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The map label for this gene is glnA [H]

Identifier: 23099106

GI number: 23099106

Start: 1692716

End: 1694053

Strand: Direct

Name: glnA [H]

Synonym: OB1651

Alternate gene names: 23099106

Gene position: 1692716-1694053 (Clockwise)

Preceding gene: 23099105

Following gene: 23099107

Centisome position: 46.62

GC content: 38.04

Gene sequence:

>1338_bases
ATGGGTAAGTCATTTACGAAAGACGAAGTTATCAAGCAAATTAAAGAGGAAAACGTTAAGTTTATTCGATTACAATTTAC
GGATATGCTAGGAACAATTAAAAACGTGGAGATTCCATACAGCCAGCTAGATAAGGCTCTAGATAATAAAATGACGTTTG
ACGGGTCTAGTATTGAAGGTTTTGTACGTATAGAAGAATCTGATATGTTGTTATATCCAGATTTGGATACTTTTGTAGTA
TTTCCTTGGACTTCTGAAAAAGGTAAAGTAGCACGTTTTATTTGTGATATTTATAATCCAGATGGTACTCCGTTTGAAGG
TTGCCCACGTTATAACTTGAAGCGTAACCTTAAAAAGATGGAAGAGTTAGGGTTTGATGCATTCAATATTGGTACAGAAC
CAGAGTTCTTCTTATTTAAATTAGACGAAAAAGGTGAACCAACTCTTGAACTAAATGACCATGGTGGTTATTTTGATCTT
GCACCAACGGACTTGGGTGAAAACTGCCGTCGTGATATCGTACTTGAATTAGAAGAAATGGGCTTTGAAATTGAAGCTTC
TCACCACGAAGTTGCTCCAGGTCAGCATGAAATTGACTTTAAATATTCGGACGCTGTAAAACACGCCGATGACATCCAAA
CTTTTAAACTAGTAGTTAAAACTATTGCTAGAAAGCATAATCTGCACGCTACTTTCATGCCGAAACCATTATTCGGTGTA
AACGGGTCTGGTATGCACGTGAATATGTCCCTATTCAACGGGAAAGAAAATGCATTCTACGACACAAGTGGTGAGCTAGA
GTTGAGCAATACTGCTTATCAGTTTATTGCTGGTATCATTAAGCACGCTACAAACTTTACAGCAGTTACTAACCCTACTG
TAAACTCTTATAAGCGTCTAGTACCAGGTTATGAAGCACCTTGTTATGTTGCTTGGTCTGGTTCTAACCGTAGTCCGTTA
ATTCGTATTCCAAGCTCTCGTGGTCTAAGTACACGTATTGAAGTGCGTAGTGTAGATCCGGCTGCTAACCCATACATGGC
TCTATCAGTACTACTTGCATCAGGATTAGATGGTATTGAACAAGGTTTAGAAGCTCCAGAATCTGTAGATCGCAACATCT
ATGTTATGGATAAAGCTGAGCGTGAAGCTAATGGTGTTCAAGATCTTCCGGCAACATTGAAAGATGCGATGGAAAACTTG
AAAAAAGATAAGATTCTTGTTGATTCTCTTGGTGAACACCTATTCGAACACTTTATTGAAGCAAAAGAAATTGAATGGGA
TATGTTCCGTACAACTGTACACCCTTGGGAACGTGAACAATATTTATCAAATTACTAA

Upstream 100 bases:

>100_bases
AACATCAGTAGTAAATTAGAAAAATGATTGTTACGAAATGTAGCGTAGTAAGATTTTTTCTGATCATAAAAATCTAATTT
AATTTATAGGAGGAGAACGA

Downstream 100 bases:

>100_bases
TCTTGATAAAAAAGTGTATTACACAGGTTGGGTAATTCCGAGTTAGTAGATTCTTTCTACCCGACAATTATTATTACATT
TTGAAGATACTGGCAAAGTA

Product: glutamine synthetase

Products: NA

Alternate protein names: Glutamate--ammonia ligase [H]

Number of amino acids: Translated: 445; Mature: 444

Protein sequence:

>445_residues
MGKSFTKDEVIKQIKEENVKFIRLQFTDMLGTIKNVEIPYSQLDKALDNKMTFDGSSIEGFVRIEESDMLLYPDLDTFVV
FPWTSEKGKVARFICDIYNPDGTPFEGCPRYNLKRNLKKMEELGFDAFNIGTEPEFFLFKLDEKGEPTLELNDHGGYFDL
APTDLGENCRRDIVLELEEMGFEIEASHHEVAPGQHEIDFKYSDAVKHADDIQTFKLVVKTIARKHNLHATFMPKPLFGV
NGSGMHVNMSLFNGKENAFYDTSGELELSNTAYQFIAGIIKHATNFTAVTNPTVNSYKRLVPGYEAPCYVAWSGSNRSPL
IRIPSSRGLSTRIEVRSVDPAANPYMALSVLLASGLDGIEQGLEAPESVDRNIYVMDKAEREANGVQDLPATLKDAMENL
KKDKILVDSLGEHLFEHFIEAKEIEWDMFRTTVHPWEREQYLSNY

Sequences:

>Translated_445_residues
MGKSFTKDEVIKQIKEENVKFIRLQFTDMLGTIKNVEIPYSQLDKALDNKMTFDGSSIEGFVRIEESDMLLYPDLDTFVV
FPWTSEKGKVARFICDIYNPDGTPFEGCPRYNLKRNLKKMEELGFDAFNIGTEPEFFLFKLDEKGEPTLELNDHGGYFDL
APTDLGENCRRDIVLELEEMGFEIEASHHEVAPGQHEIDFKYSDAVKHADDIQTFKLVVKTIARKHNLHATFMPKPLFGV
NGSGMHVNMSLFNGKENAFYDTSGELELSNTAYQFIAGIIKHATNFTAVTNPTVNSYKRLVPGYEAPCYVAWSGSNRSPL
IRIPSSRGLSTRIEVRSVDPAANPYMALSVLLASGLDGIEQGLEAPESVDRNIYVMDKAEREANGVQDLPATLKDAMENL
KKDKILVDSLGEHLFEHFIEAKEIEWDMFRTTVHPWEREQYLSNY
>Mature_444_residues
GKSFTKDEVIKQIKEENVKFIRLQFTDMLGTIKNVEIPYSQLDKALDNKMTFDGSSIEGFVRIEESDMLLYPDLDTFVVF
PWTSEKGKVARFICDIYNPDGTPFEGCPRYNLKRNLKKMEELGFDAFNIGTEPEFFLFKLDEKGEPTLELNDHGGYFDLA
PTDLGENCRRDIVLELEEMGFEIEASHHEVAPGQHEIDFKYSDAVKHADDIQTFKLVVKTIARKHNLHATFMPKPLFGVN
GSGMHVNMSLFNGKENAFYDTSGELELSNTAYQFIAGIIKHATNFTAVTNPTVNSYKRLVPGYEAPCYVAWSGSNRSPLI
RIPSSRGLSTRIEVRSVDPAANPYMALSVLLASGLDGIEQGLEAPESVDRNIYVMDKAEREANGVQDLPATLKDAMENLK
KDKILVDSLGEHLFEHFIEAKEIEWDMFRTTVHPWEREQYLSNY

Specific function: Unknown

COG id: COG0174

COG function: function code E; Glutamine synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamine synthetase family [H]

Homologues:

Organism=Homo sapiens, GI221307485, Length=444, Percent_Identity=27.9279279279279, Blast_Score=147, Evalue=2e-35,
Organism=Escherichia coli, GI1790301, Length=477, Percent_Identity=39.2033542976939, Blast_Score=286, Evalue=1e-78,
Organism=Escherichia coli, GI87081870, Length=468, Percent_Identity=27.991452991453, Blast_Score=147, Evalue=2e-36,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1040 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 15324 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 8,000 Molecules/Cell In: Glucose minimal medi

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008147
- InterPro:   IPR014746
- InterPro:   IPR008146
- InterPro:   IPR004809
- InterPro:   IPR001637 [H]

Pfam domain/function: PF00120 Gln-synt_C; PF03951 Gln-synt_N [H]

EC number: =6.3.1.2 [H]

Molecular weight: Translated: 50518; Mature: 50386

Theoretical pI: Translated: 4.72; Mature: 4.72

Prosite motif: PS00180 GLNA_1 ; PS00181 GLNA_ATP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGKSFTKDEVIKQIKEENVKFIRLQFTDMLGTIKNVEIPYSQLDKALDNKMTFDGSSIEG
CCCCCCHHHHHHHHHHCCCEEEEEEEHHHHCCCCCCCCCHHHHHHHHHCCEEECCCCCEE
FVRIEESDMLLYPDLDTFVVFPWTSEKGKVARFICDIYNPDGTPFEGCPRYNLKRNLKKM
EEEEECCCEEEECCCCCEEEEEEECCCCCEEEEEEEEECCCCCCCCCCCCCHHHHHHHHH
EELGFDAFNIGTEPEFFLFKLDEKGEPTLELNDHGGYFDLAPTDLGENCRRDIVLELEEM
HHCCCCEEECCCCCCEEEEEECCCCCCEEEECCCCCEEECCCCCCCHHHHHHHEEEHHHC
GFEIEASHHEVAPGQHEIDFKYSDAVKHADDIQTFKLVVKTIARKHNLHATFMPKPLFGV
CEEEECCCCCCCCCCCEECEEHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCC
NGSGMHVNMSLFNGKENAFYDTSGELELSNTAYQFIAGIIKHATNFTAVTNPTVNSYKRL
CCCCEEEEEEEECCCCCCEECCCCCEEECHHHHHHHHHHHHHCCCCEEECCCCHHHHHHH
VPGYEAPCYVAWSGSNRSPLIRIPSSRGLSTRIEVRSVDPAANPYMALSVLLASGLDGIE
CCCCCCCEEEEECCCCCCCEEECCCCCCCEEEEEEEECCCCCCHHHHHHHHHHHCCHHHH
QGLEAPESVDRNIYVMDKAEREANGVQDLPATLKDAMENLKKDKILVDSLGEHLFEHFIE
HHCCCHHHHCCEEEEEECCHHHCCCCHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHH
AKEIEWDMFRTTVHPWEREQYLSNY
HHHCCHHHHHHCCCCCHHHHHHCCC
>Mature Secondary Structure 
GKSFTKDEVIKQIKEENVKFIRLQFTDMLGTIKNVEIPYSQLDKALDNKMTFDGSSIEG
CCCCCHHHHHHHHHHCCCEEEEEEEHHHHCCCCCCCCCHHHHHHHHHCCEEECCCCCEE
FVRIEESDMLLYPDLDTFVVFPWTSEKGKVARFICDIYNPDGTPFEGCPRYNLKRNLKKM
EEEEECCCEEEECCCCCEEEEEEECCCCCEEEEEEEEECCCCCCCCCCCCCHHHHHHHHH
EELGFDAFNIGTEPEFFLFKLDEKGEPTLELNDHGGYFDLAPTDLGENCRRDIVLELEEM
HHCCCCEEECCCCCCEEEEEECCCCCCEEEECCCCCEEECCCCCCCHHHHHHHEEEHHHC
GFEIEASHHEVAPGQHEIDFKYSDAVKHADDIQTFKLVVKTIARKHNLHATFMPKPLFGV
CEEEECCCCCCCCCCCEECEEHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCC
NGSGMHVNMSLFNGKENAFYDTSGELELSNTAYQFIAGIIKHATNFTAVTNPTVNSYKRL
CCCCEEEEEEEECCCCCCEECCCCCEEECHHHHHHHHHHHHHCCCCEEECCCCHHHHHHH
VPGYEAPCYVAWSGSNRSPLIRIPSSRGLSTRIEVRSVDPAANPYMALSVLLASGLDGIE
CCCCCCCEEEEECCCCCCCEEECCCCCCCEEEEEEEECCCCCCHHHHHHHHHHHCCHHHH
QGLEAPESVDRNIYVMDKAEREANGVQDLPATLKDAMENLKKDKILVDSLGEHLFEHFIE
HHCCCHHHHCCEEEEEECCHHHCCCCHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHH
AKEIEWDMFRTTVHPWEREQYLSNY
HHHCCHHHHHHCCCCCHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2906311; 2565853; 9384377; 11719184 [H]