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The map label for this gene is tpiA

Identifier: 28377644

GI number: 28377644

Start: 727351

End: 728109

Strand: Direct

Name: tpiA

Synonym: lp_0791

Alternate gene names: 28377644

Gene position: 727351-728109 (Clockwise)

Preceding gene: 28377643

Following gene: 28377645

Centisome position: 21.99

GC content: 42.56

Gene sequence:

>759_bases
GTGCGTACACCTATTATTGCCGGTAACTGGAAAATGAACAAAACAGCTAGCGAAGCTTTAGCTTTCGTTAATGCTGTTAA
GGATCAATTACCAGATCCATCAAAAGTTGAATCAGTCGTTGCTGCCCCAGCCCTTTTCTTACAGGAAATGGTTGAAGCTG
CCAAGGGTTCTGATTTAAAGATTGCTGCTGAAAATGCTTATTTTGAAGATGCTGGGGCATTTACTGGTGAAACTTCACCA
GCTGCCTTAGCTGACTTAGGCGTTGATTACGTTGTTATTGGTCATTCAGAACGTCGCGGTTATTTCCACGAAACTGACGA
AGACATTAACAAAAAGGCACACGCTATCTTCAAGAACGGCATGAAGCCAATCATTTGCTGTGGCGAATCATTAGAACAAC
GTGAAGCCGGCGAAGCAGAATCATGGGTTTCAGGCCAAATCAAGGCTGCTTTGAAAGACTTATCAGCAGACCAAGTAAGT
TCATTGGTTATTGCCTACGAACCAATCTGGGCCATCGGTACTGGTAAGACTGCTACGAGTGACCAAGCTGAAGAAATTTG
TGCGGTTGTTCGTAAGACTGTTGCTGACCTTTACTCACAAGAAGTTGCTGATAAAGTTCGGATTCAATACGGTGGTAGTG
TTAAACCAGCCAACGTTAACGAATTAATGGGCAAAGATGATATCGACGGTGGTTTAGTCGGTGGTGCTTCATTACAACCT
GATTCATTCTTGGAACTTGTTAATTATCAAAATAACTAA

Upstream 100 bases:

>100_bases
CTGGTGGCGGTGCTTCACTTGAATACCTTGAAGGTAAGGAATTACCTGGTATTGCTGCTATTTCTGAAAAATAATCTGTT
CTCCGAAAGGAAGGTTTATC

Downstream 100 bases:

>100_bases
TAATAGTTATTGCAAGTTGATTGACAAACTTCTACAATAAGTGTGAAACAAATAACAAGCCAATTTTAAGGAGAGAAATT
AATGTCTATTATTACAGATA

Product: triosephosphate isomerase

Products: NA

Alternate protein names: TIM; Triose-phosphate isomerase

Number of amino acids: Translated: 252; Mature: 252

Protein sequence:

>252_residues
MRTPIIAGNWKMNKTASEALAFVNAVKDQLPDPSKVESVVAAPALFLQEMVEAAKGSDLKIAAENAYFEDAGAFTGETSP
AALADLGVDYVVIGHSERRGYFHETDEDINKKAHAIFKNGMKPIICCGESLEQREAGEAESWVSGQIKAALKDLSADQVS
SLVIAYEPIWAIGTGKTATSDQAEEICAVVRKTVADLYSQEVADKVRIQYGGSVKPANVNELMGKDDIDGGLVGGASLQP
DSFLELVNYQNN

Sequences:

>Translated_252_residues
MRTPIIAGNWKMNKTASEALAFVNAVKDQLPDPSKVESVVAAPALFLQEMVEAAKGSDLKIAAENAYFEDAGAFTGETSP
AALADLGVDYVVIGHSERRGYFHETDEDINKKAHAIFKNGMKPIICCGESLEQREAGEAESWVSGQIKAALKDLSADQVS
SLVIAYEPIWAIGTGKTATSDQAEEICAVVRKTVADLYSQEVADKVRIQYGGSVKPANVNELMGKDDIDGGLVGGASLQP
DSFLELVNYQNN
>Mature_252_residues
MRTPIIAGNWKMNKTASEALAFVNAVKDQLPDPSKVESVVAAPALFLQEMVEAAKGSDLKIAAENAYFEDAGAFTGETSP
AALADLGVDYVVIGHSERRGYFHETDEDINKKAHAIFKNGMKPIICCGESLEQREAGEAESWVSGQIKAALKDLSADQVS
SLVIAYEPIWAIGTGKTATSDQAEEICAVVRKTVADLYSQEVADKVRIQYGGSVKPANVNELMGKDDIDGGLVGGASLQP
DSFLELVNYQNN

Specific function: Plays an important role in several metabolic pathways. [C]

COG id: COG0149

COG function: function code G; Triosephosphate isomerase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the triosephosphate isomerase family

Homologues:

Organism=Homo sapiens, GI4507645, Length=253, Percent_Identity=42.2924901185771, Blast_Score=175, Evalue=4e-44,
Organism=Homo sapiens, GI226529917, Length=253, Percent_Identity=42.2924901185771, Blast_Score=175, Evalue=4e-44,
Organism=Escherichia coli, GI1790353, Length=247, Percent_Identity=42.1052631578947, Blast_Score=202, Evalue=2e-53,
Organism=Caenorhabditis elegans, GI17536593, Length=254, Percent_Identity=44.4881889763779, Blast_Score=186, Evalue=7e-48,
Organism=Saccharomyces cerevisiae, GI6320255, Length=250, Percent_Identity=39.2, Blast_Score=182, Evalue=3e-47,
Organism=Drosophila melanogaster, GI28572008, Length=247, Percent_Identity=44.9392712550607, Blast_Score=202, Evalue=2e-52,
Organism=Drosophila melanogaster, GI28572006, Length=247, Percent_Identity=44.9392712550607, Blast_Score=202, Evalue=2e-52,
Organism=Drosophila melanogaster, GI28572004, Length=247, Percent_Identity=44.9392712550607, Blast_Score=202, Evalue=2e-52,

Paralogues:

None

Copy number: 1120 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 60 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): TPIS_LACPL (Q88YH4)

Other databases:

- EMBL:   AL935254
- RefSeq:   NP_784536.1
- ProteinModelPortal:   Q88YH4
- SMR:   Q88YH4
- GeneID:   1063807
- GenomeReviews:   AL935263_GR
- KEGG:   lpl:lp_0791
- NMPDR:   fig|220668.1.peg.670
- HOGENOM:   HBG708281
- OMA:   DIRSVQT
- ProtClustDB:   PRK00042
- BioCyc:   LPLA220668:LP_0791-MONOMER
- BRENDA:   5.3.1.1
- GO:   GO:0005737
- GO:   GO:0006094
- GO:   GO:0006096
- HAMAP:   MF_00147_B
- InterPro:   IPR013785
- InterPro:   IPR022896
- InterPro:   IPR000652
- InterPro:   IPR020861
- Gene3D:   G3DSA:3.20.20.70
- PANTHER:   PTHR21139
- TIGRFAMs:   TIGR00419

Pfam domain/function: PF00121 TIM; SSF51351 Triophos_ismrse

EC number: =5.3.1.1

Molecular weight: Translated: 26971; Mature: 26971

Theoretical pI: Translated: 4.36; Mature: 4.36

Prosite motif: PS00171 TIM_1; PS51440 TIM_2

Important sites: ACT_SITE 95-95 ACT_SITE 167-167 BINDING 9-9 BINDING 11-11

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRTPIIAGNWKMNKTASEALAFVNAVKDQLPDPSKVESVVAAPALFLQEMVEAAKGSDLK
CCCCEECCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEE
IAAENAYFEDAGAFTGETSPAALADLGVDYVVIGHSERRGYFHETDEDINKKAHAIFKNG
EEECCCEECCCCCCCCCCCCHHHHHCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHCC
MKPIICCGESLEQREAGEAESWVSGQIKAALKDLSADQVSSLVIAYEPIWAIGTGKTATS
CCEEEECCCHHHHHHCCCHHHHHHHHHHHHHHHCCHHHHHHEEEEECCEEEEECCCCCCC
DQAEEICAVVRKTVADLYSQEVADKVRIQYGGSVKPANVNELMGKDDIDGGLVGGASLQP
HHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCHHHHCCCCCCCCCEECCCCCCH
DSFLELVNYQNN
HHHHHHHHCCCC
>Mature Secondary Structure
MRTPIIAGNWKMNKTASEALAFVNAVKDQLPDPSKVESVVAAPALFLQEMVEAAKGSDLK
CCCCEECCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEE
IAAENAYFEDAGAFTGETSPAALADLGVDYVVIGHSERRGYFHETDEDINKKAHAIFKNG
EEECCCEECCCCCCCCCCCCHHHHHCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHCC
MKPIICCGESLEQREAGEAESWVSGQIKAALKDLSADQVSSLVIAYEPIWAIGTGKTATS
CCEEEECCCHHHHHHCCCHHHHHHHHHHHHHHHCCHHHHHHEEEEECCEEEEECCCCCCC
DQAEEICAVVRKTVADLYSQEVADKVRIQYGGSVKPANVNELMGKDDIDGGLVGGASLQP
HHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCHHHHCCCCCCCCCEECCCCCCH
DSFLELVNYQNN
HHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12566566