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The map label for this gene is nuoN [H]

Identifier: 29349466

GI number: 29349466

Start: 5290993

End: 5292435

Strand: Reverse

Name: nuoN [H]

Synonym: BT_4058

Alternate gene names: 29349466

Gene position: 5292435-5290993 (Counterclockwise)

Preceding gene: 29349467

Following gene: 29349459

Centisome position: 84.54

GC content: 46.78

Gene sequence:

>1443_bases
ATGGATTATTCACAATTTCTACATATGCGGGAAGAGCTGTCGCTCGTAGCAGTTTTACTATTGCTGTTCCTTGCCGATCT
CTTCATGAGTCCGGACGCACACAAGCAAAAAGGGACGAGACCCGTTTTGAACACCATGCTGCCAGTTATCCTGATGGCCA
TCCATACAGCGATCAATCTGGTTCCAGGCACAGCTGCCGACATTTTTGGCGGTATGTATCACTATGTACCCATGCACACG
GTTGTCAAATCAATCCTGAACATAGGCACGCTGATTGTTTTCCTGATGGCACACGAATGGATGAAACGCGACGATACTTC
ATTCAAGCAAGGTGAGTTCTATGTACTTACACTCTCCACCCTGTTCGGTATGTATCTCATGATTTCCGCCGGACACTTCC
TGATGTTCTTCATCGGACTGGAGACAGCTTCCATCCCGATGGCAGCCTTGATCGCTTTCGACAAATACCGCCATAACTCC
GCTGAAGCCGGTGCCAAGTATATCCTTACCGCACTTTTCTCCAGCGCACTGTTACTGTTTGGTCTGTCAATGATTTACGG
TTCCGCAGGAACACTGTATTTCGATGATCTTCCTGCTCATATCGATGGAAATCCGTTGCAAATCATGGCTTTTGTATTCT
TCTTTACCGGTATGGCGTTCAAGCTCTCTCTGGTTCCGTTCCATTTATGGACGGCAGACGTTTACGAAGGTGCACCGAGT
ACGGTTACCGCCTATCTGAGTGTTATCTCCAAAGGTTCGGCAGCATTTGTGCTGATGGCAATTCTGATCAAAGTATTCGC
ACCGATGATACATGACTGGCAAGAAGTACTTTACTGGGTGACTATCGCTTCTATCACCATCGCCAATATATTCGCTATCC
GCCAGCAGAATCTGAAGCGTCTGATGGCGTTCTCCAGTATTTCGCAGGCAGGATATATCATGCTGGGTGTAATCGGAGGA
ACGGCACAGGGAATGACCGCCATGGTATATTACATACTTGTATATGCCGCTGCCAATCTCGGAGTATTTGCCGTCATCAC
TATCGTAGCATTACGCAGTCAGAAGTTTACCCTCGAAGATTATGCGGGACTCTACAAAACGAACCCGAAAATGGCTTTTC
TGATGACTATTTCGCTGTTCTCACTGGCAGGTATTCCTCCGTTTGCCGGATTCTTCTCGAAGTTCTTTATTTTCATGGCA
GCTTTCGAAGCGGGCTTCCACTTGCTGGTATTCATTGCACTGGTTAATACCGTGATTTCATTGTACTACTATCTGCTGAT
TGTAAAAGCAATGTATATCATGCCTTCCGATAACCCGATTCCGACTTTCCGCAGCGACCGCTGTACAAAGTGGGGACTGG
CGCTCTGCACACTGGGCATCATCGGACTGGGTATTGCAAGTATCGTATATCAGTCTATCGATAAACTATCATTCGGAATA
TAA

Upstream 100 bases:

>100_bases
TTCTGGGTAAGCCACATGATTGGTGAAAGTGTACTCCCTGTAGTCTCACAACTCATCCCATAACCTTTATTTTTTAATCT
TTAATTCTTAATTTATAAAG

Downstream 100 bases:

>100_bases
GAAACCGACTCTTTGGGGCTGACTTTTTAGTCAGCCCCATCATTCTTGTCTATTCTTCCGACATCACCACAAAACAGGGA
AGGCTAAACCAAAAACGCGA

Product: NADH dehydrogenase I subunit N

Products: NA

Alternate protein names: NADH dehydrogenase I subunit N; NDH-1 subunit N [H]

Number of amino acids: Translated: 480; Mature: 480

Protein sequence:

>480_residues
MDYSQFLHMREELSLVAVLLLLFLADLFMSPDAHKQKGTRPVLNTMLPVILMAIHTAINLVPGTAADIFGGMYHYVPMHT
VVKSILNIGTLIVFLMAHEWMKRDDTSFKQGEFYVLTLSTLFGMYLMISAGHFLMFFIGLETASIPMAALIAFDKYRHNS
AEAGAKYILTALFSSALLLFGLSMIYGSAGTLYFDDLPAHIDGNPLQIMAFVFFFTGMAFKLSLVPFHLWTADVYEGAPS
TVTAYLSVISKGSAAFVLMAILIKVFAPMIHDWQEVLYWVTIASITIANIFAIRQQNLKRLMAFSSISQAGYIMLGVIGG
TAQGMTAMVYYILVYAAANLGVFAVITIVALRSQKFTLEDYAGLYKTNPKMAFLMTISLFSLAGIPPFAGFFSKFFIFMA
AFEAGFHLLVFIALVNTVISLYYYLLIVKAMYIMPSDNPIPTFRSDRCTKWGLALCTLGIIGLGIASIVYQSIDKLSFGI

Sequences:

>Translated_480_residues
MDYSQFLHMREELSLVAVLLLLFLADLFMSPDAHKQKGTRPVLNTMLPVILMAIHTAINLVPGTAADIFGGMYHYVPMHT
VVKSILNIGTLIVFLMAHEWMKRDDTSFKQGEFYVLTLSTLFGMYLMISAGHFLMFFIGLETASIPMAALIAFDKYRHNS
AEAGAKYILTALFSSALLLFGLSMIYGSAGTLYFDDLPAHIDGNPLQIMAFVFFFTGMAFKLSLVPFHLWTADVYEGAPS
TVTAYLSVISKGSAAFVLMAILIKVFAPMIHDWQEVLYWVTIASITIANIFAIRQQNLKRLMAFSSISQAGYIMLGVIGG
TAQGMTAMVYYILVYAAANLGVFAVITIVALRSQKFTLEDYAGLYKTNPKMAFLMTISLFSLAGIPPFAGFFSKFFIFMA
AFEAGFHLLVFIALVNTVISLYYYLLIVKAMYIMPSDNPIPTFRSDRCTKWGLALCTLGIIGLGIASIVYQSIDKLSFGI
>Mature_480_residues
MDYSQFLHMREELSLVAVLLLLFLADLFMSPDAHKQKGTRPVLNTMLPVILMAIHTAINLVPGTAADIFGGMYHYVPMHT
VVKSILNIGTLIVFLMAHEWMKRDDTSFKQGEFYVLTLSTLFGMYLMISAGHFLMFFIGLETASIPMAALIAFDKYRHNS
AEAGAKYILTALFSSALLLFGLSMIYGSAGTLYFDDLPAHIDGNPLQIMAFVFFFTGMAFKLSLVPFHLWTADVYEGAPS
TVTAYLSVISKGSAAFVLMAILIKVFAPMIHDWQEVLYWVTIASITIANIFAIRQQNLKRLMAFSSISQAGYIMLGVIGG
TAQGMTAMVYYILVYAAANLGVFAVITIVALRSQKFTLEDYAGLYKTNPKMAFLMTISLFSLAGIPPFAGFFSKFFIFMA
AFEAGFHLLVFIALVNTVISLYYYLLIVKAMYIMPSDNPIPTFRSDRCTKWGLALCTLGIIGLGIASIVYQSIDKLSFGI

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be a menaquinone. Couples the redox reaction to proton translo

COG id: COG1007

COG function: function code C; NADH:ubiquinone oxidoreductase subunit 2 (chain N)

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I subunit 2 family [H]

Homologues:

Organism=Escherichia coli, GI145693160, Length=367, Percent_Identity=33.7874659400545, Blast_Score=189, Evalue=3e-49,
Organism=Escherichia coli, GI1788831, Length=318, Percent_Identity=27.3584905660377, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1788614, Length=360, Percent_Identity=26.3888888888889, Blast_Score=78, Evalue=1e-15,
Organism=Escherichia coli, GI1788613, Length=307, Percent_Identity=22.4755700325733, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI1788829, Length=192, Percent_Identity=28.6458333333333, Blast_Score=65, Evalue=1e-11,
Organism=Escherichia coli, GI1788827, Length=267, Percent_Identity=23.5955056179775, Blast_Score=64, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010096
- InterPro:   IPR001750 [H]

Pfam domain/function: PF00361 Oxidored_q1 [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 53140; Mature: 53140

Theoretical pI: Translated: 8.18; Mature: 8.18

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
5.6 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
5.6 %Met     (Mature Protein)
6.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDYSQFLHMREELSLVAVLLLLFLADLFMSPDAHKQKGTRPVLNTMLPVILMAIHTAINL
CCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHCCCCHHHHHHHHHHHHHHHHHHHHC
VPGTAADIFGGMYHYVPMHTVVKSILNIGTLIVFLMAHEWMKRDDTSFKQGEFYVLTLST
CCCCHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEHHH
LFGMYLMISAGHFLMFFIGLETASIPMAALIAFDKYRHNSAEAGAKYILTALFSSALLLF
HHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHH
GLSMIYGSAGTLYFDDLPAHIDGNPLQIMAFVFFFTGMAFKLSLVPFHLWTADVYEGAPS
HHHHHHCCCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCH
TVTAYLSVISKGSAAFVLMAILIKVFAPMIHDWQEVLYWVTIASITIANIFAIRQQNLKR
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LMAFSSISQAGYIMLGVIGGTAQGMTAMVYYILVYAAANLGVFAVITIVALRSQKFTLED
HHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCHHH
YAGLYKTNPKMAFLMTISLFSLAGIPPFAGFFSKFFIFMAAFEAGFHLLVFIALVNTVIS
HHHHHCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LYYYLLIVKAMYIMPSDNPIPTFRSDRCTKWGLALCTLGIIGLGIASIVYQSIDKLSFGI
HHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MDYSQFLHMREELSLVAVLLLLFLADLFMSPDAHKQKGTRPVLNTMLPVILMAIHTAINL
CCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHCCCCHHHHHHHHHHHHHHHHHHHHC
VPGTAADIFGGMYHYVPMHTVVKSILNIGTLIVFLMAHEWMKRDDTSFKQGEFYVLTLST
CCCCHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEHHH
LFGMYLMISAGHFLMFFIGLETASIPMAALIAFDKYRHNSAEAGAKYILTALFSSALLLF
HHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHH
GLSMIYGSAGTLYFDDLPAHIDGNPLQIMAFVFFFTGMAFKLSLVPFHLWTADVYEGAPS
HHHHHHCCCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCH
TVTAYLSVISKGSAAFVLMAILIKVFAPMIHDWQEVLYWVTIASITIANIFAIRQQNLKR
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LMAFSSISQAGYIMLGVIGGTAQGMTAMVYYILVYAAANLGVFAVITIVALRSQKFTLED
HHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCHHH
YAGLYKTNPKMAFLMTISLFSLAGIPPFAGFFSKFFIFMAAFEAGFHLLVFIALVNTVIS
HHHHHCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LYYYLLIVKAMYIMPSDNPIPTFRSDRCTKWGLALCTLGIIGLGIASIVYQSIDKLSFGI
HHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA