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The map label for this gene is hypB [H]

Identifier: 29143116

GI number: 29143116

Start: 2838971

End: 2839843

Strand: Direct

Name: hypB [H]

Synonym: t2757

Alternate gene names: 29143116

Gene position: 2838971-2839843 (Clockwise)

Preceding gene: 29143115

Following gene: 29143117

Centisome position: 59.24

GC content: 57.85

Gene sequence:

>873_bases
ATGTGTACGACTTGCGGTTGCGCTGAAGGCAACCTTTATATAGAAGGCGATGAACATAATCCGCACTCAGCGTTTCGTAG
CGCGCCCTTTGCCCCAGCGGCTCGCCCGGCGCTGAATATTACCGGCATTAAAACGCCTGACTTTACCCCAAGTCAGACCG
CAGAAGGCGATCTGCATTACGGCCACGGCGAAGCCGGAACCCACGCGCCTGGTATGAGTCAGCGGCGGATGCTGGAGGTT
GAAATCGACGTGCTGGACAAAAATAATCGTCTGGCTGAGCGCAACCGCGCCCGTTTCGCCGCCCGCCAGCAACTGGTGCT
CAACCTGGTGTCCAGCCCCGGGTCCGGTAAAACCACTCTGCTGACCGAAACGCTGATGAAATTGAAAGACCGTGTACCGT
GCGCGGTGATTGAAGGCGACCAGCAGACAGTGAACGACGCCGCCCGCATCCGCGCTACCGGCACGCCCGCCATTCAGGTC
AACACCGGTAAAGGCTGCCATCTTGACGCGCAAATGATCGCCGACGCCGCGCCGCGCCTGCCGCTGGATGACCACGGTAT
TCTGTTTATCGAAAACGTCGGAAATCTTGTCTGCCCGGCCAGCTTCGATCTGGGCGAAAAGCATAAAGTCGCAGTGCTTT
CCGTGACCGAAGGTGAAGATAAGCCGCTGAAGTATCCCCATATGTTTGCCGCGGCGTCGCTCATGCTGCTCAATAAAGTC
GATCTGCTGCCCTACCTCAATTTTGACGTTGAGAAGTGCATCGCCAGCGCGCGTGAAGTGAACCCGGAGATTGAAATTAT
CCTGATTTCCGCCACCAGCGGCGAAGGCATGGATCAGTGGCTGGCCTGGCTGGAGGCGCAGCGATGTGCATAG

Upstream 100 bases:

>100_bases
GCGCATAGAGATTGATGAAACTGAAGATTAATGCCGGATAAGACGCTCGCGTCGCCATCCGGCAGAAAGCCTGTAACAAG
AAGAAGATCAGGAGTAAGCG

Downstream 100 bases:

>100_bases
GCGTTCCGGGTCAAATCCGCGCCATTGACGGCAATCAGGCAAAAGTTGACGTCTGCGGCATTCAGCGCGACGTCGATCTC
ACATTAGTCGGCAGTTGTGA

Product: hydrogenase nickel incorporation protein HypB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 290; Mature: 290

Protein sequence:

>290_residues
MCTTCGCAEGNLYIEGDEHNPHSAFRSAPFAPAARPALNITGIKTPDFTPSQTAEGDLHYGHGEAGTHAPGMSQRRMLEV
EIDVLDKNNRLAERNRARFAARQQLVLNLVSSPGSGKTTLLTETLMKLKDRVPCAVIEGDQQTVNDAARIRATGTPAIQV
NTGKGCHLDAQMIADAAPRLPLDDHGILFIENVGNLVCPASFDLGEKHKVAVLSVTEGEDKPLKYPHMFAAASLMLLNKV
DLLPYLNFDVEKCIASAREVNPEIEIILISATSGEGMDQWLAWLEAQRCA

Sequences:

>Translated_290_residues
MCTTCGCAEGNLYIEGDEHNPHSAFRSAPFAPAARPALNITGIKTPDFTPSQTAEGDLHYGHGEAGTHAPGMSQRRMLEV
EIDVLDKNNRLAERNRARFAARQQLVLNLVSSPGSGKTTLLTETLMKLKDRVPCAVIEGDQQTVNDAARIRATGTPAIQV
NTGKGCHLDAQMIADAAPRLPLDDHGILFIENVGNLVCPASFDLGEKHKVAVLSVTEGEDKPLKYPHMFAAASLMLLNKV
DLLPYLNFDVEKCIASAREVNPEIEIILISATSGEGMDQWLAWLEAQRCA
>Mature_290_residues
MCTTCGCAEGNLYIEGDEHNPHSAFRSAPFAPAARPALNITGIKTPDFTPSQTAEGDLHYGHGEAGTHAPGMSQRRMLEV
EIDVLDKNNRLAERNRARFAARQQLVLNLVSSPGSGKTTLLTETLMKLKDRVPCAVIEGDQQTVNDAARIRATGTPAIQV
NTGKGCHLDAQMIADAAPRLPLDDHGILFIENVGNLVCPASFDLGEKHKVAVLSVTEGEDKPLKYPHMFAAASLMLLNKV
DLLPYLNFDVEKCIASAREVNPEIEIILISATSGEGMDQWLAWLEAQRCA

Specific function: Required for the maturation of the three [NiFe]hydrogenases. Exhibits a low intrinsic GTPase activity. The GTP hydrolysis catalyzed by HypB is an integral process in the incorporation of nickel into hydrogenases [H]

COG id: COG0378

COG function: function code OK; Ni2+-binding GTPase involved in regulation of expression and maturation of urease and hydrogenase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hypB/hupM family [H]

Homologues:

Organism=Escherichia coli, GI1789082, Length=290, Percent_Identity=95.1724137931034, Blast_Score=577, Evalue=1e-166,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003495
- InterPro:   IPR004392
- InterPro:   IPR012202 [H]

Pfam domain/function: PF02492 cobW [H]

EC number: NA

Molecular weight: Translated: 31423; Mature: 31423

Theoretical pI: Translated: 5.36; Mature: 5.36

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCTTCGCAEGNLYIEGDEHNPHSAFRSAPFAPAARPALNITGIKTPDFTPSQTAEGDLHY
CCCCCCCCCCCEEEECCCCCHHHHHHCCCCCCCCCCCEEEECCCCCCCCCCCCCCCCEEE
GHGEAGTHAPGMSQRRMLEVEIDVLDKNNRLAERNRARFAARQQLVLNLVSSPGSGKTTL
CCCCCCCCCCCCCCCEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHH
LTETLMKLKDRVPCAVIEGDQQTVNDAARIRATGTPAIQVNTGKGCHLDAQMIADAAPRL
HHHHHHHHHHCCCEEEEECCHHHHCCHHEEEECCCCEEEEECCCCCEEHHHHHHCCCCCC
PLDDHGILFIENVGNLVCPASFDLGEKHKVAVLSVTEGEDKPLKYPHMFAAASLMLLNKV
CCCCCCEEEEECCCCEECCCCCCCCCCCEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHH
DLLPYLNFDVEKCIASAREVNPEIEIILISATSGEGMDQWLAWLEAQRCA
HHCCCCCCCHHHHHHHHHHCCCCEEEEEEECCCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MCTTCGCAEGNLYIEGDEHNPHSAFRSAPFAPAARPALNITGIKTPDFTPSQTAEGDLHY
CCCCCCCCCCCEEEECCCCCHHHHHHCCCCCCCCCCCEEEECCCCCCCCCCCCCCCCEEE
GHGEAGTHAPGMSQRRMLEVEIDVLDKNNRLAERNRARFAARQQLVLNLVSSPGSGKTTL
CCCCCCCCCCCCCCCEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHH
LTETLMKLKDRVPCAVIEGDQQTVNDAARIRATGTPAIQVNTGKGCHLDAQMIADAAPRL
HHHHHHHHHHCCCEEEEECCHHHHCCHHEEEECCCCEEEEECCCCCEEHHHHHHCCCCCC
PLDDHGILFIENVGNLVCPASFDLGEKHKVAVLSVTEGEDKPLKYPHMFAAASLMLLNKV
CCCCCCEEEEECCCCEECCCCCCCCCCCEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHH
DLLPYLNFDVEKCIASAREVNPEIEIILISATSGEGMDQWLAWLEAQRCA
HHCCCCCCCHHHHHHHHHHCCCCEEEEEEECCCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]