Click here to switch to the map view.

The map label for this gene is mutM

Identifier: 23099617

GI number: 23099617

Start: 2193996

End: 2194838

Strand: Reverse

Name: mutM

Synonym: OB2162

Alternate gene names: 23099617

Gene position: 2194838-2193996 (Counterclockwise)

Preceding gene: 23099618

Following gene: 23099616

Centisome position: 60.46

GC content: 35.59

Gene sequence:

>843_bases
ATGCCTGAATTACCTGAAGTAGAAACAATAAAAGAAACATTGAAACTTTTTGTATGCAATAAAACGATCAAGCATATTGA
TATTGAGTGGCCAAATATGATTAAGCATCCTGACGATGTTGAGGAATTTAAAGCATTAGTTACTGGTCAAACGATTCGTT
CAATGGGAAGAAAAGGAAAATTTCTTTTGTTTTATCTTGATGAATATGTTCTTATCTCCCATTTGCGGATGGAAGGAAAG
TATAGTGTTCATTCTCCAGGAGATCCAGTAAAAAAACATACCCATGTAACGTTCTATTTTTCAAATGGAGAAGAATTACG
TTATAACGATGTTCGGAAATTCGGAACCATGCATGTTTATCCAATTGGTGAAGAGTTTATGCATAAACCTTTAAACCAAC
TTGGTCCTGATCCGTTTGACACATCGTTTAATTTAGAATACTTTTATGAAAAATTGAAGCGAACAGATCGATATATTAAA
ACAGCTTTGTTAGATCAATCAATTGTTACTGGACTGGGGAATATTTATGTGGACGAGACCTTGTTTCGGGCGAATGTTCA
TCCGCTGAAACGTTGTAGCAAGTTATCGAAACAAGAAGTAAAAAAGTTACAAATAAATGCAAAGGAAACATTACGAGATG
CAATTAAAGCTGGAGGTACAACGATTCGTAGTTATGTGAATACCCAAGGAGATATGGGGATGTTTCAACAAGATTTATAT
GTTTATGGTCAGCATTCTAAGCCGTGTAGAGTTTGCGGCGCAGATATTATTAAAATAAAGGTTGGTGGAAGAGGCACTCA
TCTATGTCCTACTTGCCAGCCGAATAAGCAAGGTGTGAGATAA

Upstream 100 bases:

>100_bases
CGATGTAATGGAAAACACAGTGGAATTAGATGTACCTTTAAAGGTGGACTATTCATTTGGAAAAAGCTGGTTTGATGCTA
AGTAAGGAGAAAAAATAAAG

Downstream 100 bases:

>100_bases
TGACATTAGTTATTGGTTTAACAGGAGGAATAGCAAGTGGTAAAAGCACTGTCTCCTCCATGCTCCTTGAAAAAAACTTT
CCTGTAATTGATGCTGATCT

Product: formamidopyrimidine-DNA glycosylase

Products: NA

Alternate protein names: Fapy-DNA glycosylase; DNA-(apurinic or apyrimidinic site) lyase mutM; AP lyase mutM

Number of amino acids: Translated: 280; Mature: 279

Protein sequence:

>280_residues
MPELPEVETIKETLKLFVCNKTIKHIDIEWPNMIKHPDDVEEFKALVTGQTIRSMGRKGKFLLFYLDEYVLISHLRMEGK
YSVHSPGDPVKKHTHVTFYFSNGEELRYNDVRKFGTMHVYPIGEEFMHKPLNQLGPDPFDTSFNLEYFYEKLKRTDRYIK
TALLDQSIVTGLGNIYVDETLFRANVHPLKRCSKLSKQEVKKLQINAKETLRDAIKAGGTTIRSYVNTQGDMGMFQQDLY
VYGQHSKPCRVCGADIIKIKVGGRGTHLCPTCQPNKQGVR

Sequences:

>Translated_280_residues
MPELPEVETIKETLKLFVCNKTIKHIDIEWPNMIKHPDDVEEFKALVTGQTIRSMGRKGKFLLFYLDEYVLISHLRMEGK
YSVHSPGDPVKKHTHVTFYFSNGEELRYNDVRKFGTMHVYPIGEEFMHKPLNQLGPDPFDTSFNLEYFYEKLKRTDRYIK
TALLDQSIVTGLGNIYVDETLFRANVHPLKRCSKLSKQEVKKLQINAKETLRDAIKAGGTTIRSYVNTQGDMGMFQQDLY
VYGQHSKPCRVCGADIIKIKVGGRGTHLCPTCQPNKQGVR
>Mature_279_residues
PELPEVETIKETLKLFVCNKTIKHIDIEWPNMIKHPDDVEEFKALVTGQTIRSMGRKGKFLLFYLDEYVLISHLRMEGKY
SVHSPGDPVKKHTHVTFYFSNGEELRYNDVRKFGTMHVYPIGEEFMHKPLNQLGPDPFDTSFNLEYFYEKLKRTDRYIKT
ALLDQSIVTGLGNIYVDETLFRANVHPLKRCSKLSKQEVKKLQINAKETLRDAIKAGGTTIRSYVNTQGDMGMFQQDLYV
YGQHSKPCRVCGADIIKIKVGGRGTHLCPTCQPNKQGVR

Specific function: Involved in base excision repair of DNA damaged by oxidation or by mutagenic agents. Acts as DNA glycosylase that recognizes and removes damaged bases. Has a preference for oxidized purines, such as 7,8-dihydro-8-oxoguanine (8-oxoG). Has AP (apurinic/apyr

COG id: COG0266

COG function: function code L; Formamidopyrimidine-DNA glycosylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FPG-type zinc finger

Homologues:

Organism=Escherichia coli, GI1790066, Length=273, Percent_Identity=37.7289377289377, Blast_Score=183, Evalue=1e-47,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): FPG_OCEIH (Q8EPE6)

Other databases:

- EMBL:   BA000028
- RefSeq:   NP_693083.1
- ProteinModelPortal:   Q8EPE6
- SMR:   Q8EPE6
- GeneID:   1015604
- GenomeReviews:   BA000028_GR
- KEGG:   oih:OB2162
- NMPDR:   fig|221109.1.peg.2160
- HOGENOM:   HBG690070
- OMA:   RMTGQLL
- ProtClustDB:   PRK01103
- BioCyc:   OIHE221109:OB2162-MONOMER
- BRENDA:   3.2.2.23
- BRENDA:   4.2.99.18
- HAMAP:   MF_00103
- InterPro:   IPR015886
- InterPro:   IPR015887
- InterPro:   IPR000191
- InterPro:   IPR012319
- InterPro:   IPR020629
- InterPro:   IPR010979
- InterPro:   IPR000214
- InterPro:   IPR010663
- SMART:   SM00898
- TIGRFAMs:   TIGR00577

Pfam domain/function: PF01149 Fapy_DNA_glyco; PF06831 H2TH; PF06827 zf-FPG_IleRS; SSF81624 Form_DNAglyc_cat; SSF46946 Ribosomal_H2TH

EC number: =3.2.2.23; =4.2.99.18

Molecular weight: Translated: 32236; Mature: 32104

Theoretical pI: Translated: 9.21; Mature: 9.21

Prosite motif: PS51068 FPG_CAT; PS01242 ZF_FPG_1; PS51066 ZF_FPG_2

Important sites: ACT_SITE 2-2 ACT_SITE 3-3 ACT_SITE 60-60 ACT_SITE 264-264 BINDING 93-93 BINDING 112-112

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPELPEVETIKETLKLFVCNKTIKHIDIEWPNMIKHPDDVEEFKALVTGQTIRSMGRKGK
CCCCCCHHHHHHHHHHHHHCCHHHEEECCCCHHCCCCCCHHHHHHHHHHHHHHHHCCCCC
FLLFYLDEYVLISHLRMEGKYSVHSPGDPVKKHTHVTFYFSNGEELRYNDVRKFGTMHVY
EEEEEHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEECCCCEECHHHHHHHCCEEEE
PIGEEFMHKPLNQLGPDPFDTSFNLEYFYEKLKRTDRYIKTALLDQSIVTGLGNIYVDET
ECCHHHHHCHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LFRANVHPLKRCSKLSKQEVKKLQINAKETLRDAIKAGGTTIRSYVNTQGDMGMFQQDLY
HHHCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCHHHHHHHCCCCCCHHHHHHHE
VYGQHSKPCRVCGADIIKIKVGGRGTHLCPTCQPNKQGVR
EECCCCCCHHCCCCEEEEEEECCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
PELPEVETIKETLKLFVCNKTIKHIDIEWPNMIKHPDDVEEFKALVTGQTIRSMGRKGK
CCCCCHHHHHHHHHHHHHCCHHHEEECCCCHHCCCCCCHHHHHHHHHHHHHHHHCCCCC
FLLFYLDEYVLISHLRMEGKYSVHSPGDPVKKHTHVTFYFSNGEELRYNDVRKFGTMHVY
EEEEEHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEECCCCEECHHHHHHHCCEEEE
PIGEEFMHKPLNQLGPDPFDTSFNLEYFYEKLKRTDRYIKTALLDQSIVTGLGNIYVDET
ECCHHHHHCHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LFRANVHPLKRCSKLSKQEVKKLQINAKETLRDAIKAGGTTIRSYVNTQGDMGMFQQDLY
HHHCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCHHHHHHHCCCCCCHHHHHHHE
VYGQHSKPCRVCGADIIKIKVGGRGTHLCPTCQPNKQGVR
EECCCCCCHHCCCCEEEEEEECCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12235376