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The map label for this gene is spoT [H]

Identifier: 29349406

GI number: 29349406

Start: 5209459

End: 5211702

Strand: Reverse

Name: spoT [H]

Synonym: BT_3998

Alternate gene names: 29349406

Gene position: 5211702-5209459 (Counterclockwise)

Preceding gene: 29349407

Following gene: 29349403

Centisome position: 83.25

GC content: 45.99

Gene sequence:

>2244_bases
ATGGATAATATAACCCCCAAAGAAATAGCAGATGAAGAGATGATCAATCAGGCGTTCCAGGAACTTCTGAATGATTATCT
CCATACTAAACATCGCAAACGAGTTGAAATCATCACAAAAGCTTTCAACTTCGCTAATCAGGCACACAAAGGAATCAAAC
GCCGATCCGGCGAACCTTATATCATGCACCCGATTGCCGTTGCACAAATAGTATGTAACGAAATTGGTCTTGGTTCTACT
TCTATCTGTGCGGCACTGCTGCACGACGTAGTAGAAGACACTGATTACACGGTAGAAGACATCGAAAATATCTTTGGACC
GAAGATTGCCCAGATTGTAGACGGACTGACCAAAATCTCCGGAGGGATCTTCGGTGACCGTGCTTCGGCACAGGCAGAGA
ACTTCAAGAAGTTACTGCTCACCATGTCGAACGATATCCGAGTTATCCTGATCAAAATAGCCGACCGCTTGCACAATATG
CGCACGCTGGGTTCCATGCTGCCCAACAAGCAATATAAGATAGCAGGAGAAACTCTCTACATCTATGCCCCGTTAGCCAA
CCGCCTAGGTCTGTACAAGATAAAAACAGAGCTTGAAAACCTCAGCTTCAAATATGAACACCCCGAAGAATATGCCGAGA
TAGAAGAAAAGCTGAATGCTACCGCCGCCGAACGCGACAAAGTATTCAACGACTTCACAGCTCCGATCCGCACCCAGCTG
GATAAGATGGGGCTGAAATACCGGATTTTGGCACGTGTGAAATCGATCTACTCCATCTGGAACAAGATGCAGACCAAACA
CGTCCCGTTCGAGGAGATTTATGACTTGCTCGCTGTCCGCATCATCTTCGAACCACGCAACGTGGAAGAAGAGCTCAACG
ACTGTTTCGACATCTATGTCTCCATTTCCAAGATATACAAGCCTCATCCCGACCGCCTACGCGACTGGGTAAGCCACCCG
AAGGCGAATGGTTACCAAGCATTGCACGTCACCCTGATGGGAAACAACGGACAATGGATCGAGGTACAGATTCGCAGCGA
ACGGATGAACGATGTTGCAGAACAAGGCTTTGCCGCCCACTGGAAATATAAAGAAGGAGGAGGCAGCGAAGACGAAGGTG
AACTGGAGAAATGGCTGAAAACCATTAAAGAGATACTGGATGATCCGCAACCGGACGCAATCGACTTTTTGGATACGATC
AAACTGAACCTGTTTGCTTCCGAAATCTTCGTGTTCACTCCGAAGGGAGAGTTAAAGACAATGCCACAAAACTCTACGGC
ACTGGATTTTGCCTTCTCACTGCACACAGATATCGGTAGCCATTGTATCGGCGCCAAGGTCAATCATAAACTGGTGCCGC
TAAGTCATAAACTGCAAAGTGGAGATCAGGTTGAAATATTGACCTCCAAATCCCAACGTGTACAACCGCAATGGGAGGTA
TTTGCAACGACCGCCCGTGCCCGTGCCAAGATCGCAGCGATTCTTCGCAAAGAACGGAAAGCGAATCAGAAAATTGGCGA
AGAGCTACTCAACGAGTTTCTGAAGAAGGAAGAAATACGTCCGGAAGAAGCTGTCATCGAGAAGTTGCGTAAATTTCACA
ACTTCAAGAATGAAGAAGAGTTACTGGCCGCTATCGGCAGCAAAGCCATTACTTTGGGAGAAGCGGACAAGAACGAGCTG
AGAGAAAAGCAAACCAGCAACTGGAAGAAATACCTGACGTTCTCCTTCGGGAACAGCAACAAGGAAAAACCGGAAGAGAA
AGAACCGCAGGAAAAAGAAAAGATCAATCCGAAAGAGATTCTTAAACTGACCGAAGAAAGCCTGCAAAAGAAATACATCA
TGGCCGAATGTTGTCATCCGATTCCCGGTGATGATGTTTTGGGTTACGTTGATGAAAACGACCGCATTATTATTCATAAA
CGCCAGTGCCCTGTTGCTGCCAAGCTGAAAAGCAGCTATGGTAACCGTATATTAGCGACCGAATGGGATACTCACAAAGA
ACTTTCTTTCCTCGTATATATCTATTTAAGAGGTATAGACAGCATGGGACTTCTGAATGAAGTGACTCAGGTCATTTCCA
GACAGCTCAACGTGAACATCCGTAAACTTGCCATTGAAACGAATGACGGGATATTCGAAGGAAAGATTCAGCTATGGGTA
CACGATGTGGAAGATGTAAAGACAATCTGTAATAATCTGAAGAAGATTCAAAACATCAAACAGGTGAACCGGGTGGAGGA
ATAA

Upstream 100 bases:

>100_bases
AGGAAAACAATTGAAAATCTACAAATAAATGACTTCCCGCTTGCTTCATTCGGAAATTTGCTTATTTTTGCAAAAATAAG
CGAATGAAAGGATAACGAAT

Downstream 100 bases:

>100_bases
TTTATCGTCCGTCCAGTTCTTTCTTGATAGCCAGAAGCTCTTCCTTAATACCTTTCAGACGGTTCACAATTTCGTAATTG
CGAACCGTGGCTTCCTTATT

Product: GTP pyrophosphokinase

Products: NA

Alternate protein names: Penta-phosphate guanosine-3'-pyrophosphohydrolase; (ppGpp)ase [H]

Number of amino acids: Translated: 747; Mature: 747

Protein sequence:

>747_residues
MDNITPKEIADEEMINQAFQELLNDYLHTKHRKRVEIITKAFNFANQAHKGIKRRSGEPYIMHPIAVAQIVCNEIGLGST
SICAALLHDVVEDTDYTVEDIENIFGPKIAQIVDGLTKISGGIFGDRASAQAENFKKLLLTMSNDIRVILIKIADRLHNM
RTLGSMLPNKQYKIAGETLYIYAPLANRLGLYKIKTELENLSFKYEHPEEYAEIEEKLNATAAERDKVFNDFTAPIRTQL
DKMGLKYRILARVKSIYSIWNKMQTKHVPFEEIYDLLAVRIIFEPRNVEEELNDCFDIYVSISKIYKPHPDRLRDWVSHP
KANGYQALHVTLMGNNGQWIEVQIRSERMNDVAEQGFAAHWKYKEGGGSEDEGELEKWLKTIKEILDDPQPDAIDFLDTI
KLNLFASEIFVFTPKGELKTMPQNSTALDFAFSLHTDIGSHCIGAKVNHKLVPLSHKLQSGDQVEILTSKSQRVQPQWEV
FATTARARAKIAAILRKERKANQKIGEELLNEFLKKEEIRPEEAVIEKLRKFHNFKNEEELLAAIGSKAITLGEADKNEL
REKQTSNWKKYLTFSFGNSNKEKPEEKEPQEKEKINPKEILKLTEESLQKKYIMAECCHPIPGDDVLGYVDENDRIIIHK
RQCPVAAKLKSSYGNRILATEWDTHKELSFLVYIYLRGIDSMGLLNEVTQVISRQLNVNIRKLAIETNDGIFEGKIQLWV
HDVEDVKTICNNLKKIQNIKQVNRVEE

Sequences:

>Translated_747_residues
MDNITPKEIADEEMINQAFQELLNDYLHTKHRKRVEIITKAFNFANQAHKGIKRRSGEPYIMHPIAVAQIVCNEIGLGST
SICAALLHDVVEDTDYTVEDIENIFGPKIAQIVDGLTKISGGIFGDRASAQAENFKKLLLTMSNDIRVILIKIADRLHNM
RTLGSMLPNKQYKIAGETLYIYAPLANRLGLYKIKTELENLSFKYEHPEEYAEIEEKLNATAAERDKVFNDFTAPIRTQL
DKMGLKYRILARVKSIYSIWNKMQTKHVPFEEIYDLLAVRIIFEPRNVEEELNDCFDIYVSISKIYKPHPDRLRDWVSHP
KANGYQALHVTLMGNNGQWIEVQIRSERMNDVAEQGFAAHWKYKEGGGSEDEGELEKWLKTIKEILDDPQPDAIDFLDTI
KLNLFASEIFVFTPKGELKTMPQNSTALDFAFSLHTDIGSHCIGAKVNHKLVPLSHKLQSGDQVEILTSKSQRVQPQWEV
FATTARARAKIAAILRKERKANQKIGEELLNEFLKKEEIRPEEAVIEKLRKFHNFKNEEELLAAIGSKAITLGEADKNEL
REKQTSNWKKYLTFSFGNSNKEKPEEKEPQEKEKINPKEILKLTEESLQKKYIMAECCHPIPGDDVLGYVDENDRIIIHK
RQCPVAAKLKSSYGNRILATEWDTHKELSFLVYIYLRGIDSMGLLNEVTQVISRQLNVNIRKLAIETNDGIFEGKIQLWV
HDVEDVKTICNNLKKIQNIKQVNRVEE
>Mature_747_residues
MDNITPKEIADEEMINQAFQELLNDYLHTKHRKRVEIITKAFNFANQAHKGIKRRSGEPYIMHPIAVAQIVCNEIGLGST
SICAALLHDVVEDTDYTVEDIENIFGPKIAQIVDGLTKISGGIFGDRASAQAENFKKLLLTMSNDIRVILIKIADRLHNM
RTLGSMLPNKQYKIAGETLYIYAPLANRLGLYKIKTELENLSFKYEHPEEYAEIEEKLNATAAERDKVFNDFTAPIRTQL
DKMGLKYRILARVKSIYSIWNKMQTKHVPFEEIYDLLAVRIIFEPRNVEEELNDCFDIYVSISKIYKPHPDRLRDWVSHP
KANGYQALHVTLMGNNGQWIEVQIRSERMNDVAEQGFAAHWKYKEGGGSEDEGELEKWLKTIKEILDDPQPDAIDFLDTI
KLNLFASEIFVFTPKGELKTMPQNSTALDFAFSLHTDIGSHCIGAKVNHKLVPLSHKLQSGDQVEILTSKSQRVQPQWEV
FATTARARAKIAAILRKERKANQKIGEELLNEFLKKEEIRPEEAVIEKLRKFHNFKNEEELLAAIGSKAITLGEADKNEL
REKQTSNWKKYLTFSFGNSNKEKPEEKEPQEKEKINPKEILKLTEESLQKKYIMAECCHPIPGDDVLGYVDENDRIIIHK
RQCPVAAKLKSSYGNRILATEWDTHKELSFLVYIYLRGIDSMGLLNEVTQVISRQLNVNIRKLAIETNDGIFEGKIQLWV
HDVEDVKTICNNLKKIQNIKQVNRVEE

Specific function: In eubacteria ppGpp (guanosine 3'-diphosphate 5-' diphosphate) is a mediator of the stringent response that coordinates a variety of cellular activities in response to changes in nutritional abundance [H]

COG id: COG0317

COG function: function code TK; Guanosine polyphosphate pyrophosphohydrolases/synthetases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HD domain [H]

Homologues:

Organism=Homo sapiens, GI38348360, Length=134, Percent_Identity=34.3283582089552, Blast_Score=70, Evalue=8e-12,
Organism=Escherichia coli, GI1790082, Length=736, Percent_Identity=35.3260869565217, Blast_Score=451, Evalue=1e-128,
Organism=Escherichia coli, GI1789147, Length=606, Percent_Identity=33.6633663366337, Blast_Score=353, Evalue=2e-98,
Organism=Caenorhabditis elegans, GI25143118, Length=134, Percent_Identity=35.8208955223881, Blast_Score=81, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24650996, Length=147, Percent_Identity=31.9727891156463, Blast_Score=72, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002912
- InterPro:   IPR012675
- InterPro:   IPR003607
- InterPro:   IPR007685
- InterPro:   IPR004811
- InterPro:   IPR004095
- InterPro:   IPR012676 [H]

Pfam domain/function: PF01842 ACT; PF04607 RelA_SpoT; PF02824 TGS [H]

EC number: =3.1.7.2 [H]

Molecular weight: Translated: 85786; Mature: 85786

Theoretical pI: Translated: 6.72; Mature: 6.72

Prosite motif: PS00090 NITROGENASE_1_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDNITPKEIADEEMINQAFQELLNDYLHTKHRKRVEIITKAFNFANQAHKGIKRRSGEPY
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCE
IMHPIAVAQIVCNEIGLGSTSICAALLHDVVEDTDYTVEDIENIFGPKIAQIVDGLTKIS
EEHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHCCHHHHHHHHHHHHC
GGIFGDRASAQAENFKKLLLTMSNDIRVILIKIADRLHNMRTLGSMLPNKQYKIAGETLY
CCCCCCCCHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHCCCCCEEEECCEEE
IYAPLANRLGLYKIKTELENLSFKYEHPEEYAEIEEKLNATAAERDKVFNDFTAPIRTQL
EEECHHHCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHH
DKMGLKYRILARVKSIYSIWNKMQTKHVPFEEIYDLLAVRIIFEPRNVEEELNDCFDIYV
HHCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHEECCCCHHHHHHHHHHHHH
SISKIYKPHPDRLRDWVSHPKANGYQALHVTLMGNNGQWIEVQIRSERMNDVAEQGFAAH
HHHHHHCCCHHHHHHHHCCCCCCCEEEEEEEEECCCCCEEEEEECHHHHHHHHHCCCCEE
WKYKEGGGSEDEGELEKWLKTIKEILDDPQPDAIDFLDTIKLNLFASEIFVFTPKGELKT
EEECCCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHEEEEEEEEECCCCCCEE
MPQNSTALDFAFSLHTDIGSHCIGAKVNHKLVPLSHKLQSGDQVEILTSKSQRVQPQWEV
CCCCCCCEEEEEEHHHCCCCCCCCCCCCCEEECCHHHHCCCCEEEEEECCCCCCCCHHHH
FATTARARAKIAAILRKERKANQKIGEELLNEFLKKEEIRPEEAVIEKLRKFHNFKNEEE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCHHH
LLAAIGSKAITLGEADKNELREKQTSNWKKYLTFSFGNSNKEKPEEKEPQEKEKINPKEI
HHHHHCCCEEEECCCCHHHHHHHHHCCCEEEEEEEECCCCCCCCCCCCCCHHHCCCHHHH
LKLTEESLQKKYIMAECCHPIPGDDVLGYVDENDRIIIHKRQCPVAAKLKSSYGNRILAT
HHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCEEEEEECCCCHHHHHHHHCCCEEEEE
EWDTHKELSFLVYIYLRGIDSMGLLNEVTQVISRQLNVNIRKLAIETNDGIFEGKIQLWV
CCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCEEEEEEEECCCCEEEEEEEEEE
HDVEDVKTICNNLKKIQNIKQVNRVEE
ECHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MDNITPKEIADEEMINQAFQELLNDYLHTKHRKRVEIITKAFNFANQAHKGIKRRSGEPY
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCE
IMHPIAVAQIVCNEIGLGSTSICAALLHDVVEDTDYTVEDIENIFGPKIAQIVDGLTKIS
EEHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHCCHHHHHHHHHHHHC
GGIFGDRASAQAENFKKLLLTMSNDIRVILIKIADRLHNMRTLGSMLPNKQYKIAGETLY
CCCCCCCCHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHCCCCCEEEECCEEE
IYAPLANRLGLYKIKTELENLSFKYEHPEEYAEIEEKLNATAAERDKVFNDFTAPIRTQL
EEECHHHCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHH
DKMGLKYRILARVKSIYSIWNKMQTKHVPFEEIYDLLAVRIIFEPRNVEEELNDCFDIYV
HHCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHEECCCCHHHHHHHHHHHHH
SISKIYKPHPDRLRDWVSHPKANGYQALHVTLMGNNGQWIEVQIRSERMNDVAEQGFAAH
HHHHHHCCCHHHHHHHHCCCCCCCEEEEEEEEECCCCCEEEEEECHHHHHHHHHCCCCEE
WKYKEGGGSEDEGELEKWLKTIKEILDDPQPDAIDFLDTIKLNLFASEIFVFTPKGELKT
EEECCCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHEEEEEEEEECCCCCCEE
MPQNSTALDFAFSLHTDIGSHCIGAKVNHKLVPLSHKLQSGDQVEILTSKSQRVQPQWEV
CCCCCCCEEEEEEHHHCCCCCCCCCCCCCEEECCHHHHCCCCEEEEEECCCCCCCCHHHH
FATTARARAKIAAILRKERKANQKIGEELLNEFLKKEEIRPEEAVIEKLRKFHNFKNEEE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCHHH
LLAAIGSKAITLGEADKNELREKQTSNWKKYLTFSFGNSNKEKPEEKEPQEKEKINPKEI
HHHHHCCCEEEECCCCHHHHHHHHHCCCEEEEEEEECCCCCCCCCCCCCCHHHCCCHHHH
LKLTEESLQKKYIMAECCHPIPGDDVLGYVDENDRIIIHKRQCPVAAKLKSSYGNRILAT
HHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCEEEEEECCCCHHHHHHHHCCCEEEEE
EWDTHKELSFLVYIYLRGIDSMGLLNEVTQVISRQLNVNIRKLAIETNDGIFEGKIQLWV
CCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCEEEEEEEECCCCEEEEEEEEEE
HDVEDVKTICNNLKKIQNIKQVNRVEE
ECHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]