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The map label for this gene is dam [C]

Identifier: 29144581

GI number: 29144581

Start: 4485815

End: 4486675

Strand: Reverse

Name: dam [C]

Synonym: t4329

Alternate gene names: 29144581

Gene position: 4486675-4485815 (Counterclockwise)

Preceding gene: 29144582

Following gene: 29144580

Centisome position: 93.63

GC content: 46.57

Gene sequence:

>861_bases
ATGAGCACCATCCTGAAATGGGCGGGAAATAAAACCGCCATCATGCCGGAACTGATTAAGCATCTGCCTGCAGGTCAGCG
ACTGGTGGAGCCGTTCGCCGGTTCCTGTGCTGTGATGATGGCAACAGACTATCCTCATTATCTTGTCGCGGATATTAATC
CAGATCTTATCAATCTCTATAAAAAAATTGCCCTTGATTGTGAAGCTTTCATATCACGCGCAAAGAATATTTTTGCGATT
GCGAATAGAGAAGTTGCTTATTACAACATTAGGCATGAATTTAATCATTCCTCTGAAATTACTGATTTCATGAAAGCAGT
ATATTTCCTTTATCTCAATCGTCATGGTTATCGTGGACTGTGCCGCTATAACCTGAGCGGTCATTTTAATGTCCCTTACG
GTAATTATAAAAATCCGTACTTCCCTGAAAACGAAATACGTGCTTTTGCAAAAAAGGCACAACGCGCAACGTTTATCTGC
GCCAGCTATGATGAGACGCTGGCAATGCTGCGGGCGGGGGATGTGGTTTATTGTGATCCGCCATATGACGGCACATTTAG
CGGTTATCACACTGCCAGTTTTACCGGGGACGATCAGTACCATCTGGCGTCTATTCTTGAACGCCGGTCATCAGAAGGTC
ATCCGATTATTGTGTCCAACAGCGACACCTCTCTGACACGTTCCTTATATCGTAATTTCACCCGCCACCGCATCATCGCA
AAGCGCAGCATGGGTGTGGCTGCAGGTGATGGTAAATCTGCAACAGAAATCATCGCTACGAAGTTAGTGCGCTGGTTCGG
TGTTGATTTGGCGTCTGGTCCTGATGTCTCGGTGGAAACTGAGGTGCGGGCGTGGCAGTGA

Upstream 100 bases:

>100_bases
CGCATGAAGGTGTTGTAGTTGAAGGTAAGGCGCACCGTGCTTTAGCTGATTGCAAAATGACGTTAGGGCTTGTCCGTGCT
CTGGCTAAAGGTGGTGCTAA

Downstream 100 bases:

>100_bases
GTAAAATCACATTACATTATGCACAAACCACCGGCGGCTCGAATGAGGCCGCCGCAGCCTTTCCATGGAATACCCCAAAA
AAAGCGGTTAACCCGTATCT

Product: DNA adenine methylase

Products: NA

Alternate protein names: M.Eco67Dam; ORF1-EC67 DAM [H]

Number of amino acids: Translated: 286; Mature: 285

Protein sequence:

>286_residues
MSTILKWAGNKTAIMPELIKHLPAGQRLVEPFAGSCAVMMATDYPHYLVADINPDLINLYKKIALDCEAFISRAKNIFAI
ANREVAYYNIRHEFNHSSEITDFMKAVYFLYLNRHGYRGLCRYNLSGHFNVPYGNYKNPYFPENEIRAFAKKAQRATFIC
ASYDETLAMLRAGDVVYCDPPYDGTFSGYHTASFTGDDQYHLASILERRSSEGHPIIVSNSDTSLTRSLYRNFTRHRIIA
KRSMGVAAGDGKSATEIIATKLVRWFGVDLASGPDVSVETEVRAWQ

Sequences:

>Translated_286_residues
MSTILKWAGNKTAIMPELIKHLPAGQRLVEPFAGSCAVMMATDYPHYLVADINPDLINLYKKIALDCEAFISRAKNIFAI
ANREVAYYNIRHEFNHSSEITDFMKAVYFLYLNRHGYRGLCRYNLSGHFNVPYGNYKNPYFPENEIRAFAKKAQRATFIC
ASYDETLAMLRAGDVVYCDPPYDGTFSGYHTASFTGDDQYHLASILERRSSEGHPIIVSNSDTSLTRSLYRNFTRHRIIA
KRSMGVAAGDGKSATEIIATKLVRWFGVDLASGPDVSVETEVRAWQ
>Mature_285_residues
STILKWAGNKTAIMPELIKHLPAGQRLVEPFAGSCAVMMATDYPHYLVADINPDLINLYKKIALDCEAFISRAKNIFAIA
NREVAYYNIRHEFNHSSEITDFMKAVYFLYLNRHGYRGLCRYNLSGHFNVPYGNYKNPYFPENEIRAFAKKAQRATFICA
SYDETLAMLRAGDVVYCDPPYDGTFSGYHTASFTGDDQYHLASILERRSSEGHPIIVSNSDTSLTRSLYRNFTRHRIIAK
RSMGVAAGDGKSATEIIATKLVRWFGVDLASGPDVSVETEVRAWQ

Specific function: Methylates DNA within the sequence GATC and protects the DNA from cleavage by the restriction endonuclease MboI. May play a regulatory role in the functions of the retron [H]

COG id: COG0338

COG function: function code L; Site-specific DNA methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N(4)/N(6)-methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1789789, Length=262, Percent_Identity=45.4198473282443, Blast_Score=236, Evalue=1e-63,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023095
- InterPro:   IPR002052
- InterPro:   IPR012327 [H]

Pfam domain/function: PF02086 MethyltransfD12 [H]

EC number: =2.1.1.72 [H]

Molecular weight: Translated: 32311; Mature: 32180

Theoretical pI: Translated: 8.43; Mature: 8.43

Prosite motif: PS00092 N6_MTASE ; PS00142 ZINC_PROTEASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTILKWAGNKTAIMPELIKHLPAGQRLVEPFAGSCAVMMATDYPHYLVADINPDLINLY
CCCCEECCCCCCCHHHHHHHHCCCCHHHHHHCCCCEEEEEECCCCCEEEEECCHHHHHHH
KKIALDCEAFISRAKNIFAIANREVAYYNIRHEFNHSSEITDFMKAVYFLYLNRHGYRGL
HHHHHHHHHHHHHCCCEEEEECCEEEEEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCCE
CRYNLSGHFNVPYGNYKNPYFPENEIRAFAKKAQRATFICASYDETLAMLRAGDVVYCDP
EEECCCCEEECCCCCCCCCCCCHHHHHHHHHHHHHHEEEEECHHHHHHHHHCCCEEEECC
PYDGTFSGYHTASFTGDDQYHLASILERRSSEGHPIIVSNSDTSLTRSLYRNFTRHRIIA
CCCCCCCCEEEEEECCCCHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHHHHHH
KRSMGVAAGDGKSATEIIATKLVRWFGVDLASGPDVSVETEVRAWQ
HHCCCEECCCCCHHHHHHHHHHHHHHCCCCCCCCCCEEECCCEECC
>Mature Secondary Structure 
STILKWAGNKTAIMPELIKHLPAGQRLVEPFAGSCAVMMATDYPHYLVADINPDLINLY
CCCEECCCCCCCHHHHHHHHCCCCHHHHHHCCCCEEEEEECCCCCEEEEECCHHHHHHH
KKIALDCEAFISRAKNIFAIANREVAYYNIRHEFNHSSEITDFMKAVYFLYLNRHGYRGL
HHHHHHHHHHHHHCCCEEEEECCEEEEEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCCE
CRYNLSGHFNVPYGNYKNPYFPENEIRAFAKKAQRATFICASYDETLAMLRAGDVVYCDP
EEECCCCEEECCCCCCCCCCCCHHHHHHHHHHHHHHEEEEECHHHHHHHHHCCCEEEECC
PYDGTFSGYHTASFTGDDQYHLASILERRSSEGHPIIVSNSDTSLTRSLYRNFTRHRIIA
CCCCCCCCEEEEEECCCCHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHHHHHH
KRSMGVAAGDGKSATEIIATKLVRWFGVDLASGPDVSVETEVRAWQ
HHCCCEECCCCCHHHHHHHHHHHHHHCCCCCCCCCCEEECCCEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1701261 [H]