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The map label for this gene is alaS [H]

Identifier: 29349403

GI number: 29349403

Start: 5205400

End: 5208018

Strand: Reverse

Name: alaS [H]

Synonym: BT_3995

Alternate gene names: 29349403

Gene position: 5208018-5205400 (Counterclockwise)

Preceding gene: 29349406

Following gene: 29349402

Centisome position: 83.19

GC content: 48.87

Gene sequence:

>2619_bases
ATGTTGACTGCAAAAGAGATCAGAGATTCATTCAAGAATTTCTTTGAGTCGAAAGGACACCACATTGTTCCCTCGGCTCC
GATGGTGATAAAAGATGACCCTACCCTGATGTTTACCAACGCAGGGATGAACCAGTTTAAAGATATTATTTTGGGTAACC
ACCCGGCCAAATATCACAGAGTCGCGGACTCTCAGAAATGTCTGCGTGTAAGCGGAAAGCATAATGACCTGGAAGAAGTG
GGACACGATACGTACCACCACACCATGTTCGAAATGCTGGGCAACTGGTCATTCGGTGACTACTTCAAGAAAGAAGCCAT
CAACTGGGCATGGGAATATCTGGTAGAAGTATTGAAACTGAATCCGGAACACCTGTACGCCACTGTATTCGAAGGAAGTC
CCGAAGAAGGACTGAGCCGTGACGACGAAGCCGCTTCTTACTGGGAACAGTATCTGCCGAAAGATCACATCATCAACGGC
AACAAGCACGACAACTTCTGGGAAATGGGTGATACAGGTCCTTGCGGACCTTGTTCGGAAATCCACATCGACCTCCGTCC
GGCAGAAGAACGTGCCAAAATCTCCGGCCGTGATCTCGTCAACCACGATCATCCCCAAGTGATTGAAATATGGAACCTTG
TGTTCATGCAATATAACCGTAAAGCTGACGGCAGCCTCGAACCACTTCCTGCAAAGGTTATCGATACAGGTATGGGATTC
GAACGCCTCTGTATGGCTTTGCAGGGCAAGACTTCCAACTACGATACAGACGTATTCCAGCCGATGCTGAAAGCGATTGC
AGCAATGTCAGGCACAGAGTACGGAAAAGACAAACAACAGGACATCGCCATGCGTGTAATCGCTGACCACATCCGTACGA
TTGCTTTCTCCATTACGGACGGTCAGCTGCCTTCCAATGCCAAGGCAGGTTATGTAATCCGCCGTATCCTCCGCCGCGCC
GTTCGCTACGGATATACTTTCCTCGGACAGAAACAGTCATTCATGTACAAGCTGCTTCCGGTGTTGATCGACAACATGGG
AGACGCTTATCCGGAACTGATCGCACAGAAAGGTCTGATCGAAAAAGTAATCAAGGAAGAAGAAGAAGCCTTCCTTCGCA
CGCTGGAAACAGGTATCCGTCTGCTGGATAAAACAATGGGAGATACAAAGGCCGCCGGAAAGACAGAAATCAGCGGTAAA
GATGCCTTTACCTTATATGATACTTTCGGTTTTCCGTTAGACCTGACAGAACTGATTCTTCGCGAAAACGGAATGACAGT
CAACATCGAAGAGTTCAACGCGGAAATGCAACAGCAGAAACAGCGTGCACGTAACGCTGCCGCCATCGAAACCGGTGACT
GGGTGACTCTGAGAGAAGGAACAACCGAATTCGTAGGTTACGACTATACTGAATACGAAGCATCTATTCTCCGCTATCGT
CAGATCAAGCAGAAAAACCAGACACTGTATCAGATCGTACTTGACTGTACTCCGTTCTATGCAGAAAGCGGTGGTCAGGT
AGGCGACACCGGTGTATTGGTCAGCGAATTCGAGACCATCGAAGTGATTGACACCAAGAAAGAGAACAATCTACCGATAC
ATATTACCAAAAAGTTGCCGGAACATCCGGAAGCTCCGATGATGGCTTGTGTAGACACCGACAAACGGGCAGCCTGCGCA
GCCAATCACTCGGCAACTCACCTGCTGGACTCTGCCCTGCGTGAAGTACTGGGCGAGCATATCGAACAGAAAGGTTCGTT
AGTAACACCGGATTCACTGCGTTTCGACTTCTCTCACTTCCAGAAGGTGACCGACGAAGAAATCCGCCAGGTAGAACACC
TGGTGAATGCCAAGATTCGTGCCAACATACCTTTGAAGGAATACCGCAACATTCCTATCGAAGAAGCGAAAGAGCTAGGA
GCTATCGCCCTCTTCGGTGAAAAGTACGGTGAAAGAGTACGTGTCATCCAGTTCGGTTCTTCTATCGAATTCTGTGGAGG
TATCCACGTAGCCGCAACCGGAAATATCGGTATGGTGAAGATCATTTCCGAAAGCTCTGTTGCAGCCGGTGTACGCCGTA
TCGAAGCATATACGGGAGCCCGTGTGGAAGAAATGCTGGATACCATTCAGGATACGATCAGTGAATTGAAATCGCTCTTC
AACAACGCACCCGACTTGGGTATTGCCATCCGCAAGTATATTGAGGAAAACGCAGGACTGAAAAAGCAGGTAGAAGACTA
CATGAAAGAGAAAGAAGCCTCGCTGAAAGAAAGACTGCTGAAGAATATACAGGAAATTCACGGTATCAAGGTAATCAAGT
TCTGTGCTCCGTTGCCGGCGGAAGTGGTGAAGAATATCGCCTTCCAGCTTCGCGGTGAAATCACAGAAAACCTGTTCTTT
GTGGCCGGAAGCCTCGACAACGGCAAGCCTATGCTGACCGTCATGCTAAGTGACAATCTGGTGGCCGGTGGCTTGAAAGC
AGGCAACCTGGTGAAAGAAGCAGCCAAACTGATTCAAGGCGGCGGAGGCGGTCAACCTCATTTCGCTACTGCCGGAGGCA
AGAATACAGACGGACTGAATGCCGCTATCGAAAAGGTACTGGAACTCGCAGGTATCTAA

Upstream 100 bases:

>100_bases
GAAATATTTCGCTTGTTAATACGGCAAAAGTAATAAAAACAAGCTATCTTTGCACAGTTTTTTGAGAATATTAACCTCAA
CAATAGATATATAGAAGAAT

Downstream 100 bases:

>100_bases
AAAAGCAGTAACATACATCCAATACAAGAACCGTGTCTTAAAGTCACCCGCACTTTAAGGCACGTTTTTTTATTTCAGAT
TCCAGTGTCAACAGCAACTA

Product: alanyl-tRNA synthetase

Products: NA

Alternate protein names: Alanine--tRNA ligase; AlaRS [H]

Number of amino acids: Translated: 872; Mature: 872

Protein sequence:

>872_residues
MLTAKEIRDSFKNFFESKGHHIVPSAPMVIKDDPTLMFTNAGMNQFKDIILGNHPAKYHRVADSQKCLRVSGKHNDLEEV
GHDTYHHTMFEMLGNWSFGDYFKKEAINWAWEYLVEVLKLNPEHLYATVFEGSPEEGLSRDDEAASYWEQYLPKDHIING
NKHDNFWEMGDTGPCGPCSEIHIDLRPAEERAKISGRDLVNHDHPQVIEIWNLVFMQYNRKADGSLEPLPAKVIDTGMGF
ERLCMALQGKTSNYDTDVFQPMLKAIAAMSGTEYGKDKQQDIAMRVIADHIRTIAFSITDGQLPSNAKAGYVIRRILRRA
VRYGYTFLGQKQSFMYKLLPVLIDNMGDAYPELIAQKGLIEKVIKEEEEAFLRTLETGIRLLDKTMGDTKAAGKTEISGK
DAFTLYDTFGFPLDLTELILRENGMTVNIEEFNAEMQQQKQRARNAAAIETGDWVTLREGTTEFVGYDYTEYEASILRYR
QIKQKNQTLYQIVLDCTPFYAESGGQVGDTGVLVSEFETIEVIDTKKENNLPIHITKKLPEHPEAPMMACVDTDKRAACA
ANHSATHLLDSALREVLGEHIEQKGSLVTPDSLRFDFSHFQKVTDEEIRQVEHLVNAKIRANIPLKEYRNIPIEEAKELG
AIALFGEKYGERVRVIQFGSSIEFCGGIHVAATGNIGMVKIISESSVAAGVRRIEAYTGARVEEMLDTIQDTISELKSLF
NNAPDLGIAIRKYIEENAGLKKQVEDYMKEKEASLKERLLKNIQEIHGIKVIKFCAPLPAEVVKNIAFQLRGEITENLFF
VAGSLDNGKPMLTVMLSDNLVAGGLKAGNLVKEAAKLIQGGGGGQPHFATAGGKNTDGLNAAIEKVLELAGI

Sequences:

>Translated_872_residues
MLTAKEIRDSFKNFFESKGHHIVPSAPMVIKDDPTLMFTNAGMNQFKDIILGNHPAKYHRVADSQKCLRVSGKHNDLEEV
GHDTYHHTMFEMLGNWSFGDYFKKEAINWAWEYLVEVLKLNPEHLYATVFEGSPEEGLSRDDEAASYWEQYLPKDHIING
NKHDNFWEMGDTGPCGPCSEIHIDLRPAEERAKISGRDLVNHDHPQVIEIWNLVFMQYNRKADGSLEPLPAKVIDTGMGF
ERLCMALQGKTSNYDTDVFQPMLKAIAAMSGTEYGKDKQQDIAMRVIADHIRTIAFSITDGQLPSNAKAGYVIRRILRRA
VRYGYTFLGQKQSFMYKLLPVLIDNMGDAYPELIAQKGLIEKVIKEEEEAFLRTLETGIRLLDKTMGDTKAAGKTEISGK
DAFTLYDTFGFPLDLTELILRENGMTVNIEEFNAEMQQQKQRARNAAAIETGDWVTLREGTTEFVGYDYTEYEASILRYR
QIKQKNQTLYQIVLDCTPFYAESGGQVGDTGVLVSEFETIEVIDTKKENNLPIHITKKLPEHPEAPMMACVDTDKRAACA
ANHSATHLLDSALREVLGEHIEQKGSLVTPDSLRFDFSHFQKVTDEEIRQVEHLVNAKIRANIPLKEYRNIPIEEAKELG
AIALFGEKYGERVRVIQFGSSIEFCGGIHVAATGNIGMVKIISESSVAAGVRRIEAYTGARVEEMLDTIQDTISELKSLF
NNAPDLGIAIRKYIEENAGLKKQVEDYMKEKEASLKERLLKNIQEIHGIKVIKFCAPLPAEVVKNIAFQLRGEITENLFF
VAGSLDNGKPMLTVMLSDNLVAGGLKAGNLVKEAAKLIQGGGGGQPHFATAGGKNTDGLNAAIEKVLELAGI
>Mature_872_residues
MLTAKEIRDSFKNFFESKGHHIVPSAPMVIKDDPTLMFTNAGMNQFKDIILGNHPAKYHRVADSQKCLRVSGKHNDLEEV
GHDTYHHTMFEMLGNWSFGDYFKKEAINWAWEYLVEVLKLNPEHLYATVFEGSPEEGLSRDDEAASYWEQYLPKDHIING
NKHDNFWEMGDTGPCGPCSEIHIDLRPAEERAKISGRDLVNHDHPQVIEIWNLVFMQYNRKADGSLEPLPAKVIDTGMGF
ERLCMALQGKTSNYDTDVFQPMLKAIAAMSGTEYGKDKQQDIAMRVIADHIRTIAFSITDGQLPSNAKAGYVIRRILRRA
VRYGYTFLGQKQSFMYKLLPVLIDNMGDAYPELIAQKGLIEKVIKEEEEAFLRTLETGIRLLDKTMGDTKAAGKTEISGK
DAFTLYDTFGFPLDLTELILRENGMTVNIEEFNAEMQQQKQRARNAAAIETGDWVTLREGTTEFVGYDYTEYEASILRYR
QIKQKNQTLYQIVLDCTPFYAESGGQVGDTGVLVSEFETIEVIDTKKENNLPIHITKKLPEHPEAPMMACVDTDKRAACA
ANHSATHLLDSALREVLGEHIEQKGSLVTPDSLRFDFSHFQKVTDEEIRQVEHLVNAKIRANIPLKEYRNIPIEEAKELG
AIALFGEKYGERVRVIQFGSSIEFCGGIHVAATGNIGMVKIISESSVAAGVRRIEAYTGARVEEMLDTIQDTISELKSLF
NNAPDLGIAIRKYIEENAGLKKQVEDYMKEKEASLKERLLKNIQEIHGIKVIKFCAPLPAEVVKNIAFQLRGEITENLFF
VAGSLDNGKPMLTVMLSDNLVAGGLKAGNLVKEAAKLIQGGGGGQPHFATAGGKNTDGLNAAIEKVLELAGI

Specific function: Catalyzes the attachment of alanine to tRNA(Ala) in a two-step reaction:alanine is first activated by ATP to form Ala- AMP and then transferred to the acceptor end of tRNA(Ala). Also edits incorrectly charged Ser-tRNA(Ala) and Gly-tRNA(Ala) via its editin

COG id: COG0013

COG function: function code J; Alanyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI109148542, Length=973, Percent_Identity=37.8211716341213, Blast_Score=568, Evalue=1e-161,
Organism=Homo sapiens, GI38569417, Length=783, Percent_Identity=36.7816091954023, Blast_Score=474, Evalue=1e-133,
Organism=Escherichia coli, GI1789048, Length=885, Percent_Identity=43.2768361581921, Blast_Score=661, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17506981, Length=978, Percent_Identity=36.8098159509202, Blast_Score=564, Evalue=1e-161,
Organism=Caenorhabditis elegans, GI17536681, Length=755, Percent_Identity=36.4238410596026, Blast_Score=425, Evalue=1e-119,
Organism=Saccharomyces cerevisiae, GI6324911, Length=763, Percent_Identity=43.1192660550459, Blast_Score=591, Evalue=1e-169,
Organism=Drosophila melanogaster, GI24582809, Length=974, Percent_Identity=37.4743326488706, Blast_Score=565, Evalue=1e-161,
Organism=Drosophila melanogaster, GI45552267, Length=974, Percent_Identity=37.4743326488706, Blast_Score=565, Evalue=1e-161,
Organism=Drosophila melanogaster, GI24658214, Length=816, Percent_Identity=33.9460784313725, Blast_Score=389, Evalue=1e-108,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002318
- InterPro:   IPR018162
- InterPro:   IPR018165
- InterPro:   IPR018164
- InterPro:   IPR023033
- InterPro:   IPR003156
- InterPro:   IPR018163
- InterPro:   IPR012947 [H]

Pfam domain/function: PF02272 DHHA1; PF01411 tRNA-synt_2c; PF07973 tRNA_SAD [H]

EC number: =6.1.1.7 [H]

Molecular weight: Translated: 97555; Mature: 97555

Theoretical pI: Translated: 5.49; Mature: 5.49

Prosite motif: PS50860 AA_TRNA_LIGASE_II_ALA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTAKEIRDSFKNFFESKGHHIVPSAPMVIKDDPTLMFTNAGMNQFKDIILGNHPAKYHR
CCCHHHHHHHHHHHHHCCCCEECCCCCEEEECCCCEEEECCCHHHHHHHHCCCCCHHHHH
VADSQKCLRVSGKHNDLEEVGHDTYHHTMFEMLGNWSFGDYFKKEAINWAWEYLVEVLKL
HCCCHHHHHCCCCCCCHHHHCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHC
NPEHLYATVFEGSPEEGLSRDDEAASYWEQYLPKDHIINGNKHDNFWEMGDTGPCGPCSE
CCCCEEEEEECCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
IHIDLRPAEERAKISGRDLVNHDHPQVIEIWNLVFMQYNRKADGSLEPLPAKVIDTGMGF
EEEEECCCHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHCCCCH
ERLCMALQGKTSNYDTDVFQPMLKAIAAMSGTEYGKDKQQDIAMRVIADHIRTIAFSITD
HHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHEEEEEEC
GQLPSNAKAGYVIRRILRRAVRYGYTFLGQKQSFMYKLLPVLIDNMGDAYPELIAQKGLI
CCCCCCCCHHHHHHHHHHHHHHHCHHHHCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
EKVIKEEEEAFLRTLETGIRLLDKTMGDTKAAGKTEISGKDAFTLYDTFGFPLDLTELIL
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEECCCCCHHHHHHHH
RENGMTVNIEEFNAEMQQQKQRARNAAAIETGDWVTLREGTTEFVGYDYTEYEASILRYR
HCCCCEEEHHHHHHHHHHHHHHHHCCCEEECCCEEEECCCCCHHCCCCHHHHHHHHHHHH
QIKQKNQTLYQIVLDCTPFYAESGGQVGDTGVLVSEFETIEVIDTKKENNLPIHITKKLP
HHHHHHHHHHHHHHHCCCHHHCCCCCCCCCCEEEECCCEEEEEECCCCCCCCEEEECCCC
EHPEAPMMACVDTDKRAACAANHSATHLLDSALREVLGEHIEQKGSLVTPDSLRFDFSHF
CCCCCCEEEEECCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHH
QKVTDEEIRQVEHLVNAKIRANIPLKEYRNIPIEEAKELGAIALFGEKYGERVRVIQFGS
HHCCHHHHHHHHHHHCCHHEECCCHHHHCCCCHHHHHHCCEEEEECHHHCCEEEEEEECC
SIEFCGGIHVAATGNIGMVKIISESSVAAGVRRIEAYTGARVEEMLDTIQDTISELKSLF
CCCCCCCEEEEEECCCCEEEEECCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHH
NNAPDLGIAIRKYIEENAGLKKQVEDYMKEKEASLKERLLKNIQEIHGIKVIKFCAPLPA
CCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCHH
EVVKNIAFQLRGEITENLFFVAGSLDNGKPMLTVMLSDNLVAGGLKAGNLVKEAAKLIQG
HHHHHHHHHHHHHHHCCEEEEEECCCCCCCEEEEEEECCEEECCCCHHHHHHHHHHHHHC
GGGGQPHFATAGGKNTDGLNAAIEKVLELAGI
CCCCCCCEECCCCCCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure
MLTAKEIRDSFKNFFESKGHHIVPSAPMVIKDDPTLMFTNAGMNQFKDIILGNHPAKYHR
CCCHHHHHHHHHHHHHCCCCEECCCCCEEEECCCCEEEECCCHHHHHHHHCCCCCHHHHH
VADSQKCLRVSGKHNDLEEVGHDTYHHTMFEMLGNWSFGDYFKKEAINWAWEYLVEVLKL
HCCCHHHHHCCCCCCCHHHHCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHC
NPEHLYATVFEGSPEEGLSRDDEAASYWEQYLPKDHIINGNKHDNFWEMGDTGPCGPCSE
CCCCEEEEEECCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
IHIDLRPAEERAKISGRDLVNHDHPQVIEIWNLVFMQYNRKADGSLEPLPAKVIDTGMGF
EEEEECCCHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHCCCCH
ERLCMALQGKTSNYDTDVFQPMLKAIAAMSGTEYGKDKQQDIAMRVIADHIRTIAFSITD
HHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHEEEEEEC
GQLPSNAKAGYVIRRILRRAVRYGYTFLGQKQSFMYKLLPVLIDNMGDAYPELIAQKGLI
CCCCCCCCHHHHHHHHHHHHHHHCHHHHCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
EKVIKEEEEAFLRTLETGIRLLDKTMGDTKAAGKTEISGKDAFTLYDTFGFPLDLTELIL
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEECCCCCHHHHHHHH
RENGMTVNIEEFNAEMQQQKQRARNAAAIETGDWVTLREGTTEFVGYDYTEYEASILRYR
HCCCCEEEHHHHHHHHHHHHHHHHCCCEEECCCEEEECCCCCHHCCCCHHHHHHHHHHHH
QIKQKNQTLYQIVLDCTPFYAESGGQVGDTGVLVSEFETIEVIDTKKENNLPIHITKKLP
HHHHHHHHHHHHHHHCCCHHHCCCCCCCCCCEEEECCCEEEEEECCCCCCCCEEEECCCC
EHPEAPMMACVDTDKRAACAANHSATHLLDSALREVLGEHIEQKGSLVTPDSLRFDFSHF
CCCCCCEEEEECCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHH
QKVTDEEIRQVEHLVNAKIRANIPLKEYRNIPIEEAKELGAIALFGEKYGERVRVIQFGS
HHCCHHHHHHHHHHHCCHHEECCCHHHHCCCCHHHHHHCCEEEEECHHHCCEEEEEEECC
SIEFCGGIHVAATGNIGMVKIISESSVAAGVRRIEAYTGARVEEMLDTIQDTISELKSLF
CCCCCCCEEEEEECCCCEEEEECCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHH
NNAPDLGIAIRKYIEENAGLKKQVEDYMKEKEASLKERLLKNIQEIHGIKVIKFCAPLPA
CCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCHH
EVVKNIAFQLRGEITENLFFVAGSLDNGKPMLTVMLSDNLVAGGLKAGNLVKEAAKLIQG
HHHHHHHHHHHHHHHCCEEEEEECCCCCCCEEEEEEECCEEECCCCHHHHHHHHHHHHHC
GGGGQPHFATAGGKNTDGLNAAIEKVLELAGI
CCCCCCCEECCCCCCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA