Click here to switch to the map view.

The map label for this gene is dus1 [H]

Identifier: 28377433

GI number: 28377433

Start: 499655

End: 500662

Strand: Direct

Name: dus1 [H]

Synonym: lp_0549

Alternate gene names: 28377433

Gene position: 499655-500662 (Clockwise)

Preceding gene: 28377432

Following gene: 28377434

Centisome position: 15.1

GC content: 50.3

Gene sequence:

>1008_bases
ATGAATCAAGAGTGGCAGATCGGTGATGTGACGATTCCCAATCGTGTCGTAGTGGCACCGATGGCCGGCGTGACTAACGC
GGCCTTTCGAGTAATCTGCAAGGATTTTGGTGCGGGGTTAGTCGTTTGTGAGATGATTTCTGATCGCGGGATCATGTATC
ATAATCGTAAGACCCTGGAAATGATGTTTGTTGATCCAAAAGAACATCCAATGAGTATTCAAATCTTTGGTGGTTCGAAA
GCAACACTGGTTCAAGCCGCCCAGTTTGTCGATCAGCAGACCAATGCTGATATTATTGATATTAATATGGGCTGTCCGGT
GAATAAAGTGGTTAAGACTGATGCGGGCGCTAAGTGGCTTTTAAGTCCAGATAAAGTCTACGAGATGGTCGCGGCTGTGA
CTGCTGCGGTTAATAAACCAGTGACGGTCAAGATGCGAACTGGTTGGGACGCAGAGCACGTATATGCCGTCGAGAATGCG
CGGGCTGCCGAACGAGCTGGTGCAGCTGCCGTTGCAATGCATGGACGGACTCGTAAGCAGATGTACCAAGGCCATGCTGA
TTGGGACGTGTTAAAACGGGTGGCGGATGCCCTCACGATTCCATTGATGGGTAATGGTGACGTTCAGACACCTCAAGATG
CTGCGCGGATGCTTGATGAAGTCGGGGCCGATGCCGTCATGATTGGCCGGGCGGTAGAAGGTAATCCGTGGATGCTTACC
CAGACAGTACACTATTTAGCAACCGGGGAGTTACTAACGCCACCCACACCGATGGCTAAGATGCAAACGGCAATGGAACA
TTTGCAGCGGTTAGTGGTTTTAAAAGGTGAGCGGGGCGGATGTCATGAATTCCGTGAACAAGCGCCGTACTATCTAAAAG
GCATCCCAAGGTCGGCACGGACCAAAGTGGCTTTAATGGAGGCAAGCTCGTTAGCAGAAATGATGCGCATTTTAACGCAG
TTTCAGGAGCAGACGGCAACTTATTTGGCTAGTCACGCGGTAAAGTGA

Upstream 100 bases:

>100_bases
ATCATGGTGCCACGGCAGTTTGTCATTTTTGTGGGGACCAGTATCAGTTCAGTGAACGCGAACTAGAAGCAGTGCTCTCA
CGGTCCAAGGGTGACGCTTA

Downstream 100 bases:

>100_bases
ATAATTAACTTGCATTTTGGCGGCGTGATTGTCAAAATTAACATGTAGTGATTGTTTAGGTACGGAATAAATCTGTACGC
TAACTAGAAATGGAGGAACA

Product: transcription regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 335; Mature: 335

Protein sequence:

>335_residues
MNQEWQIGDVTIPNRVVVAPMAGVTNAAFRVICKDFGAGLVVCEMISDRGIMYHNRKTLEMMFVDPKEHPMSIQIFGGSK
ATLVQAAQFVDQQTNADIIDINMGCPVNKVVKTDAGAKWLLSPDKVYEMVAAVTAAVNKPVTVKMRTGWDAEHVYAVENA
RAAERAGAAAVAMHGRTRKQMYQGHADWDVLKRVADALTIPLMGNGDVQTPQDAARMLDEVGADAVMIGRAVEGNPWMLT
QTVHYLATGELLTPPTPMAKMQTAMEHLQRLVVLKGERGGCHEFREQAPYYLKGIPRSARTKVALMEASSLAEMMRILTQ
FQEQTATYLASHAVK

Sequences:

>Translated_335_residues
MNQEWQIGDVTIPNRVVVAPMAGVTNAAFRVICKDFGAGLVVCEMISDRGIMYHNRKTLEMMFVDPKEHPMSIQIFGGSK
ATLVQAAQFVDQQTNADIIDINMGCPVNKVVKTDAGAKWLLSPDKVYEMVAAVTAAVNKPVTVKMRTGWDAEHVYAVENA
RAAERAGAAAVAMHGRTRKQMYQGHADWDVLKRVADALTIPLMGNGDVQTPQDAARMLDEVGADAVMIGRAVEGNPWMLT
QTVHYLATGELLTPPTPMAKMQTAMEHLQRLVVLKGERGGCHEFREQAPYYLKGIPRSARTKVALMEASSLAEMMRILTQ
FQEQTATYLASHAVK
>Mature_335_residues
MNQEWQIGDVTIPNRVVVAPMAGVTNAAFRVICKDFGAGLVVCEMISDRGIMYHNRKTLEMMFVDPKEHPMSIQIFGGSK
ATLVQAAQFVDQQTNADIIDINMGCPVNKVVKTDAGAKWLLSPDKVYEMVAAVTAAVNKPVTVKMRTGWDAEHVYAVENA
RAAERAGAAAVAMHGRTRKQMYQGHADWDVLKRVADALTIPLMGNGDVQTPQDAARMLDEVGADAVMIGRAVEGNPWMLT
QTVHYLATGELLTPPTPMAKMQTAMEHLQRLVVLKGERGGCHEFREQAPYYLKGIPRSARTKVALMEASSLAEMMRILTQ
FQEQTATYLASHAVK

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs [H]

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family [H]

Homologues:

Organism=Homo sapiens, GI239788483, Length=234, Percent_Identity=29.0598290598291, Blast_Score=123, Evalue=2e-28,
Organism=Homo sapiens, GI239788462, Length=233, Percent_Identity=29.1845493562232, Blast_Score=121, Evalue=1e-27,
Organism=Homo sapiens, GI8923374, Length=245, Percent_Identity=30.2040816326531, Blast_Score=107, Evalue=1e-23,
Organism=Homo sapiens, GI40807366, Length=225, Percent_Identity=30.2222222222222, Blast_Score=99, Evalue=8e-21,
Organism=Homo sapiens, GI31742496, Length=226, Percent_Identity=27.4336283185841, Blast_Score=90, Evalue=3e-18,
Organism=Escherichia coli, GI1789660, Length=290, Percent_Identity=40, Blast_Score=215, Evalue=3e-57,
Organism=Escherichia coli, GI1788462, Length=242, Percent_Identity=30.9917355371901, Blast_Score=98, Evalue=8e-22,
Organism=Caenorhabditis elegans, GI17543114, Length=237, Percent_Identity=29.957805907173, Blast_Score=119, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI17507177, Length=231, Percent_Identity=31.1688311688312, Blast_Score=100, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI25144369, Length=227, Percent_Identity=25.1101321585903, Blast_Score=84, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17510279, Length=208, Percent_Identity=26.9230769230769, Blast_Score=75, Evalue=5e-14,
Organism=Saccharomyces cerevisiae, GI6323560, Length=233, Percent_Identity=29.1845493562232, Blast_Score=97, Evalue=3e-21,
Organism=Saccharomyces cerevisiae, GI6323433, Length=259, Percent_Identity=27.027027027027, Blast_Score=87, Evalue=3e-18,
Organism=Saccharomyces cerevisiae, GI6323437, Length=226, Percent_Identity=23.4513274336283, Blast_Score=83, Evalue=7e-17,
Organism=Drosophila melanogaster, GI24585320, Length=236, Percent_Identity=31.7796610169492, Blast_Score=125, Evalue=6e-29,
Organism=Drosophila melanogaster, GI24580595, Length=261, Percent_Identity=30.2681992337165, Blast_Score=108, Evalue=4e-24,
Organism=Drosophila melanogaster, GI19920448, Length=261, Percent_Identity=30.2681992337165, Blast_Score=108, Evalue=4e-24,
Organism=Drosophila melanogaster, GI45549423, Length=239, Percent_Identity=29.7071129707113, Blast_Score=98, Evalue=7e-21,
Organism=Drosophila melanogaster, GI19921524, Length=228, Percent_Identity=28.0701754385965, Blast_Score=84, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR004652
- InterPro:   IPR001269
- InterPro:   IPR018517 [H]

Pfam domain/function: PF01207 Dus [H]

EC number: NA

Molecular weight: Translated: 36799; Mature: 36799

Theoretical pI: Translated: 7.64; Mature: 7.64

Prosite motif: PS01136 UPF0034

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
6.6 %Met     (Translated Protein)
7.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
6.6 %Met     (Mature Protein)
7.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNQEWQIGDVTIPNRVVVAPMAGVTNAAFRVICKDFGAGLVVCEMISDRGIMYHNRKTLE
CCCCCCCCCEECCCEEEEECCCCCHHHHHHHHHHHHCCHHEEEEHHHCCCEEEECCCEEE
MMFVDPKEHPMSIQIFGGSKATLVQAAQFVDQQTNADIIDINMGCPVNKVVKTDAGAKWL
EEEECCCCCCEEEEEECCCHHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHCCCCCCEEE
LSPDKVYEMVAAVTAAVNKPVTVKMRTGWDAEHVYAVENARAAERAGAAAVAMHGRTRKQ
CCHHHHHHHHHHHHHHCCCCEEEEEECCCCCCEEEEECCCHHHHHCCCEEEECCCCHHHH
MYQGHADWDVLKRVADALTIPLMGNGDVQTPQDAARMLDEVGADAVMIGRAVEGNPWMLT
HHCCCCCHHHHHHHHHHEEEEEECCCCCCCHHHHHHHHHHHCCCEEEEEEEECCCCEEHH
QTVHYLATGELLTPPTPMAKMQTAMEHLQRLVVLKGERGGCHEFREQAPYYLKGIPRSAR
HHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHEEEECCCCCHHHHHHCCCCEEECCCCCHH
TKVALMEASSLAEMMRILTQFQEQTATYLASHAVK
HEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNQEWQIGDVTIPNRVVVAPMAGVTNAAFRVICKDFGAGLVVCEMISDRGIMYHNRKTLE
CCCCCCCCCEECCCEEEEECCCCCHHHHHHHHHHHHCCHHEEEEHHHCCCEEEECCCEEE
MMFVDPKEHPMSIQIFGGSKATLVQAAQFVDQQTNADIIDINMGCPVNKVVKTDAGAKWL
EEEECCCCCCEEEEEECCCHHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHCCCCCCEEE
LSPDKVYEMVAAVTAAVNKPVTVKMRTGWDAEHVYAVENARAAERAGAAAVAMHGRTRKQ
CCHHHHHHHHHHHHHHCCCCEEEEEECCCCCCEEEEECCCHHHHHCCCEEEECCCCHHHH
MYQGHADWDVLKRVADALTIPLMGNGDVQTPQDAARMLDEVGADAVMIGRAVEGNPWMLT
HHCCCCCHHHHHHHHHHEEEEEECCCCCCCHHHHHHHHHHHCCCEEEEEEEECCCCEEHH
QTVHYLATGELLTPPTPMAKMQTAMEHLQRLVVLKGERGGCHEFREQAPYYLKGIPRSAR
HHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHEEEECCCCCHHHHHHCCCCEEECCCCCHH
TKVALMEASSLAEMMRILTQFQEQTATYLASHAVK
HEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7584024; 9384377 [H]