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The map label for this gene is polA [H]

Identifier: 23099618

GI number: 23099618

Start: 2194854

End: 2197496

Strand: Reverse

Name: polA [H]

Synonym: OB2163

Alternate gene names: 23099618

Gene position: 2197496-2194854 (Counterclockwise)

Preceding gene: 23099619

Following gene: 23099617

Centisome position: 60.53

GC content: 34.58

Gene sequence:

>2643_bases
ATGTCAAATAAGTTAGTGCTCATTGATGGAAATAGTATTATCTATCGAGCATTTTTTGCGCTGCCTTTGTTAAATAATGA
TAAAGGTGTTTATACCAATGGAGTATATGGATTTACTACAATGCTATTGCGTATTTTAGAAGAAGAGAAGCCAACACATA
TGTTAGTAGCTTTTGATGCAGGTAAGACAACATTTCGTCATTCAACTTATAAAGAATATAAAGGTGGACGTCAAAAAACC
CCACCTGAGCTTTCGGAACAATTTCCCGTTCTCAAAGAGTTACTGGATGCTTTCTCTATTAAATATTATCAACTTGATCA
ATATGAAGCGGACGATATTATTGGTACGTTATCCAAACAAGCAGATAAAAAAGATTGGGAAGTTACCGTTATTTCAGGTG
ATAAAGATTTATTGCAACTGGTCTCTGAACAAGTAACGGTAAGTGTCACAAAAAAAGGTATTAGCGATATTGAAAAATAT
ACTCCAGCCTATATGTTGGAAAAAATGGAAATAACTCCAGACCAGATTATCCATTTAAAAGCATTAATGGGTGATAGCTC
AGATAACATTCCAGGCGTTCCAGGAGTAGGTGAGAAAACTGCTACAAAATTACTTAAACAATATGAGAACCTTGAAAACG
TTTATGAGCATATAGATGAAATATCTGGAAAGAAATTAAAAGAGAATTTATCTAATCATCGAGAAGATGCCTTTATGAGT
AAGGAGTTAGTAACGATAAATCGTGAATCACCTATTGAGATAACTCTTGAAAACTTACCTTATCAAGGTTTTCAAACCTC
GAGTGTTGTACAAATATTTAAGGATCTTGGTTTCCAGTCACTACTATCCAGAGTTAGTGAAGAGACAGAAGATGACGCTG
AGCAACCTAATATAGAGATGGAGTCCATTGAATTTGATGTGGTGGAGAATGTAACGAAGGATTTATTTACGGATAATGAT
GCGTTGTACATTGAAATGCTTGGAGAAAATTATCATCTAATTCCAATTGAAGGAATAGCTGTTGTCAATAAAGAGAGGGC
TTCTTTTATCCCAACAGATATTGCACTCAATTCTAATATATTTAAAGCATGGGCGGAGGACCCACAAAAACATAAAATTG
TTTTTGATGCAAAGCGAACTAAGGTTGCCTTGCTTCGTAACAATATAAATATGCAAGGAATTATTTTTGATTCCCTTCTG
GCATCTTATCTATTAAATCCGGCTGAGAACAATCATGATATACCAGCGATTGCAAATCGGAAAAGTAGATCGGATGTGCA
ATTTGACGAAGAAGTGTTTGGAAAAGGCGCAAAACAAAAAGTTCCTGAAGCAGACGTTTTGAATGAGCATGTTGTTCGAA
AAACACAATTATTATTTGATCTCTATCCCGAAATGGAAGAAGAACTAGGAAATAATGAACAATATGAGTTATTAAAAGAA
TTAGAGATGCCATTAGCTCTTATCTTAGCTGAGGTGGAACATTATGGTGTTCAAGTAGATATTGATAAGTTAAAAGATAT
GGGTGAGGATCTAAAAGAAAGGCTAAGTACACTGGAGCAAGAAGTTTATGAATTAGCCGGAGAGACATTTAATTTAAATT
CTCCAAAACAGTTGGGGCCAATCTTGTTTGAAAAATTAGGATTACCTGTTATTAAAAAAACAAAGACTGGTTATTCTACT
GCTGCAGATGTGTTAGAACAACTCGAAAATGAACATGATATAATCCCAAAACTATTAATGTATCGTCAATTAGGAAAGTT
GCAATCCACTTACATTGAGGGGTTATTAAAGGTTGTGCGGAAAGATACAAGCCGAATTCATACAAGATATAACCAAGCTT
TAACACAAACAGGTAGGTTGAGTTCGATTGAACCAAACTTACAAAATATACCTATCCGTTTAGAAGAAGGTAGAAAAATC
AGACAAGCTTTCGTACCATCGAAAGATAATTGGATTATGTTTGCTGCAGACTACTCTCAAATTGAGCTTCGTGTATTAGC
GCATATTGCAGGAGACGAAAAGTTAATTAAAGCATTTAAAGAAGATACCGATATTCATACCCAAACAGCTATGGATGTGT
TTCATGTAGAGTCGGATGAAGTAACGTCAAATATGCGAAGACAAGCCAAGGCAGTAAACTTTGGTATTGTTTATGGGATA
AGTGATTATGGTTTATCACAAAACCTTGGTATAACGCGCAAGGAAGCGAAAAAATTTATCGAGAGATATTTCAATAGTTA
TCCTGGTGTAAAAACGTACATGGATGAAATCGTGCAAGAAGCTAAGCATAAGGGATTTGTATCAACAATCATGAAACGGA
GAAGATACTTACCCGATATTACAAGTAGAAACTTTAATATGCGTAGTTTTGCAGAACGTACAGCAATGAACACTCCAATT
CAAGGAAGTGCTGCAGATATTATTAAAAAGGCTATGATTGATCTTGATCATCGTTTGAAAGAAGAAAATTTACAAGCAAA
TGTATTACTACAAGTACACGATGAGCTTATTTTAGAAGCACCAGAAAATGAAATTGATAAATTAAAGGAAATAGTACCCG
ATGTAATGGAAAACACAGTGGAATTAGATGTACCTTTAAAGGTGGACTATTCATTTGGAAAAAGCTGGTTTGATGCTAAG
TAA

Upstream 100 bases:

>100_bases
CGTTAGGTAATAAATGCATGAAATTCACGTATTTTTTCCTTTTCTTGAATGAGAATGCTAAAATAGAGCTAAGGATTTAG
ATGAGAGGATGGTAAATCGA

Downstream 100 bases:

>100_bases
GGAGAAAAAATAAAGATGCCTGAATTACCTGAAGTAGAAACAATAAAAGAAACATTGAAACTTTTTGTATGCAATAAAAC
GATCAAGCATATTGATATTG

Product: DNA polymerase I

Products: NA

Alternate protein names: POL I [H]

Number of amino acids: Translated: 880; Mature: 879

Protein sequence:

>880_residues
MSNKLVLIDGNSIIYRAFFALPLLNNDKGVYTNGVYGFTTMLLRILEEEKPTHMLVAFDAGKTTFRHSTYKEYKGGRQKT
PPELSEQFPVLKELLDAFSIKYYQLDQYEADDIIGTLSKQADKKDWEVTVISGDKDLLQLVSEQVTVSVTKKGISDIEKY
TPAYMLEKMEITPDQIIHLKALMGDSSDNIPGVPGVGEKTATKLLKQYENLENVYEHIDEISGKKLKENLSNHREDAFMS
KELVTINRESPIEITLENLPYQGFQTSSVVQIFKDLGFQSLLSRVSEETEDDAEQPNIEMESIEFDVVENVTKDLFTDND
ALYIEMLGENYHLIPIEGIAVVNKERASFIPTDIALNSNIFKAWAEDPQKHKIVFDAKRTKVALLRNNINMQGIIFDSLL
ASYLLNPAENNHDIPAIANRKSRSDVQFDEEVFGKGAKQKVPEADVLNEHVVRKTQLLFDLYPEMEEELGNNEQYELLKE
LEMPLALILAEVEHYGVQVDIDKLKDMGEDLKERLSTLEQEVYELAGETFNLNSPKQLGPILFEKLGLPVIKKTKTGYST
AADVLEQLENEHDIIPKLLMYRQLGKLQSTYIEGLLKVVRKDTSRIHTRYNQALTQTGRLSSIEPNLQNIPIRLEEGRKI
RQAFVPSKDNWIMFAADYSQIELRVLAHIAGDEKLIKAFKEDTDIHTQTAMDVFHVESDEVTSNMRRQAKAVNFGIVYGI
SDYGLSQNLGITRKEAKKFIERYFNSYPGVKTYMDEIVQEAKHKGFVSTIMKRRRYLPDITSRNFNMRSFAERTAMNTPI
QGSAADIIKKAMIDLDHRLKEENLQANVLLQVHDELILEAPENEIDKLKEIVPDVMENTVELDVPLKVDYSFGKSWFDAK

Sequences:

>Translated_880_residues
MSNKLVLIDGNSIIYRAFFALPLLNNDKGVYTNGVYGFTTMLLRILEEEKPTHMLVAFDAGKTTFRHSTYKEYKGGRQKT
PPELSEQFPVLKELLDAFSIKYYQLDQYEADDIIGTLSKQADKKDWEVTVISGDKDLLQLVSEQVTVSVTKKGISDIEKY
TPAYMLEKMEITPDQIIHLKALMGDSSDNIPGVPGVGEKTATKLLKQYENLENVYEHIDEISGKKLKENLSNHREDAFMS
KELVTINRESPIEITLENLPYQGFQTSSVVQIFKDLGFQSLLSRVSEETEDDAEQPNIEMESIEFDVVENVTKDLFTDND
ALYIEMLGENYHLIPIEGIAVVNKERASFIPTDIALNSNIFKAWAEDPQKHKIVFDAKRTKVALLRNNINMQGIIFDSLL
ASYLLNPAENNHDIPAIANRKSRSDVQFDEEVFGKGAKQKVPEADVLNEHVVRKTQLLFDLYPEMEEELGNNEQYELLKE
LEMPLALILAEVEHYGVQVDIDKLKDMGEDLKERLSTLEQEVYELAGETFNLNSPKQLGPILFEKLGLPVIKKTKTGYST
AADVLEQLENEHDIIPKLLMYRQLGKLQSTYIEGLLKVVRKDTSRIHTRYNQALTQTGRLSSIEPNLQNIPIRLEEGRKI
RQAFVPSKDNWIMFAADYSQIELRVLAHIAGDEKLIKAFKEDTDIHTQTAMDVFHVESDEVTSNMRRQAKAVNFGIVYGI
SDYGLSQNLGITRKEAKKFIERYFNSYPGVKTYMDEIVQEAKHKGFVSTIMKRRRYLPDITSRNFNMRSFAERTAMNTPI
QGSAADIIKKAMIDLDHRLKEENLQANVLLQVHDELILEAPENEIDKLKEIVPDVMENTVELDVPLKVDYSFGKSWFDAK
>Mature_879_residues
SNKLVLIDGNSIIYRAFFALPLLNNDKGVYTNGVYGFTTMLLRILEEEKPTHMLVAFDAGKTTFRHSTYKEYKGGRQKTP
PELSEQFPVLKELLDAFSIKYYQLDQYEADDIIGTLSKQADKKDWEVTVISGDKDLLQLVSEQVTVSVTKKGISDIEKYT
PAYMLEKMEITPDQIIHLKALMGDSSDNIPGVPGVGEKTATKLLKQYENLENVYEHIDEISGKKLKENLSNHREDAFMSK
ELVTINRESPIEITLENLPYQGFQTSSVVQIFKDLGFQSLLSRVSEETEDDAEQPNIEMESIEFDVVENVTKDLFTDNDA
LYIEMLGENYHLIPIEGIAVVNKERASFIPTDIALNSNIFKAWAEDPQKHKIVFDAKRTKVALLRNNINMQGIIFDSLLA
SYLLNPAENNHDIPAIANRKSRSDVQFDEEVFGKGAKQKVPEADVLNEHVVRKTQLLFDLYPEMEEELGNNEQYELLKEL
EMPLALILAEVEHYGVQVDIDKLKDMGEDLKERLSTLEQEVYELAGETFNLNSPKQLGPILFEKLGLPVIKKTKTGYSTA
ADVLEQLENEHDIIPKLLMYRQLGKLQSTYIEGLLKVVRKDTSRIHTRYNQALTQTGRLSSIEPNLQNIPIRLEEGRKIR
QAFVPSKDNWIMFAADYSQIELRVLAHIAGDEKLIKAFKEDTDIHTQTAMDVFHVESDEVTSNMRRQAKAVNFGIVYGIS
DYGLSQNLGITRKEAKKFIERYFNSYPGVKTYMDEIVQEAKHKGFVSTIMKRRRYLPDITSRNFNMRSFAERTAMNTPIQ
GSAADIIKKAMIDLDHRLKEENLQANVLLQVHDELILEAPENEIDKLKEIVPDVMENTVELDVPLKVDYSFGKSWFDAK

Specific function: In addition to polymerase activity, this DNA polymerase exhibits 3' to 5' and 5' to 3' exonuclease activity [H]

COG id: COG0749

COG function: function code L; DNA polymerase I - 3'-5' exonuclease and polymerase domains

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 5'-3' exonuclease domain [H]

Homologues:

Organism=Homo sapiens, GI112420958, Length=517, Percent_Identity=32.1083172147002, Blast_Score=251, Evalue=2e-66,
Organism=Homo sapiens, GI139394648, Length=649, Percent_Identity=25.8859784283513, Blast_Score=211, Evalue=2e-54,
Organism=Escherichia coli, GI1790294, Length=928, Percent_Identity=37.823275862069, Blast_Score=575, Evalue=1e-165,
Organism=Escherichia coli, GI226510970, Length=221, Percent_Identity=28.0542986425339, Blast_Score=109, Evalue=6e-25,
Organism=Caenorhabditis elegans, GI86563272, Length=410, Percent_Identity=31.219512195122, Blast_Score=201, Evalue=1e-51,
Organism=Drosophila melanogaster, GI17933644, Length=426, Percent_Identity=33.0985915492958, Blast_Score=240, Evalue=3e-63,

Paralogues:

None

Copy number: 400 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002562
- InterPro:   IPR020046
- InterPro:   IPR020045
- InterPro:   IPR002421
- InterPro:   IPR020047
- InterPro:   IPR019760
- InterPro:   IPR001098
- InterPro:   IPR018320
- InterPro:   IPR002298
- InterPro:   IPR008918
- InterPro:   IPR003583
- InterPro:   IPR012337 [H]

Pfam domain/function: PF01367 5_3_exonuc; PF02739 5_3_exonuc_N; PF00476 DNA_pol_A [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 100499; Mature: 100368

Theoretical pI: Translated: 4.81; Mature: 4.81

Prosite motif: PS00447 DNA_POLYMERASE_A

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNKLVLIDGNSIIYRAFFALPLLNNDKGVYTNGVYGFTTMLLRILEEEKPTHMLVAFDA
CCCCEEEEECCHHHHHHHHHHHEECCCCCCEECCHHHHHHHHHHHHHCCCCCEEEEEEEC
GKTTFRHSTYKEYKGGRQKTPPELSEQFPVLKELLDAFSIKYYQLDQYEADDIIGTLSKQ
CCCHHHHCHHHHHCCCCCCCCCCHHHCCHHHHHHHHHHCCEEEEECCCCHHHHHHHHHHH
ADKKDWEVTVISGDKDLLQLVSEQVTVSVTKKGISDIEKYTPAYMLEKMEITPDQIIHLK
CCCCCCEEEEEECCHHHHHHHHHHHEEHHHHHHHHHHHHHCHHHHHHHCCCCHHHHHHHH
ALMGDSSDNIPGVPGVGEKTATKLLKQYENLENVYEHIDEISGKKLKENLSNHREDAFMS
HHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHH
KELVTINRESPIEITLENLPYQGFQTSSVVQIFKDLGFQSLLSRVSEETEDDAEQPNIEM
HHHEEECCCCCCEEEECCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHCCCCHHCCCCCCH
ESIEFDVVENVTKDLFTDNDALYIEMLGENYHLIPIEGIAVVNKERASFIPTDIALNSNI
HHHHHHHHHHHHHHHHCCCCEEEEEEECCCEEEEEECCEEEECCCCCCCCCCHHEECCHH
FKAWAEDPQKHKIVFDAKRTKVALLRNNINMQGIIFDSLLASYLLNPAENNHDIPAIANR
HHHHHCCCCCCEEEEECCHHEEEHHHCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCC
KSRSDVQFDEEVFGKGAKQKVPEADVLNEHVVRKTQLLFDLYPEMEEELGNNEQYELLKE
CCCCCCCCCHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
LEMPLALILAEVEHYGVQVDIDKLKDMGEDLKERLSTLEQEVYELAGETFNLNSPKQLGP
HCCHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHH
ILFEKLGLPVIKKTKTGYSTAADVLEQLENEHDIIPKLLMYRQLGKLQSTYIEGLLKVVR
HHHHHHCCCEEEECCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KDTSRIHTRYNQALTQTGRLSSIEPNLQNIPIRLEEGRKIRQAFVPSKDNWIMFAADYSQ
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEECCCHHHHHHHCCCCCCEEEEEECCCH
IELRVLAHIAGDEKLIKAFKEDTDIHTQTAMDVFHVESDEVTSNMRRQAKAVNFGIVYGI
HHHHHHHHHCCCHHHHHHHHHCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHCEEEEEEE
SDYGLSQNLGITRKEAKKFIERYFNSYPGVKTYMDEIVQEAKHKGFVSTIMKRRRYLPDI
CCCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCC
TSRNFNMRSFAERTAMNTPIQGSAADIIKKAMIDLDHRLKEENLQANVLLQVHDELILEA
CCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEHEEEHHHHHHCC
PENEIDKLKEIVPDVMENTVELDVPLKVDYSFGKSWFDAK
CCHHHHHHHHHHHHHHCCCEEEECEEEEEHHHCCCCCCCC
>Mature Secondary Structure 
SNKLVLIDGNSIIYRAFFALPLLNNDKGVYTNGVYGFTTMLLRILEEEKPTHMLVAFDA
CCCEEEEECCHHHHHHHHHHHEECCCCCCEECCHHHHHHHHHHHHHCCCCCEEEEEEEC
GKTTFRHSTYKEYKGGRQKTPPELSEQFPVLKELLDAFSIKYYQLDQYEADDIIGTLSKQ
CCCHHHHCHHHHHCCCCCCCCCCHHHCCHHHHHHHHHHCCEEEEECCCCHHHHHHHHHHH
ADKKDWEVTVISGDKDLLQLVSEQVTVSVTKKGISDIEKYTPAYMLEKMEITPDQIIHLK
CCCCCCEEEEEECCHHHHHHHHHHHEEHHHHHHHHHHHHHCHHHHHHHCCCCHHHHHHHH
ALMGDSSDNIPGVPGVGEKTATKLLKQYENLENVYEHIDEISGKKLKENLSNHREDAFMS
HHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHH
KELVTINRESPIEITLENLPYQGFQTSSVVQIFKDLGFQSLLSRVSEETEDDAEQPNIEM
HHHEEECCCCCCEEEECCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHCCCCHHCCCCCCH
ESIEFDVVENVTKDLFTDNDALYIEMLGENYHLIPIEGIAVVNKERASFIPTDIALNSNI
HHHHHHHHHHHHHHHHCCCCEEEEEEECCCEEEEEECCEEEECCCCCCCCCCHHEECCHH
FKAWAEDPQKHKIVFDAKRTKVALLRNNINMQGIIFDSLLASYLLNPAENNHDIPAIANR
HHHHHCCCCCCEEEEECCHHEEEHHHCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCC
KSRSDVQFDEEVFGKGAKQKVPEADVLNEHVVRKTQLLFDLYPEMEEELGNNEQYELLKE
CCCCCCCCCHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
LEMPLALILAEVEHYGVQVDIDKLKDMGEDLKERLSTLEQEVYELAGETFNLNSPKQLGP
HCCHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHH
ILFEKLGLPVIKKTKTGYSTAADVLEQLENEHDIIPKLLMYRQLGKLQSTYIEGLLKVVR
HHHHHHCCCEEEECCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KDTSRIHTRYNQALTQTGRLSSIEPNLQNIPIRLEEGRKIRQAFVPSKDNWIMFAADYSQ
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEECCCHHHHHHHCCCCCCEEEEEECCCH
IELRVLAHIAGDEKLIKAFKEDTDIHTQTAMDVFHVESDEVTSNMRRQAKAVNFGIVYGI
HHHHHHHHHCCCHHHHHHHHHCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHCEEEEEEE
SDYGLSQNLGITRKEAKKFIERYFNSYPGVKTYMDEIVQEAKHKGFVSTIMKRRRYLPDI
CCCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCC
TSRNFNMRSFAERTAMNTPIQGSAADIIKKAMIDLDHRLKEENLQANVLLQVHDELILEA
CCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEHEEEHHHHHHCC
PENEIDKLKEIVPDVMENTVELDVPLKVDYSFGKSWFDAK
CCHHHHHHHHHHHHHHCCCEEEECEEEEEHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9387221; 9384377 [H]