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The map label for this gene is leuC

Identifier: 23100073

GI number: 23100073

Start: 2680653

End: 2682059

Strand: Reverse

Name: leuC

Synonym: OB2618

Alternate gene names: 23100073

Gene position: 2682059-2680653 (Counterclockwise)

Preceding gene: 23100074

Following gene: 23100072

Centisome position: 73.88

GC content: 39.37

Gene sequence:

>1407_bases
GTGCAAAAACCACAAACTATTTTCGAAAAGATATGGGAAAATCATATGGTTCATGAAGAAGAGGGGAAACCAAGTCTTCT
TTATATTGATCAACATTTGGTTCATGAAGTAACTTCTCCACAAGCTTTTGAGGGGCTTCGTTTAAATAATCGAAAGGTTC
GTCGTCCTGACTTAACATTTGCAACGATGGATCATAATGTCCCAACTGTAAATAGGGAGTCATTTAAAGATGAAATTTCC
AAAAAGCAGATGGACGCACTGAAGAAAAATTGTGAGGAATTTGGTATAAAGCTTGCTGATATGTACCATCCAGATCAGGG
GATTGTACACGTAATTGGCCCGCAACTTGGTCTTACCCAACCAGGGAAAACGATTGTATGTGGGGATAGTCATACTTCAA
CACATGGAGCATTTGGTGCTTTAGCATTTGGAATAGGTACAAGTGAGGTCGAACATGTTTTAGCAACACAAACTATTTGG
CAAGACAAACCAAAAACCTTAAATGTCAAAGTTATAGGTGAACTAGGCATAGGGGTAACTGCAAAAGATTTAATTTTAGC
GATTATTGCAAAGTTTGGTGTTCAATTTGGTACTGGATATGTAATGGAATATACAGGTGAGTCCATTCGTAAAATGTCCA
TGGAAGAAAGAATGACAATCTGTAATATGTCGATTGAAGCGGGTGCGAGAGCAGGTTTAATTAGCCCTGATCAAACTACA
GTAGACTACTTACGTGGACGTGAAATGGTTCCAAAAGGAGAAGCATATGATACTTTAGCAGCTGAATGGTTACAATTAGC
TACGGATGAAGATGCTGTCTATGATGATACGGTTACTATTCATGCTAATGAAATTGAGCCTCAAGTTACATGGGGAACAA
ATCCAGGTATGTGTGTACCGATAAGCTCTTCTACACCATCTATTGAAAAAGCAAGTTATCCAGACGAAGTAGAACGTGCG
CTTGAATATATGGGGTTAACAGAAAATCAATTAATGACTTCTATTGAAGTAGACCATGTATTTATCGGATCATGTACGAA
CTCGAGACTAAGTGATTTGAAAAAAGCAGCAGCAATTGTGAAGGGTAAAAAAGTGAAAGCTGGTATTCGTGCTATGGTAG
TGCCCGGTTCATTTCTTGTAAAGCAGAAAGCAGAGGAAATAGGGTTAGATCAAGTATTTATGGATGCTGGTTTTGAATGG
AGAAATTCTGGATGTAGTATGTGTCTAGGAATGAATGATGATATTGTGCCAGCTGGAGGAAGATGTGCTTCAACTTCCAA
CAGAAATTTTGAAGGTAGACAAGGAAACGGGGCTAGAACACATCTCGTCAGTCCAGAAATGGCTGCAGCTGCGGCAATAG
AAGGACACTTTGTTGATGTTAGATCCTACGCTAGCGTCCCGAGTTAG

Upstream 100 bases:

>100_bases
GGTACAAAGCAAATGACAGACGCAGTTTTGGAAAATGTAACAACAAAAAGTATTTCAGATTCTATATGTAGCTCGTATGT
ATAGGATGGAGGTGAAAGAC

Downstream 100 bases:

>100_bases
GAGGAGATCTTAAATGGAACCATTGGAATTTCATAGTGGAAAGGTCTGTCCATTAAATAGAGCTAATGTAGACACGGACC
AAATTATTCCAAAGCAATTT

Product: isopropylmalate isomerase large subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase; IPMI; Isopropylmalate isomerase

Number of amino acids: Translated: 468; Mature: 468

Protein sequence:

>468_residues
MQKPQTIFEKIWENHMVHEEEGKPSLLYIDQHLVHEVTSPQAFEGLRLNNRKVRRPDLTFATMDHNVPTVNRESFKDEIS
KKQMDALKKNCEEFGIKLADMYHPDQGIVHVIGPQLGLTQPGKTIVCGDSHTSTHGAFGALAFGIGTSEVEHVLATQTIW
QDKPKTLNVKVIGELGIGVTAKDLILAIIAKFGVQFGTGYVMEYTGESIRKMSMEERMTICNMSIEAGARAGLISPDQTT
VDYLRGREMVPKGEAYDTLAAEWLQLATDEDAVYDDTVTIHANEIEPQVTWGTNPGMCVPISSSTPSIEKASYPDEVERA
LEYMGLTENQLMTSIEVDHVFIGSCTNSRLSDLKKAAAIVKGKKVKAGIRAMVVPGSFLVKQKAEEIGLDQVFMDAGFEW
RNSGCSMCLGMNDDIVPAGGRCASTSNRNFEGRQGNGARTHLVSPEMAAAAAIEGHFVDVRSYASVPS

Sequences:

>Translated_468_residues
MQKPQTIFEKIWENHMVHEEEGKPSLLYIDQHLVHEVTSPQAFEGLRLNNRKVRRPDLTFATMDHNVPTVNRESFKDEIS
KKQMDALKKNCEEFGIKLADMYHPDQGIVHVIGPQLGLTQPGKTIVCGDSHTSTHGAFGALAFGIGTSEVEHVLATQTIW
QDKPKTLNVKVIGELGIGVTAKDLILAIIAKFGVQFGTGYVMEYTGESIRKMSMEERMTICNMSIEAGARAGLISPDQTT
VDYLRGREMVPKGEAYDTLAAEWLQLATDEDAVYDDTVTIHANEIEPQVTWGTNPGMCVPISSSTPSIEKASYPDEVERA
LEYMGLTENQLMTSIEVDHVFIGSCTNSRLSDLKKAAAIVKGKKVKAGIRAMVVPGSFLVKQKAEEIGLDQVFMDAGFEW
RNSGCSMCLGMNDDIVPAGGRCASTSNRNFEGRQGNGARTHLVSPEMAAAAAIEGHFVDVRSYASVPS
>Mature_468_residues
MQKPQTIFEKIWENHMVHEEEGKPSLLYIDQHLVHEVTSPQAFEGLRLNNRKVRRPDLTFATMDHNVPTVNRESFKDEIS
KKQMDALKKNCEEFGIKLADMYHPDQGIVHVIGPQLGLTQPGKTIVCGDSHTSTHGAFGALAFGIGTSEVEHVLATQTIW
QDKPKTLNVKVIGELGIGVTAKDLILAIIAKFGVQFGTGYVMEYTGESIRKMSMEERMTICNMSIEAGARAGLISPDQTT
VDYLRGREMVPKGEAYDTLAAEWLQLATDEDAVYDDTVTIHANEIEPQVTWGTNPGMCVPISSSTPSIEKASYPDEVERA
LEYMGLTENQLMTSIEVDHVFIGSCTNSRLSDLKKAAAIVKGKKVKAGIRAMVVPGSFLVKQKAEEIGLDQVFMDAGFEW
RNSGCSMCLGMNDDIVPAGGRCASTSNRNFEGRQGNGARTHLVSPEMAAAAAIEGHFVDVRSYASVPS

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate

COG id: COG0065

COG function: function code E; 3-isopropylmalate dehydratase large subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aconitase/IPM isomerase family. LeuC type 1 subfamily

Homologues:

Organism=Homo sapiens, GI4501867, Length=367, Percent_Identity=29.700272479564, Blast_Score=120, Evalue=2e-27,
Organism=Homo sapiens, GI8659555, Length=448, Percent_Identity=24.7767857142857, Blast_Score=101, Evalue=1e-21,
Organism=Homo sapiens, GI41352693, Length=367, Percent_Identity=25.8855585831063, Blast_Score=90, Evalue=4e-18,
Organism=Escherichia coli, GI1786259, Length=464, Percent_Identity=59.2672413793103, Blast_Score=562, Evalue=1e-161,
Organism=Escherichia coli, GI1787531, Length=364, Percent_Identity=27.1978021978022, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI87081781, Length=387, Percent_Identity=24.031007751938, Blast_Score=79, Evalue=7e-16,
Organism=Escherichia coli, GI2367097, Length=364, Percent_Identity=23.0769230769231, Blast_Score=64, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI25149337, Length=392, Percent_Identity=29.0816326530612, Blast_Score=129, Evalue=2e-30,
Organism=Caenorhabditis elegans, GI32564738, Length=392, Percent_Identity=29.0816326530612, Blast_Score=129, Evalue=3e-30,
Organism=Caenorhabditis elegans, GI25149342, Length=310, Percent_Identity=30, Blast_Score=120, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI17568399, Length=462, Percent_Identity=26.4069264069264, Blast_Score=114, Evalue=1e-25,
Organism=Saccharomyces cerevisiae, GI6321429, Length=471, Percent_Identity=56.4755838641189, Blast_Score=528, Evalue=1e-151,
Organism=Saccharomyces cerevisiae, GI6320440, Length=453, Percent_Identity=27.1523178807947, Blast_Score=150, Evalue=4e-37,
Organism=Saccharomyces cerevisiae, GI6322261, Length=488, Percent_Identity=28.2786885245902, Blast_Score=149, Evalue=7e-37,
Organism=Saccharomyces cerevisiae, GI6323335, Length=388, Percent_Identity=29.3814432989691, Blast_Score=144, Evalue=2e-35,
Organism=Drosophila melanogaster, GI281365315, Length=391, Percent_Identity=29.923273657289, Blast_Score=135, Evalue=5e-32,
Organism=Drosophila melanogaster, GI17864292, Length=391, Percent_Identity=29.923273657289, Blast_Score=135, Evalue=5e-32,
Organism=Drosophila melanogaster, GI161076999, Length=391, Percent_Identity=29.923273657289, Blast_Score=135, Evalue=6e-32,
Organism=Drosophila melanogaster, GI28571643, Length=389, Percent_Identity=29.305912596401, Blast_Score=122, Evalue=4e-28,
Organism=Drosophila melanogaster, GI17137564, Length=462, Percent_Identity=25.974025974026, Blast_Score=89, Evalue=5e-18,
Organism=Drosophila melanogaster, GI24645686, Length=460, Percent_Identity=26.9565217391304, Blast_Score=87, Evalue=3e-17,

Paralogues:

None

Copy number: 280 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): LEUC_OCEIH (Q8EN69)

Other databases:

- EMBL:   BA000028
- RefSeq:   NP_693539.1
- ProteinModelPortal:   Q8EN69
- SMR:   Q8EN69
- GeneID:   1017086
- GenomeReviews:   BA000028_GR
- KEGG:   oih:OB2618
- NMPDR:   fig|221109.1.peg.2617
- HOGENOM:   HBG330745
- OMA:   RPHAPKG
- ProtClustDB:   PRK05478
- BioCyc:   OIHE221109:OB2618-MONOMER
- BRENDA:   4.2.1.33
- HAMAP:   MF_01026
- InterPro:   IPR004430
- InterPro:   IPR015931
- InterPro:   IPR015937
- InterPro:   IPR001030
- InterPro:   IPR015932
- InterPro:   IPR018136
- InterPro:   IPR015936
- Gene3D:   G3DSA:3.30.499.10
- Gene3D:   G3DSA:3.40.1060.10
- PANTHER:   PTHR11670
- PANTHER:   PTHR11670:SF6
- PRINTS:   PR00415
- TIGRFAMs:   TIGR00170

Pfam domain/function: PF00330 Aconitase; SSF53732 Aconitase_N

EC number: =4.2.1.33

Molecular weight: Translated: 51299; Mature: 51299

Theoretical pI: Translated: 5.34; Mature: 5.34

Prosite motif: PS00450 ACONITASE_1; PS01244 ACONITASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQKPQTIFEKIWENHMVHEEEGKPSLLYIDQHLVHEVTSPQAFEGLRLNNRKVRRPDLTF
CCCHHHHHHHHHHHCCEECCCCCCCEEEECHHHHHHCCCCCHHCCCCCCCCCCCCCCCEE
ATMDHNVPTVNRESFKDEISKKQMDALKKNCEEFGIKLADMYHPDQGIVHVIGPQLGLTQ
EEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCEEEEECCCCCCCC
PGKTIVCGDSHTSTHGAFGALAFGIGTSEVEHVLATQTIWQDKPKTLNVKVIGELGIGVT
CCCEEEECCCCCCCCCCHHHHHCCCCHHHHHHHHHHHHHHCCCCCEEEEEEEEECCCCCC
AKDLILAIIAKFGVQFGTGYVMEYTGESIRKMSMEERMTICNMSIEAGARAGLISPDQTT
HHHHHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHHCCCCHHCCCCCCCCCCCCHH
VDYLRGREMVPKGEAYDTLAAEWLQLATDEDAVYDDTVTIHANEIEPQVTWGTNPGMCVP
HHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCCEECCEEEEEECCCCCEEEECCCCCEEEE
ISSSTPSIEKASYPDEVERALEYMGLTENQLMTSIEVDHVFIGSCTNSRLSDLKKAAAIV
ECCCCCCCCCCCCHHHHHHHHHHCCCCHHHHHEEEEECEEEEECCCCHHHHHHHHHHHHH
KGKKVKAGIRAMVVPGSFLVKQKAEEIGLDQVFMDAGFEWRNSGCSMCLGMNDDIVPAGG
CCCEECCCCEEEEECCHHHHHHHHHHCCHHHHHHHCCCCCCCCCCCEEECCCCCCCCCCC
RCASTSNRNFEGRQGNGARTHLVSPEMAAAAAIEGHFVDVRSYASVPS
CCCCCCCCCCCCCCCCCCEEEECCCHHHHHHHHCCEEEEHHHHCCCCC
>Mature Secondary Structure
MQKPQTIFEKIWENHMVHEEEGKPSLLYIDQHLVHEVTSPQAFEGLRLNNRKVRRPDLTF
CCCHHHHHHHHHHHCCEECCCCCCCEEEECHHHHHHCCCCCHHCCCCCCCCCCCCCCCEE
ATMDHNVPTVNRESFKDEISKKQMDALKKNCEEFGIKLADMYHPDQGIVHVIGPQLGLTQ
EEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCEEEEECCCCCCCC
PGKTIVCGDSHTSTHGAFGALAFGIGTSEVEHVLATQTIWQDKPKTLNVKVIGELGIGVT
CCCEEEECCCCCCCCCCHHHHHCCCCHHHHHHHHHHHHHHCCCCCEEEEEEEEECCCCCC
AKDLILAIIAKFGVQFGTGYVMEYTGESIRKMSMEERMTICNMSIEAGARAGLISPDQTT
HHHHHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHHCCCCHHCCCCCCCCCCCCHH
VDYLRGREMVPKGEAYDTLAAEWLQLATDEDAVYDDTVTIHANEIEPQVTWGTNPGMCVP
HHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCCEECCEEEEEECCCCCEEEECCCCCEEEE
ISSSTPSIEKASYPDEVERALEYMGLTENQLMTSIEVDHVFIGSCTNSRLSDLKKAAAIV
ECCCCCCCCCCCCHHHHHHHHHHCCCCHHHHHEEEEECEEEEECCCCHHHHHHHHHHHHH
KGKKVKAGIRAMVVPGSFLVKQKAEEIGLDQVFMDAGFEWRNSGCSMCLGMNDDIVPAGG
CCCEECCCCEEEEECCHHHHHHHHHHCCHHHHHHHCCCCCCCCCCCEEECCCCCCCCCCC
RCASTSNRNFEGRQGNGARTHLVSPEMAAAAAIEGHFVDVRSYASVPS
CCCCCCCCCCCCCCCCCCEEEECCCHHHHHHHHCCEEEEHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12235376