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The map label for this gene is gatB

Identifier: 23098221

GI number: 23098221

Start: 817766

End: 819196

Strand: Direct

Name: gatB

Synonym: OB0766

Alternate gene names: 23098221

Gene position: 817766-819196 (Clockwise)

Preceding gene: 23098220

Following gene: 23098222

Centisome position: 22.52

GC content: 35.92

Gene sequence:

>1431_bases
ATGAATTTCGAAACAATAATAGGTCTAGAAGTACACGTAGAATTAAAAACAAACTCAAAGATTTTTAGCCCAAGTACAAA
TGAATTTGGCTCTGATCCGAATACCAATGTGAACCCGATTGACTTAGGCTATCCAGGTACATTACCTGTACTTAATGAAG
AGGCGGTTAACTTTGCAATGAAGGCTGCAATGGCTCTAAATTGTGAAATCGCAACGGATACAAAATTTGACCGTAAAAAC
TATTTCTATCCGGATAATCCGAAAGCTTACCAAATCTCTCAATTCGATCAACCAATAGGCGAAAACGGATGGATTGAAAT
TGAAGTAAATGGTGAGAAGAAGAAAATTGGTATCACTCGTCTACATTTAGAAGAAGATGCTGGTAAATTAACACATGGTG
AAGATGGATATTCTTATGTCGACTTTAACCGTCAAGGTACGCCGTTAATCGAGATTGTATCGGAACCAGATATGCGTTCT
CCAGAAGAAGCATATGCATATTTAGAAAAACTAAAAAATATTATTCAATATACTGGAGTTTCTGATGTGAAGATGCAGGA
AGGTTCTTTACGTTGTGATGCGAATATTTCCTTACGTCCAATTGGACAAGAAGAATTTGGTACAAAAGCGGAATTGAAAA
ACTTAAACTCCTTCTCTTATGTTCAAAAAGGATTAGAGTTTGAAGAAAAGCGTCAGGAAAAAGAATTGTTATCAGGTGGA
GAGATTCTACAAGAAACTCGCCGTTATGATGAGAAGACAAAAGAAACCATTCTTATGCGTGTTAAAGAAGGTTCGGATGA
TTATCGTTATTTCCCAGAGCCAGATCTAGTTCCACTATATATTGATGAAGAATGGAAAGAGCGTGTAAGAAGCGAAATCC
CTGAATTACCAGATGCACGTCTGGAACGTTATATCAATGAACTTGGACTTTCAGAGTATGATGCCAACGTGTTAACAGCT
TCTAAGCAAATGTCTGATTTCTTTGAAGAAGCAACAGCAGAAGGTGCTGATATGAAGCAAGTCTCAAATTGGTTAATGGG
TGAAGTTTCTGCATATATGAATAAGCATTATAAAGAGTTGGACGAACTGGCAATTACACCAGAAGCACTTGCGAAAATGA
TTAATCTAATTGAAGATGGAACCATTTCTTCTAAAATAGCTAAAAAAGTATTTGCAGAGCTAGTGGAAAATGGTGGCGAT
CCTCAGAAAATTGTAGAAGAAAAAGGGCTCGTACAAATTTCTGATCCAGCACAATTAAAACAGATTGTGAATGAAGTACT
AGATCAAAATGAACAGTCTATTATTGACTTTAAGAATGGAAAGAACAAAGCAAAAGGCTTCTTAGTTGGACAAATTATGA
AAGCAACAAAAGGACAAGCAAATCCACCATTAGTAAATAAAATTCTTGCAGAAGAACTTAATAATCGCTAA

Upstream 100 bases:

>100_bases
GTAACTATTTCGATGAAAGCACAGTATACCGTACAGCTTACGCATATGAACAAGCAACGGAACATCACAAGAAAAGACCT
CAACTTGGAGGTGCAAAATA

Downstream 100 bases:

>100_bases
TACTCTAAGAGAGGCTGTCATTATAAATTAATGGCAGCCTACAAAATTATCAAATATCCTTTTCAAACTATAAAAAAAGG
ACTATGATATTGGATAGGCA

Product: aspartyl/glutamyl-tRNA amidotransferase subunit B

Products: NA

Alternate protein names: Asp/Glu-ADT subunit B

Number of amino acids: Translated: 476; Mature: 476

Protein sequence:

>476_residues
MNFETIIGLEVHVELKTNSKIFSPSTNEFGSDPNTNVNPIDLGYPGTLPVLNEEAVNFAMKAAMALNCEIATDTKFDRKN
YFYPDNPKAYQISQFDQPIGENGWIEIEVNGEKKKIGITRLHLEEDAGKLTHGEDGYSYVDFNRQGTPLIEIVSEPDMRS
PEEAYAYLEKLKNIIQYTGVSDVKMQEGSLRCDANISLRPIGQEEFGTKAELKNLNSFSYVQKGLEFEEKRQEKELLSGG
EILQETRRYDEKTKETILMRVKEGSDDYRYFPEPDLVPLYIDEEWKERVRSEIPELPDARLERYINELGLSEYDANVLTA
SKQMSDFFEEATAEGADMKQVSNWLMGEVSAYMNKHYKELDELAITPEALAKMINLIEDGTISSKIAKKVFAELVENGGD
PQKIVEEKGLVQISDPAQLKQIVNEVLDQNEQSIIDFKNGKNKAKGFLVGQIMKATKGQANPPLVNKILAEELNNR

Sequences:

>Translated_476_residues
MNFETIIGLEVHVELKTNSKIFSPSTNEFGSDPNTNVNPIDLGYPGTLPVLNEEAVNFAMKAAMALNCEIATDTKFDRKN
YFYPDNPKAYQISQFDQPIGENGWIEIEVNGEKKKIGITRLHLEEDAGKLTHGEDGYSYVDFNRQGTPLIEIVSEPDMRS
PEEAYAYLEKLKNIIQYTGVSDVKMQEGSLRCDANISLRPIGQEEFGTKAELKNLNSFSYVQKGLEFEEKRQEKELLSGG
EILQETRRYDEKTKETILMRVKEGSDDYRYFPEPDLVPLYIDEEWKERVRSEIPELPDARLERYINELGLSEYDANVLTA
SKQMSDFFEEATAEGADMKQVSNWLMGEVSAYMNKHYKELDELAITPEALAKMINLIEDGTISSKIAKKVFAELVENGGD
PQKIVEEKGLVQISDPAQLKQIVNEVLDQNEQSIIDFKNGKNKAKGFLVGQIMKATKGQANPPLVNKILAEELNNR
>Mature_476_residues
MNFETIIGLEVHVELKTNSKIFSPSTNEFGSDPNTNVNPIDLGYPGTLPVLNEEAVNFAMKAAMALNCEIATDTKFDRKN
YFYPDNPKAYQISQFDQPIGENGWIEIEVNGEKKKIGITRLHLEEDAGKLTHGEDGYSYVDFNRQGTPLIEIVSEPDMRS
PEEAYAYLEKLKNIIQYTGVSDVKMQEGSLRCDANISLRPIGQEEFGTKAELKNLNSFSYVQKGLEFEEKRQEKELLSGG
EILQETRRYDEKTKETILMRVKEGSDDYRYFPEPDLVPLYIDEEWKERVRSEIPELPDARLERYINELGLSEYDANVLTA
SKQMSDFFEEATAEGADMKQVSNWLMGEVSAYMNKHYKELDELAITPEALAKMINLIEDGTISSKIAKKVFAELVENGGD
PQKIVEEKGLVQISDPAQLKQIVNEVLDQNEQSIIDFKNGKNKAKGFLVGQIMKATKGQANPPLVNKILAEELNNR

Specific function: Allows the formation of correctly charged Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation of misacylated Asp- tRNA(Asn) or Glu-tRNA(Gln) in organisms which lack either or both of asparaginyl-tRNA or glutaminyl-tRNA synthetases. The reaction take

COG id: COG0064

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase B subunit (PET112 homolog)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gatB/gatE family. GatB subfamily

Homologues:

Organism=Homo sapiens, GI4758894, Length=495, Percent_Identity=37.1717171717172, Blast_Score=313, Evalue=3e-85,
Organism=Caenorhabditis elegans, GI115534094, Length=304, Percent_Identity=37.171052631579, Blast_Score=201, Evalue=9e-52,
Organism=Saccharomyces cerevisiae, GI6319391, Length=514, Percent_Identity=30.9338521400778, Blast_Score=226, Evalue=6e-60,
Organism=Drosophila melanogaster, GI45550808, Length=485, Percent_Identity=31.7525773195876, Blast_Score=254, Evalue=7e-68,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GATB_OCEIH (Q8ES77)

Other databases:

- EMBL:   BA000028
- RefSeq:   NP_691687.1
- ProteinModelPortal:   Q8ES77
- SMR:   Q8ES77
- GeneID:   1016297
- GenomeReviews:   BA000028_GR
- KEGG:   oih:OB0766
- NMPDR:   fig|221109.1.peg.774
- HOGENOM:   HBG395951
- OMA:   LRYLGTC
- ProtClustDB:   PRK05477
- BioCyc:   OIHE221109:OB0766-MONOMER
- GO:   GO:0006412
- HAMAP:   MF_00121
- InterPro:   IPR017959
- InterPro:   IPR018027
- InterPro:   IPR003789
- InterPro:   IPR004413
- InterPro:   IPR006075
- InterPro:   IPR017958
- PANTHER:   PTHR11659
- SMART:   SM00845
- TIGRFAMs:   TIGR00133

Pfam domain/function: PF02934 GatB_N; PF02637 GatB_Yqey; SSF89095 GatB_Yqey

EC number: 6.3.5.-

Molecular weight: Translated: 53912; Mature: 53912

Theoretical pI: Translated: 4.42; Mature: 4.42

Prosite motif: PS01234 GATB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNFETIIGLEVHVELKTNSKIFSPSTNEFGSDPNTNVNPIDLGYPGTLPVLNEEAVNFAM
CCCCEEEEEEEEEEEECCCEEECCCCCCCCCCCCCCCCEEECCCCCCCCCCCHHHHHHHH
KAAMALNCEIATDTKFDRKNYFYPDNPKAYQISQFDQPIGENGWIEIEVNGEKKKIGITR
HHHHHEEEEEECCCCCCCCCCCCCCCCCEEEHHHHCCCCCCCCEEEEEECCCEEEECEEE
LHLEEDAGKLTHGEDGYSYVDFNRQGTPLIEIVSEPDMRSPEEAYAYLEKLKNIIQYTGV
EEEECCCCCCCCCCCCCEEEECCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCC
SDVKMQEGSLRCDANISLRPIGQEEFGTKAELKNLNSFSYVQKGLEFEEKRQEKELLSGG
CCCEECCCCEEECCCCEEEECCHHHCCCHHHHHCCCHHHHHHHCCCHHHHHHHHHHHCCH
EILQETRRYDEKTKETILMRVKEGSDDYRYFPEPDLVPLYIDEEWKERVRSEIPELPDAR
HHHHHHHHHHHHHHHHHEEEEECCCCCCCCCCCCCEEEEEECHHHHHHHHHHCCCCCHHH
LERYINELGLSEYDANVLTASKQMSDFFEEATAEGADMKQVSNWLMGEVSAYMNKHYKEL
HHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHH
DELAITPEALAKMINLIEDGTISSKIAKKVFAELVENGGDPQKIVEEKGLVQISDPAQLK
HHHCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHCCCEEECCHHHHH
QIVNEVLDQNEQSIIDFKNGKNKAKGFLVGQIMKATKGQANPPLVNKILAEELNNR
HHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCC
>Mature Secondary Structure
MNFETIIGLEVHVELKTNSKIFSPSTNEFGSDPNTNVNPIDLGYPGTLPVLNEEAVNFAM
CCCCEEEEEEEEEEEECCCEEECCCCCCCCCCCCCCCCEEECCCCCCCCCCCHHHHHHHH
KAAMALNCEIATDTKFDRKNYFYPDNPKAYQISQFDQPIGENGWIEIEVNGEKKKIGITR
HHHHHEEEEEECCCCCCCCCCCCCCCCCEEEHHHHCCCCCCCCEEEEEECCCEEEECEEE
LHLEEDAGKLTHGEDGYSYVDFNRQGTPLIEIVSEPDMRSPEEAYAYLEKLKNIIQYTGV
EEEECCCCCCCCCCCCCEEEECCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCC
SDVKMQEGSLRCDANISLRPIGQEEFGTKAELKNLNSFSYVQKGLEFEEKRQEKELLSGG
CCCEECCCCEEECCCCEEEECCHHHCCCHHHHHCCCHHHHHHHCCCHHHHHHHHHHHCCH
EILQETRRYDEKTKETILMRVKEGSDDYRYFPEPDLVPLYIDEEWKERVRSEIPELPDAR
HHHHHHHHHHHHHHHHHEEEEECCCCCCCCCCCCCEEEEEECHHHHHHHHHHCCCCCHHH
LERYINELGLSEYDANVLTASKQMSDFFEEATAEGADMKQVSNWLMGEVSAYMNKHYKEL
HHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHH
DELAITPEALAKMINLIEDGTISSKIAKKVFAELVENGGDPQKIVEEKGLVQISDPAQLK
HHHCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHCCCEEECCHHHHH
QIVNEVLDQNEQSIIDFKNGKNKAKGFLVGQIMKATKGQANPPLVNKILAEELNNR
HHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12235376