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The map label for this gene is oppA [H]

Identifier: 62185188

GI number: 62185188

Start: 647973

End: 649313

Strand: Direct

Name: oppA [H]

Synonym: CAB572

Alternate gene names: 62185188

Gene position: 647973-649313 (Clockwise)

Preceding gene: 62185187

Following gene: 62185189

Centisome position: 56.62

GC content: 39.52

Gene sequence:

>1341_bases
ATGACATTGAAGGTATTTGCTTTTTTTATGAAATACACATGGTGGTTGATTGCTGTTGTACTCACGTCTTCAATTTTAGG
AGGCTGTTTTTCAACAGCCTTAAGATCGTCAAAAACCCTTACGATTGCCATCCATGACGACCCGGAATCTTTATCCCCTG
AACAAGCGAAACGTGCTTTAGATCTTTCTGTATCTAAACTTATTTTCGAAGGACTTACAAGAGAAAATCCGAACAAACAT
AATCAAGTAGAATTTGCCCTAGCAAGTCACTATACCGTATCGGCAGATGAAAAAACCTATACGTTCTTTATCAAACATGG
GGCCCTATGGAGTAACGGCACTCCAATCACATCTCAGGATATTACTCGGGCATGGCAACATGCAAAAACTTGCTCTCCTC
ACCACCAAGCATTTGAAGGTATCCAGTTTAAAGCCTGTTCGCCATCCAGCATTACCTTAACACTCGATTCCCCAAATTCT
AATCTACTGCAATTGCTCGCTTTTCCAGCATTTTCTGTTTTCAACCCCGAAAATTTGAATATATTTAGTGGGCCTTTCCA
GTTAATCACTTACAATCCTGGGCATTGCCTCTTATTAGAAAAAAATCCTTACTACTATGATAAAGACAAGGTCCTGATCT
CCTGTGTTAACTTATTAGTTATTCCGGATTTGTATACCGGAGCTCTTTTACAAAATCGTGGAAAAATTCATTGGTTAGGG
CAACCCTGGCATCAAGGGTTGACAAAAGAACTGAAAGAAAATACTCCCTACCACTACACTTGCTACCCCGTAGAAGGAGC
TTTTTGGCTTGTACTGAATACAAAAGATCCAGTTTTATCTCATATCCATAACCGCTACAGATTAGCCGCCGCGATTAATA
GAGAAGAAATTATAGATTATGCTCTACAAGGCAACCAAGATCCCGCCTATACACTATCTAGGCAATCCTCTCCGCAGCAC
CCACAGAAAAAACAAAAACTGATTACCCCCACCGAAAAACTGACTCTAACCTATCCTTCAAATATTTTACGATGCCAACG
CATCGCAGAAATTCTTAAAGAACAATGCAAATCCGTAGGGATTGAGTTATTTCTTGAAGGATTGGAATACCATGTTTTCT
TAACCAAAAGACAAATGTGTGATTTTACTATAGCTACGGCAACAGGAGTCGCTTATTACCCAAGCGCTTCGCTAATCCCA
CAAGCAGACAGGTTAATTAAAAACTTAGAGATTATTCCTATCTACCATATGAGCTACGATTATATCACTCTATCTCCAAT
AGAGAAAATTATTCATAATGCCTCTGGAGCCGTCGATCTTAAATACGCACGCTTACCTTAA

Upstream 100 bases:

>100_bases
GCTATCTTGAAAGTTCCGCAATCCACTTATCCTTCTAAAAGATTTGCAGTACAATCAGTACGGCACAGTGGGATAAGTCA
CAGTGTCTTTCCGTTAAAAA

Downstream 100 bases:

>100_bases
AAACTCGCCAAAAATACAACAGAAAATACAATTTTTTTCACAAATTCAAAAATTGGAAATTATTTTTTTAATCAGTAATC
TAACTCCAACTGACTATTTA

Product: putative ABC transporter peptide periplasmic binding lipoprotein

Products: ADP; phosphate; oligopeptides [Cytoplasm] [C]

Alternate protein names: Stage 0 sporulation protein KA [H]

Number of amino acids: Translated: 446; Mature: 445

Protein sequence:

>446_residues
MTLKVFAFFMKYTWWLIAVVLTSSILGGCFSTALRSSKTLTIAIHDDPESLSPEQAKRALDLSVSKLIFEGLTRENPNKH
NQVEFALASHYTVSADEKTYTFFIKHGALWSNGTPITSQDITRAWQHAKTCSPHHQAFEGIQFKACSPSSITLTLDSPNS
NLLQLLAFPAFSVFNPENLNIFSGPFQLITYNPGHCLLLEKNPYYYDKDKVLISCVNLLVIPDLYTGALLQNRGKIHWLG
QPWHQGLTKELKENTPYHYTCYPVEGAFWLVLNTKDPVLSHIHNRYRLAAAINREEIIDYALQGNQDPAYTLSRQSSPQH
PQKKQKLITPTEKLTLTYPSNILRCQRIAEILKEQCKSVGIELFLEGLEYHVFLTKRQMCDFTIATATGVAYYPSASLIP
QADRLIKNLEIIPIYHMSYDYITLSPIEKIIHNASGAVDLKYARLP

Sequences:

>Translated_446_residues
MTLKVFAFFMKYTWWLIAVVLTSSILGGCFSTALRSSKTLTIAIHDDPESLSPEQAKRALDLSVSKLIFEGLTRENPNKH
NQVEFALASHYTVSADEKTYTFFIKHGALWSNGTPITSQDITRAWQHAKTCSPHHQAFEGIQFKACSPSSITLTLDSPNS
NLLQLLAFPAFSVFNPENLNIFSGPFQLITYNPGHCLLLEKNPYYYDKDKVLISCVNLLVIPDLYTGALLQNRGKIHWLG
QPWHQGLTKELKENTPYHYTCYPVEGAFWLVLNTKDPVLSHIHNRYRLAAAINREEIIDYALQGNQDPAYTLSRQSSPQH
PQKKQKLITPTEKLTLTYPSNILRCQRIAEILKEQCKSVGIELFLEGLEYHVFLTKRQMCDFTIATATGVAYYPSASLIP
QADRLIKNLEIIPIYHMSYDYITLSPIEKIIHNASGAVDLKYARLP
>Mature_445_residues
TLKVFAFFMKYTWWLIAVVLTSSILGGCFSTALRSSKTLTIAIHDDPESLSPEQAKRALDLSVSKLIFEGLTRENPNKHN
QVEFALASHYTVSADEKTYTFFIKHGALWSNGTPITSQDITRAWQHAKTCSPHHQAFEGIQFKACSPSSITLTLDSPNSN
LLQLLAFPAFSVFNPENLNIFSGPFQLITYNPGHCLLLEKNPYYYDKDKVLISCVNLLVIPDLYTGALLQNRGKIHWLGQ
PWHQGLTKELKENTPYHYTCYPVEGAFWLVLNTKDPVLSHIHNRYRLAAAINREEIIDYALQGNQDPAYTLSRQSSPQHP
QKKQKLITPTEKLTLTYPSNILRCQRIAEILKEQCKSVGIELFLEGLEYHVFLTKRQMCDFTIATATGVAYYPSASLIPQ
ADRLIKNLEIIPIYHMSYDYITLSPIEKIIHNASGAVDLKYARLP

Specific function: This protein is a component of the oligopeptide permease, a binding protein-dependent transport system, It binds peptides up to five amino acids long with high affinity. Also required for sporulation and competence [H]

COG id: COG0747

COG function: function code E; ABC-type dipeptide transport system, periplasmic component

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1787495, Length=442, Percent_Identity=22.3981900452489, Blast_Score=86, Evalue=6e-18,
Organism=Escherichia coli, GI87081878, Length=422, Percent_Identity=23.2227488151659, Blast_Score=85, Evalue=9e-18,
Organism=Escherichia coli, GI1789397, Length=445, Percent_Identity=21.5730337078652, Blast_Score=83, Evalue=4e-17,
Organism=Escherichia coli, GI1787052, Length=383, Percent_Identity=21.9321148825065, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1787762, Length=404, Percent_Identity=22.2772277227723, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1789887, Length=366, Percent_Identity=26.775956284153, Blast_Score=67, Evalue=2e-12,

Paralogues:

None

Copy number: 760 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 500 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2360 Molecules/Cell In: Stationary-Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 50585; Mature: 50453

Theoretical pI: Translated: 8.15; Mature: 8.15

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLKVFAFFMKYTWWLIAVVLTSSILGGCFSTALRSSKTLTIAIHDDPESLSPEQAKRAL
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHH
DLSVSKLIFEGLTRENPNKHNQVEFALASHYTVSADEKTYTFFIKHGALWSNGTPITSQD
HHHHHHHHHHHHCCCCCCCCCEEEEEEEECEEEECCCCEEEEEEEECCEECCCCCCCHHH
ITRAWQHAKTCSPHHQAFEGIQFKACSPSSITLTLDSPNSNLLQLLAFPAFSVFNPENLN
HHHHHHHHCCCCHHHHHHCCCEEEECCCCEEEEEEECCCCHHHHHHHHCCCCCCCCCCCE
IFSGPFQLITYNPGHCLLLEKNPYYYDKDKVLISCVNLLVIPDLYTGALLQNRGKIHWLG
EECCCEEEEEECCCEEEEEECCCCEECCCHHHHHHHHHHHCCCHHHHHHHHCCCCEEECC
QPWHQGLTKELKENTPYHYTCYPVEGAFWLVLNTKDPVLSHIHNRYRLAAAINREEIIDY
CHHHHHHHHHHHHCCCCEEEEEEECCEEEEEEECCCHHHHHHHHHHHEEHHCCHHHHHHH
ALQGNQDPAYTLSRQSSPQHPQKKQKLITPTEKLTLTYPSNILRCQRIAEILKEQCKSVG
HHCCCCCCCEEECCCCCCCCCHHHHHCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHCC
IELFLEGLEYHVFLTKRQMCDFTIATATGVAYYPSASLIPQADRLIKNLEIIPIYHMSYD
HHHEEECCEEEEEEEECCCCCEEEEEECCEEECCCCCCCCHHHHHHHCCEEEEEEEEEEC
YITLSPIEKIIHNASGAVDLKYARLP
EEEHHHHHHHHHCCCCCEEEEEECCC
>Mature Secondary Structure 
TLKVFAFFMKYTWWLIAVVLTSSILGGCFSTALRSSKTLTIAIHDDPESLSPEQAKRAL
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHH
DLSVSKLIFEGLTRENPNKHNQVEFALASHYTVSADEKTYTFFIKHGALWSNGTPITSQD
HHHHHHHHHHHHCCCCCCCCCEEEEEEEECEEEECCCCEEEEEEEECCEECCCCCCCHHH
ITRAWQHAKTCSPHHQAFEGIQFKACSPSSITLTLDSPNSNLLQLLAFPAFSVFNPENLN
HHHHHHHHCCCCHHHHHHCCCEEEECCCCEEEEEEECCCCHHHHHHHHCCCCCCCCCCCE
IFSGPFQLITYNPGHCLLLEKNPYYYDKDKVLISCVNLLVIPDLYTGALLQNRGKIHWLG
EECCCEEEEEECCCEEEEEECCCCEECCCHHHHHHHHHHHCCCHHHHHHHHCCCCEEECC
QPWHQGLTKELKENTPYHYTCYPVEGAFWLVLNTKDPVLSHIHNRYRLAAAINREEIIDY
CHHHHHHHHHHHHCCCCEEEEEEECCEEEEEEECCCHHHHHHHHHHHEEHHCCHHHHHHH
ALQGNQDPAYTLSRQSSPQHPQKKQKLITPTEKLTLTYPSNILRCQRIAEILKEQCKSVG
HHCCCCCCCEEECCCCCCCCCHHHHHCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHCC
IELFLEGLEYHVFLTKRQMCDFTIATATGVAYYPSASLIPQADRLIKNLEIIPIYHMSYD
HHHEEECCEEEEEEEECCCCCEEEEEECCEEECCCCCCCCHHHHHHHCCEEEEEEEEEEC
YITLSPIEKIIHNASGAVDLKYARLP
EEEHHHHHHHHHCCCCCEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; oligopeptides [Periplasm]; H2O [C]

Specific reaction: ATP + oligopeptides [Periplasm] + H2O = ADP + phosphate + oligopeptides [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1901616; 1899858; 9384377 [H]