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The map label for this gene is gatA

Identifier: 23098220

GI number: 23098220

Start: 816300

End: 817766

Strand: Direct

Name: gatA

Synonym: OB0765

Alternate gene names: 23098220

Gene position: 816300-817766 (Clockwise)

Preceding gene: 23098219

Following gene: 23098221

Centisome position: 22.48

GC content: 37.63

Gene sequence:

>1467_bases
ATGTCTTTATTTGATTACACTATAAAAGAATTAGAAGAAAAGCTACATAATAAAGAAATTACGGTTGAAGATCTAGTTAA
AGAATCCTATCAACGTATTCATGAGGTAGATCAAGAGGTCCATGCTTTTCTTACGTTAGATGAAGAAAAAGCGTTAGAAA
AAGCGAGAGAATTAGATCAACTTGAAGATAAAACAGGTATCTTATTTGGAATGCCTGGTGGTATTAAAGATAATATTGTA
ACAAAAGGACTTCGTACAACTTGTGCAAGTCAATTCTTAGATAATTTCAATGATCCATTATATGATGCGACTGTTGTTCA
AAAGTTACAGAAAGAAAACATGGTTGCTGTTGGTAAGTTGAATATGGATGAATTTGCCATGGGCTCTTCCAATGAAAACT
CTGGTTATGAACCAACTCGTAATCCTTGGAATACAGAACACGTACCAGGTGGATCTAGTGGTGGTTCTGCTGCCGCAGTA
GCTGCAGGAGAGGTACTATTCACATTAGGCTCTGATACGGGTGGTTCAATTCGTCAACCAGCAGCATTCTGTGGAGTAGT
CGGTATGAAACCAACGTACGGAAGAGTTTCACGTTTTGGATTGGTTGCTTTTGCATCTTCTCTTGATCAGATTGGACCAG
TTACTCGTACAGTAGAAGATAATGCACGCGTACTTGAAGTTATTGCTGGGCATGACCAAATGGATACAACTAGTGCGAAT
GTAGAAGTTCCTTCCTATACAGAAGCGTTAAAACAAGATGTAAAAGGACTTAAAATCGCTGTACCAAAAGAATATCTTGC
AGAAGGTGTATCGAAAGAAGTAAAAGACGCGATTTTAGAAGCATTAAAGGTATATGAATCCATGGGTGCTACATGGGAAG
AAGTTTCCTTGCCGCATTCTAAATATGCACTAGCAACTTACTATTTACTTTCATCTTCAGAGGCGTCTGCTAACTTAGCT
CGTTTTGATGGCGTACGTTACGGTGTGCGTTCAGAGAATGCAGATAACATGATTGATATGTTCAAAAAATCCCGTAGTGA
AGGATTTGGAGAAGAAGTAAAGCGTCGTATTATGTTAGGAACCTTTGCGCTTAGCTCTGGTTACTATGATGCTTATTATA
AAAAAGCACAAAAAGTACGTACATTAATAAAAAATGATTTTGATAAAATTTTAGAGGAATATGATGTTATCGTTGGGCCA
ACAACTCCAACTCCAGCATTTAAAGTCGGAGAAAAAACAAGTGATCCATTAACGATGTATGCGAACGACATTTTAACTAT
TCCAGTTAACTTAGCTGGTGTACCAGGAATTTCTATTCCATGTGGATTCTCTACAGAAGGTCTACCTATCGGGTTACAAA
TTATAGGTAACTATTTCGATGAAAGCACAGTATACCGTACAGCTTACGCATATGAACAAGCAACGGAACATCACAAGAAA
AGACCTCAACTTGGAGGTGCAAAATAA

Upstream 100 bases:

>100_bases
AAATGGATAAGCCAGGAAGACGCATTGCGTAATGCTCCTGATCATAAAGATGGTCAAATTCGTGTTCCATCGATATTAGA
ATAGGGAGGAGAAAATCATT

Downstream 100 bases:

>100_bases
TGAATTTCGAAACAATAATAGGTCTAGAAGTACACGTAGAATTAAAAACAAACTCAAAGATTTTTAGCCCAAGTACAAAT
GAATTTGGCTCTGATCCGAA

Product: aspartyl/glutamyl-tRNA amidotransferase subunit A

Products: NA

Alternate protein names: Glu-ADT subunit A

Number of amino acids: Translated: 488; Mature: 487

Protein sequence:

>488_residues
MSLFDYTIKELEEKLHNKEITVEDLVKESYQRIHEVDQEVHAFLTLDEEKALEKARELDQLEDKTGILFGMPGGIKDNIV
TKGLRTTCASQFLDNFNDPLYDATVVQKLQKENMVAVGKLNMDEFAMGSSNENSGYEPTRNPWNTEHVPGGSSGGSAAAV
AAGEVLFTLGSDTGGSIRQPAAFCGVVGMKPTYGRVSRFGLVAFASSLDQIGPVTRTVEDNARVLEVIAGHDQMDTTSAN
VEVPSYTEALKQDVKGLKIAVPKEYLAEGVSKEVKDAILEALKVYESMGATWEEVSLPHSKYALATYYLLSSSEASANLA
RFDGVRYGVRSENADNMIDMFKKSRSEGFGEEVKRRIMLGTFALSSGYYDAYYKKAQKVRTLIKNDFDKILEEYDVIVGP
TTPTPAFKVGEKTSDPLTMYANDILTIPVNLAGVPGISIPCGFSTEGLPIGLQIIGNYFDESTVYRTAYAYEQATEHHKK
RPQLGGAK

Sequences:

>Translated_488_residues
MSLFDYTIKELEEKLHNKEITVEDLVKESYQRIHEVDQEVHAFLTLDEEKALEKARELDQLEDKTGILFGMPGGIKDNIV
TKGLRTTCASQFLDNFNDPLYDATVVQKLQKENMVAVGKLNMDEFAMGSSNENSGYEPTRNPWNTEHVPGGSSGGSAAAV
AAGEVLFTLGSDTGGSIRQPAAFCGVVGMKPTYGRVSRFGLVAFASSLDQIGPVTRTVEDNARVLEVIAGHDQMDTTSAN
VEVPSYTEALKQDVKGLKIAVPKEYLAEGVSKEVKDAILEALKVYESMGATWEEVSLPHSKYALATYYLLSSSEASANLA
RFDGVRYGVRSENADNMIDMFKKSRSEGFGEEVKRRIMLGTFALSSGYYDAYYKKAQKVRTLIKNDFDKILEEYDVIVGP
TTPTPAFKVGEKTSDPLTMYANDILTIPVNLAGVPGISIPCGFSTEGLPIGLQIIGNYFDESTVYRTAYAYEQATEHHKK
RPQLGGAK
>Mature_487_residues
SLFDYTIKELEEKLHNKEITVEDLVKESYQRIHEVDQEVHAFLTLDEEKALEKARELDQLEDKTGILFGMPGGIKDNIVT
KGLRTTCASQFLDNFNDPLYDATVVQKLQKENMVAVGKLNMDEFAMGSSNENSGYEPTRNPWNTEHVPGGSSGGSAAAVA
AGEVLFTLGSDTGGSIRQPAAFCGVVGMKPTYGRVSRFGLVAFASSLDQIGPVTRTVEDNARVLEVIAGHDQMDTTSANV
EVPSYTEALKQDVKGLKIAVPKEYLAEGVSKEVKDAILEALKVYESMGATWEEVSLPHSKYALATYYLLSSSEASANLAR
FDGVRYGVRSENADNMIDMFKKSRSEGFGEEVKRRIMLGTFALSSGYYDAYYKKAQKVRTLIKNDFDKILEEYDVIVGPT
TPTPAFKVGEKTSDPLTMYANDILTIPVNLAGVPGISIPCGFSTEGLPIGLQIIGNYFDESTVYRTAYAYEQATEHHKKR
PQLGGAK

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family

Homologues:

Organism=Homo sapiens, GI222831590, Length=498, Percent_Identity=38.5542168674699, Blast_Score=312, Evalue=4e-85,
Organism=Homo sapiens, GI195972892, Length=498, Percent_Identity=26.3052208835341, Blast_Score=132, Evalue=1e-30,
Organism=Homo sapiens, GI166795287, Length=309, Percent_Identity=27.5080906148867, Blast_Score=74, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI17543272, Length=463, Percent_Identity=36.5010799136069, Blast_Score=267, Evalue=7e-72,
Organism=Caenorhabditis elegans, GI17537465, Length=486, Percent_Identity=26.1316872427983, Blast_Score=129, Evalue=2e-30,
Organism=Caenorhabditis elegans, GI17556264, Length=237, Percent_Identity=29.1139240506329, Blast_Score=99, Evalue=5e-21,
Organism=Caenorhabditis elegans, GI17538252, Length=422, Percent_Identity=23.696682464455, Blast_Score=94, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI71990152, Length=312, Percent_Identity=26.6025641025641, Blast_Score=93, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI17538254, Length=296, Percent_Identity=28.7162162162162, Blast_Score=81, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17556276, Length=210, Percent_Identity=32.3809523809524, Blast_Score=71, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17556278, Length=210, Percent_Identity=32.3809523809524, Blast_Score=71, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6323950, Length=464, Percent_Identity=36.6379310344828, Blast_Score=260, Evalue=4e-70,
Organism=Saccharomyces cerevisiae, GI6319685, Length=453, Percent_Identity=30.242825607064, Blast_Score=125, Evalue=1e-29,
Organism=Drosophila melanogaster, GI24648113, Length=495, Percent_Identity=35.3535353535354, Blast_Score=298, Evalue=5e-81,
Organism=Drosophila melanogaster, GI24652985, Length=486, Percent_Identity=26.1316872427983, Blast_Score=130, Evalue=2e-30,
Organism=Drosophila melanogaster, GI19922090, Length=486, Percent_Identity=26.1316872427983, Blast_Score=130, Evalue=2e-30,
Organism=Drosophila melanogaster, GI24652981, Length=486, Percent_Identity=26.1316872427983, Blast_Score=130, Evalue=2e-30,
Organism=Drosophila melanogaster, GI24652983, Length=486, Percent_Identity=26.1316872427983, Blast_Score=130, Evalue=2e-30,
Organism=Drosophila melanogaster, GI24644968, Length=505, Percent_Identity=26.3366336633663, Blast_Score=128, Evalue=9e-30,
Organism=Drosophila melanogaster, GI161078093, Length=510, Percent_Identity=27.0588235294118, Blast_Score=125, Evalue=5e-29,
Organism=Drosophila melanogaster, GI45550774, Length=478, Percent_Identity=24.2677824267782, Blast_Score=105, Evalue=7e-23,
Organism=Drosophila melanogaster, GI24648435, Length=478, Percent_Identity=24.2677824267782, Blast_Score=105, Evalue=8e-23,
Organism=Drosophila melanogaster, GI24648437, Length=478, Percent_Identity=24.2677824267782, Blast_Score=105, Evalue=8e-23,
Organism=Drosophila melanogaster, GI21356731, Length=480, Percent_Identity=24.1666666666667, Blast_Score=102, Evalue=8e-22,
Organism=Drosophila melanogaster, GI24648441, Length=412, Percent_Identity=24.2718446601942, Blast_Score=87, Evalue=4e-17,
Organism=Drosophila melanogaster, GI24648439, Length=412, Percent_Identity=24.2718446601942, Blast_Score=87, Evalue=4e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GATA_OCEIH (Q8ES78)

Other databases:

- EMBL:   BA000028
- RefSeq:   NP_691686.1
- ProteinModelPortal:   Q8ES78
- SMR:   Q8ES78
- GeneID:   1016295
- GenomeReviews:   BA000028_GR
- KEGG:   oih:OB0765
- NMPDR:   fig|221109.1.peg.773
- HOGENOM:   HBG481888
- OMA:   RWGLIAF
- ProtClustDB:   PRK00012
- BioCyc:   OIHE221109:OB0765-MONOMER
- GO:   GO:0006412
- HAMAP:   MF_00120
- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412
- Gene3D:   G3DSA:3.90.1300.10
- PANTHER:   PTHR11895
- TIGRFAMs:   TIGR00132

Pfam domain/function: PF01425 Amidase; SSF75304 Amidase_sig_enz

EC number: 6.3.5.-

Molecular weight: Translated: 53588; Mature: 53457

Theoretical pI: Translated: 4.80; Mature: 4.80

Prosite motif: PS00571 AMIDASES

Important sites: ACT_SITE 76-76 ACT_SITE 152-152 ACT_SITE 176-176

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLFDYTIKELEEKLHNKEITVEDLVKESYQRIHEVDQEVHAFLTLDEEKALEKARELDQ
CCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHEECHHHHHHHHHHHHH
LEDKTGILFGMPGGIKDNIVTKGLRTTCASQFLDNFNDPLYDATVVQKLQKENMVAVGKL
HHCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCEEEEECC
NMDEFAMGSSNENSGYEPTRNPWNTEHVPGGSSGGSAAAVAAGEVLFTLGSDTGGSIRQP
CHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHCEEEEEECCCCCCCCCCC
AAFCGVVGMKPTYGRVSRFGLVAFASSLDQIGPVTRTVEDNARVLEVIAGHDQMDTTSAN
HHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHCCCHHHHHHHHCCCCCCCCCCC
VEVPSYTEALKQDVKGLKIAVPKEYLAEGVSKEVKDAILEALKVYESMGATWEEVSLPHS
EECCHHHHHHHHHHCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCH
KYALATYYLLSSSEASANLARFDGVRYGVRSENADNMIDMFKKSRSEGFGEEVKRRIMLG
HHHHHHHHHHCCCCCCCHHHHHCCEEECCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHH
TFALSSGYYDAYYKKAQKVRTLIKNDFDKILEEYDVIVGPTTPTPAFKVGEKTSDPLTMY
HHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCEEECCCCCCCCCCCCCCCCCCEEEE
ANDILTIPVNLAGVPGISIPCGFSTEGLPIGLQIIGNYFDESTVYRTAYAYEQATEHHKK
ECCEEEEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHC
RPQLGGAK
CCCCCCCC
>Mature Secondary Structure 
SLFDYTIKELEEKLHNKEITVEDLVKESYQRIHEVDQEVHAFLTLDEEKALEKARELDQ
CHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHEECHHHHHHHHHHHHH
LEDKTGILFGMPGGIKDNIVTKGLRTTCASQFLDNFNDPLYDATVVQKLQKENMVAVGKL
HHCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCEEEEECC
NMDEFAMGSSNENSGYEPTRNPWNTEHVPGGSSGGSAAAVAAGEVLFTLGSDTGGSIRQP
CHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHCEEEEEECCCCCCCCCCC
AAFCGVVGMKPTYGRVSRFGLVAFASSLDQIGPVTRTVEDNARVLEVIAGHDQMDTTSAN
HHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHCCCHHHHHHHHCCCCCCCCCCC
VEVPSYTEALKQDVKGLKIAVPKEYLAEGVSKEVKDAILEALKVYESMGATWEEVSLPHS
EECCHHHHHHHHHHCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCH
KYALATYYLLSSSEASANLARFDGVRYGVRSENADNMIDMFKKSRSEGFGEEVKRRIMLG
HHHHHHHHHHCCCCCCCHHHHHCCEEECCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHH
TFALSSGYYDAYYKKAQKVRTLIKNDFDKILEEYDVIVGPTTPTPAFKVGEKTSDPLTMY
HHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCEEECCCCCCCCCCCCCCCCCCEEEE
ANDILTIPVNLAGVPGISIPCGFSTEGLPIGLQIIGNYFDESTVYRTAYAYEQATEHHKK
ECCEEEEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHC
RPQLGGAK
CCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12235376