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The map label for this gene is mdfA [H]

Identifier: 29142451

GI number: 29142451

Start: 2086937

End: 2088169

Strand: Reverse

Name: mdfA [H]

Synonym: t2030

Alternate gene names: 29142451

Gene position: 2088169-2086937 (Counterclockwise)

Preceding gene: 29142454

Following gene: 29142448

Centisome position: 43.58

GC content: 54.91

Gene sequence:

>1233_bases
ATGCAGAATCGTTTACAATCAGGCGGCAGGCTGGGACGTCAGGCGCTGCTTTTTCCTCTCTGTCTGGTGCTGTACGAGTT
TTCTACTTATATTGGCAACGACATGATCCAACCGGGTATGCTGGCGGTGGTTGAGCAGTATCAGGCGGGCCTGGACTGGG
TTCCGACGTCCATGACCGCTTATCTGGCGGGCGGTATGTTCTTACAATGGCTGCTTGGCCCGTTGTCTGACCGGATTGGC
CGTCGTCCCGTGATGCTGGCGGGCGTCGTGTGGTTTATTGTCACCTGCCTGGCGACGTTACTGGCGAAAAACATCGAACA
ATTTACATTCCTGCGTTTTTTGCAAGGGATTAGCCTGTGTTTTATCGGCGCTGTAGGGTATGCCGCGATTCAGGAATCCT
TTGAGGAGGCGGTGTGTATAAAAATTACCGCGCTGATGGCGAACGTGGCGTTAATCGCGCCGCTACTGGGACCACTAGTT
GGGGCGGCATGGGTGCATGTTCTGCCGTGGGAGGGGATGTTTATTTTATTTGCCGCACTGGCCGCTATCGCCTTTTTCGG
GCTACAGCGCGCAATGCCGGAGACCGCCACGCGGCGGGGCGAAACGCTGTCATTTAAGGCGCTTGGCCGGGATTATCGGC
TGGTGATAAAAAATCGGCGGTTCGTCGCAGGGGCGTTAGCGCTGGGATTTGTCAGCCTGCCGCTGTTGGCCTGGATTGCG
CAGTCGCCGATTATCATCATTAGCGGCGAACAGCTCAGCAGCTATGAATATGGTTTGCTGCAGGTGCCCGTTTTTGGCGC
GCTCATTGCCGGTAATCTGGTGTTGGCGCGTTTAACCTCCCGACGTACGGTTCGCTCGCTGATCGTGATGGGCGGCTGGC
CTATCGTCGCGGGGCTGATCATCGCCGCGGCGGCGACGGTGGTTTCATCCCATGCTTATTTATGGATGACGGCGGGATTA
AGCGTATACGCTTTTGGCATTGGGCTGGCGAATGCCGGACTGGTGCGTCTGACGCTCTTTTCCAGCGATATGAGTAAAGG
AACGGTCTCGGCGGCGATGGGAATGCTACAAATGCTGATTTTTACCGTTGGCATTGAAGTCAGTAAACACGCCTGGTTAA
GCGGCGGCAATGGGCTATTTAGCCTGTTTAATCTGGCAAACGGTATTCTTTGGCTACTGCTGATGCTGGTCTTCTTAAAA
GATAAGCGGACGGGGAATTTGCAAACAGTCTGA

Upstream 100 bases:

>100_bases
GTAAAAATGTGCGCTATTTCCCTTGCAGCCTCATAATTCGCTATCTCAGTTCCCCGACTTTCATTACACTCTGCGCGGTT
TTTATTGGCGAAGAGATGGT

Downstream 100 bases:

>100_bases
GAGAGCGGCTACGCCTGATAAGCTTTCTGTTATCAGGCGTAGAGAGGCATCCGCTAGACGTTGAACGGCGGCTCGTTGTT
TAATACGCTGTCGATGATAT

Product: multidrug translocase MdfA

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: Chloramphenicol resistance pump cmr [H]

Number of amino acids: Translated: 410; Mature: 410

Protein sequence:

>410_residues
MQNRLQSGGRLGRQALLFPLCLVLYEFSTYIGNDMIQPGMLAVVEQYQAGLDWVPTSMTAYLAGGMFLQWLLGPLSDRIG
RRPVMLAGVVWFIVTCLATLLAKNIEQFTFLRFLQGISLCFIGAVGYAAIQESFEEAVCIKITALMANVALIAPLLGPLV
GAAWVHVLPWEGMFILFAALAAIAFFGLQRAMPETATRRGETLSFKALGRDYRLVIKNRRFVAGALALGFVSLPLLAWIA
QSPIIIISGEQLSSYEYGLLQVPVFGALIAGNLVLARLTSRRTVRSLIVMGGWPIVAGLIIAAAATVVSSHAYLWMTAGL
SVYAFGIGLANAGLVRLTLFSSDMSKGTVSAAMGMLQMLIFTVGIEVSKHAWLSGGNGLFSLFNLANGILWLLLMLVFLK
DKRTGNLQTV

Sequences:

>Translated_410_residues
MQNRLQSGGRLGRQALLFPLCLVLYEFSTYIGNDMIQPGMLAVVEQYQAGLDWVPTSMTAYLAGGMFLQWLLGPLSDRIG
RRPVMLAGVVWFIVTCLATLLAKNIEQFTFLRFLQGISLCFIGAVGYAAIQESFEEAVCIKITALMANVALIAPLLGPLV
GAAWVHVLPWEGMFILFAALAAIAFFGLQRAMPETATRRGETLSFKALGRDYRLVIKNRRFVAGALALGFVSLPLLAWIA
QSPIIIISGEQLSSYEYGLLQVPVFGALIAGNLVLARLTSRRTVRSLIVMGGWPIVAGLIIAAAATVVSSHAYLWMTAGL
SVYAFGIGLANAGLVRLTLFSSDMSKGTVSAAMGMLQMLIFTVGIEVSKHAWLSGGNGLFSLFNLANGILWLLLMLVFLK
DKRTGNLQTV
>Mature_410_residues
MQNRLQSGGRLGRQALLFPLCLVLYEFSTYIGNDMIQPGMLAVVEQYQAGLDWVPTSMTAYLAGGMFLQWLLGPLSDRIG
RRPVMLAGVVWFIVTCLATLLAKNIEQFTFLRFLQGISLCFIGAVGYAAIQESFEEAVCIKITALMANVALIAPLLGPLV
GAAWVHVLPWEGMFILFAALAAIAFFGLQRAMPETATRRGETLSFKALGRDYRLVIKNRRFVAGALALGFVSLPLLAWIA
QSPIIIISGEQLSSYEYGLLQVPVFGALIAGNLVLARLTSRRTVRSLIVMGGWPIVAGLIIAAAATVVSSHAYLWMTAGL
SVYAFGIGLANAGLVRLTLFSSDMSKGTVSAAMGMLQMLIFTVGIEVSKHAWLSGGNGLFSLFNLANGILWLLLMLVFLK
DKRTGNLQTV

Specific function: Efflux pump driven by the proton motive force. Confers resistance to basic, aromatic and lipophilic drugs as well as neutral, non-aromatic, zwitterionic or non-aromatic hydrophilic antibiotics [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily [H]

Homologues:

Organism=Escherichia coli, GI1787065, Length=406, Percent_Identity=90.8866995073892, Blast_Score=752, Evalue=0.0,
Organism=Escherichia coli, GI1790794, Length=379, Percent_Identity=41.688654353562, Blast_Score=312, Evalue=2e-86,
Organism=Escherichia coli, GI48994889, Length=217, Percent_Identity=25.3456221198157, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1788509, Length=303, Percent_Identity=23.1023102310231, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1790146, Length=233, Percent_Identity=23.175965665236, Blast_Score=66, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR005829 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 44349; Mature: 44349

Theoretical pI: Translated: 9.95; Mature: 9.95

Prosite motif: PS50850 MFS ; PS00216 SUGAR_TRANSPORT_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHCCHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure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HHHCCHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]