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The map label for this gene is ndhD2 [H]

Identifier: 29349467

GI number: 29349467

Start: 5292473

End: 5293957

Strand: Reverse

Name: ndhD2 [H]

Synonym: BT_4059

Alternate gene names: 29349467

Gene position: 5293957-5292473 (Counterclockwise)

Preceding gene: 29349468

Following gene: 29349466

Centisome position: 84.56

GC content: 49.23

Gene sequence:

>1485_bases
ATGAATTTTTTATCAATCTTCGTACTCATCCCCTTACTGATGCTTGCCGGACTTTGGGCAGCACGGGGAATCAAAGCGAT
TCGGGGGGTTATGGTTACCGGCGCATCGGCACTTCTGATTGCCTCCGTCGTACTTACCTTTATGTATTTGGGAGAACGCA
GTGCCGGCAACACGGCAGAAATGCTATTCCGTGCGGATACACTCTGGTATGCACCGCTTCACATCTCCTACTCGGTAGGC
GTTGACGGTATTTCGGTAGCCATGTTGTTATTGTCTGCCGTTATTGTATTCACAGGTACTTTCGCCTCCTGGCGTCTGCA
GCCTCTGACAAAAGAATATTTCCTTTGGTTCACACTGTTGTCTATGGGAGTCTTCGGATTCTTTATATCCATCGACTTAT
TCACCATGTTCATGTTCTACGAAATCGCGCTGATTCCTATGTACCTGCTGATCGGTGTATGGGGTTCGGGACGTAAAGAA
TATGCAGCCATGAAGCTGACCCTTATGCTGATGGGCGGTTCCGCTTTCCTGCTGATCGGTATTCTCGGAATCTATTTCGG
TTCGGGAGCTACCACCATGAACCTGCTGGAAATAGCACAGCTACATAATATTCCTTTTGCACAGCAATGTATCTGGTTCC
CGCTTACCTTCCTCGGGTTCGGTGTGCTGGGTGCTCTTTTCCCGTTCCACACATGGAGTCCCGATGGTCACGCTTCCGCC
CCGACAGCTGTATCCATGCTTCACGCCGGAGTACTGATGAAACTGGGAGGTTACGGATGTTTCCGTATCGCCATGTATCT
GATGCCGGAAGCCGCTAACGAACTTTCATGGATATTCCTGATACTGACAGGTATCTCCGTTGTTTACGGAGCATTCTCCG
CTTGCGTACAGACAGACTTGAAATACATCAACGCTTACTCTTCCGTTTCTCACTGCGGACTGGTACTTTTCGCCATTCTG
ATGCTGAATCAGACTGCTGCGACAGGAGCCATTCTTCAGATGCTTTCCCACGGATTGATGACAGCCTTGTTCTTTGCCCT
GATCGGTATGATTTATGGACGTACACACACACGTGATGTCCGCGAGCTTACCGGATTAATGAAAGTAATGCCGTTCCTCA
GCGTATGTTATGTGATTGCCGGTCTTGCCAATCTCGGTTTGCCGGGATTAAGCGGGTTCATTGCCGAAATGACTATCTTC
GTAGGTTCTTTCCAGAACAACGACGTATTCCACCGTACACTGACCATCATCGCTTGTTCATCCATCGTGATCACGGCAGT
CTATATCCTGCGTCTGGTAGGCAAGATTCTGTACGGTACCTGCACCAACAAACATCATCTTGCGCTGACAGACGCGACCT
GGGATGAACGGGTAGCTGTTATCTGCCTCATCGTTTGTGTAGCCGGACTGGGTATGGCTCCTTTCTGGGTAAGCCACATG
ATTGGTGAAAGTGTACTCCCTGTAGTCTCACAACTCATCCCATAA

Upstream 100 bases:

>100_bases
CTGCTGTTAGTTCTATAGCAAGTATTAACTATCAGGTATTAGATATTAAGCTGCGCAACAAGTCGCTGCGAATTAATACT
TAAAACTTAATAATTCAATT

Downstream 100 bases:

>100_bases
CCTTTATTTTTTAATCTTTAATTCTTAATTTATAAAGATGGATTATTCACAATTTCTACATATGCGGGAAGAGCTGTCGC
TCGTAGCAGTTTTACTATTG

Product: NADH dehydrogenase I subunit M

Products: NAD+; ubiquinol

Alternate protein names: NAD(P)H dehydrogenase I, subunit D-2; NDH-1, chain 4-2 [H]

Number of amino acids: Translated: 494; Mature: 494

Protein sequence:

>494_residues
MNFLSIFVLIPLLMLAGLWAARGIKAIRGVMVTGASALLIASVVLTFMYLGERSAGNTAEMLFRADTLWYAPLHISYSVG
VDGISVAMLLLSAVIVFTGTFASWRLQPLTKEYFLWFTLLSMGVFGFFISIDLFTMFMFYEIALIPMYLLIGVWGSGRKE
YAAMKLTLMLMGGSAFLLIGILGIYFGSGATTMNLLEIAQLHNIPFAQQCIWFPLTFLGFGVLGALFPFHTWSPDGHASA
PTAVSMLHAGVLMKLGGYGCFRIAMYLMPEAANELSWIFLILTGISVVYGAFSACVQTDLKYINAYSSVSHCGLVLFAIL
MLNQTAATGAILQMLSHGLMTALFFALIGMIYGRTHTRDVRELTGLMKVMPFLSVCYVIAGLANLGLPGLSGFIAEMTIF
VGSFQNNDVFHRTLTIIACSSIVITAVYILRLVGKILYGTCTNKHHLALTDATWDERVAVICLIVCVAGLGMAPFWVSHM
IGESVLPVVSQLIP

Sequences:

>Translated_494_residues
MNFLSIFVLIPLLMLAGLWAARGIKAIRGVMVTGASALLIASVVLTFMYLGERSAGNTAEMLFRADTLWYAPLHISYSVG
VDGISVAMLLLSAVIVFTGTFASWRLQPLTKEYFLWFTLLSMGVFGFFISIDLFTMFMFYEIALIPMYLLIGVWGSGRKE
YAAMKLTLMLMGGSAFLLIGILGIYFGSGATTMNLLEIAQLHNIPFAQQCIWFPLTFLGFGVLGALFPFHTWSPDGHASA
PTAVSMLHAGVLMKLGGYGCFRIAMYLMPEAANELSWIFLILTGISVVYGAFSACVQTDLKYINAYSSVSHCGLVLFAIL
MLNQTAATGAILQMLSHGLMTALFFALIGMIYGRTHTRDVRELTGLMKVMPFLSVCYVIAGLANLGLPGLSGFIAEMTIF
VGSFQNNDVFHRTLTIIACSSIVITAVYILRLVGKILYGTCTNKHHLALTDATWDERVAVICLIVCVAGLGMAPFWVSHM
IGESVLPVVSQLIP
>Mature_494_residues
MNFLSIFVLIPLLMLAGLWAARGIKAIRGVMVTGASALLIASVVLTFMYLGERSAGNTAEMLFRADTLWYAPLHISYSVG
VDGISVAMLLLSAVIVFTGTFASWRLQPLTKEYFLWFTLLSMGVFGFFISIDLFTMFMFYEIALIPMYLLIGVWGSGRKE
YAAMKLTLMLMGGSAFLLIGILGIYFGSGATTMNLLEIAQLHNIPFAQQCIWFPLTFLGFGVLGALFPFHTWSPDGHASA
PTAVSMLHAGVLMKLGGYGCFRIAMYLMPEAANELSWIFLILTGISVVYGAFSACVQTDLKYINAYSSVSHCGLVLFAIL
MLNQTAATGAILQMLSHGLMTALFFALIGMIYGRTHTRDVRELTGLMKVMPFLSVCYVIAGLANLGLPGLSGFIAEMTIF
VGSFQNNDVFHRTLTIIACSSIVITAVYILRLVGKILYGTCTNKHHLALTDATWDERVAVICLIVCVAGLGMAPFWVSHM
IGESVLPVVSQLIP

Specific function: NDH-1 shuttles electrons from NAD(P)H, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be plastoquinone. Couples the redox reaction to proton tran

COG id: COG1008

COG function: function code C; NADH:ubiquinone oxidoreductase subunit 4 (chain M)

Gene ontology:

Cell location: Cellular thylakoid membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I subunit 4 family [H]

Homologues:

Organism=Homo sapiens, GI251831116, Length=345, Percent_Identity=31.8840579710145, Blast_Score=135, Evalue=1e-31,
Organism=Homo sapiens, GI251831117, Length=219, Percent_Identity=29.6803652968037, Blast_Score=77, Evalue=3e-14,
Organism=Escherichia coli, GI1788613, Length=394, Percent_Identity=35.2791878172589, Blast_Score=208, Evalue=6e-55,
Organism=Escherichia coli, GI1788614, Length=469, Percent_Identity=27.5053304904051, Blast_Score=116, Evalue=4e-27,
Organism=Escherichia coli, GI1788827, Length=228, Percent_Identity=32.0175438596491, Blast_Score=112, Evalue=8e-26,
Organism=Escherichia coli, GI1788831, Length=317, Percent_Identity=29.9684542586751, Blast_Score=110, Evalue=2e-25,
Organism=Escherichia coli, GI1788829, Length=299, Percent_Identity=24.7491638795987, Blast_Score=83, Evalue=4e-17,
Organism=Escherichia coli, GI2367154, Length=318, Percent_Identity=26.1006289308176, Blast_Score=80, Evalue=3e-16,
Organism=Escherichia coli, GI145693160, Length=310, Percent_Identity=26.1290322580645, Blast_Score=70, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022997
- InterPro:   IPR010227
- InterPro:   IPR001750
- InterPro:   IPR003918 [H]

Pfam domain/function: PF00361 Oxidored_q1 [H]

EC number: 1.6.5.3

Molecular weight: Translated: 53821; Mature: 53821

Theoretical pI: Translated: 7.77; Mature: 7.77

Prosite motif: PS00338 SOMATOTROPIN_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
5.5 %Met     (Translated Protein)
7.3 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
5.5 %Met     (Mature Protein)
7.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNFLSIFVLIPLLMLAGLWAARGIKAIRGVMVTGASALLIASVVLTFMYLGERSAGNTAE
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCHHH
MLFRADTLWYAPLHISYSVGVDGISVAMLLLSAVIVFTGTFASWRLQPLTKEYFLWFTLL
HHHHHHHEEEEEEEEEEECCCCHHHHHHHHHHHHHHHHCCHHHCCCCHHHHHHHHHHHHH
SMGVFGFFISIDLFTMFMFYEIALIPMYLLIGVWGSGRKEYAAMKLTLMLMGGSAFLLIG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHEECCHHHHHHH
ILGIYFGSGATTMNLLEIAQLHNIPFAQQCIWFPLTFLGFGVLGALFPFHTWSPDGHASA
HHHHHHCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
PTAVSMLHAGVLMKLGGYGCFRIAMYLMPEAANELSWIFLILTGISVVYGAFSACVQTDL
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KYINAYSSVSHCGLVLFAILMLNQTAATGAILQMLSHGLMTALFFALIGMIYGRTHTRDV
HHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHH
RELTGLMKVMPFLSVCYVIAGLANLGLPGLSGFIAEMTIFVGSFQNNDVFHRTLTIIACS
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHH
SIVITAVYILRLVGKILYGTCTNKHHLALTDATWDERVAVICLIVCVAGLGMAPFWVSHM
HHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHHHHHHHCCHHHHHHHHH
IGESVLPVVSQLIP
HHHHHHHHHHHHCC
>Mature Secondary Structure
MNFLSIFVLIPLLMLAGLWAARGIKAIRGVMVTGASALLIASVVLTFMYLGERSAGNTAE
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCHHH
MLFRADTLWYAPLHISYSVGVDGISVAMLLLSAVIVFTGTFASWRLQPLTKEYFLWFTLL
HHHHHHHEEEEEEEEEEECCCCHHHHHHHHHHHHHHHHCCHHHCCCCHHHHHHHHHHHHH
SMGVFGFFISIDLFTMFMFYEIALIPMYLLIGVWGSGRKEYAAMKLTLMLMGGSAFLLIG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHEECCHHHHHHH
ILGIYFGSGATTMNLLEIAQLHNIPFAQQCIWFPLTFLGFGVLGALFPFHTWSPDGHASA
HHHHHHCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
PTAVSMLHAGVLMKLGGYGCFRIAMYLMPEAANELSWIFLILTGISVVYGAFSACVQTDL
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KYINAYSSVSHCGLVLFAILMLNQTAATGAILQMLSHGLMTALFFALIGMIYGRTHTRDV
HHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHH
RELTGLMKVMPFLSVCYVIAGLANLGLPGLSGFIAEMTIFVGSFQNNDVFHRTLTIIACS
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHH
SIVITAVYILRLVGKILYGTCTNKHHLALTDATWDERVAVICLIVCVAGLGMAPFWVSHM
HHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHHHHHHHCCHHHHHHHHH
IGESVLPVVSQLIP
HHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADH; H+; ubiquinone

Specific reaction: NADH + H+ + ubiquinone = NAD+ + ubiquinol

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7846157; 8905231 [H]