gene: 29349473

Centisome position: 84.67

GC content: 47.31

Gene sequence:

>594_bases
ATGGAAATAACCAAAAAACCGAAAATAAAATCAATTCCGTATGATGAGTTCATCGACAATGAATCATTGGAAAAGTTGGT
CAAGGAACTTAATACCGGAGGAGCGAACGTTTTTCTTGGGGTTCTGGACGACCTTGTCAACTGGGGACGCAGCAATTCAC
TGTGGCCGCTTACTTTCGCAACCAGCTGTTGCGGCATCGAATTCATGGCACTGGGTGCCGCGCGTTATGATATGGCCCGC
TTCGGGTTCGAAGTAGCACGTGCCAGTCCGCGCCAAGCTGATATGATCATGGTCTGCGGAACAATCACCAACAAGATGGC
ACCGGTACTGAAACGTTTGTACGATCAGATGCCCGATCCGAAATATGTAGTTGCCGTAGGCGGATGTGCTGTCAGCGGAG
GGCCTTTCAAGAAGTCTTATCATGTGCTGAATGGAGTAGATAAGATTCTTCCGGTCGATGTATATATCCCCGGATGTCCG
CCCCGCCCGGAAGCTTTCTATTACGGCATGATGCAACTACAACGCAAAGTGAAAATAGAGAAATTCTTTGGTGGAACGAA
CAGAAAAGAGAAGAAACCGGAATTTATGAAATGA

Upstream 100 bases:

>100_bases
TTATGCATGACATGGGACCTCAGGGGCTCGTTAGTATTCTCTTCTTCTTTATTATTTTGGTTCTGGGCCTTGCTTATGCC
TGGAGGAAAGGAGCTTTGGA

Downstream 100 bases:

>100_bases
AGAATGAGGAACGAAGAATTATGCAAGAAATACAATTTATAGTCCCTGCAGCGTTACACGACGAAATGCTGCGCTTGCGA
AATGAAAAACAGATGGACTT

Product: NADH dehydrogenase subunit B

Products: NA

Alternate protein names: NADH dehydrogenase I subunit B; NDH-1 subunit B

Number of amino acids: Translated: 197; Mature: 197

Protein sequence:

>197_residues
MEITKKPKIKSIPYDEFIDNESLEKLVKELNTGGANVFLGVLDDLVNWGRSNSLWPLTFATSCCGIEFMALGAARYDMAR
FGFEVARASPRQADMIMVCGTITNKMAPVLKRLYDQMPDPKYVVAVGGCAVSGGPFKKSYHVLNGVDKILPVDVYIPGCP
PRPEAFYYGMMQLQRKVKIEKFFGGTNRKEKKPEFMK

Sequences:

>Translated_197_residues
MEITKKPKIKSIPYDEFIDNESLEKLVKELNTGGANVFLGVLDDLVNWGRSNSLWPLTFATSCCGIEFMALGAARYDMAR
FGFEVARASPRQADMIMVCGTITNKMAPVLKRLYDQMPDPKYVVAVGGCAVSGGPFKKSYHVLNGVDKILPVDVYIPGCP
PRPEAFYYGMMQLQRKVKIEKFFGGTNRKEKKPEFMK
>Mature_197_residues
MEITKKPKIKSIPYDEFIDNESLEKLVKELNTGGANVFLGVLDDLVNWGRSNSLWPLTFATSCCGIEFMALGAARYDMAR
FGFEVARASPRQADMIMVCGTITNKMAPVLKRLYDQMPDPKYVVAVGGCAVSGGPFKKSYHVLNGVDKILPVDVYIPGCP
PRPEAFYYGMMQLQRKVKIEKFFGGTNRKEKKPEFMK

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be a menaquinone. Couples the redox reaction to proton translo

COG id: COG0377

COG function: function code C; NADH:ubiquinone oxidoreductase 20 kD subunit and related Fe-S oxidoreductases

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein; Cytoplasmic side

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 20 kDa subunit family

Homologues:

Organism=Homo sapiens, GI187281616, Length=140, Percent_Identity=60.7142857142857, Blast_Score=194, Evalue=3e-50,
Organism=Escherichia coli, GI1788624, Length=156, Percent_Identity=55.7692307692308, Blast_Score=184, Evalue=4e-48,
Organism=Escherichia coli, GI1789074, Length=106, Percent_Identity=46.2264150943396, Blast_Score=98, Evalue=4e-22,
Organism=Escherichia coli, GI1788834, Length=121, Percent_Identity=40.495867768595, Blast_Score=93, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI17509685, Length=147, Percent_Identity=53.7414965986395, Blast_Score=181, Evalue=2e-46,
Organism=Drosophila melanogaster, GI18859983, Length=155, Percent_Identity=56.1290322580645, Blast_Score=194, Evalue=4e-50,
Organism=Drosophila melanogaster, GI24642371, Length=155, Percent_Identity=56.1290322580645, Blast_Score=194, Evalue=4e-50,
Organism=Drosophila melanogaster, GI24651058, Length=137, Percent_Identity=59.1240875912409, Blast_Score=187, Evalue=3e-48,

Paralogues:

None

Copy number: 520 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NUOB_BACTN (Q8A0F5)

Other databases:

- EMBL:   AE015928
- RefSeq:   NP_812977.1
- ProteinModelPortal:   Q8A0F5
- SMR:   Q8A0F5
- GeneID:   1073400
- GenomeReviews:   AE015928_GR
- KEGG:   bth:BT_4066
- NMPDR:   fig|226186.1.peg.4064
- HOGENOM:   HBG553221
- OMA:   CCAIEFM
- PhylomeDB:   Q8A0F5
- ProtClustDB:   PRK14816
- BioCyc:   BTHE226186:BT_4066-MONOMER
- GO:   GO:0006810
- HAMAP:   MF_01356
- InterPro:   IPR006137
- InterPro:   IPR006138
- InterPro:   IPR014406
- PANTHER:   PTHR11995:SF2
- PANTHER:   PTHR11995
- TIGRFAMs:   TIGR01957

Pfam domain/function: PF01058 Oxidored_q6

EC number: =1.6.99.5

Molecular weight: Translated: 22077; Mature: 22077

Theoretical pI: Translated: 9.33; Mature: 9.33

Prosite motif: PS01150 COMPLEX1_20K

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
5.1 %Met     (Translated Protein)
7.6 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
5.1 %Met     (Mature Protein)
7.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEITKKPKIKSIPYDEFIDNESLEKLVKELNTGGANVFLGVLDDLVNWGRSNSLWPLTFA
CCCCCCCCCCCCCCHHHCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCHHHH
TSCCGIEFMALGAARYDMARFGFEVARASPRQADMIMVCGTITNKMAPVLKRLYDQMPDP
HHHHCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECHHHHHHHHHHHHHHHHCCCC
KYVVAVGGCAVSGGPFKKSYHVLNGVDKILPVDVYIPGCPPRPEAFYYGMMQLQRKVKIE
CEEEEECCEEECCCCCHHHHHHHHCHHHHEEEEEEECCCCCCCHHHHHHHHHHHHHHHHH
KFFGGTNRKEKKPEFMK
HHHCCCCCCCCCCCCCC
>Mature Secondary Structure
MEITKKPKIKSIPYDEFIDNESLEKLVKELNTGGANVFLGVLDDLVNWGRSNSLWPLTFA
CCCCCCCCCCCCCCHHHCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCHHHH
TSCCGIEFMALGAARYDMARFGFEVARASPRQADMIMVCGTITNKMAPVLKRLYDQMPDP
HHHHCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECHHHHHHHHHHHHHHHHCCCC
KYVVAVGGCAVSGGPFKKSYHVLNGVDKILPVDVYIPGCPPRPEAFYYGMMQLQRKVKIE
CEEEEECCEEECCCCCHHHHHHHHCHHHHEEEEEEECCCCCCCHHHHHHHHHHHHHHHHH
KFFGGTNRKEKKPEFMK
HHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 12663928