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The map label for this gene is metR [H]

Identifier: 29143659

GI number: 29143659

Start: 3435638

End: 3436591

Strand: Direct

Name: metR [H]

Synonym: t3333

Alternate gene names: 29143659

Gene position: 3435638-3436591 (Clockwise)

Preceding gene: 29143656

Following gene: 29143663

Centisome position: 71.7

GC content: 58.49

Gene sequence:

>954_bases
ATGATCGAAATTAAACATCTGAAAACACTCCAGGCATTGCGGAATAGTGGATCGCTGGCTGCTGCTGCTGCGGTGCTGCA
CCAGACTCAATCCGCTCTGTCTCATCAGTTCAGCGATCTGGAACAACGCCTTGGCTTCCGCCTGTTCGTGCGTAAAAGCC
AGCCGCTGCGCTTTACGCCGCAGGGGGAAGTTCTGCTGCAACTGGCAAACCAGGTGCTGCCGCAAATCAGCCGTGCGCTG
CAGGCGTGCAACGAACCGCAGCAAACTCGTCTGCGCATCGCTATTGAATGTCATAGCTGTATTCAGTGGCTGACCCCGGC
GCTGGAAAACTTCCGTGCAAGCTGGCCGCAGGTAGAAATGGATTTCACATCCGGCGTCACTTTTGATCCGCAACCGGCCT
TGCAACAAGGCGAGCTGGATTTGGTGATGACGTCGGATATTTTGCCGCGCAGCGGGCTGCATTATTCGCCGATGTTTGAT
TTTGAAGTCCGACTGGTGCTGGCGCCCGACCATCCGCTGGCCAGCAAAACGCAGATTACGCCGGAAGATTTAGCCAGCGA
AACGCTGTTGATTTACCCGGTGCAGCGCAGCCGGCTGGATGTCTGGCGACATTTCCTGCAACCGGCGGGTATCAGTCCGC
TGCTGAAAAGCGTGGATAATACGCTACTGCTGATTCAGATGGTGGCGGCCAGAATGGGTATTGCCGCCCTGCCGCACTGG
GTGGTGGAAAGCGTTGAGCGCCAGGGTCTGGTGGTGACCAAAACCCTGGGCGATGGTCTGTGGAGCCGCCTGTATGCCGC
CGTGCGCGACGGCGACCAGCGTCAGGCGGTGACCGAGGCGTTTATTCGCTCGACGCGGGATCACGCCTGCGATCATCTGC
CGTTTGTGCGGAGCGCGGAGCGACCCATTTTCGATGCACCCACAGCGAAGCCAGGATCACAGCCGCGCCTGTAA

Upstream 100 bases:

>100_bases
TATGACGAGTTTTAGACGTCTGGATGTTTACACATCCATAATGTAAAGGTACTGTATATTCCTCAAGCGCAATTTGTTCA
TGGCGAAGTGAAGGACTTTC

Downstream 100 bases:

>100_bases
TAAAGCTTGGCCAGTGGGGCTGCTGATGCCAGATGGCAAGATTAACCAGCAGTCCTGCGGGAACATGCATATTATTCATG
ATACCCAGCGTCCCGGCGTC

Product: trans-activator of metE and metH

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 317; Mature: 317

Protein sequence:

>317_residues
MIEIKHLKTLQALRNSGSLAAAAAVLHQTQSALSHQFSDLEQRLGFRLFVRKSQPLRFTPQGEVLLQLANQVLPQISRAL
QACNEPQQTRLRIAIECHSCIQWLTPALENFRASWPQVEMDFTSGVTFDPQPALQQGELDLVMTSDILPRSGLHYSPMFD
FEVRLVLAPDHPLASKTQITPEDLASETLLIYPVQRSRLDVWRHFLQPAGISPLLKSVDNTLLLIQMVAARMGIAALPHW
VVESVERQGLVVTKTLGDGLWSRLYAAVRDGDQRQAVTEAFIRSTRDHACDHLPFVRSAERPIFDAPTAKPGSQPRL

Sequences:

>Translated_317_residues
MIEIKHLKTLQALRNSGSLAAAAAVLHQTQSALSHQFSDLEQRLGFRLFVRKSQPLRFTPQGEVLLQLANQVLPQISRAL
QACNEPQQTRLRIAIECHSCIQWLTPALENFRASWPQVEMDFTSGVTFDPQPALQQGELDLVMTSDILPRSGLHYSPMFD
FEVRLVLAPDHPLASKTQITPEDLASETLLIYPVQRSRLDVWRHFLQPAGISPLLKSVDNTLLLIQMVAARMGIAALPHW
VVESVERQGLVVTKTLGDGLWSRLYAAVRDGDQRQAVTEAFIRSTRDHACDHLPFVRSAERPIFDAPTAKPGSQPRL
>Mature_317_residues
MIEIKHLKTLQALRNSGSLAAAAAVLHQTQSALSHQFSDLEQRLGFRLFVRKSQPLRFTPQGEVLLQLANQVLPQISRAL
QACNEPQQTRLRIAIECHSCIQWLTPALENFRASWPQVEMDFTSGVTFDPQPALQQGELDLVMTSDILPRSGLHYSPMFD
FEVRLVLAPDHPLASKTQITPEDLASETLLIYPVQRSRLDVWRHFLQPAGISPLLKSVDNTLLLIQMVAARMGIAALPHW
VVESVERQGLVVTKTLGDGLWSRLYAAVRDGDQRQAVTEAFIRSTRDHACDHLPFVRSAERPIFDAPTAKPGSQPRL

Specific function: Control of the last step in methionine biosynthesis; metR is a positive activator of the metA, metE and metH genes. It is also a negative regulator of its own expression [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790262, Length=317, Percent_Identity=91.4826498422713, Blast_Score=602, Evalue=1e-173,
Organism=Escherichia coli, GI157672245, Length=212, Percent_Identity=27.8301886792453, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1788887, Length=245, Percent_Identity=28.1632653061224, Blast_Score=82, Evalue=6e-17,
Organism=Escherichia coli, GI1787530, Length=252, Percent_Identity=25, Blast_Score=75, Evalue=6e-15,
Organism=Escherichia coli, GI87082024, Length=173, Percent_Identity=31.2138728323699, Blast_Score=74, Evalue=9e-15,
Organism=Escherichia coli, GI1787806, Length=251, Percent_Identity=23.1075697211155, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1787879, Length=245, Percent_Identity=25.7142857142857, Blast_Score=72, Evalue=6e-14,
Organism=Escherichia coli, GI1790208, Length=254, Percent_Identity=28.740157480315, Blast_Score=72, Evalue=6e-14,
Organism=Escherichia coli, GI1789204, Length=211, Percent_Identity=27.9620853080569, Blast_Score=71, Evalue=9e-14,
Organism=Escherichia coli, GI1788296, Length=243, Percent_Identity=26.3374485596708, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1786401, Length=254, Percent_Identity=24.8031496062992, Blast_Score=65, Evalue=5e-12,
Organism=Escherichia coli, GI1790399, Length=255, Percent_Identity=27.843137254902, Blast_Score=65, Evalue=5e-12,
Organism=Escherichia coli, GI87081904, Length=219, Percent_Identity=27.8538812785388, Blast_Score=65, Evalue=6e-12,
Organism=Escherichia coli, GI1789173, Length=209, Percent_Identity=26.3157894736842, Blast_Score=62, Evalue=6e-11,
Organism=Escherichia coli, GI1789506, Length=188, Percent_Identity=25, Blast_Score=62, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 35513; Mature: 35513

Theoretical pI: Translated: 7.67; Mature: 7.67

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIEIKHLKTLQALRNSGSLAAAAAVLHQTQSALSHQFSDLEQRLGFRLFVRKSQPLRFTP
CCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCEEECC
QGEVLLQLANQVLPQISRALQACNEPQQTRLRIAIECHSCIQWLTPALENFRASWPQVEM
CHHHHHHHHHHHHHHHHHHHHHCCCCCHHHEEEEEEHHHHHHHHHHHHHHHHCCCCCEEE
DFTSGVTFDPQPALQQGELDLVMTSDILPRSGLHYSPMFDFEVRLVLAPDHPLASKTQIT
ECCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCEEEEEEEECCCCCCCCCCCC
PEDLASETLLIYPVQRSRLDVWRHFLQPAGISPLLKSVDNTLLLIQMVAARMGIAALPHW
HHHHCCCEEEEEECHHHHHHHHHHHHCCCCCCHHHHHHCHHHHHHHHHHHHHHHHHHHHH
VVESVERQGLVVTKTLGDGLWSRLYAAVRDGDQRQAVTEAFIRSTRDHACDHLPFVRSAE
HHHHHHHCCEEEEEHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCHHHCCC
RPIFDAPTAKPGSQPRL
CCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MIEIKHLKTLQALRNSGSLAAAAAVLHQTQSALSHQFSDLEQRLGFRLFVRKSQPLRFTP
CCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCEEECC
QGEVLLQLANQVLPQISRALQACNEPQQTRLRIAIECHSCIQWLTPALENFRASWPQVEM
CHHHHHHHHHHHHHHHHHHHHHCCCCCHHHEEEEEEHHHHHHHHHHHHHHHHCCCCCEEE
DFTSGVTFDPQPALQQGELDLVMTSDILPRSGLHYSPMFDFEVRLVLAPDHPLASKTQIT
ECCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCEEEEEEEECCCCCCCCCCCC
PEDLASETLLIYPVQRSRLDVWRHFLQPAGISPLLKSVDNTLLLIQMVAARMGIAALPHW
HHHHCCCEEEEEECHHHHHHHHHHHHCCCCCCHHHHHHCHHHHHHHHHHHHHHHHHHHHH
VVESVERQGLVVTKTLGDGLWSRLYAAVRDGDQRQAVTEAFIRSTRDHACDHLPFVRSAE
HHHHHHHCCEEEEEHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCHHHCCC
RPIFDAPTAKPGSQPRL
CCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]