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The map label for this gene is prfB [H]

Identifier: 23099950

GI number: 23099950

Start: 2553630

End: 2554613

Strand: Reverse

Name: prfB [H]

Synonym: OB2495

Alternate gene names: 23099950

Gene position: 2554613-2553630 (Counterclockwise)

Preceding gene: 23099951

Following gene: 23099949

Centisome position: 70.36

GC content: 37.09

Gene sequence:

>984_bases
ATGGCAGATCCTTCTTTTTGGGATGACCAAAATAAGGCTCAAAGTGTAATACAAGAGACAAATGGTATCAAAGAATATGT
TAATGGATTTGAAACAATAGAAGAGAATCTAGAAAACCTAGAAGTTTCTTATGAGTTAGTTAAAGAAGAAAATGACCAAG
AACTATTTGCTGATTTAGATGCTGAGCTTCAACAGTTTATAACAGATGTAAACCAATTTGAGTTACAGTTATTACTAAGT
GAACCGTATGATGCAAACAATGCTATTCTAGAACTGCACCCAGGCGCCGGAGGTACAGAATCTCAGGATTGGGCTAGCAT
GTTACTTAGAATGTATCAGCGCTGGGCAGAAGAACGTAATTTTAAAGTGGAAACGTTGGATTATTTGCCAGGTGATGAAG
CTGGTCTGAAAAGCGTCACGTTGATCATTAAAGGCCATAATGCCTATGGATATTTAAAAGCAGAAAAAGGAGTACATCGT
CTAGTACGAATCTCCCCATTTGATTCTTCTGGTAGAAGACACACTTCATTTGTTTCATGTGATGTTACACCGGAAATGTC
AGATGATGTCGATATTGATATTAGAACAGAGGATATTAAAGTAGATACGTATCGTGCAAGTGGTGCGGGTGGACAGCATG
TAAATACAACTGATTCTGCTGTTCGAATTACTCACCTACCTACAAACATCATTGTTACTTGTCAAAATGAACGTTCTCAA
ATAAAAAATAGAGAAGCGGCAATGAAGATGTTGAAATCCAAACTATATCAATTAGAAGTTGAAAAGCAAAGGCAAGAATT
AGATGATATACGCGGAGAACAAAAGGAAATTGGCTGGGGAAGTCAAATTAGATCGTATGTATTCCACCCATATTCTATGG
TGAAAGACCATCGAACAGACAAAGAGACTGGTAATACACAGGCGGTTATGGATGGAGATATAAATCCTTTCATTGATGCT
TATCTTCGTTCACAAATGGATTAA

Upstream 100 bases:

>100_bases
AGACAAGAATTGAATAAAATAGCTTCACGAGTTGAAGATTTTAGGGGGTCTCTTTGACTTAGATGTCAAAAAGGCTAGAA
TTGCTGAGTTAGAAGCAGAA

Downstream 100 bases:

>100_bases
GAATTTTTAAGTAAAAAACTGACGAAAAAATGGATATAACAAGTTCTTCCTAAGTTATCCCATCTTTTCATAAATTTGCC
ATAAACTTCTTCAATCCTAT

Product: peptide chain release factor RF-2

Products: NA

Alternate protein names: RF-2 [H]

Number of amino acids: Translated: 327; Mature: 326

Protein sequence:

>327_residues
MADPSFWDDQNKAQSVIQETNGIKEYVNGFETIEENLENLEVSYELVKEENDQELFADLDAELQQFITDVNQFELQLLLS
EPYDANNAILELHPGAGGTESQDWASMLLRMYQRWAEERNFKVETLDYLPGDEAGLKSVTLIIKGHNAYGYLKAEKGVHR
LVRISPFDSSGRRHTSFVSCDVTPEMSDDVDIDIRTEDIKVDTYRASGAGGQHVNTTDSAVRITHLPTNIIVTCQNERSQ
IKNREAAMKMLKSKLYQLEVEKQRQELDDIRGEQKEIGWGSQIRSYVFHPYSMVKDHRTDKETGNTQAVMDGDINPFIDA
YLRSQMD

Sequences:

>Translated_327_residues
MADPSFWDDQNKAQSVIQETNGIKEYVNGFETIEENLENLEVSYELVKEENDQELFADLDAELQQFITDVNQFELQLLLS
EPYDANNAILELHPGAGGTESQDWASMLLRMYQRWAEERNFKVETLDYLPGDEAGLKSVTLIIKGHNAYGYLKAEKGVHR
LVRISPFDSSGRRHTSFVSCDVTPEMSDDVDIDIRTEDIKVDTYRASGAGGQHVNTTDSAVRITHLPTNIIVTCQNERSQ
IKNREAAMKMLKSKLYQLEVEKQRQELDDIRGEQKEIGWGSQIRSYVFHPYSMVKDHRTDKETGNTQAVMDGDINPFIDA
YLRSQMD
>Mature_326_residues
ADPSFWDDQNKAQSVIQETNGIKEYVNGFETIEENLENLEVSYELVKEENDQELFADLDAELQQFITDVNQFELQLLLSE
PYDANNAILELHPGAGGTESQDWASMLLRMYQRWAEERNFKVETLDYLPGDEAGLKSVTLIIKGHNAYGYLKAEKGVHRL
VRISPFDSSGRRHTSFVSCDVTPEMSDDVDIDIRTEDIKVDTYRASGAGGQHVNTTDSAVRITHLPTNIIVTCQNERSQI
KNREAAMKMLKSKLYQLEVEKQRQELDDIRGEQKEIGWGSQIRSYVFHPYSMVKDHRTDKETGNTQAVMDGDINPFIDAY
LRSQMD

Specific function: Peptide chain release factor 2 directs the termination of translation in response to the peptide chain termination codons UGA and UAA [H]

COG id: COG1186

COG function: function code J; Protein chain release factor B

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family [H]

Homologues:

Organism=Homo sapiens, GI166795303, Length=254, Percent_Identity=42.1259842519685, Blast_Score=193, Evalue=1e-49,
Organism=Homo sapiens, GI34577120, Length=311, Percent_Identity=35.6913183279743, Blast_Score=172, Evalue=5e-43,
Organism=Homo sapiens, GI166795305, Length=167, Percent_Identity=43.7125748502994, Blast_Score=138, Evalue=6e-33,
Organism=Escherichia coli, GI2367172, Length=323, Percent_Identity=49.5356037151703, Blast_Score=328, Evalue=2e-91,
Organism=Escherichia coli, GI1787462, Length=245, Percent_Identity=41.6326530612245, Blast_Score=189, Evalue=1e-49,
Organism=Caenorhabditis elegans, GI17542784, Length=299, Percent_Identity=30.7692307692308, Blast_Score=110, Evalue=8e-25,
Organism=Saccharomyces cerevisiae, GI6321295, Length=299, Percent_Identity=34.1137123745819, Blast_Score=144, Evalue=2e-35,
Organism=Drosophila melanogaster, GI19921226, Length=260, Percent_Identity=39.6153846153846, Blast_Score=167, Evalue=7e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR020853
- InterPro:   IPR004374 [H]

Pfam domain/function: PF03462 PCRF; PF00472 RF-1 [H]

EC number: NA

Molecular weight: Translated: 37384; Mature: 37253

Theoretical pI: Translated: 4.41; Mature: 4.41

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADPSFWDDQNKAQSVIQETNGIKEYVNGFETIEENLENLEVSYELVKEENDQELFADLD
CCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
AELQQFITDVNQFELQLLLSEPYDANNAILELHPGAGGTESQDWASMLLRMYQRWAEERN
HHHHHHHHHHHHHEEEEEECCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCC
FKVETLDYLPGDEAGLKSVTLIIKGHNAYGYLKAEKGVHRLVRISPFDSSGRRHTSFVSC
CEEEEECCCCCCCCCCCEEEEEEECCCCEEEEECCCCCEEEEEECCCCCCCCCCCEEEEE
DVTPEMSDDVDIDIRTEDIKVDTYRASGAGGQHVNTTDSAVRITHLPTNIIVTCQNERSQ
ECCCCCCCCCEEEEEECEEEEEEEEECCCCCCCCCCCCCEEEEEECCCEEEEEECCHHHH
IKNREAAMKMLKSKLYQLEVEKQRQELDDIRGEQKEIGWGSQIRSYVFHPYSMVKDHRTD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCHHHHHHHHCCCHHHHHHCCCC
KETGNTQAVMDGDINPFIDAYLRSQMD
CCCCCCEEEECCCCCHHHHHHHHHCCC
>Mature Secondary Structure 
ADPSFWDDQNKAQSVIQETNGIKEYVNGFETIEENLENLEVSYELVKEENDQELFADLD
CCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
AELQQFITDVNQFELQLLLSEPYDANNAILELHPGAGGTESQDWASMLLRMYQRWAEERN
HHHHHHHHHHHHHEEEEEECCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCC
FKVETLDYLPGDEAGLKSVTLIIKGHNAYGYLKAEKGVHRLVRISPFDSSGRRHTSFVSC
CEEEEECCCCCCCCCCCEEEEEEECCCCEEEEECCCCCEEEEEECCCCCCCCCCCEEEEE
DVTPEMSDDVDIDIRTEDIKVDTYRASGAGGQHVNTTDSAVRITHLPTNIIVTCQNERSQ
ECCCCCCCCCEEEEEECEEEEEEEEECCCCCCCCCCCCCEEEEEECCCEEEEEECCHHHH
IKNREAAMKMLKSKLYQLEVEKQRQELDDIRGEQKEIGWGSQIRSYVFHPYSMVKDHRTD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCHHHHHHHHCCCHHHHHHCCCC
KETGNTQAVMDGDINPFIDAYLRSQMD
CCCCCCEEEECCCCCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9696765; 9384377; 1901557; 1408743 [H]