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The map label for this gene is cwlO [H]

Identifier: 23099946

GI number: 23099946

Start: 2549906

End: 2551288

Strand: Reverse

Name: cwlO [H]

Synonym: OB2491

Alternate gene names: 23099946

Gene position: 2551288-2549906 (Counterclockwise)

Preceding gene: 23099947

Following gene: 23099945

Centisome position: 70.27

GC content: 34.35

Gene sequence:

>1383_bases
TTGAAAAAGATAACTTCCATTCTAGTTATTACTTTATTAACATTCACTGTATTTGGACAAAATTTATTCGTTTCTGCTGA
ATCTGCAGGTGATGTTCAAAAACAAATTGAAGATCTTGAAAAAGAAAAATCGAATTTAGAAAAAGAAAGACAAGAAATTG
AGGACTCTAAGAAAGATACTGAGAGCAAAATGCAAGAAAATAAAAAAGAACAAAACTCAGTAGAGACTGAGATTAACAGT
ATTGATTCAGAATTAGAGGATACACAAAAGCAAATTGAAACGAAACAAACAGAAATTGATAATACAAATGATGAAATTAA
TAGTCTTACAGAAAAAGTTGAAGAATTAAAAGAACGGATGAAAGAACTAGAGGAAGAAATTAAACTTTTAAAAGAGCGGA
TTGAAAAGCGAGACGAATTATTGAAGAATCGCCTACTCTCTATTCAACAAAATGGTGGAGATATCAAATATTTAGAAGTT
ATTTTTGGTGCATCTAGCTTTGGAGACTTTATTTCTCGAAGTACCGCAGTAAACACCATTATGGACCAGGATAAAGAAAT
AATGGAAGAACAAGAAAGAGATAAACAGACTTTAGAAGAAAATAAAAAAGAAGTTGTAGCTAGTAAAGAGGAAGTTGAAG
AGAAGAAGCTTACTGTTGAAGAGAAGAAGAAATCTCTAGAAGGACAGCATGATGAGCTGCTCGCATTAAAAGATAAATTA
AATGATCAAGTTGCAGAAAAAGAAACACTGATGGCAGAGTTAGTGGATGAACACGAAGAGCTAGAAGAAATCAAACTTTC
AGCAGAAGAGGAACAACAACTTATTTCTGATGAAGCTGCTGCTAAAGAACAAGCGATAAAATTAGCTCAAGAGAAACAAG
CAAACCTTAAGAAAAAAGAGGAAGAAGAACAAAAAGCCTCTAACCAAGATAATAACTCAACAGCATCATCTGGAGGGGGT
AGTTCTGTTCCTAGTGGAGACTTCATTCGACCAACTACAGGACCAGTTACATCTGGATTTAATCCAGATCGCATGCATCC
TATTTTTAATGAAGTAAGACCACATACTGGTACAGATTTTGGAGCTTCTAACGCTGGAGATCGAAATATTATGGCTTCTG
CTGATGGAGTTGTTTATCAAGCTGGCGTTTTAAGTGGCTATGGTAATACAGTAATGATTACACATCACGTAAACGGACAA
ACCTTTACTACTCTATATGCACATTTAAACAGTATGTCAGTTTCAACAGGACAAACTGTATCACAGGGTGACAAAATAGG
AGTTATGGGTAATACTGGTAATTCAACAGGTGTACATCTGCATTTCGAAATTCACCCAGGTGGCTACAAGAATCCAGTAA
ATCCATTCAATTATATTAACTAG

Upstream 100 bases:

>100_bases
TTGGTGCTGTAATTGGTGTATGGGGAAGCGTAATGAGTGTACGTAAGTTCTTAAAAGTCTGATAGATGATGACGAATTCC
AAGCAGAGGGGGAAATACGA

Downstream 100 bases:

>100_bases
TATAATCAAATTTATAATTGCTATGTTTAAAAAATGAGCAAATAAGAAACCGAATTATAGATCGCAAATTACGATCTATA
ATTCGGTTTCTTGATTATAG

Product: hypothetical protein

Products: NA

Alternate protein names: D-gamma-glutamyl-meso-diaminopimelyl DL-endopeptidase; PSPA2 [H]

Number of amino acids: Translated: 460; Mature: 460

Protein sequence:

>460_residues
MKKITSILVITLLTFTVFGQNLFVSAESAGDVQKQIEDLEKEKSNLEKERQEIEDSKKDTESKMQENKKEQNSVETEINS
IDSELEDTQKQIETKQTEIDNTNDEINSLTEKVEELKERMKELEEEIKLLKERIEKRDELLKNRLLSIQQNGGDIKYLEV
IFGASSFGDFISRSTAVNTIMDQDKEIMEEQERDKQTLEENKKEVVASKEEVEEKKLTVEEKKKSLEGQHDELLALKDKL
NDQVAEKETLMAELVDEHEELEEIKLSAEEEQQLISDEAAAKEQAIKLAQEKQANLKKKEEEEQKASNQDNNSTASSGGG
SSVPSGDFIRPTTGPVTSGFNPDRMHPIFNEVRPHTGTDFGASNAGDRNIMASADGVVYQAGVLSGYGNTVMITHHVNGQ
TFTTLYAHLNSMSVSTGQTVSQGDKIGVMGNTGNSTGVHLHFEIHPGGYKNPVNPFNYIN

Sequences:

>Translated_460_residues
MKKITSILVITLLTFTVFGQNLFVSAESAGDVQKQIEDLEKEKSNLEKERQEIEDSKKDTESKMQENKKEQNSVETEINS
IDSELEDTQKQIETKQTEIDNTNDEINSLTEKVEELKERMKELEEEIKLLKERIEKRDELLKNRLLSIQQNGGDIKYLEV
IFGASSFGDFISRSTAVNTIMDQDKEIMEEQERDKQTLEENKKEVVASKEEVEEKKLTVEEKKKSLEGQHDELLALKDKL
NDQVAEKETLMAELVDEHEELEEIKLSAEEEQQLISDEAAAKEQAIKLAQEKQANLKKKEEEEQKASNQDNNSTASSGGG
SSVPSGDFIRPTTGPVTSGFNPDRMHPIFNEVRPHTGTDFGASNAGDRNIMASADGVVYQAGVLSGYGNTVMITHHVNGQ
TFTTLYAHLNSMSVSTGQTVSQGDKIGVMGNTGNSTGVHLHFEIHPGGYKNPVNPFNYIN
>Mature_460_residues
MKKITSILVITLLTFTVFGQNLFVSAESAGDVQKQIEDLEKEKSNLEKERQEIEDSKKDTESKMQENKKEQNSVETEINS
IDSELEDTQKQIETKQTEIDNTNDEINSLTEKVEELKERMKELEEEIKLLKERIEKRDELLKNRLLSIQQNGGDIKYLEV
IFGASSFGDFISRSTAVNTIMDQDKEIMEEQERDKQTLEENKKEVVASKEEVEEKKLTVEEKKKSLEGQHDELLALKDKL
NDQVAEKETLMAELVDEHEELEEIKLSAEEEQQLISDEAAAKEQAIKLAQEKQANLKKKEEEEQKASNQDNNSTASSGGG
SSVPSGDFIRPTTGPVTSGFNPDRMHPIFNEVRPHTGTDFGASNAGDRNIMASADGVVYQAGVLSGYGNTVMITHHVNGQ
TFTTLYAHLNSMSVSTGQTVSQGDKIGVMGNTGNSTGVHLHFEIHPGGYKNPVNPFNYIN

Specific function: The C-terminal part of CwlO shows a cell wall hydrolytic DL-endopeptidase activity [H]

COG id: COG3883

COG function: function code S; Uncharacterized protein conserved in bacteria

Gene ontology:

Cell location: Secreted. Secreted, cell wall [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase C40 family [H]

Homologues:

Organism=Escherichia coli, GI87081989, Length=147, Percent_Identity=36.0544217687075, Blast_Score=92, Evalue=7e-20,
Organism=Escherichia coli, GI1789099, Length=112, Percent_Identity=41.0714285714286, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI87082174, Length=185, Percent_Identity=28.6486486486486, Blast_Score=65, Evalue=8e-12,
Organism=Escherichia coli, GI87082297, Length=276, Percent_Identity=25, Blast_Score=62, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000064 [H]

Pfam domain/function: PF00877 NLPC_P60 [H]

EC number: 3.4.24.- [C]

Molecular weight: Translated: 51502; Mature: 51502

Theoretical pI: Translated: 4.45; Mature: 4.45

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKITSILVITLLTFTVFGQNLFVSAESAGDVQKQIEDLEKEKSNLEKERQEIEDSKKDT
CHHHHHHHHHHHHHHHHHCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ESKMQENKKEQNSVETEINSIDSELEDTQKQIETKQTEIDNTNDEINSLTEKVEELKERM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
KELEEEIKLLKERIEKRDELLKNRLLSIQQNGGDIKYLEVIFGASSFGDFISRSTAVNTI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHCCHHHHHHHHHHHHHHHH
MDQDKEIMEEQERDKQTLEENKKEVVASKEEVEEKKLTVEEKKKSLEGQHDELLALKDKL
HHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
NDQVAEKETLMAELVDEHEELEEIKLSAEEEQQLISDEAAAKEQAIKLAQEKQANLKKKE
HHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EEEQKASNQDNNSTASSGGGSSVPSGDFIRPTTGPVTSGFNPDRMHPIFNEVRPHTGTDF
HHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHCCHHHHHCCCCCCCCC
GASNAGDRNIMASADGVVYQAGVLSGYGNTVMITHHVNGQTFTTLYAHLNSMSVSTGQTV
CCCCCCCCCEEECCCCCEEEHHHHCCCCCEEEEEEECCCCHHHHHHHHHHHCCCCCCCCH
SQGDKIGVMGNTGNSTGVHLHFEIHPGGYKNPVNPFNYIN
HCCCEEEEEECCCCCCEEEEEEEECCCCCCCCCCCCCCCC
>Mature Secondary Structure
MKKITSILVITLLTFTVFGQNLFVSAESAGDVQKQIEDLEKEKSNLEKERQEIEDSKKDT
CHHHHHHHHHHHHHHHHHCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ESKMQENKKEQNSVETEINSIDSELEDTQKQIETKQTEIDNTNDEINSLTEKVEELKERM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
KELEEEIKLLKERIEKRDELLKNRLLSIQQNGGDIKYLEVIFGASSFGDFISRSTAVNTI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHCCHHHHHHHHHHHHHHHH
MDQDKEIMEEQERDKQTLEENKKEVVASKEEVEEKKLTVEEKKKSLEGQHDELLALKDKL
HHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
NDQVAEKETLMAELVDEHEELEEIKLSAEEEQQLISDEAAAKEQAIKLAQEKQANLKKKE
HHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EEEQKASNQDNNSTASSGGGSSVPSGDFIRPTTGPVTSGFNPDRMHPIFNEVRPHTGTDF
HHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHCCHHHHHCCCCCCCCC
GASNAGDRNIMASADGVVYQAGVLSGYGNTVMITHHVNGQTFTTLYAHLNSMSVSTGQTV
CCCCCCCCCEEECCCCCEEEHHHHCCCCCEEEEEEECCCCHHHHHHHHHHHCCCCCCCCH
SQGDKIGVMGNTGNSTGVHLHFEIHPGGYKNPVNPFNYIN
HCCCEEEEEECCCCCCEEEEEEEECCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377; 3145906 [H]