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The map label for this gene is pgk

Identifier: 28377643

GI number: 28377643

Start: 726122

End: 727324

Strand: Direct

Name: pgk

Synonym: lp_0790

Alternate gene names: 28377643

Gene position: 726122-727324 (Clockwise)

Preceding gene: 28377642

Following gene: 28377644

Centisome position: 21.95

GC content: 43.23

Gene sequence:

>1203_bases
TTGGCTAAATTAATCGTTTCAGATTTAGATGTTAAAGATAAAAAAGTTTTAATTCGTGTCGACTTTAATGTTCCAATTAA
AGACGGCAAAATTGGTGACGACAACCGGATCGTCGCTGCCTTACCAACGATCAAATACGTGATTGAACATGATGGCAAAG
CCATCTTATTCTCACATTTAGGTCGGATTAAGAGCGAAGACGACAAGAAGGGCTTAAGCTTACGTCCTGTTGCAGAACGT
CTTTCAAACTTATTAAACAAGCCTGTTACCTTCGTCCCTGTTACTGAAGGCGAACAACTTGAAACTGCCATCAACAACAT
GAACGACGGTGACGTCTTAGTTGTTGAAAACACTCGTTTTGAAGACGTTGTTAACGGCGAACAAGTTAAGCGCGAATCTG
GTAACGATCCTGAATTAGGCAAGTACTGGGCTTCACTTGGTGATGTCTACGTCAACGATGCTTTCGGGACTGCTCACCGG
AGCCATGCTTCTAACGTTGGGATTGCTTCAAACATGGATCAAGTTGCCGCTGGTTTCTTAATGGAAAAGGAAATCAAGTT
CTTAGGTGACGCGGTTGACAATCCTAAGCACCCATTCGTTGCTATCTTAGGTGGTGCCAAGGTTTCTGATAAGATCGGTG
TTATCGATCACTTAATCAGCAAAGCTGACAAGATCATCATCGGTGGTGGGATGACTTATACGTTCTATGCTGCTAAAGGC
ATGGGCATTGGTAACTCACTTGTTGAAAAAGACAAGATTGAATTGGCTAAGTCAATCATCGAAAAAGCTGGCGACAAGTT
AGTCTTACCTAGCGACTCAATCGTTGCTGAAAAGTTTGATAACGATGTTCCTAGCAAGGTTGTTGAAGGTTCAATTCCTG
ATGGCTACATGGCCTTAGATATTGGTCCTAAGTCAATCGAAGAATTCGAAGATGTTTTACGCGACGCTAAGACCGTTGTT
TGGAACGGACCAATGGGTGTCTTCGAAATGAGCAACTACGCCAAGGGTACGCTTGAAATTGGTAAGTTCTTAGGAACCTT
ATCAGACGCAACGACCATCGTTGGTGGTGGGGACTCAACTGCCGCTGTTAAGCAACTTGGCGTTGGCGACAAGTTAACCC
ACATTTCTACTGGTGGCGGTGCTTCACTTGAATACCTTGAAGGTAAGGAATTACCTGGTATTGCTGCTATTTCTGAAAAA
TAA

Upstream 100 bases:

>100_bases
AACTTTAAAACCGCATATGGTTTTGACGGCGGGGGGAGGCAAAACTCCTCTTGCCGTTTTTTTAATTTCAGCAGTCACGA
ATTCTAGGAGGATTTTAACA

Downstream 100 bases:

>100_bases
TCTGTTCTCCGAAAGGAAGGTTTATCGTGCGTACACCTATTATTGCCGGTAACTGGAAAATGAACAAAACAGCTAGCGAA
GCTTTAGCTTTCGTTAATGC

Product: phosphoglycerate kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 400; Mature: 399

Protein sequence:

>400_residues
MAKLIVSDLDVKDKKVLIRVDFNVPIKDGKIGDDNRIVAALPTIKYVIEHDGKAILFSHLGRIKSEDDKKGLSLRPVAER
LSNLLNKPVTFVPVTEGEQLETAINNMNDGDVLVVENTRFEDVVNGEQVKRESGNDPELGKYWASLGDVYVNDAFGTAHR
SHASNVGIASNMDQVAAGFLMEKEIKFLGDAVDNPKHPFVAILGGAKVSDKIGVIDHLISKADKIIIGGGMTYTFYAAKG
MGIGNSLVEKDKIELAKSIIEKAGDKLVLPSDSIVAEKFDNDVPSKVVEGSIPDGYMALDIGPKSIEEFEDVLRDAKTVV
WNGPMGVFEMSNYAKGTLEIGKFLGTLSDATTIVGGGDSTAAVKQLGVGDKLTHISTGGGASLEYLEGKELPGIAAISEK

Sequences:

>Translated_400_residues
MAKLIVSDLDVKDKKVLIRVDFNVPIKDGKIGDDNRIVAALPTIKYVIEHDGKAILFSHLGRIKSEDDKKGLSLRPVAER
LSNLLNKPVTFVPVTEGEQLETAINNMNDGDVLVVENTRFEDVVNGEQVKRESGNDPELGKYWASLGDVYVNDAFGTAHR
SHASNVGIASNMDQVAAGFLMEKEIKFLGDAVDNPKHPFVAILGGAKVSDKIGVIDHLISKADKIIIGGGMTYTFYAAKG
MGIGNSLVEKDKIELAKSIIEKAGDKLVLPSDSIVAEKFDNDVPSKVVEGSIPDGYMALDIGPKSIEEFEDVLRDAKTVV
WNGPMGVFEMSNYAKGTLEIGKFLGTLSDATTIVGGGDSTAAVKQLGVGDKLTHISTGGGASLEYLEGKELPGIAAISEK
>Mature_399_residues
AKLIVSDLDVKDKKVLIRVDFNVPIKDGKIGDDNRIVAALPTIKYVIEHDGKAILFSHLGRIKSEDDKKGLSLRPVAERL
SNLLNKPVTFVPVTEGEQLETAINNMNDGDVLVVENTRFEDVVNGEQVKRESGNDPELGKYWASLGDVYVNDAFGTAHRS
HASNVGIASNMDQVAAGFLMEKEIKFLGDAVDNPKHPFVAILGGAKVSDKIGVIDHLISKADKIIIGGGMTYTFYAAKGM
GIGNSLVEKDKIELAKSIIEKAGDKLVLPSDSIVAEKFDNDVPSKVVEGSIPDGYMALDIGPKSIEEFEDVLRDAKTVVW
NGPMGVFEMSNYAKGTLEIGKFLGTLSDATTIVGGGDSTAAVKQLGVGDKLTHISTGGGASLEYLEGKELPGIAAISEK

Specific function: Second phase of glycolysis; second step. [C]

COG id: COG0126

COG function: function code G; 3-phosphoglycerate kinase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoglycerate kinase family

Homologues:

Organism=Homo sapiens, GI4505763, Length=412, Percent_Identity=43.2038834951456, Blast_Score=323, Evalue=2e-88,
Organism=Homo sapiens, GI31543397, Length=414, Percent_Identity=43.2367149758454, Blast_Score=313, Evalue=3e-85,
Organism=Escherichia coli, GI1789294, Length=401, Percent_Identity=41.1471321695761, Blast_Score=291, Evalue=6e-80,
Organism=Caenorhabditis elegans, GI17508823, Length=414, Percent_Identity=45.1690821256039, Blast_Score=339, Evalue=2e-93,
Organism=Saccharomyces cerevisiae, GI6319857, Length=415, Percent_Identity=49.1566265060241, Blast_Score=353, Evalue=3e-98,
Organism=Drosophila melanogaster, GI17136394, Length=412, Percent_Identity=44.4174757281553, Blast_Score=318, Evalue=4e-87,
Organism=Drosophila melanogaster, GI24581203, Length=415, Percent_Identity=43.3734939759036, Blast_Score=306, Evalue=1e-83,

Paralogues:

None

Copy number: 2990 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1460 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). [C]

Swissprot (AC and ID): PGK_LACPL (Q88YH5)

Other databases:

- EMBL:   AL935254
- RefSeq:   NP_784535.1
- ProteinModelPortal:   Q88YH5
- SMR:   Q88YH5
- GeneID:   1063805
- GenomeReviews:   AL935263_GR
- KEGG:   lpl:lp_0790
- NMPDR:   fig|220668.1.peg.669
- HOGENOM:   HBG453500
- OMA:   DCIGDEV
- ProtClustDB:   PRK00073
- BioCyc:   LPLA220668:LP_0790-MONOMER
- BRENDA:   2.7.2.3
- GO:   GO:0005737
- GO:   GO:0006096
- HAMAP:   MF_00145
- InterPro:   IPR001576
- InterPro:   IPR015901
- InterPro:   IPR015911
- InterPro:   IPR015824
- Gene3D:   G3DSA:3.40.50.1270
- Gene3D:   G3DSA:3.40.50.1260
- PANTHER:   PTHR11406
- PIRSF:   PIRSF000724
- PRINTS:   PR00477

Pfam domain/function: PF00162 PGK; SSF53748 PGK

EC number: =2.7.2.3

Molecular weight: Translated: 42797; Mature: 42666

Theoretical pI: Translated: 4.81; Mature: 4.81

Prosite motif: PS00111 PGLYCERATE_KINASE

Important sites: BINDING 36-36 BINDING 119-119 BINDING 160-160 BINDING 211-211 BINDING 329-329

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKLIVSDLDVKDKKVLIRVDFNVPIKDGKIGDDNRIVAALPTIKYVIEHDGKAILFSHL
CCCEEEECCCCCCCEEEEEEECCCCCCCCCCCCCCEEEEECCCEEEEECCCCCEEHHHHH
GRIKSEDDKKGLSLRPVAERLSNLLNKPVTFVPVTEGEQLETAINNMNDGDVLVVENTRF
CCCCCCCCCCCCEECHHHHHHHHHHHCCEEEEECCCCCHHHHHHCCCCCCCEEEEECCCH
EDVVNGEQVKRESGNDPELGKYWASLGDVYVNDAFGTAHRSHASNVGIASNMDQVAAGFL
HHHHCCHHHHHCCCCCCHHHHHHHHHCCEEEECCCCCHHHCCCCCCCCCCCHHHHHHHHH
MEKEIKFLGDAVDNPKHPFVAILGGAKVSDKIGVIDHLISKADKIIIGGGMTYTFYAAKG
HHHHHHHHHHHCCCCCCCEEEEECCCCCCCHHHHHHHHHHCCCEEEECCCCEEEEEEECC
MGIGNSLVEKDKIELAKSIIEKAGDKLVLPSDSIVAEKFDNDVPSKVVEGSIPDGYMALD
CCCCHHHHHHHHHHHHHHHHHHCCCEEECCCCCHHHHHHCCCCCHHHHCCCCCCCEEEEE
IGPKSIEEFEDVLRDAKTVVWNGPMGVFEMSNYAKGTLEIGKFLGTLSDATTIVGGGDST
CCHHHHHHHHHHHHCCCEEEECCCCCEEECCCCCCCHHHHHHHHHHHCCCCEEEECCCCH
AAVKQLGVGDKLTHISTGGGASLEYLEGKELPGIAAISEK
HHHHHCCCCCCEEEEECCCCCEEEEECCCCCCCCCCCCCC
>Mature Secondary Structure 
AKLIVSDLDVKDKKVLIRVDFNVPIKDGKIGDDNRIVAALPTIKYVIEHDGKAILFSHL
CCEEEECCCCCCCEEEEEEECCCCCCCCCCCCCCEEEEECCCEEEEECCCCCEEHHHHH
GRIKSEDDKKGLSLRPVAERLSNLLNKPVTFVPVTEGEQLETAINNMNDGDVLVVENTRF
CCCCCCCCCCCCEECHHHHHHHHHHHCCEEEEECCCCCHHHHHHCCCCCCCEEEEECCCH
EDVVNGEQVKRESGNDPELGKYWASLGDVYVNDAFGTAHRSHASNVGIASNMDQVAAGFL
HHHHCCHHHHHCCCCCCHHHHHHHHHCCEEEECCCCCHHHCCCCCCCCCCCHHHHHHHHH
MEKEIKFLGDAVDNPKHPFVAILGGAKVSDKIGVIDHLISKADKIIIGGGMTYTFYAAKG
HHHHHHHHHHHCCCCCCCEEEEECCCCCCCHHHHHHHHHHCCCEEEECCCCEEEEEEECC
MGIGNSLVEKDKIELAKSIIEKAGDKLVLPSDSIVAEKFDNDVPSKVVEGSIPDGYMALD
CCCCHHHHHHHHHHHHHHHHHHCCCEEECCCCCHHHHHHCCCCCHHHHCCCCCCCEEEEE
IGPKSIEEFEDVLRDAKTVVWNGPMGVFEMSNYAKGTLEIGKFLGTLSDATTIVGGGDST
CCHHHHHHHHHHHHCCCEEEECCCCCEEECCCCCCCHHHHHHHHHHHCCCCEEEECCCCH
AAVKQLGVGDKLTHISTGGGASLEYLEGKELPGIAAISEK
HHHHHCCCCCCEEEEECCCCCEEEEECCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12566566