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The map label for this gene is lysS

Identifier: 28377434

GI number: 28377434

Start: 500766

End: 502265

Strand: Direct

Name: lysS

Synonym: lp_0550

Alternate gene names: 28377434

Gene position: 500766-502265 (Clockwise)

Preceding gene: 28377433

Following gene: 28377435

Centisome position: 15.14

GC content: 43.67

Gene sequence:

>1500_bases
GTGGCACGACAAGAACAGACGATGAACGACCAACTGAAGGTTCGTCGCGAAAAAATGGATGAACTGCGTGAAGAGGGGAT
TGATCCCTTTGGTCACCGGTTCGAACGGACTGATTTAGCCCAGGACTTGCAAGACAAGTATGGGGATATGGATAAGGACG
AGCTAGACGCTAAACAAGTGGTGGCAACCATTGCTGGACGGATGCTAGCTAAGCGTGGTAAGGGTAAAGTCGGTTTTGCT
GATATCTGGGATCGCTCAGGTAAGATGCAGTTATACATTCGTAAAGATGTAGTCGGCGAAGATACTTACCATATCTTCAA
GCGTTCTGATATTGGTGATTTCCTTGGAATTACCGGCCAAGTTTTTAAGACGGACTTTGGCGAATTAACGATTAAAGTAA
CTGGTTTAACGTTCTTATCAAAGGCGTTACGACCATTGCCAGATAAGTTCCATGGGTTACAGAATGTGGAACAGATCTAT
CGCCAACGGTATTTGGACTTGATCTCTAACCGCGATAGCTTTGATCGTTTCTTGAAGCGGACGAAGATCATTTCAGCGAT
TCGGCATCATTTAGACGATCAAGGCTTCACAGAAGTTGAGACGCCAATGTTGCATAATCAAGCTGGTGGTGCGGCAGCAC
GGCCATTTGTGACTCACCACAATGCGTTGAACATTGATCTCTACTTACGAATTGCGTTGGAATTACATTTGAAGCGCCTG
ATCGTTGGTGGCATGGAAAAGGTTTATGAGATTGGTCGGGTTTTCCGTAATGAAGGGATGGACCGTGAACATAATCCTGA
ATTTACCATGATGGAAACTTATGTCGCCTATTTCGACTTCCATGATGTAATGGCTGAGACGGAAGGTATTTTTAAAGCCG
CAGCGCAAGCCGTAACTGACGATGGCATTGTCACTTATCATGATCAAAAAGTTGATTTCAATCAACCATTTAAGCAAATT
CACATGGTCGATGCGATTAAAGAAAAGACTGGGATTGATTTCTGGCAACCAATGTCAATTGAAGATGCACAAAAATTGGC
GGATGAACATCACGTGAAGTATGAACCATACTGGAAAGTTGGTCATATCATTAATGCGTTCTTTGAAGAATTTGTTGAAG
ACACGTTGAATGAGCCAACCTTTGTTTATGGTCATCCAGTTGAAATTTCGCCATTAGCTAAGAAGAATGAAGAAGATCCA
CGGTTTACTGATCGTTTTGAACTCTTCATCTTAGGTAATGAATACGCCAATGCGTTCAGTGAATTGAATGATCCAATTGA
CCAACGGCAACGTTTCGAAGCTCAGGCTGCGGAACGGACTGCTGGTAACGACGAAGCTGAACATATTGATGAAGACTTTG
TCGAGGCGTTGGAATACGGGATGCCGCCTACTGGTGGGTTAGGTATCGGGATTGATCGGTTGGTTATGTTAATGACGGAT
GCGGATTCGATTCGTGATGTCTTACTTTTCCCAACGATGCGTCCAGAAGAAGATAAATAA

Upstream 100 bases:

>100_bases
ATTAACTTGCATTTTGGCGGCGTGATTGTCAAAATTAACATGTAGTGATTGTTTAGGTACGGAATAAATCTGTACGCTAA
CTAGAAATGGAGGAACACTA

Downstream 100 bases:

>100_bases
TTAGATTTAAAACGGCAGTTAGCTTAGGGTTTCCTAAGCTAACTGCCGTTTTTGTTGGGTCAGATTGAGTAACTTTAGTT
CAGAAAGCGATTTATTGAAA

Product: lysyl-tRNA synthetase

Products: NA

Alternate protein names: Lysine--tRNA ligase; LysRS

Number of amino acids: Translated: 499; Mature: 498

Protein sequence:

>499_residues
MARQEQTMNDQLKVRREKMDELREEGIDPFGHRFERTDLAQDLQDKYGDMDKDELDAKQVVATIAGRMLAKRGKGKVGFA
DIWDRSGKMQLYIRKDVVGEDTYHIFKRSDIGDFLGITGQVFKTDFGELTIKVTGLTFLSKALRPLPDKFHGLQNVEQIY
RQRYLDLISNRDSFDRFLKRTKIISAIRHHLDDQGFTEVETPMLHNQAGGAAARPFVTHHNALNIDLYLRIALELHLKRL
IVGGMEKVYEIGRVFRNEGMDREHNPEFTMMETYVAYFDFHDVMAETEGIFKAAAQAVTDDGIVTYHDQKVDFNQPFKQI
HMVDAIKEKTGIDFWQPMSIEDAQKLADEHHVKYEPYWKVGHIINAFFEEFVEDTLNEPTFVYGHPVEISPLAKKNEEDP
RFTDRFELFILGNEYANAFSELNDPIDQRQRFEAQAAERTAGNDEAEHIDEDFVEALEYGMPPTGGLGIGIDRLVMLMTD
ADSIRDVLLFPTMRPEEDK

Sequences:

>Translated_499_residues
MARQEQTMNDQLKVRREKMDELREEGIDPFGHRFERTDLAQDLQDKYGDMDKDELDAKQVVATIAGRMLAKRGKGKVGFA
DIWDRSGKMQLYIRKDVVGEDTYHIFKRSDIGDFLGITGQVFKTDFGELTIKVTGLTFLSKALRPLPDKFHGLQNVEQIY
RQRYLDLISNRDSFDRFLKRTKIISAIRHHLDDQGFTEVETPMLHNQAGGAAARPFVTHHNALNIDLYLRIALELHLKRL
IVGGMEKVYEIGRVFRNEGMDREHNPEFTMMETYVAYFDFHDVMAETEGIFKAAAQAVTDDGIVTYHDQKVDFNQPFKQI
HMVDAIKEKTGIDFWQPMSIEDAQKLADEHHVKYEPYWKVGHIINAFFEEFVEDTLNEPTFVYGHPVEISPLAKKNEEDP
RFTDRFELFILGNEYANAFSELNDPIDQRQRFEAQAAERTAGNDEAEHIDEDFVEALEYGMPPTGGLGIGIDRLVMLMTD
ADSIRDVLLFPTMRPEEDK
>Mature_498_residues
ARQEQTMNDQLKVRREKMDELREEGIDPFGHRFERTDLAQDLQDKYGDMDKDELDAKQVVATIAGRMLAKRGKGKVGFAD
IWDRSGKMQLYIRKDVVGEDTYHIFKRSDIGDFLGITGQVFKTDFGELTIKVTGLTFLSKALRPLPDKFHGLQNVEQIYR
QRYLDLISNRDSFDRFLKRTKIISAIRHHLDDQGFTEVETPMLHNQAGGAAARPFVTHHNALNIDLYLRIALELHLKRLI
VGGMEKVYEIGRVFRNEGMDREHNPEFTMMETYVAYFDFHDVMAETEGIFKAAAQAVTDDGIVTYHDQKVDFNQPFKQIH
MVDAIKEKTGIDFWQPMSIEDAQKLADEHHVKYEPYWKVGHIINAFFEEFVEDTLNEPTFVYGHPVEISPLAKKNEEDPR
FTDRFELFILGNEYANAFSELNDPIDQRQRFEAQAAERTAGNDEAEHIDEDFVEALEYGMPPTGGLGIGIDRLVMLMTDA
DSIRDVLLFPTMRPEEDK

Specific function: Unknown

COG id: COG1190

COG function: function code J; Lysyl-tRNA synthetase (class II)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI5031815, Length=507, Percent_Identity=42.0118343195266, Blast_Score=387, Evalue=1e-108,
Organism=Homo sapiens, GI194272210, Length=507, Percent_Identity=42.0118343195266, Blast_Score=387, Evalue=1e-107,
Organism=Homo sapiens, GI226491502, Length=453, Percent_Identity=23.1788079470199, Blast_Score=82, Evalue=1e-15,
Organism=Escherichia coli, GI1789256, Length=490, Percent_Identity=50.6122448979592, Blast_Score=491, Evalue=1e-140,
Organism=Escherichia coli, GI1790571, Length=491, Percent_Identity=49.4908350305499, Blast_Score=489, Evalue=1e-139,
Organism=Escherichia coli, GI87082379, Length=333, Percent_Identity=33.9339339339339, Blast_Score=162, Evalue=5e-41,
Organism=Escherichia coli, GI1788173, Length=240, Percent_Identity=26.25, Blast_Score=74, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI17535925, Length=503, Percent_Identity=42.1471172962227, Blast_Score=394, Evalue=1e-110,
Organism=Caenorhabditis elegans, GI17535927, Length=501, Percent_Identity=42.315369261477, Blast_Score=393, Evalue=1e-109,
Organism=Caenorhabditis elegans, GI71994340, Length=501, Percent_Identity=42.315369261477, Blast_Score=392, Evalue=1e-109,
Organism=Saccharomyces cerevisiae, GI6320242, Length=511, Percent_Identity=40.7045009784736, Blast_Score=375, Evalue=1e-105,
Organism=Saccharomyces cerevisiae, GI6324256, Length=485, Percent_Identity=33.1958762886598, Blast_Score=212, Evalue=1e-55,
Organism=Saccharomyces cerevisiae, GI6321807, Length=345, Percent_Identity=26.9565217391304, Blast_Score=90, Evalue=1e-18,
Organism=Drosophila melanogaster, GI24640849, Length=511, Percent_Identity=41.6829745596869, Blast_Score=399, Evalue=1e-111,
Organism=Drosophila melanogaster, GI24640851, Length=511, Percent_Identity=41.6829745596869, Blast_Score=398, Evalue=1e-111,
Organism=Drosophila melanogaster, GI17136276, Length=468, Percent_Identity=23.0769230769231, Blast_Score=87, Evalue=3e-17,

Paralogues:

None

Copy number: 1200 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYK_LACPL (Q88Z28)

Other databases:

- EMBL:   AL935253
- RefSeq:   NP_784326.1
- ProteinModelPortal:   Q88Z28
- SMR:   Q88Z28
- GeneID:   1061906
- GenomeReviews:   AL935263_GR
- KEGG:   lpl:lp_0550
- NMPDR:   fig|220668.1.peg.460
- HOGENOM:   HBG631383
- OMA:   RNEGMDH
- ProtClustDB:   PRK00484
- BioCyc:   LPLA220668:LP_0550-MONOMER
- BRENDA:   6.1.1.6
- GO:   GO:0005737
- HAMAP:   MF_00252
- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR002313
- InterPro:   IPR018149
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004365
- Gene3D:   G3DSA:2.40.50.140
- PANTHER:   PTHR22594
- PANTHER:   PTHR22594:SF4
- PRINTS:   PR00982
- TIGRFAMs:   TIGR00499

Pfam domain/function: PF00152 tRNA-synt_2; PF01336 tRNA_anti; SSF50249 Nucleic_acid_OB

EC number: =6.1.1.6

Molecular weight: Translated: 57630; Mature: 57499

Theoretical pI: Translated: 4.82; Mature: 4.82

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARQEQTMNDQLKVRREKMDELREEGIDPFGHRFERTDLAQDLQDKYGDMDKDELDAKQV
CCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
VATIAGRMLAKRGKGKVGFADIWDRSGKMQLYIRKDVVGEDTYHIFKRSDIGDFLGITGQ
HHHHHHHHHHHCCCCCCCHHHHHCCCCCEEEEEECCCCCCCHHHHHCCCCCHHHHCCCCH
VFKTDFGELTIKVTGLTFLSKALRPLPDKFHGLQNVEQIYRQRYLDLISNRDSFDRFLKR
HHHCCCCEEEEEEEHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
TKIISAIRHHLDDQGFTEVETPMLHNQAGGAAARPFVTHHNALNIDLYLRIALELHLKRL
HHHHHHHHHHCCCCCCCHHCCCHHHCCCCCCCCCCEEECCCEEEEEHHHHHHHHHHHHHH
IVGGMEKVYEIGRVFRNEGMDREHNPEFTMMETYVAYFDFHDVMAETEGIFKAAAQAVTD
HHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
DGIVTYHDQKVDFNQPFKQIHMVDAIKEKTGIDFWQPMSIEDAQKLADEHHVKYEPYWKV
CCEEEEECCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCCCCCHHH
GHIINAFFEEFVEDTLNEPTFVYGHPVEISPLAKKNEEDPRFTDRFELFILGNEYANAFS
HHHHHHHHHHHHHHHCCCCEEEECCCCEECCCCCCCCCCCCCCCEEEEEEECCHHHHHHH
ELNDPIDQRQRFEAQAAERTAGNDEAEHIDEDFVEALEYGMPPTGGLGIGIDRLVMLMTD
HHCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHCC
ADSIRDVLLFPTMRPEEDK
HHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure 
ARQEQTMNDQLKVRREKMDELREEGIDPFGHRFERTDLAQDLQDKYGDMDKDELDAKQV
CCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
VATIAGRMLAKRGKGKVGFADIWDRSGKMQLYIRKDVVGEDTYHIFKRSDIGDFLGITGQ
HHHHHHHHHHHCCCCCCCHHHHHCCCCCEEEEEECCCCCCCHHHHHCCCCCHHHHCCCCH
VFKTDFGELTIKVTGLTFLSKALRPLPDKFHGLQNVEQIYRQRYLDLISNRDSFDRFLKR
HHHCCCCEEEEEEEHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
TKIISAIRHHLDDQGFTEVETPMLHNQAGGAAARPFVTHHNALNIDLYLRIALELHLKRL
HHHHHHHHHHCCCCCCCHHCCCHHHCCCCCCCCCCEEECCCEEEEEHHHHHHHHHHHHHH
IVGGMEKVYEIGRVFRNEGMDREHNPEFTMMETYVAYFDFHDVMAETEGIFKAAAQAVTD
HHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
DGIVTYHDQKVDFNQPFKQIHMVDAIKEKTGIDFWQPMSIEDAQKLADEHHVKYEPYWKV
CCEEEEECCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCCCCCHHH
GHIINAFFEEFVEDTLNEPTFVYGHPVEISPLAKKNEEDPRFTDRFELFILGNEYANAFS
HHHHHHHHHHHHHHHCCCCEEEECCCCEECCCCCCCCCCCCCCCEEEEEEECCHHHHHHH
ELNDPIDQRQRFEAQAAERTAGNDEAEHIDEDFVEALEYGMPPTGGLGIGIDRLVMLMTD
HHCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHCC
ADSIRDVLLFPTMRPEEDK
HHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12566566