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The map label for this gene is ascG [H]

Identifier: 29143274

GI number: 29143274

Start: 3014514

End: 3015536

Strand: Direct

Name: ascG [H]

Synonym: t2922

Alternate gene names: 29143274

Gene position: 3014514-3015536 (Clockwise)

Preceding gene: 29143273

Following gene: 29143276

Centisome position: 62.91

GC content: 56.89

Gene sequence:

>1023_bases
ATGATGGCGACAATGCTGGATGTTTCCCGCCATGCGGGCGTATCAAAGGCCACCGTCTCACGAGTGCTGAATGGGACGGG
GCAGGTAAAAGAAAGCACGCGCCAGAAAGTGTTTACGGCGATGCAGGCTCTGGACTATCGCCCCAACCTGCTGGCACGCT
CGCTGGCAAATCGCACCAGCAACAGCATCGGTCTGGTCGTCTCTACGTTTGACGGCTTCTATTTTGGCAGTTTGTTGCGC
CGGGCGTCGCGCCAGGCGGAGTTTCATAACAAGCAGTTGATCGTCACCGATGGTCACGATACGCCGGAACGAGAGCAGAA
AGCCGTACAAATGTTGGCCGACAGACAGTGCGACGCTATTATTCTTTACACTCGCTATATGGATGAGCCGACGATTTTGT
CGTTGATTGACGCCACGGAAATGCCGCTGGTGATTATTAATCGCAACATCACTCAGGCCCACGATCGCGCTATTTTCTTC
GAGCAGGAGACGGCGGCATTCCAGGCGGTGGAATACCTGATTACGCAGGGCCATCGCGATATCGCCTGTATTACGCTGCC
TGTTCATACTCCCACCGGCACATCACGCGTAGCGGGTTATCGAAAGGCGCTGGAAAAATATGGCATTCCCTGGCAACCGG
CAAAAGTGAAATACGGCGATTACACGCTGACGCGCGGCTATGACGCCTGCCGGGAATTACTGGAGGAAGGCGTCACGTTT
AGCGCGCTATTCGCCTGTAATGATGACACGGCGCTGGGCGCGGCAAAAGCGCTGCGCCAGGCCGGATTACGCATCCCGCA
GGATGTGTCGCTGTTTGGTTTTGACGATGCGCCGGGCGCAACCTGGCTTGAGCCGGGGCTTTCAACAGTCTATTTACCCA
TCGAGGATATGATAGCCACCGCAATTGATCAGGCCGTTCGTCTGGCGAACAGCGAGCCGGTCGCCCCGATCCCGCCCTTT
ACCGGCACGCTGATTCTGCGCGAGTCCGTCGCCGCGGGCCCGTTTTTTCAACGTCCGGTCTAA

Upstream 100 bases:

>100_bases
GAAGTAACTCATGTATTTAGTCCGACACTGGTACGCCGACATTCGGTGTCCACGCCGACGGATACCGGGCACCTGTCGAC
AACCGATTAATCAGGGTAGC

Downstream 100 bases:

>100_bases
TGATGCGCTTCAGAGCATCTCCAGCGCTAACAGCTCTTCAATGGTTTGACGACGGCGGATCAGCCGCGCCTGTCCATTGT
CAAACAGTACTTCCGGCAGG

Product: regulatory protein

Products: NA

Alternate protein names: Cryptic asc operon repressor [H]

Number of amino acids: Translated: 340; Mature: 340

Protein sequence:

>340_residues
MMATMLDVSRHAGVSKATVSRVLNGTGQVKESTRQKVFTAMQALDYRPNLLARSLANRTSNSIGLVVSTFDGFYFGSLLR
RASRQAEFHNKQLIVTDGHDTPEREQKAVQMLADRQCDAIILYTRYMDEPTILSLIDATEMPLVIINRNITQAHDRAIFF
EQETAAFQAVEYLITQGHRDIACITLPVHTPTGTSRVAGYRKALEKYGIPWQPAKVKYGDYTLTRGYDACRELLEEGVTF
SALFACNDDTALGAAKALRQAGLRIPQDVSLFGFDDAPGATWLEPGLSTVYLPIEDMIATAIDQAVRLANSEPVAPIPPF
TGTLILRESVAAGPFFQRPV

Sequences:

>Translated_340_residues
MMATMLDVSRHAGVSKATVSRVLNGTGQVKESTRQKVFTAMQALDYRPNLLARSLANRTSNSIGLVVSTFDGFYFGSLLR
RASRQAEFHNKQLIVTDGHDTPEREQKAVQMLADRQCDAIILYTRYMDEPTILSLIDATEMPLVIINRNITQAHDRAIFF
EQETAAFQAVEYLITQGHRDIACITLPVHTPTGTSRVAGYRKALEKYGIPWQPAKVKYGDYTLTRGYDACRELLEEGVTF
SALFACNDDTALGAAKALRQAGLRIPQDVSLFGFDDAPGATWLEPGLSTVYLPIEDMIATAIDQAVRLANSEPVAPIPPF
TGTLILRESVAAGPFFQRPV
>Mature_340_residues
MMATMLDVSRHAGVSKATVSRVLNGTGQVKESTRQKVFTAMQALDYRPNLLARSLANRTSNSIGLVVSTFDGFYFGSLLR
RASRQAEFHNKQLIVTDGHDTPEREQKAVQMLADRQCDAIILYTRYMDEPTILSLIDATEMPLVIINRNITQAHDRAIFF
EQETAAFQAVEYLITQGHRDIACITLPVHTPTGTSRVAGYRKALEKYGIPWQPAKVKYGDYTLTRGYDACRELLEEGVTF
SALFACNDDTALGAAKALRQAGLRIPQDVSLFGFDDAPGATWLEPGLSTVYLPIEDMIATAIDQAVRLANSEPVAPIPPF
TGTLILRESVAAGPFFQRPV

Specific function: Repressor of the asc operon. The cryptic operon is activated by the insertion of IS186 into the ascG gene [H]

COG id: COG1609

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789068, Length=334, Percent_Identity=40.7185628742515, Blast_Score=271, Evalue=3e-74,
Organism=Escherichia coli, GI1789202, Length=333, Percent_Identity=34.5345345345345, Blast_Score=181, Evalue=4e-47,
Organism=Escherichia coli, GI1788474, Length=333, Percent_Identity=34.2342342342342, Blast_Score=181, Evalue=8e-47,
Organism=Escherichia coli, GI1787948, Length=336, Percent_Identity=31.8452380952381, Blast_Score=176, Evalue=2e-45,
Organism=Escherichia coli, GI1790194, Length=302, Percent_Identity=31.1258278145695, Blast_Score=158, Evalue=6e-40,
Organism=Escherichia coli, GI1790369, Length=292, Percent_Identity=30.4794520547945, Blast_Score=144, Evalue=6e-36,
Organism=Escherichia coli, GI1786540, Length=330, Percent_Identity=28.4848484848485, Blast_Score=137, Evalue=7e-34,
Organism=Escherichia coli, GI1787580, Length=320, Percent_Identity=27.1875, Blast_Score=129, Evalue=3e-31,
Organism=Escherichia coli, GI48994940, Length=311, Percent_Identity=28.6173633440514, Blast_Score=121, Evalue=8e-29,
Organism=Escherichia coli, GI1790715, Length=293, Percent_Identity=24.2320819112628, Blast_Score=88, Evalue=7e-19,
Organism=Escherichia coli, GI1787906, Length=283, Percent_Identity=26.5017667844523, Blast_Score=88, Evalue=7e-19,
Organism=Escherichia coli, GI1786268, Length=151, Percent_Identity=29.8013245033113, Blast_Score=75, Evalue=7e-15,
Organism=Escherichia coli, GI1790192, Length=304, Percent_Identity=25.3289473684211, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1790689, Length=293, Percent_Identity=24.2320819112628, Blast_Score=68, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 37505; Mature: 37505

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMATMLDVSRHAGVSKATVSRVLNGTGQVKESTRQKVFTAMQALDYRPNLLARSLANRTS
CCCHHHHHHHHCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCC
NSIGLVVSTFDGFYFGSLLRRASRQAEFHNKQLIVTDGHDTPEREQKAVQMLADRQCDAI
CCEEEEEECCCHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHCCCCCEE
ILYTRYMDEPTILSLIDATEMPLVIINRNITQAHDRAIFFEQETAAFQAVEYLITQGHRD
EEEEECCCCCHHEEEECCCCCCEEEEECCCCHHHCCEEEEEHHHHHHHHHHHHHHCCCCC
IACITLPVHTPTGTSRVAGYRKALEKYGIPWQPAKVKYGDYTLTRGYDACRELLEEGVTF
EEEEEEEEECCCCCHHHHHHHHHHHHCCCCCCCCEEEECCEEEECCHHHHHHHHHCCCCE
SALFACNDDTALGAAKALRQAGLRIPQDVSLFGFDDAPGATWLEPGLSTVYLPIEDMIAT
EEEEEECCCCHHHHHHHHHHCCCCCCCCCEEEECCCCCCCCEECCCCCEEEECHHHHHHH
AIDQAVRLANSEPVAPIPPFTGTLILRESVAAGPFFQRPV
HHHHHHHHCCCCCCCCCCCCCCCEEEEHHHHCCCCCCCCC
>Mature Secondary Structure
MMATMLDVSRHAGVSKATVSRVLNGTGQVKESTRQKVFTAMQALDYRPNLLARSLANRTS
CCCHHHHHHHHCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCC
NSIGLVVSTFDGFYFGSLLRRASRQAEFHNKQLIVTDGHDTPEREQKAVQMLADRQCDAI
CCEEEEEECCCHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHCCCCCEE
ILYTRYMDEPTILSLIDATEMPLVIINRNITQAHDRAIFFEQETAAFQAVEYLITQGHRD
EEEEECCCCCHHEEEECCCCCCEEEEECCCCHHHCCEEEEEHHHHHHHHHHHHHHCCCCC
IACITLPVHTPTGTSRVAGYRKALEKYGIPWQPAKVKYGDYTLTRGYDACRELLEEGVTF
EEEEEEEEECCCCCHHHHHHHHHHHHCCCCCCCCEEEECCEEEECCHHHHHHHHHCCCCE
SALFACNDDTALGAAKALRQAGLRIPQDVSLFGFDDAPGATWLEPGLSTVYLPIEDMIAT
EEEEEECCCCHHHHHHHHHHCCCCCCCCCEEEECCCCCCCCEECCCCCEEEECHHHHHHH
AIDQAVRLANSEPVAPIPPFTGTLILRESVAAGPFFQRPV
HHHHHHHHCCCCCCCCCCCCCCCEEEEHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1630307; 9278503 [H]