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The map label for this gene is galR [H]

Identifier: 29143273

GI number: 29143273

Start: 3013475

End: 3014503

Strand: Direct

Name: galR [H]

Synonym: t2921

Alternate gene names: 29143273

Gene position: 3013475-3014503 (Clockwise)

Preceding gene: 29143269

Following gene: 29143274

Centisome position: 62.89

GC content: 56.07

Gene sequence:

>1029_bases
ATGGCGACCATAAAAGATGTAGCCCGACTGGCCGGTGTTTCAGTCGCCACCGTTTCTCGCGTTATTAACGATTCGCCAAA
AGCCAGCGAAGCGTCCCGGCTGGCGGTGACCAGCGCAATGGAGTCCCTGAGCTATCACCCTAACGCCAACGCGCGCGCGC
TGGCACAGCAGGCAACGGAAACCCTCGGTCTGGTGGTCGGCGACGTTTCCGATCCTTTTTTCGGCGCGATGGTGAAAGCC
GTTGAACAGGTGGCGTATCACACCGGCAATTTTTTACTGATTGGCAACGGGTATCATAACGAACAAAAAGAGCGTCAGGC
TATTGAACAGTTGATTCGTCATCGTTGCGCAGCGTTAGTGGTACACGCCAAAATGATTCCGGATGCGGACCTGGCCTCAT
TAATGAAGCAAATCCCCGGCATGGTGCTGATTAACCGCATTTTACCGGGGTTAGAACATCGCTGTGTCGCGCTGGATGAC
CGTTACGGGGCATGGCTGGCGACCCGGCATCTGATCCAGCAAGGTCATACGCGTATTGGGTATATCTGTTCCAATCACAC
TATCTCTGATGCCGAAGATCGCCTGAGGGGCTATTACGATGCGCTGGCGGAAAGCCATATCCCGGCTAACGATCGGCTGG
TGACGTTCGGCGAACCGGATGAAAGCGGCGGCGAGCAGGCGATGACTGAGTTATTAGGTCGCGGCAGACATTTTACCGCG
GTGGCCTGCTATAACGACTCGATGGCGGCCGGCGCGATGGGAGTATTAAATGATAATGGCGTGGGGGTGCCGGGCGAAGT
ATCGCTCATCGGTTTTGATGATGTACTGGTCTCACGCTATGTGCGTCCCCGACTGACCACCATTCGGTATCCGATCGTCA
CCATGGCGACACAGGCGGCGGAGCTGGCCTTAGCGCTGGCAGGGAAATGCCCTACGCCAGAAGTAACTCATGTATTTAGT
CCGACACTGGTACGCCGACATTCGGTGTCCACGCCGACGGATACCGGGCACCTGTCGACAACCGATTAA

Upstream 100 bases:

>100_bases
GGTCACATTTGTCCGACACGGCGGGGCAAGAGGCTTGAACAGACGTTCATTTTCCGTAAAACTGGCGTAATGTAAGCGTT
TACCCACTATAGGTATTATC

Downstream 100 bases:

>100_bases
TCAGGGTAGCATGATGGCGACAATGCTGGATGTTTCCCGCCATGCGGGCGTATCAAAGGCCACCGTCTCACGAGTGCTGA
ATGGGACGGGGCAGGTAAAA

Product: DNA-binding transcriptional regulator GalR

Products: NA

Alternate protein names: Galactose operon repressor [H]

Number of amino acids: Translated: 342; Mature: 341

Protein sequence:

>342_residues
MATIKDVARLAGVSVATVSRVINDSPKASEASRLAVTSAMESLSYHPNANARALAQQATETLGLVVGDVSDPFFGAMVKA
VEQVAYHTGNFLLIGNGYHNEQKERQAIEQLIRHRCAALVVHAKMIPDADLASLMKQIPGMVLINRILPGLEHRCVALDD
RYGAWLATRHLIQQGHTRIGYICSNHTISDAEDRLRGYYDALAESHIPANDRLVTFGEPDESGGEQAMTELLGRGRHFTA
VACYNDSMAAGAMGVLNDNGVGVPGEVSLIGFDDVLVSRYVRPRLTTIRYPIVTMATQAAELALALAGKCPTPEVTHVFS
PTLVRRHSVSTPTDTGHLSTTD

Sequences:

>Translated_342_residues
MATIKDVARLAGVSVATVSRVINDSPKASEASRLAVTSAMESLSYHPNANARALAQQATETLGLVVGDVSDPFFGAMVKA
VEQVAYHTGNFLLIGNGYHNEQKERQAIEQLIRHRCAALVVHAKMIPDADLASLMKQIPGMVLINRILPGLEHRCVALDD
RYGAWLATRHLIQQGHTRIGYICSNHTISDAEDRLRGYYDALAESHIPANDRLVTFGEPDESGGEQAMTELLGRGRHFTA
VACYNDSMAAGAMGVLNDNGVGVPGEVSLIGFDDVLVSRYVRPRLTTIRYPIVTMATQAAELALALAGKCPTPEVTHVFS
PTLVRRHSVSTPTDTGHLSTTD
>Mature_341_residues
ATIKDVARLAGVSVATVSRVINDSPKASEASRLAVTSAMESLSYHPNANARALAQQATETLGLVVGDVSDPFFGAMVKAV
EQVAYHTGNFLLIGNGYHNEQKERQAIEQLIRHRCAALVVHAKMIPDADLASLMKQIPGMVLINRILPGLEHRCVALDDR
YGAWLATRHLIQQGHTRIGYICSNHTISDAEDRLRGYYDALAESHIPANDRLVTFGEPDESGGEQAMTELLGRGRHFTAV
ACYNDSMAAGAMGVLNDNGVGVPGEVSLIGFDDVLVSRYVRPRLTTIRYPIVTMATQAAELALALAGKCPTPEVTHVFSP
TLVRRHSVSTPTDTGHLSTTD

Specific function: Repressor of the galactose operon. Binds galactose as an inducer [H]

COG id: COG1609

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789202, Length=343, Percent_Identity=90.6705539358601, Blast_Score=625, Evalue=1e-180,
Organism=Escherichia coli, GI1788474, Length=341, Percent_Identity=54.2521994134897, Blast_Score=367, Evalue=1e-103,
Organism=Escherichia coli, GI1789068, Length=338, Percent_Identity=34.3195266272189, Blast_Score=186, Evalue=2e-48,
Organism=Escherichia coli, GI1787948, Length=334, Percent_Identity=33.8323353293413, Blast_Score=174, Evalue=8e-45,
Organism=Escherichia coli, GI1790369, Length=304, Percent_Identity=33.5526315789474, Blast_Score=171, Evalue=7e-44,
Organism=Escherichia coli, GI1790194, Length=334, Percent_Identity=31.437125748503, Blast_Score=151, Evalue=8e-38,
Organism=Escherichia coli, GI1787580, Length=320, Percent_Identity=26.25, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1787906, Length=326, Percent_Identity=27.9141104294479, Blast_Score=97, Evalue=1e-21,
Organism=Escherichia coli, GI1786540, Length=342, Percent_Identity=26.3157894736842, Blast_Score=95, Evalue=7e-21,
Organism=Escherichia coli, GI1790715, Length=308, Percent_Identity=24.6753246753247, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI48994940, Length=321, Percent_Identity=23.3644859813084, Blast_Score=82, Evalue=5e-17,
Organism=Escherichia coli, GI1789456, Length=311, Percent_Identity=26.3665594855305, Blast_Score=78, Evalue=7e-16,
Organism=Escherichia coli, GI1786268, Length=128, Percent_Identity=32.03125, Blast_Score=63, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 36892; Mature: 36761

Theoretical pI: Translated: 6.63; Mature: 6.63

Prosite motif: PS00356 HTH_LACI_1 ; PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATIKDVARLAGVSVATVSRVINDSPKASEASRLAVTSAMESLSYHPNANARALAQQATE
CCCHHHHHHHHCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHH
TLGLVVGDVSDPFFGAMVKAVEQVAYHTGNFLLIGNGYHNEQKERQAIEQLIRHRCAALV
HHHEEEECCCCHHHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHH
VHAKMIPDADLASLMKQIPGMVLINRILPGLEHRCVALDDRYGAWLATRHLIQQGHTRIG
HHHHCCCCHHHHHHHHHCCHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHCCCEEE
YICSNHTISDAEDRLRGYYDALAESHIPANDRLVTFGEPDESGGEQAMTELLGRGRHFTA
EEECCCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCHHHHHHHHHHCCCCEEE
VACYNDSMAAGAMGVLNDNGVGVPGEVSLIGFDDVLVSRYVRPRLTTIRYPIVTMATQAA
EEEECCCCCCCEEEEECCCCCCCCCCEEEEEHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
ELALALAGKCPTPEVTHVFSPTLVRRHSVSTPTDTGHLSTTD
HHHHHHHCCCCCCCHHHHCCCHHHHHCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
ATIKDVARLAGVSVATVSRVINDSPKASEASRLAVTSAMESLSYHPNANARALAQQATE
CCHHHHHHHHCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHH
TLGLVVGDVSDPFFGAMVKAVEQVAYHTGNFLLIGNGYHNEQKERQAIEQLIRHRCAALV
HHHEEEECCCCHHHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHH
VHAKMIPDADLASLMKQIPGMVLINRILPGLEHRCVALDDRYGAWLATRHLIQQGHTRIG
HHHHCCCCHHHHHHHHHCCHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHCCCEEE
YICSNHTISDAEDRLRGYYDALAESHIPANDRLVTFGEPDESGGEQAMTELLGRGRHFTA
EEECCCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCHHHHHHHHHHCCCCEEE
VACYNDSMAAGAMGVLNDNGVGVPGEVSLIGFDDVLVSRYVRPRLTTIRYPIVTMATQAA
EEEECCCCCCCEEEEECCCCCCCCCCEEEEEHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
ELALALAGKCPTPEVTHVFSPTLVRRHSVSTPTDTGHLSTTD
HHHHHHHCCCCCCCHHHHCCCHHHHHCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 6283521; 9278503; 6350601; 8188660; 8982002 [H]