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The map label for this gene is leuA

Identifier: 23100075

GI number: 23100075

Start: 2683193

End: 2684740

Strand: Reverse

Name: leuA

Synonym: OB2620

Alternate gene names: 23100075

Gene position: 2684740-2683193 (Counterclockwise)

Preceding gene: 23100076

Following gene: 23100074

Centisome position: 73.95

GC content: 37.66

Gene sequence:

>1548_bases
ATGTCCCGAATTAAGATTTTCGACACAACACTACGAGATGGAGAGCAATCTCCAGGAGTTAACTTGAATAAAGCAGAAAA
GCTTGAGATAGCAAAACAACTTGAAAAGTATGGTGTGGACCGGATGGAGGCAGGGTTTCCTGCTTCCTCTAAAGGGGATT
TTGAATCGGTGAAACAAATTGCTGAAACGATCAAGTCTTCCTCCGTCATTGCGCTTGCTCGTGCGGTGAAATCTGATATT
GATATTGCTTATGAAGCGTTAAAAGGAGCAGAGAAACCTGCGATTCATATTTTCTTAGCAACATCTCCAATTCATATGAC
GTATAAATTGAAGAAATCACCAGAACAAGTGATTGAAACAGCCGTTAACATGGTCACTTATGCAAAAGAACGTTTTGATG
AAATTGAATGGTCTGCAGAGGATGCAACTCGTTCGGATTGGAATTTCTTAGCGCAAATTATTGAAAAGGTTATTGAAGCT
GGGGCAACCGTAATAAATCTTCCAGACACGGTGGGTTATACAACTCCTGAAGAATATGGGAAATTATTTCGATTTATCAA
AGAAACAGTTCCAAATATTAATCAAGTTGCTTTATCTTGTCATTGTCACAACGACCTTGGAATGGCAGTAGCTAATTCAA
TAGCAGCAGTGGAAAATGGGGCTACACAAGTAGAGGGAACGATCAATGGAATCGGTGAGAGAGCAGGAAATGCGGCTTTA
GAAGAAGTAGCTGTCGCGTTAAAAATTCGTTCTGATTATTATCCATTTGAGACAGGATTAATTCTGAAAGAGACGAAGCG
TACAAGTGATTTGGTAGCAAAGCTAACTGGTATGTATGTGCAAGCAAATAAGGCGGTTATTGGAAGAAATGCATTTTCAC
ACGAATCGGGTATCCACCAAGATGGTGTCTTAAAAAATACCGAGACATATGAGATTATTACTCCTGAAATGGTTGGTGTA
TCCTCTAACACACTATTTCTTGGAAAACATTCTGGAAGGCATGCGTTTAAAGATAAATTAAAAGAGTTTGGTGTTGAGTT
ATCAGAAGAAGAGCTTAAAACAGCATTTGAACAATTTAAATTATTAACAGACCAAAAGAAAGAAGTAACGGACGATGATT
TATATACTATTTTAATGGATATTAAGACAGATTCAACTGTAGTTGATAAATACAAATTAATTCAATTTGGAGTTTCTTAT
GATACAGCAGCCACTCCAAAAGCAAATGTCATCTTAGAATCTCCAAATGGTTCACTAATCGAGGCAACGCAACAAGGAAA
TGGTAGTGTTGAGGCGTTATATAAAACGATAAATTACTTGATTGCAGAAAATATAACACTAGTAGATTATCAAATTAATT
CGGTTGGTGGGGGGAAAGACGCCCTGGCAGAATCCCATGTTCAAATTATTATTAATGGAGAAACAATCAATGGTAGAGGG
TCTGCTCAGGATGTGCTTCAAGCGTCTGCAGTAGCATTCATTCAGGCAGTAAATCGTTATTACGTTCAAAAGAAAGCAAA
TCTAACTCGACTTGTCTATGAGTCATAA

Upstream 100 bases:

>100_bases
GAAAATAGACACCCAATTGAAACTGTGGGAAGGGAGTTACGTGAGTTAATGCCATTTGTTAAACAGCCAATTAATGCTAA
GAAAAAGGATGTGAACGTCC

Downstream 100 bases:

>100_bases
AAGGAGGAAATAAGAAATGGAGAAACATATTATTTTGCTTCCTGGTGATGGAATAGGTAGAGAGATTATTGATTCTGCTA
AACAAGTATTAACAGCAATA

Product: 2-isopropylmalate synthase

Products: NA

Alternate protein names: Alpha-IPM synthase; Alpha-isopropylmalate synthase

Number of amino acids: Translated: 515; Mature: 514

Protein sequence:

>515_residues
MSRIKIFDTTLRDGEQSPGVNLNKAEKLEIAKQLEKYGVDRMEAGFPASSKGDFESVKQIAETIKSSSVIALARAVKSDI
DIAYEALKGAEKPAIHIFLATSPIHMTYKLKKSPEQVIETAVNMVTYAKERFDEIEWSAEDATRSDWNFLAQIIEKVIEA
GATVINLPDTVGYTTPEEYGKLFRFIKETVPNINQVALSCHCHNDLGMAVANSIAAVENGATQVEGTINGIGERAGNAAL
EEVAVALKIRSDYYPFETGLILKETKRTSDLVAKLTGMYVQANKAVIGRNAFSHESGIHQDGVLKNTETYEIITPEMVGV
SSNTLFLGKHSGRHAFKDKLKEFGVELSEEELKTAFEQFKLLTDQKKEVTDDDLYTILMDIKTDSTVVDKYKLIQFGVSY
DTAATPKANVILESPNGSLIEATQQGNGSVEALYKTINYLIAENITLVDYQINSVGGGKDALAESHVQIIINGETINGRG
SAQDVLQASAVAFIQAVNRYYVQKKANLTRLVYES

Sequences:

>Translated_515_residues
MSRIKIFDTTLRDGEQSPGVNLNKAEKLEIAKQLEKYGVDRMEAGFPASSKGDFESVKQIAETIKSSSVIALARAVKSDI
DIAYEALKGAEKPAIHIFLATSPIHMTYKLKKSPEQVIETAVNMVTYAKERFDEIEWSAEDATRSDWNFLAQIIEKVIEA
GATVINLPDTVGYTTPEEYGKLFRFIKETVPNINQVALSCHCHNDLGMAVANSIAAVENGATQVEGTINGIGERAGNAAL
EEVAVALKIRSDYYPFETGLILKETKRTSDLVAKLTGMYVQANKAVIGRNAFSHESGIHQDGVLKNTETYEIITPEMVGV
SSNTLFLGKHSGRHAFKDKLKEFGVELSEEELKTAFEQFKLLTDQKKEVTDDDLYTILMDIKTDSTVVDKYKLIQFGVSY
DTAATPKANVILESPNGSLIEATQQGNGSVEALYKTINYLIAENITLVDYQINSVGGGKDALAESHVQIIINGETINGRG
SAQDVLQASAVAFIQAVNRYYVQKKANLTRLVYES
>Mature_514_residues
SRIKIFDTTLRDGEQSPGVNLNKAEKLEIAKQLEKYGVDRMEAGFPASSKGDFESVKQIAETIKSSSVIALARAVKSDID
IAYEALKGAEKPAIHIFLATSPIHMTYKLKKSPEQVIETAVNMVTYAKERFDEIEWSAEDATRSDWNFLAQIIEKVIEAG
ATVINLPDTVGYTTPEEYGKLFRFIKETVPNINQVALSCHCHNDLGMAVANSIAAVENGATQVEGTINGIGERAGNAALE
EVAVALKIRSDYYPFETGLILKETKRTSDLVAKLTGMYVQANKAVIGRNAFSHESGIHQDGVLKNTETYEIITPEMVGVS
SNTLFLGKHSGRHAFKDKLKEFGVELSEEELKTAFEQFKLLTDQKKEVTDDDLYTILMDIKTDSTVVDKYKLIQFGVSYD
TAATPKANVILESPNGSLIEATQQGNGSVEALYKTINYLIAENITLVDYQINSVGGGKDALAESHVQIIINGETINGRGS
AQDVLQASAVAFIQAVNRYYVQKKANLTRLVYES

Specific function: Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate)

COG id: COG0119

COG function: function code E; Isopropylmalate/homocitrate/citramalate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alpha-IPM synthase/homocitrate synthase family. LeuA type 1 subfamily

Homologues:

Organism=Escherichia coli, GI1786261, Length=499, Percent_Identity=46.0921843687375, Blast_Score=426, Evalue=1e-120,
Organism=Saccharomyces cerevisiae, GI6320071, Length=383, Percent_Identity=28.4595300261097, Blast_Score=164, Evalue=3e-41,
Organism=Saccharomyces cerevisiae, GI6320019, Length=372, Percent_Identity=27.9569892473118, Blast_Score=164, Evalue=4e-41,
Organism=Saccharomyces cerevisiae, GI6324225, Length=546, Percent_Identity=23.992673992674, Blast_Score=108, Evalue=3e-24,
Organism=Saccharomyces cerevisiae, GI6324682, Length=559, Percent_Identity=23.4347048300537, Blast_Score=107, Evalue=4e-24,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): LEU1_OCEIH (Q8EN67)

Other databases:

- EMBL:   BA000028
- RefSeq:   NP_693541.1
- ProteinModelPortal:   Q8EN67
- GeneID:   1017085
- GenomeReviews:   BA000028_GR
- KEGG:   oih:OB2620
- NMPDR:   fig|221109.1.peg.2619
- HOGENOM:   HBG701974
- OMA:   DQAIISV
- ProtClustDB:   PRK00915
- BioCyc:   OIHE221109:OB2620-MONOMER
- BRENDA:   2.3.3.13
- HAMAP:   MF_01025_B
- InterPro:   IPR013709
- InterPro:   IPR002034
- InterPro:   IPR013785
- InterPro:   IPR005671
- InterPro:   IPR000891
- Gene3D:   G3DSA:3.20.20.70
- SMART:   SM00917
- TIGRFAMs:   TIGR00973

Pfam domain/function: PF00682 HMGL-like; PF08502 LeuA_dimer; SSF110921 2-isopropylmalate_synth_dimer

EC number: =2.3.3.13

Molecular weight: Translated: 56561; Mature: 56429

Theoretical pI: Translated: 4.98; Mature: 4.98

Prosite motif: PS00815 AIPM_HOMOCIT_SYNTH_1; PS00816 AIPM_HOMOCIT_SYNTH_2; PS50991 PYR_CT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRIKIFDTTLRDGEQSPGVNLNKAEKLEIAKQLEKYGVDRMEAGFPASSKGDFESVKQI
CCCEEEEECHHCCCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHCCCCCCCCCCHHHHHHH
AETIKSSSVIALARAVKSDIDIAYEALKGAEKPAIHIFLATSPIHMTYKLKKSPEQVIET
HHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCEEEEEEECCCHHHHHHH
AVNMVTYAKERFDEIEWSAEDATRSDWNFLAQIIEKVIEAGATVINLPDTVGYTTPEEYG
HHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEECCCCCCCCCHHHHH
KLFRFIKETVPNINQVALSCHCHNDLGMAVANSIAAVENGATQVEGTINGIGERAGNAAL
HHHHHHHHHCCCCEEEEEEEEECCCHHHHHHHHHHHHCCCCCEECHHHHHHHHHCCHHHH
EEVAVALKIRSDYYPFETGLILKETKRTSDLVAKLTGMYVQANKAVIGRNAFSHESGIHQ
HHHHHHEEECCCCCCCCCCEEEEHHHHHHHHHHHHHHHEEEECCEEEECCCCCCCCCCCC
DGVLKNTETYEIITPEMVGVSSNTLFLGKHSGRHAFKDKLKEFGVELSEEELKTAFEQFK
CCCCCCCCEEEEECCHHCCCCCCEEEEECCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHH
LLTDQKKEVTDDDLYTILMDIKTDSTVVDKYKLIQFGVSYDTAATPKANVILESPNGSLI
HHHCCCCCCCCCCEEEEEEECCCCCHHHHHEEEEEECCCCCCCCCCCCEEEEECCCCCEE
EATQQGNGSVEALYKTINYLIAENITLVDYQINSVGGGKDALAESHVQIIINGETINGRG
EECCCCCCHHHHHHHHHHHHHHCCEEEEEEEECCCCCCHHHHCCCCEEEEEECCEECCCC
SAQDVLQASAVAFIQAVNRYYVQKKANLTRLVYES
CHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECC
>Mature Secondary Structure 
SRIKIFDTTLRDGEQSPGVNLNKAEKLEIAKQLEKYGVDRMEAGFPASSKGDFESVKQI
CCEEEEECHHCCCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHCCCCCCCCCCHHHHHHH
AETIKSSSVIALARAVKSDIDIAYEALKGAEKPAIHIFLATSPIHMTYKLKKSPEQVIET
HHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCEEEEEEECCCHHHHHHH
AVNMVTYAKERFDEIEWSAEDATRSDWNFLAQIIEKVIEAGATVINLPDTVGYTTPEEYG
HHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEECCCCCCCCCHHHHH
KLFRFIKETVPNINQVALSCHCHNDLGMAVANSIAAVENGATQVEGTINGIGERAGNAAL
HHHHHHHHHCCCCEEEEEEEEECCCHHHHHHHHHHHHCCCCCEECHHHHHHHHHCCHHHH
EEVAVALKIRSDYYPFETGLILKETKRTSDLVAKLTGMYVQANKAVIGRNAFSHESGIHQ
HHHHHHEEECCCCCCCCCCEEEEHHHHHHHHHHHHHHHEEEECCEEEECCCCCCCCCCCC
DGVLKNTETYEIITPEMVGVSSNTLFLGKHSGRHAFKDKLKEFGVELSEEELKTAFEQFK
CCCCCCCCEEEEECCHHCCCCCCEEEEECCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHH
LLTDQKKEVTDDDLYTILMDIKTDSTVVDKYKLIQFGVSYDTAATPKANVILESPNGSLI
HHHCCCCCCCCCCEEEEEEECCCCCHHHHHEEEEEECCCCCCCCCCCCEEEEECCCCCEE
EATQQGNGSVEALYKTINYLIAENITLVDYQINSVGGGKDALAESHVQIIINGETINGRG
EECCCCCCHHHHHHHHHHHHHHCCEEEEEEEECCCCCCHHHHCCCCEEEEEECCEECCCC
SAQDVLQASAVAFIQAVNRYYVQKKANLTRLVYES
CHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12235376