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The map label for this gene is rhtB

Identifier: 29143663

GI number: 29143663

Start: 3439402

End: 3440022

Strand: Direct

Name: rhtB

Synonym: t3337

Alternate gene names: 29143663

Gene position: 3439402-3440022 (Clockwise)

Preceding gene: 29143659

Following gene: 29143667

Centisome position: 71.77

GC content: 54.91

Gene sequence:

>621_bases
ATGACCTTTGAATGGTGGTTCGCCTATCTACTGACCTCAACTCTGTTGAGTCTTTCTCCGGGTTCAGGCGCCATCAATAC
CATGACGACGTCTATCAACCATGGATATCGTGGCGCAGCGGCTTCTATCGCCGGGCTCCAGACCGGGCTGGGGATACATA
TCGTACTGGTGGGCGTCGGACTGGGTACGCTCTTTTCGCGCTCGCTCATCGCTTTTGAAATTCTGAAATGGGCTGGCGCG
GCTTATCTTATCTGGCTGGGTATCCAGCAATGGCGCGCCGCAGGCGCTATCGATCTGCATACTCTCGCCCAGACGCAATC
GCGGGGTCGGCTGTTCAAACGGGCGATATTTGTCAATCTAACCAATCCCAAAAGTATTGTCTTTCTTGCCGCCCTGTTTC
CGCAATTCATCATGCCGCAGCAACCGCAACTGGCGCAGTACCTCATTCTCGGCGTCACCACGATTGTGGTGGATATGATT
GTGATGACCGGTTACGCCACGCTGGCGCAGCGCATTGCCGCCTGGATTAAAGGACCAAAGCAGATGAAGGCGCTGAATAA
AGCGTTTGGTTCGTTGTTTATGCTGGTAGGCGCGCTCCTGGCGTCGGCAAGACACGCGTGA

Upstream 100 bases:

>100_bases
GCCTCGTTTACCAGGTAAAATCGTTTTTTGCGTCGTGCCTGACACAACACAGCGGCAGTAGCGTATTGTGGCACAAAAAC
AGACAACCAAGGAGTTTCGC

Downstream 100 bases:

>100_bases
TAGTCGTGCCGGATGATGTTTATCCGGCCCGGAGGTAAATTAACGCGAAATAATCAGGTGAATACCAAAGCCCGCAAACA
GAGCGCCGGCAAAGCCATCA

Product: homoserine/homoserine lactone efflux protein

Products: homoserine lactone [Periplasm]; Proton [Cytoplasm]; homoserine [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 206; Mature: 205

Protein sequence:

>206_residues
MTFEWWFAYLLTSTLLSLSPGSGAINTMTTSINHGYRGAAASIAGLQTGLGIHIVLVGVGLGTLFSRSLIAFEILKWAGA
AYLIWLGIQQWRAAGAIDLHTLAQTQSRGRLFKRAIFVNLTNPKSIVFLAALFPQFIMPQQPQLAQYLILGVTTIVVDMI
VMTGYATLAQRIAAWIKGPKQMKALNKAFGSLFMLVGALLASARHA

Sequences:

>Translated_206_residues
MTFEWWFAYLLTSTLLSLSPGSGAINTMTTSINHGYRGAAASIAGLQTGLGIHIVLVGVGLGTLFSRSLIAFEILKWAGA
AYLIWLGIQQWRAAGAIDLHTLAQTQSRGRLFKRAIFVNLTNPKSIVFLAALFPQFIMPQQPQLAQYLILGVTTIVVDMI
VMTGYATLAQRIAAWIKGPKQMKALNKAFGSLFMLVGALLASARHA
>Mature_205_residues
TFEWWFAYLLTSTLLSLSPGSGAINTMTTSINHGYRGAAASIAGLQTGLGIHIVLVGVGLGTLFSRSLIAFEILKWAGAA
YLIWLGIQQWRAAGAIDLHTLAQTQSRGRLFKRAIFVNLTNPKSIVFLAALFPQFIMPQQPQLAQYLILGVTTIVVDMIV
MTGYATLAQRIAAWIKGPKQMKALNKAFGSLFMLVGALLASARHA

Specific function: Conducts the efflux of homoserine and homoserine lactone

COG id: COG1280

COG function: function code E; Putative threonine efflux protein

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the rht family

Homologues:

Organism=Escherichia coli, GI48994979, Length=206, Percent_Identity=89.3203883495146, Blast_Score=372, Evalue=1e-104,
Organism=Escherichia coli, GI1788099, Length=202, Percent_Identity=27.2277227722772, Blast_Score=75, Evalue=3e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RHTB_SALTI (Q8Z3B4)

Other databases:

- EMBL:   AL627278
- EMBL:   AE014613
- RefSeq:   NP_457791.1
- RefSeq:   NP_807005.1
- GeneID:   1071508
- GeneID:   1249854
- GenomeReviews:   AE014613_GR
- GenomeReviews:   AL513382_GR
- KEGG:   stt:t3337
- KEGG:   sty:STY3599
- HOGENOM:   HBG759042
- OMA:   QWRARVE
- ProtClustDB:   PRK10520
- BioCyc:   SENT209261:T3337-MONOMER
- BioCyc:   SENT220341:STY3599-MONOMER
- InterPro:   IPR004778
- InterPro:   IPR001123
- TIGRFAMs:   TIGR00949

Pfam domain/function: PF01810 LysE

EC number: NA

Molecular weight: Translated: 22309; Mature: 22178

Theoretical pI: Translated: 11.20; Mature: 11.20

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x11bdd168)-; HASH(0x11c5ac90)-; HASH(0x119067b8)-; HASH(0x11802a24)-; HASH(0x119d8d8c)-; HASH(0x118a138c)-;

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTFEWWFAYLLTSTLLSLSPGSGAINTMTTSINHGYRGAAASIAGLQTGLGIHIVLVGVG
CCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCCHHHHHHHHHHHCCCHHHHHHHHH
LGTLFSRSLIAFEILKWAGAAYLIWLGIQQWRAAGAIDLHTLAQTQSRGRLFKRAIFVNL
HHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHEEEE
TNPKSIVFLAALFPQFIMPQQPQLAQYLILGVTTIVVDMIVMTGYATLAQRIAAWIKGPK
CCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHH
QMKALNKAFGSLFMLVGALLASARHA
HHHHHHHHHHHHHHHHHHHHHHHCCH
>Mature Secondary Structure 
TFEWWFAYLLTSTLLSLSPGSGAINTMTTSINHGYRGAAASIAGLQTGLGIHIVLVGVG
CHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCCHHHHHHHHHHHCCCHHHHHHHHH
LGTLFSRSLIAFEILKWAGAAYLIWLGIQQWRAAGAIDLHTLAQTQSRGRLFKRAIFVNL
HHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHEEEE
TNPKSIVFLAALFPQFIMPQQPQLAQYLILGVTTIVVDMIVMTGYATLAQRIAAWIKGPK
CCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHH
QMKALNKAFGSLFMLVGALLASARHA
HHHHHHHHHHHHHHHHHHHHHHHCCH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; homoserine [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + homoserine lactone [Cytoplasm] = Proton [Cytoplasm] + homoserine lactone [Periplasm] Proton [Periplasm] + homoserine [Cytoplasm] = Proton [Cytoplasm] + homoserine [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11677608; 12644504