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The map label for this gene is murB

Identifier: 62185354

GI number: 62185354

Start: 854027

End: 854917

Strand: Direct

Name: murB

Synonym: CAB741

Alternate gene names: 62185354

Gene position: 854027-854917 (Clockwise)

Preceding gene: 62185353

Following gene: 62185358

Centisome position: 74.63

GC content: 39.62

Gene sequence:

>891_bases
GTGAAAGCCTCTACTGTCATTCGTTTCCCTTTTTCAGTTCGCCGTAGTGTTTGGTTGAATAAGTATTCTACATTTCGTAT
CGGAGGGCCTGCGAATTACTTTAAAGTAGTGCATTCAGCTAGCGAAGCACAGCAGGTAATTCAGTTCTTACATAGTCATA
ACTATCCATTTATTATCGTCGGGAAAGGTTCGAATTGCTTATTCGATGATCGAGGGTTTGATGGTTTTGTTTTGTGTAAT
GGCATACAAGGAAAAGAATTTGTCTCTGAAACGACCATCAAAGTGTATTCGGGAACTTCTTTTTCTTTTTTAGGGAAAAC
TCTTTCTTCTTCAGGATATTCAGGTTTAGAATTTGCCGTAGGCATTCCAGGATCGGTGGGTGGGGCTGTATTTATGAATG
CTGGCATTGGCAATCAGGATACGGCTGCTGTAATTGAAAGCGTGGAAGTCATTAATTCTAAAGGAGAAATCCTTTCATAT
AATACTGAAGAATTAGGATTTGGTTATCGGACATCGCGTTTTCAACATTGCAATGAATTTATTCTTTCTGCAACTTTCCG
CCTATCTAGGAATTCTTCATCCGTAAAAATTGCAAAAGACTTGCTCCGGAATAGATTACTTTCTCAACCTTACCAACAAC
CTTCTACAGGGTGTATTTTCCGTAATCCCCCAGGAAATTCCGCGGGGAAACTCATCGATGAAGCTGGTTTAAAAGGCTTT
TCTCTAGGAGGAGCTCAAATATCTCCAAAGCATGCGAACTTCATTGTGAATACGGGCAGAGCGACATCTCAAGAAGTTAA
ACAGCTTATTCAAATAGTTCAAGACAAGTTAAAATCACAGGGAATAAACTTAGAAGAAGAAGTGCGCATTATTCCTTACC
AACTCCCCTAA

Upstream 100 bases:

>100_bases
AATGATATTTTTCGTTTAGCAGCGCCGATGACACACCCTGATACTTCTCTCATGTGTTGTTAGACTTAGCGTTATTTTAA
ACTTATCTGGAATTCTAAAT

Downstream 100 bases:

>100_bases
GAGAACGGAACACAAAAAGCCTAGGGGCCTTATATCTATTCATAATATAGAAAGAGCCCACTTCCCAAAGCTTAGGATCT
AATGTACATGCCAGTCCTTC

Product: UDP-N-acetylenolpyruvoylglucosamine reductase

Products: NA

Alternate protein names: UDP-N-acetylmuramate dehydrogenase

Number of amino acids: Translated: 296; Mature: 296

Protein sequence:

>296_residues
MKASTVIRFPFSVRRSVWLNKYSTFRIGGPANYFKVVHSASEAQQVIQFLHSHNYPFIIVGKGSNCLFDDRGFDGFVLCN
GIQGKEFVSETTIKVYSGTSFSFLGKTLSSSGYSGLEFAVGIPGSVGGAVFMNAGIGNQDTAAVIESVEVINSKGEILSY
NTEELGFGYRTSRFQHCNEFILSATFRLSRNSSSVKIAKDLLRNRLLSQPYQQPSTGCIFRNPPGNSAGKLIDEAGLKGF
SLGGAQISPKHANFIVNTGRATSQEVKQLIQIVQDKLKSQGINLEEEVRIIPYQLP

Sequences:

>Translated_296_residues
MKASTVIRFPFSVRRSVWLNKYSTFRIGGPANYFKVVHSASEAQQVIQFLHSHNYPFIIVGKGSNCLFDDRGFDGFVLCN
GIQGKEFVSETTIKVYSGTSFSFLGKTLSSSGYSGLEFAVGIPGSVGGAVFMNAGIGNQDTAAVIESVEVINSKGEILSY
NTEELGFGYRTSRFQHCNEFILSATFRLSRNSSSVKIAKDLLRNRLLSQPYQQPSTGCIFRNPPGNSAGKLIDEAGLKGF
SLGGAQISPKHANFIVNTGRATSQEVKQLIQIVQDKLKSQGINLEEEVRIIPYQLP
>Mature_296_residues
MKASTVIRFPFSVRRSVWLNKYSTFRIGGPANYFKVVHSASEAQQVIQFLHSHNYPFIIVGKGSNCLFDDRGFDGFVLCN
GIQGKEFVSETTIKVYSGTSFSFLGKTLSSSGYSGLEFAVGIPGSVGGAVFMNAGIGNQDTAAVIESVEVINSKGEILSY
NTEELGFGYRTSRFQHCNEFILSATFRLSRNSSSVKIAKDLLRNRLLSQPYQQPSTGCIFRNPPGNSAGKLIDEAGLKGF
SLGGAQISPKHANFIVNTGRATSQEVKQLIQIVQDKLKSQGINLEEEVRIIPYQLP

Specific function: Cell wall formation

COG id: COG0812

COG function: function code M; UDP-N-acetylmuramate dehydrogenase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FAD-binding PCMH-type domain

Homologues:

Organism=Escherichia coli, GI1790407, Length=328, Percent_Identity=27.4390243902439, Blast_Score=95, Evalue=5e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MURB_CHLAB (Q5L5A3)

Other databases:

- EMBL:   CR848038
- RefSeq:   YP_220139.1
- ProteinModelPortal:   Q5L5A3
- SMR:   Q5L5A3
- GeneID:   3337855
- GenomeReviews:   CR848038_GR
- KEGG:   cab:CAB741
- HOGENOM:   HBG686573
- OMA:   LESSKIP
- PhylomeDB:   Q5L5A3
- ProtClustDB:   PRK13905
- BioCyc:   CABO218497:CAB741-MONOMER
- BRENDA:   1.1.1.158
- GO:   GO:0005737
- HAMAP:   MF_00037
- InterPro:   IPR016169
- InterPro:   IPR016166
- InterPro:   IPR016167
- InterPro:   IPR003170
- InterPro:   IPR011601
- InterPro:   IPR006094
- Gene3D:   G3DSA:3.30.465.10
- Gene3D:   G3DSA:3.30.43.10
- Gene3D:   G3DSA:3.90.78.10
- PANTHER:   PTHR21071
- TIGRFAMs:   TIGR00179

Pfam domain/function: PF01565 FAD_binding_4; PF02873 MurB_C; SSF56176 FAD-binding_2; SSF56194 MurB_C

EC number: =1.1.1.158

Molecular weight: Translated: 32373; Mature: 32373

Theoretical pI: Translated: 9.64; Mature: 9.64

Prosite motif: PS51387 FAD_PCMH

Important sites: ACT_SITE 170-170 ACT_SITE 218-218 ACT_SITE 287-287

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKASTVIRFPFSVRRSVWLNKYSTFRIGGPANYFKVVHSASEAQQVIQFLHSHNYPFIIV
CCCCEEEECCHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEE
GKGSNCLFDDRGFDGFVLCNGIQGKEFVSETTIKVYSGTSFSFLGKTLSSSGYSGLEFAV
ECCCCEEEECCCCCCEEEECCCCCHHHHHHCEEEEEECCCHHHHHHHHHCCCCCCCEEEE
GIPGSVGGAVFMNAGIGNQDTAAVIESVEVINSKGEILSYNTEELGFGYRTSRFQHCNEF
ECCCCCCCEEEEECCCCCCHHHHHHHHHHHHCCCCCEEEECHHHHCCCCHHHHHHHHHHH
ILSATFRLSRNSSSVKIAKDLLRNRLLSQPYQQPSTGCIFRNPPGNSAGKLIDEAGLKGF
HHHHHEEECCCCCCHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCHHHHHHHHCCCEE
SLGGAQISPKHANFIVNTGRATSQEVKQLIQIVQDKLKSQGINLEEEVRIIPYQLP
ECCCCEECCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECC
>Mature Secondary Structure
MKASTVIRFPFSVRRSVWLNKYSTFRIGGPANYFKVVHSASEAQQVIQFLHSHNYPFIIV
CCCCEEEECCHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEE
GKGSNCLFDDRGFDGFVLCNGIQGKEFVSETTIKVYSGTSFSFLGKTLSSSGYSGLEFAV
ECCCCEEEECCCCCCEEEECCCCCHHHHHHCEEEEEECCCHHHHHHHHHCCCCCCCEEEE
GIPGSVGGAVFMNAGIGNQDTAAVIESVEVINSKGEILSYNTEELGFGYRTSRFQHCNEF
ECCCCCCCEEEEECCCCCCHHHHHHHHHHHHCCCCCEEEECHHHHCCCCHHHHHHHHHHH
ILSATFRLSRNSSSVKIAKDLLRNRLLSQPYQQPSTGCIFRNPPGNSAGKLIDEAGLKGF
HHHHHEEECCCCCCHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCHHHHHHHHCCCEE
SLGGAQISPKHANFIVNTGRATSQEVKQLIQIVQDKLKSQGINLEEEVRIIPYQLP
ECCCCEECCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA