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The map label for this gene is cysJ [H]

Identifier: 23099108

GI number: 23099108

Start: 1695414

End: 1697255

Strand: Direct

Name: cysJ [H]

Synonym: OB1653

Alternate gene names: 23099108

Gene position: 1695414-1697255 (Clockwise)

Preceding gene: 23099107

Following gene: 23099109

Centisome position: 46.7

GC content: 39.69

Gene sequence:

>1842_bases
TTGCTGTTACAAGAAACAAACAGTCCGTTTAATAAAGAACAGTCTGACTATCTTAATAAGTTACTACCGACGTTAACGGA
GAGTCAGCGTATTTGGCTAAGTGGTTACCTGTCAGCTAAACAGTTAGCTGACAGTGATACCAAAGCCCAACAATCAACCA
ATCCGATTTTAGCCCATGAACAGTCATTAAAGCCAGTCAATGAAACAAAGTTAAGGGATGTAACCATTCTTTATGGTTCA
GAGACTGGAAATGGAGAGACAATTGCAAAGGACTTGGGAGAAAAAGTAGAATCAAGAGATTTTAAAGTCACTATTTCCTC
TATGGATAACTTTAAAGCGAAGGATCTTAAGAAAGTGCAGGATATTTTCATCATTACAGCAACGCATGGTGAAGGTGATC
CTCCTGAGAATGCAATATCTTTTCATGAATTTCTCCATGGTAGAAAAGCACCGAAACTGAAAGATGTTAGGTTCTCTGTT
CTCTCACTTGGTGATCAGTCGTATGAATTCTTTTGCCAGACTGGAAAGGAGTTTGATAATCGTTTAGAAGAGTTAGGAGG
GGATAGGCTCTATCCGAGAGTAGATTGTGATGTGGATTTTGATGACCTGGCAGCTGAATGGATAGACGGCGTACTTCATG
AATTATCTAAAGCAAAATCCGCGCATGATCCAGAGTTATCCGAGACCTCTACAGAAATTGATACGGAAGGTCCAGTCTAT
TCAAGAACGAATCCATTCCACGCGGAAGTGTTGGAGAATTTGAATCTTAATGGTGAAGGATCCAATAAAGAGACGTATCA
TATGGAGTTATCTATTGAGGATGCAAATTTTGAATTTGAGATAGGGGACAGTCTCGGTATTTATCCTAAAAATAATCCTG
ATATGGTGGAGCAGCTTATCACTGGGTTGAATTTCGAACCTGAAGGAAGTGTATCGATTAACAAACAAGGAGAAAGACGA
TCGTTACGAGAAGCATTGTTGACCAATTTTGAAATATCTAGAATCACAAAACCTTTATTGGAAAAAGCTGCACAGTATTT
CAATAATAATAATCTAAAGGATCTTGTAACGCCGGAAAAAGTCGAAGAACAAAAAGCCTATATCCAAGGCAGGGATGTCA
TCGATTTAATTACGGATTATCCGCCAGTGGATATGACACCAGAAGAATTTATTCAGATTCTAAGGAAAATGCCGGCAAGA
TTGTATTCTATTTCAAGCAGTTTTAGAGCAAATCCGGATGAAGTACATATCACTGTCGCAAAAGCCATTTATACAGGTAA
CAGGCAAGAACAGGCTGGTGTTTGCTCTGGACAATGTGCGGAAAGGGTAACCCCTGGAGATCTATTGCCGATATACATCC
ATCGTAATCCGAATTTTAAGTTTCCAAATGATGTTGATACACCTGTAATCATGATTGGACCTGGCACAGGAGTGGCACCC
TACAGATCCCTGCTAGAGGAACGTGAAGAAATAGGAGCAAATGGCAGAACTTGGCTATTTTATGGGGATCAGCATTTTGC
CACTGACTTTCTCTATCAAGTAGAATGGCAAAACTGGCTGAAAAAAGGAGTACTGACCAAGATGGATGTGGCCTTCTCGC
GAGACAGTGCGGATAAAGTGTATGTGCAACATCGAATGTTGGAGCATAGCAAGGAACTTTTTCAATGGTTGCAAGATGGA
GCAAATATCTATGTATGTGGAGATGAAAAGTATATGGCAAATGATGTTCACCAAATGCTTTCAACAATTATTCAAAAGGA
AGGAAGATACAGTTTTGAGGAAGCAGAAGCATATTTGACTGATATGCGAAAACAGAAACGATACCAACGAGACGTGTATT
GA

Upstream 100 bases:

>100_bases
TTTTAAGAAGATGCCTTTTTTATAAAATCCGAGTAAATTGATATGTAAACTTATGAAATTAAAAATTACTTGATGTATCT
ATAACGTGAGGTGGAATATA

Downstream 100 bases:

>100_bases
AATAAGTATACGAAAAAGGAGTTGAAAGAGTGACGAAATCTAATATTTCTAAAGATAACGGTCCATTGGATGAAGTGGAG
GGTATTAAGGAGAGAAGTAA

Product: sulfite (NADPH) reductase flavoprotein

Products: NA

Alternate protein names: SiR-FP [H]

Number of amino acids: Translated: 613; Mature: 613

Protein sequence:

>613_residues
MLLQETNSPFNKEQSDYLNKLLPTLTESQRIWLSGYLSAKQLADSDTKAQQSTNPILAHEQSLKPVNETKLRDVTILYGS
ETGNGETIAKDLGEKVESRDFKVTISSMDNFKAKDLKKVQDIFIITATHGEGDPPENAISFHEFLHGRKAPKLKDVRFSV
LSLGDQSYEFFCQTGKEFDNRLEELGGDRLYPRVDCDVDFDDLAAEWIDGVLHELSKAKSAHDPELSETSTEIDTEGPVY
SRTNPFHAEVLENLNLNGEGSNKETYHMELSIEDANFEFEIGDSLGIYPKNNPDMVEQLITGLNFEPEGSVSINKQGERR
SLREALLTNFEISRITKPLLEKAAQYFNNNNLKDLVTPEKVEEQKAYIQGRDVIDLITDYPPVDMTPEEFIQILRKMPAR
LYSISSSFRANPDEVHITVAKAIYTGNRQEQAGVCSGQCAERVTPGDLLPIYIHRNPNFKFPNDVDTPVIMIGPGTGVAP
YRSLLEEREEIGANGRTWLFYGDQHFATDFLYQVEWQNWLKKGVLTKMDVAFSRDSADKVYVQHRMLEHSKELFQWLQDG
ANIYVCGDEKYMANDVHQMLSTIIQKEGRYSFEEAEAYLTDMRKQKRYQRDVY

Sequences:

>Translated_613_residues
MLLQETNSPFNKEQSDYLNKLLPTLTESQRIWLSGYLSAKQLADSDTKAQQSTNPILAHEQSLKPVNETKLRDVTILYGS
ETGNGETIAKDLGEKVESRDFKVTISSMDNFKAKDLKKVQDIFIITATHGEGDPPENAISFHEFLHGRKAPKLKDVRFSV
LSLGDQSYEFFCQTGKEFDNRLEELGGDRLYPRVDCDVDFDDLAAEWIDGVLHELSKAKSAHDPELSETSTEIDTEGPVY
SRTNPFHAEVLENLNLNGEGSNKETYHMELSIEDANFEFEIGDSLGIYPKNNPDMVEQLITGLNFEPEGSVSINKQGERR
SLREALLTNFEISRITKPLLEKAAQYFNNNNLKDLVTPEKVEEQKAYIQGRDVIDLITDYPPVDMTPEEFIQILRKMPAR
LYSISSSFRANPDEVHITVAKAIYTGNRQEQAGVCSGQCAERVTPGDLLPIYIHRNPNFKFPNDVDTPVIMIGPGTGVAP
YRSLLEEREEIGANGRTWLFYGDQHFATDFLYQVEWQNWLKKGVLTKMDVAFSRDSADKVYVQHRMLEHSKELFQWLQDG
ANIYVCGDEKYMANDVHQMLSTIIQKEGRYSFEEAEAYLTDMRKQKRYQRDVY
>Mature_613_residues
MLLQETNSPFNKEQSDYLNKLLPTLTESQRIWLSGYLSAKQLADSDTKAQQSTNPILAHEQSLKPVNETKLRDVTILYGS
ETGNGETIAKDLGEKVESRDFKVTISSMDNFKAKDLKKVQDIFIITATHGEGDPPENAISFHEFLHGRKAPKLKDVRFSV
LSLGDQSYEFFCQTGKEFDNRLEELGGDRLYPRVDCDVDFDDLAAEWIDGVLHELSKAKSAHDPELSETSTEIDTEGPVY
SRTNPFHAEVLENLNLNGEGSNKETYHMELSIEDANFEFEIGDSLGIYPKNNPDMVEQLITGLNFEPEGSVSINKQGERR
SLREALLTNFEISRITKPLLEKAAQYFNNNNLKDLVTPEKVEEQKAYIQGRDVIDLITDYPPVDMTPEEFIQILRKMPAR
LYSISSSFRANPDEVHITVAKAIYTGNRQEQAGVCSGQCAERVTPGDLLPIYIHRNPNFKFPNDVDTPVIMIGPGTGVAP
YRSLLEEREEIGANGRTWLFYGDQHFATDFLYQVEWQNWLKKGVLTKMDVAFSRDSADKVYVQHRMLEHSKELFQWLQDG
ANIYVCGDEKYMANDVHQMLSTIIQKEGRYSFEEAEAYLTDMRKQKRYQRDVY

Specific function: Component of the sulfite reductase complex that catalyzes the 6-electron reduction of sulfite to sulfide. This is one of several activities required for the biosynthesis of L- cysteine from sulfate. The flavoprotein component catalyzes the electron flow f

COG id: COG0369

COG function: function code P; Sulfite reductase, alpha subunit (flavoprotein)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 flavodoxin-like domain [H]

Homologues:

Organism=Homo sapiens, GI127139033, Length=627, Percent_Identity=28.2296650717703, Blast_Score=247, Evalue=2e-65,
Organism=Homo sapiens, GI7657393, Length=595, Percent_Identity=26.890756302521, Blast_Score=207, Evalue=3e-53,
Organism=Homo sapiens, GI221316705, Length=604, Percent_Identity=26.4900662251656, Blast_Score=199, Evalue=6e-51,
Organism=Homo sapiens, GI24041029, Length=595, Percent_Identity=29.0756302521008, Blast_Score=197, Evalue=2e-50,
Organism=Homo sapiens, GI221316709, Length=597, Percent_Identity=26.6331658291457, Blast_Score=196, Evalue=5e-50,
Organism=Homo sapiens, GI10835173, Length=653, Percent_Identity=27.4119448698315, Blast_Score=195, Evalue=1e-49,
Organism=Homo sapiens, GI40254422, Length=645, Percent_Identity=26.5116279069767, Blast_Score=188, Evalue=1e-47,
Organism=Homo sapiens, GI221316707, Length=519, Percent_Identity=27.3603082851638, Blast_Score=180, Evalue=3e-45,
Organism=Homo sapiens, GI169790958, Length=444, Percent_Identity=27.7027027027027, Blast_Score=149, Evalue=9e-36,
Organism=Homo sapiens, GI169790956, Length=444, Percent_Identity=27.7027027027027, Blast_Score=149, Evalue=1e-35,
Organism=Escherichia coli, GI1789123, Length=606, Percent_Identity=37.4587458745875, Blast_Score=419, Evalue=1e-118,
Organism=Escherichia coli, GI1790181, Length=142, Percent_Identity=30.9859154929577, Blast_Score=70, Evalue=6e-13,
Organism=Caenorhabditis elegans, GI17554134, Length=597, Percent_Identity=29.8157453936348, Blast_Score=266, Evalue=2e-71,
Organism=Caenorhabditis elegans, GI17566446, Length=576, Percent_Identity=27.4305555555556, Blast_Score=163, Evalue=2e-40,
Organism=Caenorhabditis elegans, GI17531441, Length=316, Percent_Identity=22.4683544303797, Blast_Score=85, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI32563863, Length=130, Percent_Identity=31.5384615384615, Blast_Score=68, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6321832, Length=641, Percent_Identity=27.613104524181, Blast_Score=195, Evalue=2e-50,
Organism=Saccharomyces cerevisiae, GI6321143, Length=390, Percent_Identity=27.9487179487179, Blast_Score=149, Evalue=2e-36,
Organism=Saccharomyces cerevisiae, GI6325305, Length=628, Percent_Identity=23.0891719745223, Blast_Score=130, Evalue=7e-31,
Organism=Saccharomyces cerevisiae, GI6322597, Length=196, Percent_Identity=27.0408163265306, Blast_Score=67, Evalue=6e-12,
Organism=Drosophila melanogaster, GI17137192, Length=607, Percent_Identity=29.9835255354201, Blast_Score=245, Evalue=5e-65,
Organism=Drosophila melanogaster, GI24582192, Length=526, Percent_Identity=30.0380228136882, Blast_Score=210, Evalue=2e-54,
Organism=Drosophila melanogaster, GI24583543, Length=531, Percent_Identity=29.1902071563089, Blast_Score=156, Evalue=5e-38,
Organism=Drosophila melanogaster, GI78706876, Length=531, Percent_Identity=29.1902071563089, Blast_Score=156, Evalue=5e-38,
Organism=Drosophila melanogaster, GI24660907, Length=591, Percent_Identity=23.1810490693739, Blast_Score=156, Evalue=5e-38,
Organism=Drosophila melanogaster, GI24660903, Length=591, Percent_Identity=23.1810490693739, Blast_Score=156, Evalue=5e-38,
Organism=Drosophila melanogaster, GI78706872, Length=531, Percent_Identity=29.1902071563089, Blast_Score=155, Evalue=5e-38,
Organism=Drosophila melanogaster, GI24647438, Length=412, Percent_Identity=24.0291262135922, Blast_Score=88, Evalue=2e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010199
- InterPro:   IPR003097
- InterPro:   IPR017927
- InterPro:   IPR001094
- InterPro:   IPR008254
- InterPro:   IPR001709
- InterPro:   IPR023173
- InterPro:   IPR001433
- InterPro:   IPR017938 [H]

Pfam domain/function: PF00667 FAD_binding_1; PF00258 Flavodoxin_1; PF00175 NAD_binding_1 [H]

EC number: =1.8.1.2 [H]

Molecular weight: Translated: 69948; Mature: 69948

Theoretical pI: Translated: 4.65; Mature: 4.65

Prosite motif: PS50902 FLAVODOXIN_LIKE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLLQETNSPFNKEQSDYLNKLLPTLTESQRIWLSGYLSAKQLADSDTKAQQSTNPILAHE
CCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEECCCHHHHHCCCCHHHHHCCCCCEECC
QSLKPVNETKLRDVTILYGSETGNGETIAKDLGEKVESRDFKVTISSMDNFKAKDLKKVQ
CCCCCCCCCCCEEEEEEEECCCCCCHHHHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHC
DIFIITATHGEGDPPENAISFHEFLHGRKAPKLKDVRFSVLSLGDQSYEFFCQTGKEFDN
EEEEEEEECCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCHHHHHHHCCHHHHH
RLEELGGDRLYPRVDCDVDFDDLAAEWIDGVLHELSKAKSAHDPELSETSTEIDTEGPVY
HHHHCCCCCCCCEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCC
SRTNPFHAEVLENLNLNGEGSNKETYHMELSIEDANFEFEIGDSLGIYPKNNPDMVEQLI
CCCCCHHHHHHHCCCCCCCCCCCEEEEEEEEEECCCEEEEECCCCCCCCCCCHHHHHHHH
TGLNFEPEGSVSINKQGERRSLREALLTNFEISRITKPLLEKAAQYFNNNNLKDLVTPEK
HCCCCCCCCCEEECCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCHH
VEEQKAYIQGRDVIDLITDYPPVDMTPEEFIQILRKMPARLYSISSSFRANPDEVHITVA
HHHHHHHHCCCCHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEE
KAIYTGNRQEQAGVCSGQCAERVTPGDLLPIYIHRNPNFKFPNDVDTPVIMIGPGTGVAP
EEHHCCCCCHHCCCCCCHHHHCCCCCCEEEEEEECCCCCCCCCCCCCCEEEEECCCCCHH
YRSLLEEREEIGANGRTWLFYGDQHFATDFLYQVEWQNWLKKGVLTKMDVAFSRDSADKV
HHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCHHH
YVQHRMLEHSKELFQWLQDGANIYVCGDEKYMANDVHQMLSTIIQKEGRYSFEEAEAYLT
HHHHHHHHHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
DMRKQKRYQRDVY
HHHHHHHHHHCCC
>Mature Secondary Structure
MLLQETNSPFNKEQSDYLNKLLPTLTESQRIWLSGYLSAKQLADSDTKAQQSTNPILAHE
CCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEECCCHHHHHCCCCHHHHHCCCCCEECC
QSLKPVNETKLRDVTILYGSETGNGETIAKDLGEKVESRDFKVTISSMDNFKAKDLKKVQ
CCCCCCCCCCCEEEEEEEECCCCCCHHHHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHC
DIFIITATHGEGDPPENAISFHEFLHGRKAPKLKDVRFSVLSLGDQSYEFFCQTGKEFDN
EEEEEEEECCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCHHHHHHHCCHHHHH
RLEELGGDRLYPRVDCDVDFDDLAAEWIDGVLHELSKAKSAHDPELSETSTEIDTEGPVY
HHHHCCCCCCCCEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCC
SRTNPFHAEVLENLNLNGEGSNKETYHMELSIEDANFEFEIGDSLGIYPKNNPDMVEQLI
CCCCCHHHHHHHCCCCCCCCCCCEEEEEEEEEECCCEEEEECCCCCCCCCCCHHHHHHHH
TGLNFEPEGSVSINKQGERRSLREALLTNFEISRITKPLLEKAAQYFNNNNLKDLVTPEK
HCCCCCCCCCEEECCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCHH
VEEQKAYIQGRDVIDLITDYPPVDMTPEEFIQILRKMPARLYSISSSFRANPDEVHITVA
HHHHHHHHCCCCHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEE
KAIYTGNRQEQAGVCSGQCAERVTPGDLLPIYIHRNPNFKFPNDVDTPVIMIGPGTGVAP
EEHHCCCCCHHCCCCCCHHHHCCCCCCEEEEEEECCCCCCCCCCCCCCEEEEECCCCCHH
YRSLLEEREEIGANGRTWLFYGDQHFATDFLYQVEWQNWLKKGVLTKMDVAFSRDSADKV
HHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCHHH
YVQHRMLEHSKELFQWLQDGANIYVCGDEKYMANDVHQMLSTIIQKEGRYSFEEAEAYLT
HHHHHHHHHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
DMRKQKRYQRDVY
HHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]