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The map label for this gene is cysH

Identifier: 23099107

GI number: 23099107

Start: 1694572

End: 1695294

Strand: Direct

Name: cysH

Synonym: OB1652

Alternate gene names: 23099107

Gene position: 1694572-1695294 (Clockwise)

Preceding gene: 23099106

Following gene: 23099108

Centisome position: 46.68

GC content: 39.7

Gene sequence:

>723_bases
ATGGGTGGCTATTCAGTTACCTATAGTAATTTTGTTAAGGATCCATATAAAGACTGGTTTCCTGACGATGAGACGAATGG
TGCTTCAAAAATATTGCAATGGGCTTATGAAGTATATGGAACGGATATCGTATATGCTTGCAGCTTTGGAGCGGAAGGAA
TGGTGCTGATCGATCTCATTTCGAAGACACAAAAGAATGCTGATATTGTCTTTCTTGATACTGATCTTCATTTTCAGGAA
ACATATGACCTGATAGAAGATATAAAAGTGAAATACCCAACACTCAACATTCATATCAAGAAGCCTGATTTAACGTTGGA
AGAGCAGGCAGCCGAACATGGTTCAGCTTTATGGAAGCGTCAGCCAGACCAATGCTGTTATATTCGTAAAATTAAGCCAT
TAGAGGATGCATTAAGTGGTGCACCAGCATGGATATCTGGTCTACGGAGAGAACAGTCCGTATCAAGAAGCAAAACAGAT
TTTATAAACAAAGATGAACGGTTTAAATCTATCAAAGTTTGTCCCTTAATTCATTGGACGTGGGACGATGTATGGGAGTA
TATCAAAGACAATAACTTACCGTACAATGAATTACATGATAATGGATATCCGAGTATTGGGTGTATTCCTTGTACTTCCC
AAGTGAGTGATTCCTCTGATTCACGTGCAGGAAGATGGGCGGGTACCCAAAAGACGGAATGTGGTCTCCATACTTCCGAT
TAA

Upstream 100 bases:

>100_bases
TACAATTCACATTGTATTACTATGAATTATATTCATAAGAGAAGACGCGATTAAACCGAATTCAATCTGCATGTATTATA
ACGTCTAGGAGGGATGTTAA

Downstream 100 bases:

>100_bases
TTACTGGAAAGTTCTTTATTTTTAAGAAGATGCCTTTTTTATAAAATCCGAGTAAATTGATATGTAAACTTATGAAATTA
AAAATTACTTGATGTATCTA

Product: 3'-phosphoadenosine 5'-phosphosulfate reductase

Products: NA

Alternate protein names: 3'-phosphoadenylylsulfate reductase; PAPS reductase, thioredoxin dependent; PAPS sulfotransferase; PAdoPS reductase

Number of amino acids: Translated: 240; Mature: 239

Protein sequence:

>240_residues
MGGYSVTYSNFVKDPYKDWFPDDETNGASKILQWAYEVYGTDIVYACSFGAEGMVLIDLISKTQKNADIVFLDTDLHFQE
TYDLIEDIKVKYPTLNIHIKKPDLTLEEQAAEHGSALWKRQPDQCCYIRKIKPLEDALSGAPAWISGLRREQSVSRSKTD
FINKDERFKSIKVCPLIHWTWDDVWEYIKDNNLPYNELHDNGYPSIGCIPCTSQVSDSSDSRAGRWAGTQKTECGLHTSD

Sequences:

>Translated_240_residues
MGGYSVTYSNFVKDPYKDWFPDDETNGASKILQWAYEVYGTDIVYACSFGAEGMVLIDLISKTQKNADIVFLDTDLHFQE
TYDLIEDIKVKYPTLNIHIKKPDLTLEEQAAEHGSALWKRQPDQCCYIRKIKPLEDALSGAPAWISGLRREQSVSRSKTD
FINKDERFKSIKVCPLIHWTWDDVWEYIKDNNLPYNELHDNGYPSIGCIPCTSQVSDSSDSRAGRWAGTQKTECGLHTSD
>Mature_239_residues
GGYSVTYSNFVKDPYKDWFPDDETNGASKILQWAYEVYGTDIVYACSFGAEGMVLIDLISKTQKNADIVFLDTDLHFQET
YDLIEDIKVKYPTLNIHIKKPDLTLEEQAAEHGSALWKRQPDQCCYIRKIKPLEDALSGAPAWISGLRREQSVSRSKTDF
INKDERFKSIKVCPLIHWTWDDVWEYIKDNNLPYNELHDNGYPSIGCIPCTSQVSDSSDSRAGRWAGTQKTECGLHTSD

Specific function: Reduction of activated sulfate into sulfite

COG id: COG0175

COG function: function code EH; 3'-phosphoadenosine 5'-phosphosulfate sulfotransferase (PAPS reductase)/FAD synthetase and related enzymes

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PAPS reductase family. CysH subfamily

Homologues:

Organism=Escherichia coli, GI1789121, Length=209, Percent_Identity=29.6650717703349, Blast_Score=89, Evalue=3e-19,
Organism=Saccharomyces cerevisiae, GI6325425, Length=221, Percent_Identity=36.6515837104072, Blast_Score=137, Evalue=1e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): CYSH_OCEIH (Q8EQP2)

Other databases:

- EMBL:   BA000028
- RefSeq:   NP_692573.1
- ProteinModelPortal:   Q8EQP2
- SMR:   Q8EQP2
- GeneID:   1018148
- GenomeReviews:   BA000028_GR
- KEGG:   oih:OB1652
- NMPDR:   fig|221109.1.peg.1655
- HOGENOM:   HBG758022
- OMA:   KVEPAHR
- ProtClustDB:   CLSK313238
- BioCyc:   OIHE221109:OB1652-MONOMER
- BRENDA:   1.8.4.8
- GO:   GO:0005737
- HAMAP:   MF_00063
- InterPro:   IPR011798
- InterPro:   IPR004511
- InterPro:   IPR002500
- InterPro:   IPR014729
- Gene3D:   G3DSA:3.40.50.620
- TIGRFAMs:   TIGR02055
- TIGRFAMs:   TIGR00434

Pfam domain/function: PF01507 PAPS_reduct

EC number: =1.8.4.8

Molecular weight: Translated: 27407; Mature: 27276

Theoretical pI: Translated: 4.78; Mature: 4.78

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.9 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
2.9 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGGYSVTYSNFVKDPYKDWFPDDETNGASKILQWAYEVYGTDIVYACSFGAEGMVLIDLI
CCCCEEEHHHHHCCCHHHCCCCCCCCHHHHHHHHHHHHHCCCEEEEECCCCCCEEEEEEH
SKTQKNADIVFLDTDLHFQETYDLIEDIKVKYPTLNIHIKKPDLTLEEQAAEHGSALWKR
HHCCCCCCEEEEECCCCHHHHHHHHHHCEEECCEEEEEEECCCCCHHHHHHHHHHHHHHC
QPDQCCYIRKIKPLEDALSGAPAWISGLRREQSVSRSKTDFINKDERFKSIKVCPLIHWT
CCCCEEEEECCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHCEEEEEEEEECC
WDDVWEYIKDNNLPYNELHDNGYPSIGCIPCTSQVSDSSDSRAGRWAGTQKTECGLHTSD
HHHHHHHHHCCCCCHHHHCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
GGYSVTYSNFVKDPYKDWFPDDETNGASKILQWAYEVYGTDIVYACSFGAEGMVLIDLI
CCCEEEHHHHHCCCHHHCCCCCCCCHHHHHHHHHHHHHCCCEEEEECCCCCCEEEEEEH
SKTQKNADIVFLDTDLHFQETYDLIEDIKVKYPTLNIHIKKPDLTLEEQAAEHGSALWKR
HHCCCCCCEEEEECCCCHHHHHHHHHHCEEECCEEEEEEECCCCCHHHHHHHHHHHHHHC
QPDQCCYIRKIKPLEDALSGAPAWISGLRREQSVSRSKTDFINKDERFKSIKVCPLIHWT
CCCCEEEEECCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHCEEEEEEEEECC
WDDVWEYIKDNNLPYNELHDNGYPSIGCIPCTSQVSDSSDSRAGRWAGTQKTECGLHTSD
HHHHHHHHHCCCCCHHHHCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12235376