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The map label for this gene is leuB

Identifier: 23100074

GI number: 23100074

Start: 2682076

End: 2683176

Strand: Reverse

Name: leuB

Synonym: OB2619

Alternate gene names: 23100074

Gene position: 2683176-2682076 (Counterclockwise)

Preceding gene: 23100075

Following gene: 23100073

Centisome position: 73.91

GC content: 38.96

Gene sequence:

>1101_bases
ATGGAGAAACATATTATTTTGCTTCCTGGTGATGGAATAGGTAGAGAGATTATTGATTCTGCTAAACAAGTATTAACAGC
AATAGCAAGCGAATATAATCATCGATTTACATTTGAAGAGCATGCAATTGGTGGAAGTGCTATTGATGAATATGGTACAC
CATTGCCTGATAAAACAGTAGATGCCTGTTCGAAAGCAGATGCAATTTTATTGGGAGCTGTTGGTGGTCCTAAATGGGAT
GCCAACCCGTCTCATTTAAGACCAGAAAAAGGATTGCTAGGGATACGAAAACAACTTGGACTTTTTGCCAATTTACGACC
AGTTAAAACAATTTCTTCCTTATTATATGCGTCACCATTAAAAGAAGAAATTGTAAGTCAGGCAGATATGTTAATCATTC
GCGAGTTAACTGGAGGAATTTATTTTGGCACACCTAGTGAACGAACAACTAATGGCGTAGTAGATACACTTCAGTACTCA
CGAGAAGAAATAGAACGAATTGTCGAGCGCGGATTCGAAGCTGCAAGAATTCGAAAAAAACATCTTACGTCCGTTGATAA
GGCAAATGTACTAGAGTCTAGCAAACTATGGAGAGAAATCGTGGAAGAAAAGTCGAAAGATTATCCTGATGTAGCTGTAA
ACCATCTACTTGTCGATGCAGCTGCAATGAAGCTTGTTACACAGCCGAGTTTCTTTGATGTAATCGTAACGGAGAATTTG
TTTGGAGACATATTGAGTGATGAAGCATCCGTACTCACCGGGTCTTTAGGAATGCTGCCATCAGCGAGTTTACGTGCTGA
TGGACTTGGCTTATATGAACCTGTACACGGGTCAGCTCCTGATATCGCAGGAAAAGGAATCGCTAATCCATTAGCAATGA
TTTTATCTGCTGCATTAATGTTAGAGCATTCTTTTGGCTTAGTAGATGAAGCGAAGGAAATAGAGCGAGCGGTAAATGAT
TGTTTATTACAAGGTTTTCACACAGCAGATATTCATATACCAGGTGGAGTTCAAGTTGGTACAAAGCAAATGACAGACGC
AGTTTTGGAAAATGTAACAACAAAAAGTATTTCAGATTCTATATGTAGCTCGTATGTATAG

Upstream 100 bases:

>100_bases
TCTGCAGTAGCATTCATTCAGGCAGTAAATCGTTATTACGTTCAAAAGAAAGCAAATCTAACTCGACTTGTCTATGAGTC
ATAAAAGGAGGAAATAAGAA

Downstream 100 bases:

>100_bases
GATGGAGGTGAAAGACGTGCAAAAACCACAAACTATTTTCGAAAAGATATGGGAAAATCATATGGTTCATGAAGAAGAGG
GGAAACCAAGTCTTCTTTAT

Product: 3-isopropylmalate dehydrogenase

Products: NA

Alternate protein names: 3-IPM-DH; Beta-IPM dehydrogenase; IMDH

Number of amino acids: Translated: 366; Mature: 366

Protein sequence:

>366_residues
MEKHIILLPGDGIGREIIDSAKQVLTAIASEYNHRFTFEEHAIGGSAIDEYGTPLPDKTVDACSKADAILLGAVGGPKWD
ANPSHLRPEKGLLGIRKQLGLFANLRPVKTISSLLYASPLKEEIVSQADMLIIRELTGGIYFGTPSERTTNGVVDTLQYS
REEIERIVERGFEAARIRKKHLTSVDKANVLESSKLWREIVEEKSKDYPDVAVNHLLVDAAAMKLVTQPSFFDVIVTENL
FGDILSDEASVLTGSLGMLPSASLRADGLGLYEPVHGSAPDIAGKGIANPLAMILSAALMLEHSFGLVDEAKEIERAVND
CLLQGFHTADIHIPGGVQVGTKQMTDAVLENVTTKSISDSICSSYV

Sequences:

>Translated_366_residues
MEKHIILLPGDGIGREIIDSAKQVLTAIASEYNHRFTFEEHAIGGSAIDEYGTPLPDKTVDACSKADAILLGAVGGPKWD
ANPSHLRPEKGLLGIRKQLGLFANLRPVKTISSLLYASPLKEEIVSQADMLIIRELTGGIYFGTPSERTTNGVVDTLQYS
REEIERIVERGFEAARIRKKHLTSVDKANVLESSKLWREIVEEKSKDYPDVAVNHLLVDAAAMKLVTQPSFFDVIVTENL
FGDILSDEASVLTGSLGMLPSASLRADGLGLYEPVHGSAPDIAGKGIANPLAMILSAALMLEHSFGLVDEAKEIERAVND
CLLQGFHTADIHIPGGVQVGTKQMTDAVLENVTTKSISDSICSSYV
>Mature_366_residues
MEKHIILLPGDGIGREIIDSAKQVLTAIASEYNHRFTFEEHAIGGSAIDEYGTPLPDKTVDACSKADAILLGAVGGPKWD
ANPSHLRPEKGLLGIRKQLGLFANLRPVKTISSLLYASPLKEEIVSQADMLIIRELTGGIYFGTPSERTTNGVVDTLQYS
REEIERIVERGFEAARIRKKHLTSVDKANVLESSKLWREIVEEKSKDYPDVAVNHLLVDAAAMKLVTQPSFFDVIVTENL
FGDILSDEASVLTGSLGMLPSASLRADGLGLYEPVHGSAPDIAGKGIANPLAMILSAALMLEHSFGLVDEAKEIERAVND
CLLQGFHTADIHIPGGVQVGTKQMTDAVLENVTTKSISDSICSSYV

Specific function: Catalyzes the oxidation of 3-carboxy-2-hydroxy-4- methylpentanoate (3-isopropylmalate) to 3-carboxy-4-methyl-2- oxopentanoate. The product decarboxylates to 4-methyl-2 oxopentanoate

COG id: COG0473

COG function: function code CE; Isocitrate/isopropylmalate dehydrogenase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isocitrate and isopropylmalate dehydrogenases family. LeuB type 1 subfamily

Homologues:

Organism=Homo sapiens, GI5031777, Length=319, Percent_Identity=35.1097178683386, Blast_Score=161, Evalue=1e-39,
Organism=Homo sapiens, GI28178821, Length=306, Percent_Identity=29.0849673202614, Blast_Score=117, Evalue=2e-26,
Organism=Homo sapiens, GI28178816, Length=306, Percent_Identity=29.0849673202614, Blast_Score=117, Evalue=2e-26,
Organism=Homo sapiens, GI4758582, Length=348, Percent_Identity=27.8735632183908, Blast_Score=106, Evalue=3e-23,
Organism=Homo sapiens, GI28178838, Length=317, Percent_Identity=28.391167192429, Blast_Score=103, Evalue=2e-22,
Organism=Homo sapiens, GI28178819, Length=159, Percent_Identity=32.0754716981132, Blast_Score=89, Evalue=6e-18,
Organism=Escherichia coli, GI87081683, Length=356, Percent_Identity=51.4044943820225, Blast_Score=354, Evalue=5e-99,
Organism=Escherichia coli, GI1788101, Length=367, Percent_Identity=34.8773841961853, Blast_Score=192, Evalue=4e-50,
Organism=Escherichia coli, GI1787381, Length=400, Percent_Identity=24.25, Blast_Score=88, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI71986051, Length=335, Percent_Identity=32.2388059701493, Blast_Score=147, Evalue=9e-36,
Organism=Caenorhabditis elegans, GI17550882, Length=355, Percent_Identity=30.9859154929577, Blast_Score=129, Evalue=2e-30,
Organism=Caenorhabditis elegans, GI17505779, Length=353, Percent_Identity=31.1614730878187, Blast_Score=126, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI25144293, Length=336, Percent_Identity=30.952380952381, Blast_Score=120, Evalue=9e-28,
Organism=Saccharomyces cerevisiae, GI6319830, Length=365, Percent_Identity=49.3150684931507, Blast_Score=305, Evalue=5e-84,
Organism=Saccharomyces cerevisiae, GI6322097, Length=365, Percent_Identity=38.6301369863014, Blast_Score=189, Evalue=8e-49,
Organism=Saccharomyces cerevisiae, GI6324709, Length=353, Percent_Identity=34.2776203966006, Blast_Score=154, Evalue=2e-38,
Organism=Saccharomyces cerevisiae, GI6324291, Length=358, Percent_Identity=31.5642458100559, Blast_Score=142, Evalue=7e-35,
Organism=Drosophila melanogaster, GI24643270, Length=337, Percent_Identity=32.3442136498516, Blast_Score=155, Evalue=3e-38,
Organism=Drosophila melanogaster, GI24643268, Length=337, Percent_Identity=32.3442136498516, Blast_Score=155, Evalue=4e-38,
Organism=Drosophila melanogaster, GI24661184, Length=359, Percent_Identity=30.6406685236769, Blast_Score=135, Evalue=3e-32,
Organism=Drosophila melanogaster, GI161078633, Length=353, Percent_Identity=28.328611898017, Blast_Score=109, Evalue=3e-24,
Organism=Drosophila melanogaster, GI24650122, Length=353, Percent_Identity=28.328611898017, Blast_Score=109, Evalue=3e-24,
Organism=Drosophila melanogaster, GI161078637, Length=353, Percent_Identity=28.328611898017, Blast_Score=108, Evalue=4e-24,
Organism=Drosophila melanogaster, GI281362242, Length=354, Percent_Identity=28.2485875706215, Blast_Score=108, Evalue=4e-24,
Organism=Drosophila melanogaster, GI24648872, Length=354, Percent_Identity=28.2485875706215, Blast_Score=108, Evalue=4e-24,
Organism=Drosophila melanogaster, GI161078635, Length=353, Percent_Identity=28.328611898017, Blast_Score=108, Evalue=4e-24,
Organism=Drosophila melanogaster, GI161078639, Length=351, Percent_Identity=28.4900284900285, Blast_Score=108, Evalue=6e-24,
Organism=Drosophila melanogaster, GI20130355, Length=334, Percent_Identity=25.748502994012, Blast_Score=84, Evalue=2e-16,

Paralogues:

None

Copy number: 400 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): LEU3_OCEIH (Q8EN68)

Other databases:

- EMBL:   BA000028
- RefSeq:   NP_693540.1
- ProteinModelPortal:   Q8EN68
- SMR:   Q8EN68
- GeneID:   1016601
- GenomeReviews:   BA000028_GR
- KEGG:   oih:OB2619
- NMPDR:   fig|221109.1.peg.2618
- HOGENOM:   HBG518924
- OMA:   MSYQIAV
- ProtClustDB:   PRK00772
- BioCyc:   OIHE221109:OB2619-MONOMER
- BRENDA:   1.1.1.85
- GO:   GO:0005737
- HAMAP:   MF_01033
- InterPro:   IPR019818
- InterPro:   IPR001804
- InterPro:   IPR004429
- Gene3D:   G3DSA:3.40.718.10
- PANTHER:   PTHR11835
- PANTHER:   PTHR11835:SF13
- TIGRFAMs:   TIGR00169

Pfam domain/function: PF00180 Iso_dh

EC number: =1.1.1.85

Molecular weight: Translated: 39545; Mature: 39545

Theoretical pI: Translated: 4.88; Mature: 4.88

Prosite motif: PS00470 IDH_IMDH

Important sites: BINDING 96-96 BINDING 106-106 BINDING 134-134 BINDING 219-219

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKHIILLPGDGIGREIIDSAKQVLTAIASEYNHRFTFEEHAIGGSAIDEYGTPLPDKTV
CCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHCCCCCHHHHCCCCCCCHHH
DACSKADAILLGAVGGPKWDANPSHLRPEKGLLGIRKQLGLFANLRPVKTISSLLYASPL
HHHHCCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHCHH
KEEIVSQADMLIIRELTGGIYFGTPSERTTNGVVDTLQYSREEIERIVERGFEAARIRKK
HHHHHHHHHHHHHHHHHCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
HLTSVDKANVLESSKLWREIVEEKSKDYPDVAVNHLLVDAAAMKLVTQPSFFDVIVTENL
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCHHEEEEHHHH
FGDILSDEASVLTGSLGMLPSASLRADGLGLYEPVHGSAPDIAGKGIANPLAMILSAALM
HHHHHCCCHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHH
LEHSFGLVDEAKEIERAVNDCLLQGFHTADIHIPGGVQVGTKQMTDAVLENVTTKSISDS
HHHHCCCHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHH
ICSSYV
HHHCCC
>Mature Secondary Structure
MEKHIILLPGDGIGREIIDSAKQVLTAIASEYNHRFTFEEHAIGGSAIDEYGTPLPDKTV
CCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHCCCCCHHHHCCCCCCCHHH
DACSKADAILLGAVGGPKWDANPSHLRPEKGLLGIRKQLGLFANLRPVKTISSLLYASPL
HHHHCCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHCHH
KEEIVSQADMLIIRELTGGIYFGTPSERTTNGVVDTLQYSREEIERIVERGFEAARIRKK
HHHHHHHHHHHHHHHHHCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
HLTSVDKANVLESSKLWREIVEEKSKDYPDVAVNHLLVDAAAMKLVTQPSFFDVIVTENL
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCHHEEEEHHHH
FGDILSDEASVLTGSLGMLPSASLRADGLGLYEPVHGSAPDIAGKGIANPLAMILSAALM
HHHHHCCCHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHH
LEHSFGLVDEAKEIERAVNDCLLQGFHTADIHIPGGVQVGTKQMTDAVLENVTTKSISDS
HHHHCCCHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHH
ICSSYV
HHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12235376