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The map label for this gene is purA

Identifier: 23100908

GI number: 23100908

Start: 3590401

End: 3591690

Strand: Reverse

Name: purA

Synonym: OB3453

Alternate gene names: 23100908

Gene position: 3591690-3590401 (Counterclockwise)

Preceding gene: 23100909

Following gene: 23100907

Centisome position: 98.93

GC content: 38.76

Gene sequence:

>1290_bases
ATGTCCTCAGTAGTTGTTGTAGGTACCCAATGGGGAGATGAAGGGAAAGGGAAGATTACAGATTTTCTTTCTCAAAATGC
AGAAGTAGTCGCAAGATATCAAGGTGGTAATAATGCTGGACATACAATCAAATTTGATGATGTGACATATAAATTACATT
TGATTCCATCTGGTATTTTCTTTGAGGATAAGATTTGCGTATTAGGTAATGGAATGGTAATTGATCCAAAAGCTTTTGTT
GAAGAAGTTGCGTATCTTCATGAACGTAATGTATCGACGGATAATCTACGTATAAGTAATCGTGCTCATGTTATCCTTCC
ATATCATTTGAAACTGGATATTCTTCAAGAAGAAGACAAAGGTGCAAATAAAATTGGAACAACGAAAAAAGGAATTGGCC
CTGCATATATGGATAAAGCGGCACGTGTCGGTATTCGTGTAGCGGATTTGATGGATAAAGATGCTTTCCGTGAAAAACTA
GTACAAAATTTAAAAGAAAAGAATCGTCTATTTGAAAAGGTTTATGAAGCAGATCCTATTCAAGTAGAAGAAATTTTAGA
TGAATATTATGCGTATGGCCAGAAGATGGCACCTTACGTAACGGATACTTCGGTAGTATTAAATGATGCGATTGATGAAG
GTAAGCGTGTGTTATTTGAAGGTGCACAAGGCGTTATGCTAGATATTGACCAAGGAACATATCCGTTTGTTACTTCCTCC
AACCCAATTGCTGGTGGAGTAACGATTGGATCTGGAGTAGGACCTACAAAAATTAATCACGTCGTTGGTGTATCCAAAGC
ATATACAACTCGTGTAGGAGATGGACCATTCCCGACTGAGCTTCATGATGAGATCGGTAATCAAATTCGTGAAGTTGGAC
GTGAATATGGTACTACGACTGGACGCGCTCGTCGTGTTGGTTGGTTTGATAGTGTTGTTGTTCGTCATGCTCGTCGAGTA
AGTGGTATAACGGACTTATCTTTAAACTCTATTGACGTATTAACAGGTATTGAAACACTGAAAATCTGTGTTGCCTATCG
TTATAAAGGGGAAATCATGAAAGAGTTCCCAGCAAGCTTAAAAGTCCTTGCAGAATGTGAACCGGTATATGAAGAAATGC
CAGGTTGGCAAGAAGATATTACAGGAGCGAAGAGCTTAGACGATCTTCCGGAGAATGCACGTCACTATTTGGAAAGAGTA
TCTCAATTAACAGAGATTCCGTTATCTATTTTCTCTGTTGGACCAGATCGTTCTCAAACAAATGTTGTACGTAGCGTGTA
TCGTCCATAA

Upstream 100 bases:

>100_bases
TTTTACACATATATTTTTCAGAACCAAGAGTGGACGAATAATACGAATATAAGTCGCTATGAGAATTAAGTATATAATTA
ATTCATTATGGAGGTGCACT

Downstream 100 bases:

>100_bases
TAGAATAAACAAAACGATTTAACAAAGATCACTTAAGAAGCTGTAGCCAATCTAGTTGTATTGGTTATAGCTTCTTTTTG
AGTATACTTCATTTACATTT

Product: adenylosuccinate synthetase

Products: NA

Alternate protein names: AMPSase; AdSS; IMP--aspartate ligase

Number of amino acids: Translated: 429; Mature: 428

Protein sequence:

>429_residues
MSSVVVVGTQWGDEGKGKITDFLSQNAEVVARYQGGNNAGHTIKFDDVTYKLHLIPSGIFFEDKICVLGNGMVIDPKAFV
EEVAYLHERNVSTDNLRISNRAHVILPYHLKLDILQEEDKGANKIGTTKKGIGPAYMDKAARVGIRVADLMDKDAFREKL
VQNLKEKNRLFEKVYEADPIQVEEILDEYYAYGQKMAPYVTDTSVVLNDAIDEGKRVLFEGAQGVMLDIDQGTYPFVTSS
NPIAGGVTIGSGVGPTKINHVVGVSKAYTTRVGDGPFPTELHDEIGNQIREVGREYGTTTGRARRVGWFDSVVVRHARRV
SGITDLSLNSIDVLTGIETLKICVAYRYKGEIMKEFPASLKVLAECEPVYEEMPGWQEDITGAKSLDDLPENARHYLERV
SQLTEIPLSIFSVGPDRSQTNVVRSVYRP

Sequences:

>Translated_429_residues
MSSVVVVGTQWGDEGKGKITDFLSQNAEVVARYQGGNNAGHTIKFDDVTYKLHLIPSGIFFEDKICVLGNGMVIDPKAFV
EEVAYLHERNVSTDNLRISNRAHVILPYHLKLDILQEEDKGANKIGTTKKGIGPAYMDKAARVGIRVADLMDKDAFREKL
VQNLKEKNRLFEKVYEADPIQVEEILDEYYAYGQKMAPYVTDTSVVLNDAIDEGKRVLFEGAQGVMLDIDQGTYPFVTSS
NPIAGGVTIGSGVGPTKINHVVGVSKAYTTRVGDGPFPTELHDEIGNQIREVGREYGTTTGRARRVGWFDSVVVRHARRV
SGITDLSLNSIDVLTGIETLKICVAYRYKGEIMKEFPASLKVLAECEPVYEEMPGWQEDITGAKSLDDLPENARHYLERV
SQLTEIPLSIFSVGPDRSQTNVVRSVYRP
>Mature_428_residues
SSVVVVGTQWGDEGKGKITDFLSQNAEVVARYQGGNNAGHTIKFDDVTYKLHLIPSGIFFEDKICVLGNGMVIDPKAFVE
EVAYLHERNVSTDNLRISNRAHVILPYHLKLDILQEEDKGANKIGTTKKGIGPAYMDKAARVGIRVADLMDKDAFREKLV
QNLKEKNRLFEKVYEADPIQVEEILDEYYAYGQKMAPYVTDTSVVLNDAIDEGKRVLFEGAQGVMLDIDQGTYPFVTSSN
PIAGGVTIGSGVGPTKINHVVGVSKAYTTRVGDGPFPTELHDEIGNQIREVGREYGTTTGRARRVGWFDSVVVRHARRVS
GITDLSLNSIDVLTGIETLKICVAYRYKGEIMKEFPASLKVLAECEPVYEEMPGWQEDITGAKSLDDLPENARHYLERVS
QLTEIPLSIFSVGPDRSQTNVVRSVYRP

Specific function: Plays an important role in the de novo pathway of purine nucleotide biosynthesis. Catalyzes the first commited step in the biosynthesis of AMP from IMP

COG id: COG0104

COG function: function code F; Adenylosuccinate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the adenylosuccinate synthetase family

Homologues:

Organism=Homo sapiens, GI34577063, Length=426, Percent_Identity=43.1924882629108, Blast_Score=342, Evalue=5e-94,
Organism=Homo sapiens, GI22748717, Length=420, Percent_Identity=41.9047619047619, Blast_Score=328, Evalue=4e-90,
Organism=Homo sapiens, GI40316944, Length=391, Percent_Identity=40.920716112532, Blast_Score=288, Evalue=5e-78,
Organism=Escherichia coli, GI1790620, Length=428, Percent_Identity=46.4953271028037, Blast_Score=402, Evalue=1e-113,
Organism=Caenorhabditis elegans, GI25146553, Length=421, Percent_Identity=41.5676959619952, Blast_Score=330, Evalue=1e-90,
Organism=Caenorhabditis elegans, GI25146555, Length=421, Percent_Identity=41.5676959619952, Blast_Score=329, Evalue=1e-90,
Organism=Saccharomyces cerevisiae, GI6324109, Length=421, Percent_Identity=41.3301662707838, Blast_Score=333, Evalue=4e-92,
Organism=Drosophila melanogaster, GI21356033, Length=417, Percent_Identity=42.9256594724221, Blast_Score=338, Evalue=4e-93,

Paralogues:

None

Copy number: 940 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): PURA_OCEIH (Q8EKX9)

Other databases:

- EMBL:   BA000028
- RefSeq:   NP_694375.1
- ProteinModelPortal:   Q8EKX9
- SMR:   Q8EKX9
- GeneID:   1016195
- GenomeReviews:   BA000028_GR
- KEGG:   oih:OB3453
- NMPDR:   fig|221109.1.peg.3457
- HOGENOM:   HBG658237
- OMA:   DYVVRYQ
- ProtClustDB:   PRK01117
- BioCyc:   OIHE221109:OB3453-MONOMER
- BRENDA:   6.3.4.4
- GO:   GO:0005737
- HAMAP:   MF_00011
- InterPro:   IPR018220
- InterPro:   IPR001114
- PANTHER:   PTHR11846
- SMART:   SM00788
- TIGRFAMs:   TIGR00184

Pfam domain/function: PF00709 Adenylsucc_synt

EC number: =6.3.4.4

Molecular weight: Translated: 47579; Mature: 47447

Theoretical pI: Translated: 5.37; Mature: 5.37

Prosite motif: PS01266 ADENYLOSUCCIN_SYN_1; PS00513 ADENYLOSUCCIN_SYN_2

Important sites: ACT_SITE 13-13 ACT_SITE 41-41 BINDING 128-128 BINDING 142-142 BINDING 223-223 BINDING 238-238 BINDING 302-302 BINDING 304-304

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSVVVVGTQWGDEGKGKITDFLSQNAEVVARYQGGNNAGHTIKFDDVTYKLHLIPSGIF
CCCEEEEEECCCCCCCCHHHHHHCCCCEEEEEEECCCCCCCEEEECCEEEEEEEEECCCE
FEDKICVLGNGMVIDPKAFVEEVAYLHERNVSTDNLRISNRAHVILPYHLKLDILQEEDK
ECCCEEEEECCEEECHHHHHHHHHHHHHCCCCCCCEEECCCEEEEEEEEEEEEEEECCCC
GANKIGTTKKGIGPAYMDKAARVGIRVADLMDKDAFREKLVQNLKEKNRLFEKVYEADPI
CCCCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
QVEEILDEYYAYGQKMAPYVTDTSVVLNDAIDEGKRVLFEGAQGVMLDIDQGTYPFVTSS
CHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCEEEECCCEEEEEECCCCCCEEECC
NPIAGGVTIGSGVGPTKINHVVGVSKAYTTRVGDGPFPTELHDEIGNQIREVGREYGTTT
CCCCCCEEECCCCCCCHHHHHHCCCHHHEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCC
GRARRVGWFDSVVVRHARRVSGITDLSLNSIDVLTGIETLKICVAYRYKGEIMKEFPASL
CCEEECCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHH
KVLAECEPVYEEMPGWQEDITGAKSLDDLPENARHYLERVSQLTEIPLSIFSVGPDRSQT
HHHHHCCHHHHHCCCCHHHCCCCHHHHCCHHHHHHHHHHHHHHHHCCHHEEECCCCCCHH
NVVRSVYRP
HHHHHHCCC
>Mature Secondary Structure 
SSVVVVGTQWGDEGKGKITDFLSQNAEVVARYQGGNNAGHTIKFDDVTYKLHLIPSGIF
CCEEEEEECCCCCCCCHHHHHHCCCCEEEEEEECCCCCCCEEEECCEEEEEEEEECCCE
FEDKICVLGNGMVIDPKAFVEEVAYLHERNVSTDNLRISNRAHVILPYHLKLDILQEEDK
ECCCEEEEECCEEECHHHHHHHHHHHHHCCCCCCCEEECCCEEEEEEEEEEEEEEECCCC
GANKIGTTKKGIGPAYMDKAARVGIRVADLMDKDAFREKLVQNLKEKNRLFEKVYEADPI
CCCCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
QVEEILDEYYAYGQKMAPYVTDTSVVLNDAIDEGKRVLFEGAQGVMLDIDQGTYPFVTSS
CHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCEEEECCCEEEEEECCCCCCEEECC
NPIAGGVTIGSGVGPTKINHVVGVSKAYTTRVGDGPFPTELHDEIGNQIREVGREYGTTT
CCCCCCEEECCCCCCCHHHHHHCCCHHHEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCC
GRARRVGWFDSVVVRHARRVSGITDLSLNSIDVLTGIETLKICVAYRYKGEIMKEFPASL
CCEEECCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHH
KVLAECEPVYEEMPGWQEDITGAKSLDDLPENARHYLERVSQLTEIPLSIFSVGPDRSQT
HHHHHCCHHHHHCCCCHHHCCCCHHHHCCHHHHHHHHHHHHHHHHCCHHEEECCCCCCHH
NVVRSVYRP
HHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12235376